SUPPLY OF CHEMICALS, REAGENTS, CONSUMABLES, GLASSWARE PLASTICS MISCELLANEOUS ITEMS Common Items

Consumables for different departments

0.4% Toluidine blue solution,500 ml

2-mercaptoethanol,

4'6'-diamidino phenylindole (DAPI)

Acute Peritoneal Dialysis Fluid -1Litre bag

Agarose (low EEO) (Sigma) 25 gm

Alcohol,Ethyl alcohol Histopathology grade 100 Percentage 5L

Amido black,500gm

Ammonium acetate

Ammonium bicarbonate ( Molecular Grade )

Ammonium chloride ( Molecular Grade )

Basic Fuchsin pkt. Of 25 gm

Benzidine ,250 ml

Bile Salts 500,500 g

Bleaching powder,500gm

Bouin’s fluid,4L

Brilliant Cresyl Blue for reticulocyte count (125ml),For reticulocyte count (125 ml)

Chlorhexidine scrub bottle (500 ml)

Chloroform,Purity>/=99.5% molecular grade for nucleic acid extraction, 500ml

Clove oil 100 ml,

Deoxyribonuclease

DiEthyl PyroCarbonate (Sigma/Himedia) (Molecular Grade)

Disodium salt of ethylene diamine tetraacetic acid (EDTA) ( Molecular Grade )

DPX bottle,500 ml, Sigma Aldrich/ Merck

DTT (Dithiothreitol),50gm

Ethanol,Molecular grade, 100%, 200 proof, 500ml/bottle

Ethidium bromide ,10ml

Ethyl alcohol (Mol grade) 100% (Fermentas,Sigma-Aldrich,MP diagnostics,Invitrogen)- pack of 500 ml.

EUSOL (400ML)

Giemsa stain,100 ml

Glutaraldehyde 2% 5 litre Can,

Glutaraldehyde 25 Percentage,100 ml

Glutaraldehyde solution 2.4%. Activated 2 Liters can

Glycerol 99.5% AR, 500ml

Glycine powder,500 gm

Guanidine thiocyanate

Hexane ( Molecular Grade )

Histopaque (1.077)

Hydrochloric acid Concentrated( Molecular Grade )

Iron hematoxylin stain,1 kg

Isoamyl alcohol

Isopropanol( Molecular Grade )

Lugol's iodine, 125ml/bottle,125 ml

Magnesium Chloride ( Molecular Grade )

Methanol,AR grade 2.5 lit /bottle

Methyl Red Indicator,125 ml

Methylated - Control DNA Pack(Invitrogen/ Qiagen/ Zymo/ Takara)

Millipore grade ultrapure RNA/DNA/nuclease free water

miRNA isolation kit from plasma/Serum

N/10 HCL,500ml

Neutral red powder ( 0.1%),25 gm

New Methylene blue ,25 gm, AR

New Methylene blue,250 ml, AR

Normal melting Agarose (Sigma/Himedia/SRL)

Orcein, powder, 10 gm

Paraffin oil light,500 ml

PBS Balanced

PCR- grade water (Sigma/Himedia)(Molecular Grade)

Peppermint Oil,For test of smell sensation (100 ml)

Peptone, Bacteriological,(Difco, Himedia, Oxoid, Merck, Sigma Aldrich)500 g

Phenol ( Molecular Grade )

Phenol equilibrated with Tris &EDTA pH-7.8 (Sigma)

Phosphate Buffered saline MB grade (Himedia/Sigma/Amresco/Biobasic/Lonza/Promega/mp bio)

Polycation polyethylenimine (PEI)

Potassium Chloride ( Molecular Grade )

Potassium dihydrogen phosphate,AR range 500gm/pack, purity >/= 99.9%

Povidone Iodine solution 10% 500 ml bottle

Primers for gene expression analysis (IDT)

Primers for methylation analysis (IDT)

Proteinase K (Qiagen/Sigma/ Invitrogen)

Ready to use Gram Stain Kit,1 kit containing 125 ml each of crystal violet, Gram's iodine, acetone and safranin

Ready to use India ink Stain ,23 ml

Ready to use Ziehl Neelsen stain kit,1 kit containing 125 ml each of Carbol fuschin, acid alcohol and methyl blue

Ready-to-use India ink stain,23 ml

Real time PCR Taqman assay by custom design for Gene Expression

Real time PCR Taqman assay by custom design for Gene Expression for BETA-ACTIN

RNase Zap RNase Decontamination solution

SDS(sodium dodecyl sulphate),Molecular grade,free from DNAse/RNAse, 100gm

Silver nitrate, 0.2 Percentage, 250 ml

Sodium acetate ( Molecular Grade ), 250gm

Sodium Chloride ( Molecular Grade )

