TENDER FOR SUPPLY OF CHEMICAL AND REAGENTS FOR MDRU DEP

ITEM NAME

MDRU Kits and reagents

Ammonium chloride 500gm

Potassium bi carbonate 500gm

Sodium chloride 500gm

Tris HCL 500gm

SDS 500gm

Saturated Phenol 500ml

Chloroform 500ml

Sodium acetate 250gm

Isoamyl alcohol 500ml/1ltr

Glacial acetic acid 500ml/1ltr

Molecular Biology Grade Agarose Powder 250gm

Bromophenol blue dye 2ml*5=1pack

Ethidium bromide 10ml

Molecular Weight (DNA Ladder) 100bp & 1Kb 1 vial

Molecular Weight (DNA Ladder) 50bp 1 vial

Molecular Weight (DNA Ladder) 25bp 1 vial

Taq polymerase 500units/vial

AmpliTaq Gold DNA Polymerase master mix 500units/vial

MgCl2 5ml

dNTP Mix 1ml

DNase 1000 unit

RNase 1000 unit

Proteinase K 1000 unit

Tris-EDTA 500 gm

EDTA 250gm/500gm

Boric Acid 250gm/500gm

Teepol 5 liter

Xylene Cynol 10 gm

DMSO 50 ml

Tips i. 0.2 - 20 µl tips

Superscript II RNase Reverse Transcriptase/ EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT) 400/500 reaction pack.

Power SYBR Green PCR Master Mix 5 ml

Power SYBR Green RT PCR reagent Kit 5 ml

oligo (dT) 12-18 primer 25ug (0.5ug/ul)

Absolute Ethanol 500ml

PCR Plates (light cycler 480 compatible) pack of 50 / pack of 100

Sealing foil (RT PCR/qPCR grade) (light cycler 480 compatible) pack of 50 / pack of 100

Filter Tips Each pack contains 1000 psc.

MCT Variable tubes Each pack contains 1000 psc.

i. 20 - 200µl tubes

ii. 200 - 600 µl tubes

iii. 500 - 2000 µl tubes

Nitrile Autodextorous gloves Each pack contains 1000 psc.

MCT Stands for Variable tubes sizes Each pack contains 10 psc.

i. 20 - 200µl tubes Stand

ii. 200 - 600 µl tubes Stand

iii. 500 - 2000 µl tubes Stand

Filter Tip Boxes Each pack contains 10 psc.

i. 0.2 - 20 µl filter barrier tip Box

ii. 20 - 200 µl filter barrier tip Box

iii. 200 - 1000 µl filter barrier tip Box

RT PCR grade water pack size of 20ml (20 ml * 5)

Tip Discard Box (1-2 Liter capacity) Each

Graduated Measuring cylinders 50,100,500,1000 ml Each

Graduated Beakers 50,100,500,1000 ml Each

Flat Bottom Tube 5ml (With Screw Cap) Pack size of 500 psc.

Tube Stand (15ml Falcon, 5ml, 2ml, 0.5ml, 0.2ml MCT) Pack size of 10 psc.

Graduated Conical Flask 50,100,500,1000ml Pack size of 5 psc.

Test tube 5,10 ml Each pack contains 100 psc.

Slide + cover slips (25mm*75mm) Each pack contains 100 psc.

Tissue paper Roll Pack size of 12 psc.

Fine Tissue Cloth Roll Pack size of 12 psc.

Cotton Pack size of 10 psc.

Wash bottle/ Dropping Bottle,200ml,500ml,1Ltr each

Funnels Variable range each

Plastic bottle,200,500,1000ml each

Syringe + Needle 2 ml, 5 ml Pack size of 100 psc. each

Nitrile gloves; medium and large size Box Pack size of 1000 psc.

DNA isolation Kit {BLOOD} per kit

RNA isolation Kit per kit

Phenol 500 ml

HNO3 (Nitric Acid) 500 ml

Propionaldehyde pure (97%) 500 ml

Phthalic anhydride 500 ml

Glacial acetic acid AR 500 ml

Hydrochloric acid AR 500 ml

Sulfuric acid AR 500 ml

2-Amino ethanol 500 ml

Pyridine AR 500 ml

Ammonia solution AR 500 ml

Ammonia chloride AR 500 ml

Acetyl Salicylic acid 500 ml

Acetone AR 500 ml

Anthranilic acid AR 500 ml

Activated charcoal 500 ml

Silica Gel G 500 ml

Benzoic acid AR 500 ml

SDS 500 gm

Colin (Cleaning detergent solution) 500 ml

Sterilium (Hand Sanitizer) 100 ml * 5

Dettol / Lifeboy alcohol based hand Sanitizer 100 ml * 5

Floor cleaner phenyl 1 l * 5

Cleaning Mop per psc

Broom per psc

Microwave gloves per pair

Brown paper for autoclaving per roll

liquid nitrogen 10/25 ltr.

