Supply and installation of Elispot Reader.

Indian Institute of Science Education and Research Bhopal

Annexure-I(b) Annexure-I(b)

Technical Compliance Sheet

Tender No.: IISERB/SP/2025-26/111 Dated 27.02.2025

Item - Supply & installation of Elispot Reader.

Sl. No. Specifications Make and offered Model Authorization certificate attached/Not attached Comply /Not Comply

1 Proposal for Advanced Cell-Based Immune Monitoring Facility

2 Imaging and Analysis Systems with control system and display for 6, 12, 24, 48, 96, 384, 1536 type plates with Multiplexing: Pragmatic and protective immunity upto 7 analytes

3 Green-Red-Blue-Violet illumination and 440,520,600,625,690 nm for DAPI, Alexa Fluor® 405, Pacific Blue™, CF™ 405, Po-Pro™, Sytox™ Blue,FITC, AO, CFSC, Alexa Fluor® 488, GFP, sfGFP, Sytox™ Green, Yo-Pro™,PE, Cy3, TRITC, PI, Sytox™ Orange, dsRed, mRuby2,PI, mCherry, Alexa Fluor® 594,PE-Cy5, PerCP, PE-AF680, PERCP Cy 5.5, Cy5, Alexa Fluor® 647, APC,PE-Cy7, Qdot-800, APC-Cy7

4 Analytes not limited to IFN-γ, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, p70, IL-13, IL-15, IL-17,IL-21, TNF-a, perforin and Granzyme B,total IgG, IgM, IgA, IgE, IgG1, IgG2, IgG4, IgG3 at single-cell resolution

5 Applications:

• Functional Status of individual cells- Antigen-specific cytokine responses,- Antibody specific secreting cells,- Cytotoxic T-cell activity,-• Detection of inflametory markers IL-4, IL-5, & IL-13

• Vaccine-induced antibody responses, (Efficacy)

• Antigen-specific memory B cells,

• Monitor tumor-infiltrating lymphocytes

6 Flash scan based features for the following in 6-12-24-48 and petri in addition to standard scan. To be used as an addon to the main unit and/or independent facility.

• Neutralization assays- High throughput colony counting & Viral titer- Plaque Reduction Neutralization Assay (PRNT)- Viral immune-colorimetric assay (ICA)- Focus forming assay

• Intracellular inclusion assay- Plaque / Focus-Forming Unit (PFU/FFU) assay

7 A patented deep machine-learning Al systems designed to offer robust solutions to the most difficult image analysis problems -detection of objects of interest for Live Cells Based Assays with Patent US 12,046,057 B1

8 Warranty: 5 years

9 Supply: 15 Days

Name of the firm-

Name of authroized signatory-

Indian Institute of Science Education and Research Bhopal

TECHNO-COMMERCIAL BID

Tender No.: IISERB/SP/2025-26/111 Dated 27.02.2025

Item - Supply & installation of Elispot Reader.

1 Name of Tendering Company with Registration No. & Date issued by appropriate authorities. (Please enclose copy of certificate of registration)

2 Do you possess trade license issued by Competent Authorities in India? If so, please enclose a copy.

3 Name of Proprietor / Director

4 Furnish following particulars of the Registered Office a. Complete Postal Address

b. Telephone No. / Mob. No.

d. E-Mail Address

5 Furnish following particulars of the Local Branch Office. (if any) a. Complete Postal Address

b. Telephone No. / Mob. No.

d. E-Mail Address

6 PAN No. (Attach Attested Copy)

7 GSTIN No. (Attach Attested Copy)

8 If Manufacturer – Pl. attach the certificate of Registration

9 If Authorised Dealer / Distributer – Pl. attach relevant certificate.

10 Financial turnover for the three financial Years. (Please attach copy of certificate by Chartered Accountant in original )

Financial Year Amount (In Lakhs ) Remarks, if any

(Attach separate sheet if space provided is insufficient)

11 Give details of the major clients – Educational Institutes/Universities, Government Departments, Research Organisations, to whom item/material of same type have been supplied by the bidder during the last five years in the following format.

Sl. No Name & address of the client with details - Name of the contact person, telephone no., Fax no., e-mail id Name & quantity of the items sold Purchase Order Work Order No. & date Purchase order / Work Order amount (Rs. In Lakh)

(If the space provided is insufficient, a separate sheet may be attached)

12 The agency should not have been black listed or banned by any Govt. Department, Government Organization, PSU, University, Autonomous Institute etc. A notarized certificate to this fact should be enclosed with techno-commercial bid as per Annexure-V.

13 Are you an ISO certified manufacturer? If so, please attach a copy of the certificate.

14 Please specify the minimum time required to supply the item / material from the date of receipt of the Purchase Order

15 Additional information, if any (Attach separate sheet, if required)

Name of Authorised Signatory:

Name of the Company:

Select, Excess (+), Less (-)

Tender Inviting Authority: Indian Institute of Science Education and Research Bhopal (IISERB)

Name of Item: Supply & installation of Elispot Reader.

Tender No: IISERB/SP/2025-26/111 dated 27.02.26

Name of the Bidder/ Bidding Firm / Company :

Sl. No. Item Description Item Code / Make Quantity Units Estimated Rate in Rs. P Addition / Deduction Addition / Deduction Values Currency Convertion against each Item Quoted Currency in INR / Other Currency BASIC RATE In Figures To be entered by the Bidder in Rs. P GST (If applicable in Percentage) GST Amount in Rs. P Discount offererd Any other charges Discounted price GST (If applicable in percentage) GST in Amount RS P HSN / SAC Code

1 Supply & installation of Elispot Reader. item3 LOT Excess(+) Full Conversion INR

Total in Figures

Quoted Rate in Figures Select %

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