Sodium chloride (NaCl) (0.9%)Solution,0.009

Sodium Citrate ( Molecular Grade )

Sodium Citrate,pH 7.5-9.0, heavy metals ≤5ppm

Sodium Deoxycholate powder for bile solubility,100 g

Sodium dodecylsuphate (SDS-PAGE/PCR-RFLP)

Sodium hydroxide pellets ( Molecular Grade )

Sodium hypobromide for synthesis,500 ml

Sodium pyruvate,25gm

Sodium thiosulphate, 5 Percentage, 500 gm

Spirit (Methanol LR For synthesis),1 lit bottle

Spirit/IPA 70%,100 L , IPA 70%

Sterile saline 0.9%,50x10 ml pack

Sucrose dehydrated extra pure,500 g

Surgical Scrub containing Povidone iodine 7.5% w/v. 500ml with pump. Should have high foaming and viscosity ability.

Tris (Merck/Himedia/Loba/SRL/Sigma)(pure powder)

Tris base,500gm

Tris HCL,500 gm

Tris-HCl ( Molecular Grade )

Triton-X ( Molecular Grade ), 100 ml

Trizol ( Molecular Grade )

Trypsin

Trypsin,1000mg

Tween 20

Tween 80

Bone cutter electric,Steel body

Bone wax 2.5g packs/foil

RPMI-1640 Medium with L- glutamine and without sodium bicarbonate. Powder form and should make 10 L.

Slide storage Cabinet,3 Feet Box, storage for 3000 slides

Cigarette Lighter,Made of transparent plastic material, LPG based, 5/pack

Beaker 50 ml ( glass

Candle jar,500 ml capacity

Centrifuge tubes 50ml

Cryoboxes ,Polypropylene,Box with attached lid

Dispensing Bottles ,dropping bottle, 60 ml, plastic, nozzle

Glass dropper (20 ml),20 ml, with Rubber teat

Glass narrow mouth stoppered bottle 250 ml

Glass narrow mouth stoppered bottle amber colored 125 ml

Glass narrow mouth stoppered bottle amber colored 50 ml

Glass stirring rod,Flat face paddle.dimension = 250mm X 6mm

Measuring cylinder 25ml

Pasteur pipette ,Plastic, borosil

Plastic can(5 liter),5 liter capacity

Plastic dispenser for acid with grauated volume(Range- 1ml to 10ml)

Sample Transport boxes

Small glass bottle with dropper (30 ml),

Test tubes 2ml

Test tubes with rim 15x100mm

High resolution DSLR,1.Batteries: 1 Lithium-ion battery 2.Memory Storage Capacity:16 GB 3.Included Memory Card Size: 16 GB 4. Display Technology: Fully LCD 5.Standing screen display size:3 Inches 6.Optical Zoom:7.5 7.Optical Sensor Resolution: 24.1 MP 8.Min Focal Length: 18 Millimeters 9.Video Capture Resolution: 4K

Steel forceps Small, Stainless steel, blunt tip

Hemoglobin tube for sahli’s hemometer with rubber tube and mouth piece,For round comparator box Hb tube should be having round bottom

Autoclavable Micro pipette filter tips with aerosol barrier 0.1-2.0 µl (Sterile sealed)-ultralow retention tips

Autoclavable Micro pipette filter tips with aerosol barrier 0.2-10 µl

Autoclavable Micro pipette filter tips with aerosol barrier 10-1000 µl

Autoclavable Micro pipette filter tips with aerosol barrier 2.0-20 µl

Autoclavable Micro pipette filter tips with aerosol barrier 200-1000 µl

Autoclavable Micro pipette filter tips with aerosol barrier 20-200 µl (Sterile sealed)-ultralow retention tips

Butter paper,Non-absorbent

Centrifuge tubes (0.2 ml)

Centrifuge tubes (0.5 ml)

Centrifuge tubes (1.5 ml)

Centrifuge tubes (2.0 ml)

Cryo Vial 1ml

Filter paper 41x41cm(Ordinary)

Filter tips 0.5-10ul

Filter tips 100-1000 ul

Filter tips 10-100ul

Glass marker diamond tipped pencil,With wooden handle

Glass marking pencil,White

Hemoglobin pipette with rubber tube & mouth piece,1.5 ml

Homogenization tube for 5ml

Kinyoun stain kit,Kit includes one 250ml . bottle of each, carbol fuchsin (kinyoun), acid alcohol (decolorizer) and methylene blue (counterstain) in a kit