Phosphate buffer saline (10x; pH 7.4; RNase free) 500 ml

Formalin (Formaldehyde aqueous solution; Lab grade) 500 ml

Paraffin wax (58-600C for Histology) 500 gm

Xylene (Molecular Lab Grade) 500 ml

Glycerol 500 ml

Ammonia (NH4OH; Extra Pure) 250 ml / 500 ml

Methanol ( Methyl alcohol, CH3OH) 500 ml

Acrylamide/Bis AR 500 ml

10X TBE Buffer 500 ml

Urea (Ultra pure; Mol-bio grade) 500 gm / 1Kg

Ammonium Persulfate 100 gm

TEMED (Ultra pure; Mol-bio grade) 100 ml / 250 ml

4’,6-diamidino-2-phenylindole 50 ml

Diethyl pyrocarbonate 5 gm / 25 gm

TAE Buffer

Sybr Gold 100ul

Restriction Enzyme – Mnl I 250 / 300 / 500 units

Restriction Enzyme – BclI 1000 / 1500 / 2500 / 3000 units

Restriction Enzyme – HpyCH4V 100 / 500 units

Restriction Enzyme – HpyCH4III 200 / 250 / 1000 / 1250 units

Restriction Enzyme – Sau96I 1000 units

Restriction Enzyme – SfcI 200 / 1000 units

Restriction Enzyme – BccI 1000 units

Restriction Enzyme – ScrFI 500 / 1000 / 2500 units

Restriction Enzyme – AflIII 250 / 1250 units

Restriction Enzyme – ScaI 500 / 1000 / 1250 units

Restriction Enzyme – AvaI 1000 / 2000 units

Restriction Enzyme – BsmI 200 / 500 / 1000 / 2500 units

Restriction Enzyme – TspRI (also share cleavage site with TscAI) 1000 units

Restriction Enzyme – MboII 250 / 300 / 1250 / 1500 units

Restriction Enzyme – Bsh1236I 500 / 1000 / 2500 units

Restriction Enzyme – BanII 1000 / 1500 / 2000 units

Restriction Enzyme – Mph1103I 1000 / 5000 units

Restriction Enzyme – Dde I 200 / 500 / 1000 / 2500 units

Restriction Enzyme – BsmB I (also share cleavage site with Esp3I) 200 / 400 / 1000 units

Restriction Enzyme – Afa I (also share cleavage site with Rsa I) 1000 / 5000 units

Restriction Enzyme – Bal I (also share cleavage site with Mlu NI) 50 / 100 / 200 / 250 units

Restriction Enzyme – FspI (also share cleavage site with NsbI) 400 / 500 / 1000 / 2500 units

Restriction Enzyme – Hpa II (also share cleavage site with MspI) 1000 / 2000 / 4000 / 5000 / 10000 units

Restriction Enzyme-Hinf I

Restriction Enzyme- HpyCH4

Restriction Enzyme-MboII

Restriction Enzyme-BstUI

Restriction enzyme- MvaI

Primers

FMR1 set 1 –F5'- TCAGGCGCTCAGCTCCGTTTCGGTTTCA-3' R5'-5 AAGCGCCATTGGAGCCCCGCACTTCC-3'

