Chemicals

PrimeScriptTM Ist strand cDNA Synthesis Kit Quantity: 1 Size: 50 rxns PrimeScript First Strand cDNA Synthesis Kit is designed for efficient synthesis of first-strand cDNA from RNA templates using PrimeScript reverse transcriptase. It provides high sensitivity and efficiency with a mix of oligo(dT) and random primers, enabling full-length cDNA synthesis. The enzyme functions optimally at 42–50 °C, producing high-quality cDNA suitable for RT-PCR and qPCR

RNAiso plus Size: 200ml RNAiso Plus is a monophasic solution of phenol and guanidine isothiocyanate used for isolation of total RNA from various biological samples. It enables efficient cell lysis and RNase inactivation, followed by phase separation to obtain high-quality RNA suitable for downstream applications such as RT-PCR, Northern blotting, and sequencing.

Mighty TA-cloning Size: 20 rxns Mighty TA Cloning Kit is designed for rapid cloning of PCR products with A-overhangs into T-vector systems. It allows efficient ligation without restriction digestion and supports quick transformation and screening of recombinant clones, making it suitable for routine cloning and sequencing applications.

Ni-NTA Agarose Size: 25 ml Ni-NTA Agarose is an affinity chromatography resin used for purification of His-tagged proteins. Nickel ions immobilized on nitrilotriacetic acid (NTA) bind specifically to histidine residues, allowing selective purification under native or denaturing conditions. Elution is typically achieved using imidazole.

QIAprep Spin Miniprep Kit Size: 250 prep QIAprep Spin Miniprep Kit is a silica membrane-based kit for rapid isolation of plasmid DNA from bacterial cultures. It uses alkaline lysis followed by selective DNA binding and washing steps to yield high-purity plasmid DNA suitable for sequencing, cloning, and restriction digestion. The procedure is fast and typically completed within 20–30 minutes.