SITC of High Performance Liquid Chromatography

PUNJAB BIOTECHNOLOGY INCUBATOR

COMMERCIAL BID FORMAT - HPLC with PDA, RID & FLD

Name of the Manufacturer

Make of the Equipment

Country of Origin

Name of the Bidder

Part A - Main Equipment Qty Rate in Rs. GST% Amount in Rs.

For Honey application as per National/International requirements 1 set

High pressure Liquid Chromatography (HPLC) equipped with Quaternary gradient solvent delivery system with capability of operating detectors. The system should be quoted with all accessories required to make it fully operational and any other item required for stated applications be quoted as optional.

Technical Specification:

Quaternary Gradient with Online Degasser.

Pressure operating range 7000 psi or better

Flow Rate Range: Programmable 0.01 to 5 ml/ min in 0.01 ml/min increments

Flow Precision ±0.1% RSD or below

Flow Rate Accuracy ±1%

Eluent Degassing Online Degasser for all channels

Gradient Mixer Quaternary mixing & gradient capability using proportionate valve

Solvent Setting Range 4 solvents setting range:0-100% with 0.1% step

Diagnostic Features Error detection and display, Leak detection & safe leak handling

Wavelength range 190-700 nm

Linearity range Linearity range <5%

Baseline noise 3.0 X 10-5 AU at 254 nm or better

Drift <1.0x 10-3 AU/h at 254 nm

Suitable peak purity software, Auto threshold for peak purity

Refractive Index Range 1.00 to 1.75 RIU

Noise Level ± 2.5X10-9 RIU

Drift Drift 2x10-7 RIU/hr

Cell Volume Approximately 10 μl or better

Temperature Control Temp controlled Flow cell unit

Light Source Xe lamp with standard wattage

Wavelength Range 200-600nm (Excitation)

Wavelength Accuracy ±3nm

Wavelength Reproducibility/ Repeatability ±0.25nm

Sensitivity S/N Raman peak of water ≥ 1000 or S/N >550 ASTM

Flow Cell volume approximately 8 μl or more

Wavelength Scanning Scanning of Excitation and Emission wavelengths

Pressure 1 Mpa or better

5 COLUMN COMPARTMENT

Temp. range Ambient to 80° C or better

For column length 300 mm

No. of Columns accommodated Minimum 2

Temperature Stability ±0.3 ºC of set temperature

Injection Volume Range 0.1-100μl (Standard)

Sample Capacity >80 x 1.5/2 ml vials or more

Injection Volume Accuracy ±1%

Injection Precision <0.5% RSD or better

Carry over 0.005% from previous injection

Tray Temperature Operating Range 4 - 40°C programmable in 1°C increments

7 SOFTWARE AND HARDWARE

Software: Software with multitasking and capable of performing the following functions: Control the system, acquire, store, process and reproduce the data. It must be able to control all the devices from same software Factory certified and/or Branded Computer System with i7/ Xenon or better processor, 32 GB RAM or better and 2TB HDD or better, 22-23” or larger Full HD Monitor with licensed latest windows compatible with equipment Software, MS office Latest standard version and Antivirus for 3 year. System should have minimum 2 network ports. Printer – Laser B/W - duplex - Legal, A4 with Network Card and Bluetooth/Wifi facility. 1 set

8 DOCUMENTS AND TRAININGS

On site IQ, OQ of instrument along with document

Training Satisfactory technical and application training to the personnel at site immediately after installation.

Total Amount of Part A

PART B - Other Requisites for HPLC

HPLC COLUMNS C18=300 x 4 (id) mm Carbohydrate or equivalent - 2 no./Set 1 Set

C8 = 150 X 4.6 X 5μm - 2 no./Set 1 Set

Cyano = 150 X 4.6 X 5μm 1 no. 1 No.

Amino = 250 X 4.6 X 5μm - 2 no./Set 1 Set

Phenyl = 250 X 4.6 X 5μm 1 no. 1 No.

Silica = 250 X 4.6 X 5μm 1 no. 1 Set

· Fittings, Frits, ferules and Tubing’s 1 Set

· Tubing cutter - 02 no./Set 1 Set

· Solvent bottles 1000ml capacity - 04 No./Set 1 Set

· Solvent filters (Glass & SS both, 04 no. each) 1 Set

Total Amount of Part B

PART C - Extended Warranty Charges (After expiry of Standard Warranty of One (01) Year

1st Year Extended Warranty Charges

2nd Year Extended Warranty Charges

3rd Year Extended Warranty Charges

4th Year Extended Warranty Charges

5th Year Extended Warranty Charges

6th Year Extended Warranty Charges

Total Amount of Part C

Total FOR Amount upto PBTI Site - Part A + Part B + Part C

Total Amount in Words:

ü It is mandatory to quote item wise rates for Part B & Part C, lump sum rates are not acceptable for these.

ü Bidder must quote amount in the given cells, if amount against any cell is included in main component, bidder should put "Zero" value.

ü Taxes must be mentioned, if it is not mentioned, the same shall be treated as included in given rates.

Signatory Name:

HPLC - List of any other items like consumables required for trouble free operation of equipment for 06 years after the expiry of guarantee/warranty period which are not covered in CMC

Sr. No. Name Qty. Rate

Remarks, if any:-

Name of Company

Name of Authorzed Signatory