Equipments for the ICMR NER Task Force New Scheme entitled Advanced Mycology Diagnostic and Research Centre

Items :

Real-Time PCR Specifications: An automated system for both PCR and post-PCR end-point analysis, using in-built Peltier based PCR machine; The system should support applications including absolute and relative quantification, multiplex PCR allelic discrimination, melt curve analysis, pathogen detection and plus/ minus assays using an internal positive control; Peltier thermal cycling for Fast-PCR as well as Standard-PCR run available in the same block. 40 cycles in less than 30 minutes; The system should have temperature range of 3-99 degrees C with Maximum ramp rate of at least 5°C /second; Should have a reaction volume of at least 50 µl; The system should support micro well plates, individual tubes and 8-tube strips; The system should provide Touch Screen LCD feature to avoid dependency on computer for operation; Should also be possible to use a computer for system control, operation, analysis and networking of multiple system; Instrument should be supplied with a separate Desktop along with a standalone backup system capable of providing at least 90 minutes of backup time; USB port for data export to Power point, Excel or JPEG formats should be present; It should be an open system to use reagents from any manufacturer; System should be calibrated for common dyes of broader wavelength excitation and emission spectrum; Excitation Light source –Laser / LED; Change of position, block or chemistry should not require calibration or tools; The system should be capable of operating at ambient temperatures of 20-30 degrees C; Temperature gradient function should be available in the system; Temperature uniformity should be ± 0.15°C & control accuracy ± 0.1°C; Temperature increments should be min. of 0.1 °C/cycle & Time increments should be min. 1 s/cycle; It should have at least 5 Chanel multiplexing capability in a single tube; Instrument should not require the use of a reference dye such as ROX and do not need regular calibration; Instrument should use an advanced optical system; It should do Scanning just above the sample & should guarantees the homogeneous excitation of each individual sample and the reliable recording of the emitted fluorescent signals with high sensitivity and no crosstalk between wells. Should be European CE approved. Should have a warranty period of at least 3 years and should include on-site installation and training.

Minus 20°C Deep Freezer Specifications: The deep freezer should have atleast 250 Ltrs of capacity; Should have Temperature control system with microprocessor PID control; Control of temperature within 3 °C of set temperature; Doors should have triple gasket system to prevent any leakage; Doors should have polyurethane insulation; Should have Audio Alarm System, in case of i) high and low temperature ii) open doors iii) Power failure; Should provide onsite installation & training; Should have at least 3 years warranty

M B Traders

B. M. Pharma