MECP2 Exon 1 set 1- F5'-GTTATGTCTTTAGTCTTTGG–3´ R5'-TGTGTTTATCTTCAAAATGT–3´

Exon 2 set 1-F5'-CCTGCCTCTGCTCACTTGTT–3´ R5'- GGGGTCATCATACATGGGTC–3´

Exon 2 set 2- F5'-AGCCCGTGCAGCCATCAGCC–3´ R5'- GTTCCCCCCGACCCCACCCT–3´

Exon 3 set 1 –F5'-TTTGTCAGAGCGTTGTCACC–3´ R5'- CTTCCCAGGACTTTTCTCCA–3´

Exon 3 set 2- F5'-AACCACCTAAGAAGCCCAAA–3´ R5'- CTGCACAGATCGGATAGAAGAC–3´

Exon 3 Set 3- F5'-GGCAGGAAGCGAAAAGCTGAG–3´, R5'- TGAGTGGTGGTGATGGTGGTGG–3´

Exon 3 Set 4 – F5'-5´–TGGTGAAGCCCCTGCTGGT–3´ R5'- CTCCCTCCCCTCGGTGTTTG–3´

Exon 3 set 5- F5'GGAGAAGATGCCCAGAGGAG–3´ R5'- CGGTAAGAAAAACATCCCCAA–3´

Exon3 (L100V)- F5'-AACCACCTAAGAAGCCCAAA-3' R5'- GCTTAAGCTTCCGTGTCCAGCCTTCAGGTA-3'

Putative promoter and Exon 1. - F5'-GGGTGCAATGAAACGCTTA-3' R5'- TTTACCACAGCCCTCTCTCC-3'

MC4R rs17782313 - F 5'-AAGTTCTACCTACCATGTTCTTGG-3' R 5'-TTCCCCCTGAAGCTTTTCTTGTCATTTTGAT-3' FTO rs9939609 -F 5'-AACTGGCTCTTGAATGAAATAGGATTCAGA-3' R5'-AGAGTAACAGAGACTATCCAAGTGCAGTAC-3'

ADIPOQ rs2241766 – F5'- TGTGTGTGTGGGGTCTGTCT-3' R-5' -TGTGATGAAAGAGGCCAGAA-3'

rs1501299- F5'- CTACACTGATATAAACTATATGGAG-3' R5'-CCCCAAATCACTTCAGGTTG-3'

PCSK1 rs155971 – F5’TATATGCAGCCACCAATCCA 3’ R5’AAAATGAAGGGAGAAGCACAAA3’

POMC rs6232 - F5'- TTGTGCCCTTCATCTGAACA-3' R5'- TGTAGCAACTTTGGCATGGA-3'

rs155971 - F5'TATATGCAGCCACCAATCCA-3' R5'-AAAATGAAGGGAGAAGCACAAA-3'

PPAR-G (Pro12Ala) -F5'GCC AAT TCAAGC CCA GTC-3'R5'GAT ATGTTT GCA GAC AGT GTA TCA GTG AAGGAA TCG CTTTCC G-3'

KCNJ11 (rs5219) -F5'-GACTCTGCAGTGAGGCCCTA-3’ R5'-ACGTTGCAGTTGCCTTTCTT-3’

CAPN10 (rs3792267) -F5'-CACGCTTGCTGTGAAGTAATGC-3’R5'-TGATTCC CATGGTCTGTAGCAC-3’PIK3CA - set 1-Forward-5’-GGAGTATTTCATGAAACAAATGAATGATGCG-3’ Reverse- 5’-GAGCTTTCATTTTCTCAGTTATCTT-3’

BAT 25 -set 1- F 5’-TCGCCTCCAAGAATGTAAGT-3’R 5’-TCTGCATTTTAACTATGGCTC-3’BAT 26 -set 1-F5’-TGACTACTTTTGACTTCAGCC-3’R 5’-AACCATTCAACATTTTTAACCC-3’

D2S123 -set 1- F5’-AAACAGGATGCCTGCCTTTA-3’ R5’-GGACTTTCCACCTATGGGAC-3’

D5S346-set 1- F 5’-ACTCACTCTAGTGATAAATCGGG-3’ R 5'-AGCAGATAAGACAGTATTACTAGTT-3'

D17S250 – set 1- F 5’-GGAAGAATCAAATAGACAAT-3’ R5’-GCTGGCCATATATATATTTAAACC-3’

IMPDH2 set 1- F5′- GTTTCTGCGGTATCCCAATC -3′ R5′- CGAGCAAGTCCAGCCTAT-3′ BMP6 - rs73719353- F5’-GCTCCTTTGCACTTCGCTGT-3’

R5’-AGGCTCTGCTG AGCTCCTAC-3’

BMP6- rs73719341- F 5’TGAACTTCCCATTCCCCTCT-3’ R5’ATAAAATTAGCATTGATCCA-3’

BMP6- rs73719318 F 5’CAGGTGCTGTGCAACTTCTT-3’ R 5’AGAGGGCACCATGGTTGCCT-3’

