RPF of Supply of Confocal Microscope

Amount

REQUEST FOR PROPOSAL Supply of Confocal Microscope.

REQUEST FOR PROPOSAL Supply of Confocal Microscope Fully integrated and enclosed Confocal based system should be capable of highest possible detection efficiency, Instant highly resolved Realtime SR imaging modality to have resolution 120-140 nm in XY and 300-400 nm resolution in Z. Multimodal imaging capabilities such as simultaneous multichannel fluorescence imaging, Z-stack, co-localization, time-lapse imaging, Advanced 3D imaging etc. should be available as standard; Complete automated operation for sample loading, detection and calibration; Optical requirements: Dedicated 850 nm LED based focus drift compensator for maintaining focus continuously during long term live cell imaging, anti vibration table, Objectives: Plan Fluotar 1.6x with NA 0.05 or higher, Plan Fluotar 10x with NA 0.32 or higher for fluorescence, DIC/IMC/plas DIC, Plan Apochromat 20x with NA 0.75 or higher for fluorescence, DIC/IMC/plas DIC, Plan Apochromat 40x with NA 1.30 or higher oil immersion for fluorescence DIC/IMC/plas DIC, Plan Apochromat 63x water immersion with NA 1.20 or higher for fluorescence, DIC/IMC/plas DIC; Illumination: White LED for transmitted light applications for morphology imaging with pseudo 3D appearance applicable for glass and plastic vessels, At-least four solid state LEDs of 365nm, 470 nm, 560 nm, and 625 nm for Fluorescence applications with real time triggering capability, Minimum 4 individual laser lines 405nm, 488nm, 561nm and 638nm for confocal and Super Resolution imaging. Direct modulation for all laser lines, global neutral density filters to automatically adjust dynamic range to sample in automatic illumination control; Stage: Motorized xy scanning stage controlled by system software and dedicated controller/joystick, Travel range of minimum 127 x 83 mm or higher, XY resolution of 0.02 um or lesser, Reproducibility of <1 um or lesser, Fast scanning speed of 37 mm/sec or higher, Should include sample holders for 4 slides, 35 mm petri dish and 60 mm petri dish; Detection systems: Minimum 4 dedicated highly sensitive monochrome CMOS detectors with minimum 5 MP or more resolution for 4 color simultaneous imaging in WF mode, At least 1 camera/point detectors for morphology imaging in DIC/IMC/Phase contrast modes/H&E staining or similar applications, The system must be equipped with Point Scanning/Spinning Disk based confocal scanning techniques, Minimum 4 or more multi-pixel HyD/GaAsP point detectors for 4 color simultaneous confocal imaging with minimum 40% or higher PDE, Advanced real-time controller for high-speed synchronization of system components (stage, illumination, focus, detectors) allowing high speed imaging and minimizing light exposure, photodamage and bleaching, The system must be equipped with integrated online and instant Super resolution imaging modality with resolution 120-140 nm in XY and 300 to 400 nm resolution in Z; Incubation system: Should offer climate control of the complete sample space. Should offer software control of all parameters including CO2, temperature, and humidity without any manual intervention. Incubation system should offer 100% CO2 input with software-controlled CO2 mixer; Software for Image analysis, 3D reconstruction & processing, Multi-position and Time-lapse option, Colocalization; Workstation: High performance factory integrated workstation PC with Windows 10 to T enterprise or higher OS, Intel Xeon or higher processor, minimum 192 GB or higher DDR4 RAM, NVIDIA Quadro RTX 6000 with minimum 24 GB or higher RAM, 8 TB or higher SSD, DVD RW and 30 inches monitor; Complete in all respect for Installation and Operation. or Advanced version. With UPS system as mentioned in special terms and conditions.