Chemicals/reagents required to the VGST-CISEE sponsored project Dept. of Applied Zoology, Mangalore University, Mangalagangothri-574199

Chemical name CATALOGUE NO QUANTITY Make

Oral gavage feeding canula ( 16 No) 304 8 vishnu enterprise /any make

0.2µm syringe filter SF0-100NO 2 packet Himedia

PBS (pH 7.4) SFGF513B 4 PACKET Himedia

methanol 34860-2.5L-R 2 bottle Merck

Glacial acetic acid 695092-500ML 2 bottle Merck

Tunicamycin T7765-5MG 2 Sigma-Aldrich

NaCl GRM853 2 bottle of 500 g HiMedia

Qubit protein Assay (12.5μg/mL to 5mg/mL) Q33212 500 reactions Thermo Fisher

Trizol 12183555 2 bottle of 100 ml ThermoFisher

Chlorofom AS114 2 bottle of 1L HiMedia

Phenol MB082 2 bottle of 100 ml HiMedia

Alcohol molecular grade 5 bottle -500mL Himedia

Isoproponol 423835000 2 bottle of500 ml ThermoFisher

Sodium citrate TC526U 2 bottle of 100 g HiMedia

DEPC water TCL018 2 bottle of500 ml HiMedia

Tris buffer TC072 2 bottle of 100 g HiMedia

EDTA 798681-100G 2 bottle of 100 g Sigma-Aldrich

QUBIT RNA BR ASSAY KIT, 500 Q10211 1 Invitrogen/thermo

cDNA kit PrimeScript™ RT Master Mix (Perfect Real Time) RR036A 1 TAKARA

RNAse free water 9012 10 bottle of 10 ml takara

qPCR master mix (Takara) A25741 4 ml Thermofisher

caspase-12 Antibody (1611) sc-21747 2 x200 µg/ml santacruz

USAG1/SOSTDC1 Rabbit anti-Human, Mouse, Rat, Polyclonal, NB093957 2x100 µl Novus Biologicals™

WFS1 Antibody #8749 8749S 2x100 µl cell signalling

PrimePCR™ SYBR® Green Assay: DDIT3, Rat 639676 2x200 Rxns TAKARA

Strep-HRP antibody sc-69710 2x500 µl supernatant santacruz

Streptavidin-HRP 3999S 2X1 ml Cell Signalling

GRP78 / BiP sc-13539 2x200 µg/ml santacruz

Beclin-1 (BECN1/Beclin-1 Antibody (E-8) sc-48381 2x200 µl santacruz

LC3-II (specific) LC3B Antibody ABC99 100 µl Merck

LC3 2775S 100 µl Cell Signalling

mTOR 2972S 100µlL cell signalling

anti atg3 3415T 20µl cell Signalling

SQSTM1/p62 Antibody 5114T 20 µl CELL Signalling

Atg7 Antibody 2631T 20 µl cell Signalling

step™ NBT/BCIP Substrate Solution 34042 250 Ml thermoFisher

Atg12 (D88H11) Rabbit Monoclonal Antibody 4180T 20 µl cell Signalling

anti-CHOP SAB4500631-100UG 2x100 μg santacruz

secondary antiboy- goat anti-rabbit IgG-HRP 65-6120 2X1ml thermofisher

skimmed milk powder LP0033B 2x500g ThermoFisher

TBST TBST-500/TBST-500-1 2x500ml Biologix®

Western BLoT Quant HRP Substrate T7102B 200 mL takara

Western BLoT Blocking Buffer (Protein Free) T7132A 500 mL takara

Acrylamide/Bis-acrylamide solution A3699- 100ML MERCK

TEMED T7024- 100ML MERCK

SDS Sodium dodecyl sulfate L3771-25G 25G MERCK

AMMONIUM PERSULPHATE A3678-25G 25 g MERCK

GLYCEROL G5516 100ML Merck

TWEEN® 20 P7949 100ML MERCK

GLYCINE Glycine (White Crystals or Crystalline Powder), Fisher BioReagents™ BP381 500 GM FISHER

Coomassie Brilliant blue R 250 (C.I. 42660) 1125530025 25 G MERCK

Methanol, Gradient grade AS128 2500 ML HIMEDIA