BMP6-rs73381662 F 5’-CTGAGATTCAATTAGGCCCA-3’ R 5’TAAAGAACAGCAAAAGTCTG-3’

BMP6-rs73381650 F 5’CACATAAAGATTGCTGCATT-3’ R 5’TAGTAATCCTAAAAATGGGA-3’

ANXA2- rs7170178 F 5’-TTCACAGCAGTTCAAAATAC-3’ R 5’-CTGGGTTTCCAGAGATGGAA-3’

ANXA2 - rs73435133 F 5’-GAGTGCAAGGTGCTGAGGAT-3’ R 5’-GATTTCAGACAGCCCTTGCA-3’

ANXA2- rs73418020 F 5’-TCTGAGAGTGAAAGGTGCAC-3’ R 5’-TCCCATCCCCTGAATCCCTG-3’

ANXA2- rs72746635 F 5’-CCTGACTCATTGTCACATCA-3’ R 5’-AAGTGGCTTTCCACTGCCC-3’

ANXA2- rs73418025 F 5’- CTTCTCATCTTACTTTT-3’ R 5’- AGGGAAGGATACAGAGGAGA-3’

HSP-70 primer sequence 5'-AGCGT AACAC CACCA TTCC-3' (forward) 5'-TGGCT CCCAC CCTAT CTC-3' (reverse)

The GAPDH sequence Forward primer 5'-AGC CAC ATC GCT GAG ACA C-3', Reverse primer 5'- GCC CAA TAC GACCAA ATCC-3'.

MTHFR F:5′-TGTGGTCTCTTCATCCCTCGC-3′; R: 5′-CCTTTTGGTGATGCTTGTTGGC-3′.

DPYD F:5'-ACTCAATATCTTTACTCTTTCATCAGGAC-3'. R: 5'-ACATTCACCAACTTATGCCAATTCT-3'.

TYMS F:5’-GGTACAATCCGCATCCAACTATTA-3’ R:5’-CTGATAGGTCACGGACAGATTT-3’

IMP3 Forward:5’ATGACTCCTCCCTACCCG3’ Reverse:5’GAAAGCTGCTTGATGTGC3’

CXCL1 Forward: 5’CCAGACCCGCCTGCTG-3’and Reverse:5’CCTCCTCCCTTCTGGTCAGTT-3’

COX-2 Forward: 5′- CAGCCATACAGCAAATCC -3′; Reverse: 5′-TCGCACTTATACTGGTCAA-3′

hMLH1 F-5′ TTT TGA TGT AGA TGT TTT ATT AGG GTT GT 3′R-5′ ACC ACC TCATCATAA CTA CCC ACA 3′

PPAR-G (Pro12Ala) -

F5'GCC AAT TCAAGC CCA GTC-3'

R5'GAT ATGTTT GCA GAC AGT GTA TCA GTG AAGGAA TCG CTT TCC G-3'

Methylated(hMLH1) - F-5′ ACG TAGACG TTT TAT TAG GGT CGC 3 R- 5′ CCT CATCGTAAC TAC CCG CG 3

hMSH2 - F-5′ GGT TGT TGT GGT TGG ATG TTG TTT 3 R- 5′ CAA CTA CAA CAT CTC CTT CAA CTA CAC CA 3'

Methylated(hMSH2) - F- 5′ TCG TGG TCG GAC GTC GTT C 3' R-5′ CAA CGT CTC CTT CGA CTA CAC CG 3′

β-actin: Forward: 5’-CTACGTCGCCCTGGACTTCGAGC-3’ ß-actin: Reverse: 5’-GATGGAGCCGCCGATCCACACGG-3’

KRAS-Forward: 5′-GACTGAATATAAACTTGTGGTAGTTGGACCT-3′.Reverse: 5′-CTATTGTTGGATCATATTCGTCC-3′.

BRAF- Forward: 5′-TCATAATGCTTGCTGATAGGA-3′. Reverse: 5′-GGCCAAAAATTTAATCAGTGGA-3′.

MTHFR(C677T)‘‘5'-GCACTTGAAGGAGAAGGTGTC-3'” and reverse primer ‘‘5'-AGGACGGTGCGGTGAGAGTG-3'”

MTHFR(A1298C) forward ‘‘5'-CTT TGG GGA GCT GAA GGA CTA CTA C-3'” and reverse ‘‘5'-CAC TTT GTG ACC ATT CCG GTT TG-3'” primers.

