Purchase of LCMSMS for DoAFW, Punjab

PUNJAB BIOTECHNOLOGY INCUBATOR

COMMERCIAL BID FORMAT

Name of the Equipment Liquid Chromatography Mass Spectrometry (LC-MS/MS)

Name of the Manufacturer

Make of the Equipment

Country of Origin

Name of Bidders

Part A - Main Equipment

Application Rate (In Rs.) GST Amount Total Amount (Rs.)

High sensitivity trace level detection and quantification of Pesticide residues in agriculture based commodities and products of allied sectors as per National/International requirements.

Mass Spectrometer System coupled with Liquid Chromatography must be quoted with all the required accessories & parts capable of meeting the above listed applications. The system must meet the following specifications:

Mass Spectrometry

Mass range 10-2000 amu or better.

Mass Resolution 0.6 unit mass resolution or better

Mass Accuracy Better than 0.1 Dalton.

Mass Stability 0.1 Dalton over a period of 24 hours or better.

Mass Scan Speed 12000 amu/s or better

Sensitivity MRM Sensitivity:

1pg Reserpine/ Chloramphenicol on column should be able to produce minimum S/N > 700000:1 or better in both positive and negative mode

On column 10 fg injection of Reserpine/ Chloramphenicol with IDL < 2.8 fg in both positive and negative mode

Source Electrospray Ionization (ESI)

Desolvation Temperature ≥500°C (user selectable through software)

Linear Dynamic Range 5 orders or better

Pump and Port Programmable infusion pump with 1 mL gas tight syringe

Interface Must be capable to handle large batches of complex samples of food matrices over a long period of time without performance degradation. The instrument must have a proven atmosphere to vacuum interface.

Collision Cell Collision cell should have dwell time ≤ 5ms.

Polarity Switching ≤25 msec or better

Vacuum system A robust, high efficiency air cooled turbo molecular pump and suitable backup pumps with minimum maintenance. Vacuum system should be digitally monitored and controlled. Vacuum read backs and automated vent system in case of power failure.

Operating Modes Full Scan MS, Selected Ion Monitoring/ Recording (SIM/SIR), Product Ion Scan, Precursor Ion Scan, Neutral loss scan and MRM Scan or any additional advance mode.

Database and Library Latest version of NIST traceable library and database for ≥1000 compounds for Pesticides and other Environmental contaminants.

Software Complete system and software configuration must be 21 CFR Part 11 compliant.

· Windows Based software with multitasking and capable of performing the following functions: Control the MS, acquire, store, process and reproduce the data.

· It must be able to control all the devices from same software.

· Software should allow monitoring of one molecular ion and upto four confirmatory ions.

· Quantification software for batch process must confirm the analytes as per regulatory requirements in food sample analysis as per the applications specified.

· Workstation must be able to control the MS, acquire, store, process and reproduce the data by the same computer.

Tuning and calibration The system should be equipped with automatic tuning and calibration.

Communication Hardware: Branded Computer System with i5/ Xenon or better processor, 16 GB RAM or better and 2TB HDD or better, 22-23” Full HD Monitor with licensed windows compatible with equipment Software, MS office Latest standard version and Antivirus for 3 year. System should have minimum 2 network ports. Printer - B/W - duplex - laser - Legal, A4 - 1200 dpi x 1200 dpi - up to 21 ppm – capacity with Network Card and Bluetooth facility.

Liquid Chromatography System

Pump Binary/Quaternary pump

0.001 to 2.000 ml/min, in 0.001 ml increments with flow rate precision <0.2%RSD

Maximum Operating Pressure 15000 psi or more

Degasser Online degasser for all channels

Auto Sampler ≥ 96 samples vials (1.5/2ml)

Injection Volume Range 1-50 µl in 0.1ul increments sample delivery with injection precision <0.3% RSD

Injector compartment Autosampler should be thermo stated 4 to 40°c, programmable in 1°c increments.

Sample Carryover < 0.005%

Column Compartment Thermostated ambient to 80oC, programmable in 10oC increments

Capable of holding at least two (02) 15 cm columns

Compressor & Gas supply • Peak Scientific/OEM Nitrogen generator (1 no.) with an inbuilt compressor with a compatible flow rate to MS and purity of 99.5% or better

• High purity Gas cylinders (02no.) as per the instrument requirement along with all accessories, i.e., gas purification panel unit, regulators, SS tubing etc to make the system operational.

IQOQ On site IQ, OQ of instrument along with document.

Training Satisfactory Technical and Application training to the personnel at site immediately after its installation

Method Validation 1. Method validation of Pesticide Residues in Agri-food commodity in mutually agreed matrix as per FSSR/EU regulations including following anionic polar pesticides:

Glyphosate, Glufosinate, AMPA, Ethephon, FosetylAl, Phosphonic acids, chlorate & Perchlorate.

The method should be with direct injection based without derivatization at default MRL’s in single run (documentary evidence to be submitted).