L mould ,For making blocks

Laboratory Cover slips For hemocytometer,For hemocytometer, size = 22x25x0.4mm

Laboratory Cover slips,22x22mm, 22x40 mm, 22x50mm,

Membrane filter 0.22μm

Membrane filter 0.45μm

Microcentrifuge tubes 0.5 ml

Microcentrifuge tubes 2 ml,

Microcentrifuge tubes ,1ml

Pepsin(bovine),5gm

Petri dish 50X17 mm

Plates for PCR

Polymerase Chain reaction strips with transparent caps compatible to BIORAD CFX-96

Polymerase Chain reaction tube (200 ul)

RBC pipette with rubber tube & mouth piece,1.5 ml

Screw vial closure 2.0ml

Spin column for RNA isolation (Qiagen/Invitrogen/Takara/MN)

Storage Vial PP'/HDPE 2ml autoclavable

Test tube 12 x 100 mm glass

Thin walled 0.2 ml PCR Tubes Polypropylene (colourless), strips of 8

Thin walled 0.5 ml PCR Tubes Polypropylene(colourless) and separate Caps, strips of 8

Washing Gloves,1 pair, vinyl rubber gloves, free size

WBC pipette with rubber tube & mouth piece,1.5 ml

Brushes for washing test tubes

Rubber bulb for pipettes large

Small Brushes for washingtest tubes,Small size,

Stainless steel forceps

Ziplock bag (Freezable-polypropylene) -Large

Absorbable Tacker 5mm For Mesh Fixation With 30 Tacks.USFDA/European CE Certified

Dissection board,

Pipette stand for autopipettes,Stand for 5-6 pipettes

Slide storage cabinets,20000 slide storage in 1 cabinet

Spirit lamps,63x63 mm (HxD), 125 ml, stainless steel with cap

Assorted Nichrome loop,

Autoclavable adjustable research pipette 0.2-2.5 µl

Autoclavable adjustable research pipette 1-10 µl

Autoclavable adjustable research pipette 20-200 µl

Autoclavable adjustable research pipette 2-20 µl

Autoclavable polypropylene Rack with lid for 0.2 µl PCR tube strips

Autoclavable polypropylene Rack with lid for 0.5 µl PCR tube strips

Autoclavable polypropylene Rack without lid for 0.2 µl PCR tube strips

Autoclavable polypropylene Rack without lid for 0.5 µl PCR tube strips

Centrifuge Tube Stand 15ml (12x3 holes, suitable for -80 degrees storage)

Cryoboxes,Polypropylene, Box with attached lid,

Glass Staining racks/troughs ,

Pipette stand (for holding 4/6 micro pipette )

Plastic microtip box 0.2-10 μl,0.2-10 μl,

Plastic microtip box 200-1000 μl

Plastic microtip box 20-200 μl

Rack ( 1 way) to hold 15ml conical centrifuge tubes 12 places

Rack (3-way) to hold 0.2 ml, 0.5ml and 1.5 ml micro-centrifuge simultaneously

Rack (4 way flipper) to hold 15 ml, 50ml, 1.5 ml, 0.5 ml tubes.

Rack reversible for microtube (0.5'/1.5 ml) PP 96 places

Slide Tray ( stainless steet / PVC Tray),Stainless steel tray / PVC tray, holds 30 slides/wet mounts flat

Stainless steel forceps Size 6

Steel forceps Medium

Storage racks for specimen,surafce colour coated Mild steel height 6-8 feet number of layers 4-5

Test tube basket for 15cm test tubes (Stainless steel )

Test tube holder,Stainless steel with adjustable handle

Tripod stand with mesh,Made up of non-corrosive material

Wiremesh(8'×8') iron/copper

2,6 dichloro phenol indophenol, 100gm

Buccal mucosa DNA extraction kit

DNA extraction kit for human blood PBMCs (Qiagen/Invitrogen/Roche/Takara)

DNA extraction kit for human whole blood (Qiagen/Invitrogen/Roche/Takara)

DNA isolation Kit

DNA ladder 1 Kilobase pair (NEB/Fermentas/kapa)

DNA ladder 100 base pair (NEB/Fermentas/kapa)

DNA ladder 50 base pair (NEB/Fermentas/kapa)

DNA Loading Dye 6x (NEB/Fermentas/kapa)

EDTA 250 gm,Disodium EDTA, molecular grade,

Human Alpha Smooth Muscle Actin ELISA kit

Human Beta 2 microglobulin ELISA kit

Human CXCL10 ELISA kit

Human CXCL-10 ELISA kit

Human CXCL-10 ELISA kit

Human CXCR3 Human ELISA kit

Human E-cadherin

Human Fibroblast growth factor 23 ELISA kit

Human High capacity cDNA conversion kit with reverse transcriptase and buffer (with RNAse inhibitor) for blood-derived PBMCs (Qiagen/Invitrogen/Roche/Takara)