Total RNA isolation mini kit (from human skin tissue) / RNeasy Fibrous Tissue Mini Kit (For RNA Extraction from human skin tissue) (Qiagen) Per Kit (each Kit pack is for 50 reactions)

PuroSPIN™ Fibrous Tissue RNA Purification Kit (Luna Nanotech) (For RNA Extraction from human skin tissue) Per Kit (each Kit pack is for 250 reactions)

Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Biorad) / MP Biomedical's FastRNA Pro Green Kit Per Kit (each Kit pack is for 50 reactions)

Human leptin ELISA kit Per Kit (each Kit pack is for 96 reactions)

Human adiponectin ELISA Kit Per Kit (each Kit pack is for 96 reactions)

Human adipsin ELISA Kit Per Kit (each Kit pack is for 96 reactions)

Human Resistin ELISA Kit Per Kit (each Kit pack is for 96 reactions)

Human Iron ELISA Kit (serum iron) Per Kit (each Kit pack is for 96 reactions)

Human Ferritin ELISA Kit (serum/ ferritin) Per Kit (each Kit pack is for 96 reactions)

GDF15 human Elisa Kit Per Kit (each Kit pack is for 96 reactions)

Spexin human Elisa Kit Per Kit (each Kit pack is for 96 reactions)

Human PAI-1-ELISA Kit Per Kit (each Kit pack is for 96 reactions)

Thyroid Estimation Kit Per Kit (each Kit pack is for 96 reactions)

Ice maker machine for laboratory purpose 1 unit

Microwave gloves Each packet contains one pair of gloves.

PCR mini cooler / coolcube microplate and pcr tube cooler each

Horizontal gel apparatus: 18 – 20 cm (Length) x 25 – 30 (Breadth) x 5- 7.5 cm (Height), 40-60 samples, multichannel pipette compatible combs and gel caste each

Mini-Horizontal gel apparatus: 9 cm W x 11 cm L with grooves (8.7 cm L x 1.2 cm H) on the side for gripping the gel tray. It should have two comb slots on the same tray area. Buffer capacity should be 600 ml for the buffer tanks and optimum gel runs with a fill line indicator for buffer levels along the unit side each

multi size Forceps lab set Each packet contains multi size Forceps lab set

Liquid nitrogen sample storage tanks Each

liquid nitrogen sample handling gloves Each packet contains one pair of gloves.

L-mold each

Tissue cassette steel each

Electric Tissue float bath (thermostate) each

Coupling jar each pack contains 2 psc.

Staining rack each

Whatman filter paper grade 1 & 2 Each packet contains 50 psc..

Harri’s Hematoxylin powder 25 / 50 / 100 / 250 / 500 gm

Yellow Eosin powder 25 / 50 / 100 / 250 / 500 gm

Coverslip 18x18 (microscopic) Each packet contains 100 psc..

DPX mount 100 ml / 250 ml

Hot plate each

MX35 Premier Microtome blade (34/80mm) 50 blades Each box contains 50 psc..

Diamond point marker pen (histopathology use) each

Embedding mold and embedding ring each

QIAmp DNA FFPE Tissue Kit (50 rxns)

Genomic DNA purification kit (Promega)

RNA extraction kit from Tissue

cDNA synthesis kit

Superscript II RNase Reverse Transcriptase

SYBR Green PCR Master mix

Sodium Bisulphite

PAGE loading dye

formamide

N’N’-methylene bisacrylamide

Ammonium persulfate

TEMED

Polyacrylamide

Wizard DNA clean up system (Promega)

2-Mercaptoethanol

Silver stain

hydroquinone

Blotting paper

DNA ladder 10 bp

PAS Stain

Histopathology Plastic Cassettes

Poly – L – Lysine Coated Slides

Deep well Mortar and pestle homogenizers (medium size)

Deep well Mortar and pestle (small size)

RNeasy MiniElute Cleanup Kit

Phase – Lock Gel Heavy 5 Prime Phase – Lock Gel Heavy 5 Prime

Qiazol Lysis Reagent

RNeasy MiniElute Cleanup Kit

Cryo-Vial 1 Pkt contains 50 PSC.

Deep well Mortar and pestle (small size)

SCIENTIFIC TRADING CORPORATION