1. Manpower (operator): The supplier of the equipment shall depute Scientific/Technical staff after installation (Minimum Science Post-Graduate with 6 months experience on the similar equipment) for one (01) year to make the system operational and carry out validations.

2. The consumables (chemicals, plastic/glassware, reference material-NIST/ISO-17034, etc.) required for method validation shall be in the scope of supplier/vendor and shall be supplied before the commencement of method validation, keeping in view their expiry period

3. The method validation activity shall have to be completed on the system installed at PRTL, Jalandhar within six (06) months of successful installation & commissioning of instrument, failing which LD/Penalty clause of Tender Document will be applicable

Pre Installation Provide PIR of the system as per the form at given at Annexure J of tender document.

Total Amount of Part A

PART B - OTHER REQUISITES FOR LCMSMS

OTHER REQUISITES FOR LCMSMS Rate (In Rs.) GST Amount Total Amount (Rs.)

Complete Gas Purification Panel with fittings & installation of required gases

Renewable In Line Gas Purification System with Spare Set of gas purifier cartridge

Gas clean filters/Traps (3 No.)

Columns C18=Poroshell120 SB100 X3mm,2.7 μm or equivalent - 02 no.

C18 = Poroshell120 EC 100 X 4.6mm, 2.7μm or equivalent - 02no.

C18 = 100 X 4.6mm, 3.5μm, or equivalent - 02 no.

C18 =50X4.6mm,2.7μm,orequivalent - 02no.

Any other specific column as per Method Validation requirement – 02 no.

Vials, caps · Vial 1-2ml with split Septa PTFE/Silicone screw caps, Amber glass - 2000No.

(Only Compatible sizes should be supplied) · Vial 1-2ml with split Septa PTFE/Silicone screw caps, Clear glass - 2000No.

· Polypropylene/Polyethylene/PEEK Vial set 1-2ml, - 2000No.

· 300/500 µL Recovery vials -250 No.

· Septa PTFE/Silicone (for 1-2 mL Vials) - 2500 No.

· Ergonomic Crimping Tools for different vial types

· Ergonomic Decapping Tools for different vial types

· 5 No. each Storage Racks for (for 1-2 mL Vials)

Accessories · Solvent filters (Glass & SS both , 08 no. each)

Total Amount of Part B

PART C - Extended Warranty Charges (After expiry of Standard Warranty of One (01) Year

1st Year Extended Warranty Charges

2nd Year Extended Warranty Charges

3rd Year Extended Warranty Charges

4th Year Extended Warranty Charges

5th Year Extended Warranty Charges

6th Year Extended Warranty Charges

Total Amount of Part C

PART D - Optional Items

Micropipettes Micropipette set with variable volumes:- Pipettes of 1-10µl, 10-100 µl, 20-200 µl, 10-1ml, 10ml volume 02 no. each with tips – 500 no. per size each

Sample Homogenizer Sample homogenizer (electrically operated) based on rotor blade principal for grinding high moisture, high fat and fibrous samples, should have capacity of 4.5 litre or better, brushed stainless steel bowl, Fine Serrated blade, Polycarbonate see-through lid, high speed of 3000 rpm or better, during grinding-temperature should not increase so that analyte not get degraded, Motor built on ball bearings for silent running without vibration, Direct drive motor.

Manual Solid Phase Extractor Manual Solid Phase Extractor with vacuum pump (01 no.): Capable to process 12 samples or better simultaneously with collection vessel rack size 16mm x 100mm tubes. Should have Vacuum gauge with control and release valves for monitoring and adjustment of vacuum level.

Computer for data processing i5/ Xenon or better processor, 16 GB RAM or better and 2TB HDD or better, 22-23” Full HD Monitor with licensed windows compatible with equipment Software, MS office Latest standard version and Antivirus for 3 year. System should have minimum 2 network ports. Printer - B/W - duplex - laser - Legal, A4 - 1200 dpi x 1200 dpi - up to 21 ppm – capacity with Network Card and Bluetooth facility.

Total Amount of Part D

Total FOR Amount for One (01) Unit upto PBTI Site- Part A + Part B + Part C + Part D

Total Amount in Words: Rupees

Total FOR Amount for Two (02) Unit upto PBTI Site- Part A + Part B + Part C + Part D

1. It is mandatory to quote item wise rates for Part B & Part C, Part D lump sum rates are not acceptable for these.

2. Bidder must quote amount in the given cells, if amount against any cell is included in main component, bidder should put "Zero" value.

3. Taxes must be mentioned, if it is not mentioned, the same shall be treated as included in given rates.

4. This is to certify that the rates quoted above are not more than the rates charged from any Institution/Department/Organization

5. Keeping in view the availability of Funds, optional items can be included in purchase order.

6. Financial Comparison shall be carried out based upon the FOR value of the equipment including the cost of Extended Warranty for six (06) years after expiry of Standard Warranty and optional items & other obligations.

Signatory Name:

List of consumables required for trouble free operation of equipment which are not covered under Standard Warranty and Extended Warranty

Sr. No. Name Qty. Rate

Remarks, if any:-

Name of Company

Name of Authorzed Signatory