Human IL – 1 alpha ELISA kit

Human IL – 1 Beta ELISA kit

Human IL - 10 ELISA kit

Human IL - 12 ELISA kit

Human IL - 13 ELISA kit

Human IL - 17 ELISA kit

Human IL – 2 ELISA kit

Human IL - 21 ELISA kit

Human IL - 22 ELISA kit

Human IL - 23 ELISA kit

Human IL - 28A ELISA kit

Human IL - 29 ELISA kit

Human IL - 4 ELISA kit

Human IL - 6 ELISA kit

Human IL - 9 ELISA kit

Human IL 18 ELISA kit

Human IL-6

Human IL-6 ELISA kit

Human Interferon γ ELISA kit

Human interleukin1-beta

Human pten (IL-1 Beta) ELISA kit

Human serum Interleukin-10

Human serum Interleukin-17

Human serum Interleukin-23

Human Soluble Epithelial Cell Adhesion Molecule (sEpCAM) ELISA kit

Human soluble Intercellular adhesion molecule (ICAM-1)

Human TNF-α ELISA kit

Human Vascular cell adhesion molecule (sVCAM)

Human VEGF ELISA kit

Iodine testing kit,

ladder,100bp DNA Ladder M, 200 lane(Fermentas,Sigma-Aldrich,Bangalore Genei (Merck),Invitrogen)-

masson trichome technique (acid fuschin), 250 ml, Srlchem, pubchem,sigma, merck, aldrich, fischer scientific

Methylation specific PCR Mastermix(Invitrogen/ Qiagen/ Zymo/ Takara)

miRNA isolation kit from tissue

mi-RNA sequencing: Reads required are 30 Million reads and Pathway analysis for target prediction of novel miRNA

Modified trichrome stain,5 G

Nuclease-free water (PCR grade) (Sterile)

Octanol( Molecular Grade )

Plasmid DNA kit

Primary Antibody (House Keeping)

Primary Antibody (Target)

Protein Ladder (Broad range multicolour)

Protein sequencing and proteome analysis

Ready-to-use Ziehl Neelsen stain kit,1 kit containing 125 ml each of Carbol fuschin, acid alcohol and methyl blue (Merck)

RNA extraction kit from tissue

RNA Isolation Kit

RNA Isolation kit for human blood PBMCs (Qiagen/Invitrogen/Roche/Takara/MN)

RNA Sequencing

Secondary Antibody (Mouse/Rabbit) depends upon primary Ab origin

SYBR green master mix for Real Time PCR compatible with CFX 96 Biorad (Roche/Invitrogen/Qiagen/Takara)

Taqman assay by design for quantitative PCR for miRNA assay (Invitrogen/Qiagen)

Taqman Genotyping master mix for Real Time PCR

Taqman Universal Master mix for miRNA

Transfection reagent

Unmethylated - Control DNA Pack(Invitrogen/ Qiagen/ Zymo/ Takara)

Whole exome sequencing (outsourcing)

Acute peritoneal dialysis fluid infusion & drainage set

Capillary tubes for clotting time ,For capillary method of measurement of clotting time

Cotton mops with xray detectable thread for OT

disposable c arm cover

dynaplast bandage 6 inch

Elbow length long glove 7 No. (box of 50) powderless

Electrode with PTFE for cautery pencil Bayonet Blade Electrode Modified, USFDA/ European CE

External Defibrillator Pads, Adult

External Defibrillator Pads, Pediatric

Fogger solution 1 liter

Gigli saw wire.

Glass Marking pencil (white),White

IOBAN sterile drape 60 X 45 cm

IV extension line 10 cm

K-90 Catheter

Nebulization kit (Face mask + nebulization chamber) Adult

NIV Oronasal Mask with straps. Disposable. Size L. Should have soft silicone sealing interface to the patient's face with anti-asphyxia valve and exhalation port

NIV Oronasal Mask with straps. Disposable. Size M. Should have soft silicone sealing interface to the patient's face with anti-asphyxia valve and exhalation port

NIV Oronasal Mask with straps. Disposable. Size S. Should have soft silicone sealing interface to the patient's face with anti-asphyxia valve and exhalation port

NIV Oronasal Mask with straps. Disposable. Size XL. Should have soft silicone sealing interface to the patient's face with anti-asphyxia valve and exhalation port

Quincke's spinal needle 25 G

Spacers Device for Inhaler

Spongstone/ Gelfoam

Teasing needles,

Tegaderm with pad medium 9 cm x 15 cm

Whatmann filter paper grade I,125 mm

Prolene 1 cutting

SHIV DIAL SUD AND SONS

Microlit

Kalpatru Scientific

Deep Distributors

Alfa Instruments

International Scientific and Surgicals