Supply and installation Confocal Raman Spectrometer and 4.2K closed cycle optical cryostat

Indian Institute of Science Education and Research Bhopal

Annexure-I(b) Annexure-I(b)

Technical Compliance Sheet

Tender No.: IISERB/SP/2025-26/104 Dated 17.02.2026

Item: Supply and installation Confocal Raman Spectrometer and 4.2K closed cycle optical cryostat.

Sl. No. Specifications Make and offered Model Authorization certificate attached/Not attached Comply / Not Comply

Confocal Raman Spectrometer:

· Confocal Raman spectrometer with the capabilities of recording Raman spectra and Photoluminescence spectra. The Spectrometer should be inclusive of research grade microscope/spectrometer with objectives, detectors, Lasers, optics and along with computers & software with the following detailed specifications.

· High throughput Lens Based spectrometer with a focal length minimum of 600mm or higher.

· Overall throughput of the lens-based spectrometer should be more than 65%

· Output coupler for microscopes with exit port for spectrometer connection including optical fiber.

· Core diameter of the Optical fiber should act as confocal detection unit for the coupling of the collected Raman, fluorescence, or reflection signal

· A Multiwavelength laser coupler should be able to adapt up to three excitation sources. It should allow comfortable switching between the excitation wavelengths including automatic alignment of all necessary optical components without any manual intervention in the optical beam path.

· It should include Housing and mechanical parts.

· Filter positions unloaded to add any desired filters set.

· Scan to Scan repeatability should be 0.04 cm-1 or better

· Spectral resolution: 0.5 cm-1

· Motorized software controlled six grating turret with minimum of Three gratings mounted and interchangeable.

· The spectrograph should allow spectral coverage from 80 cm-1 to 4000 cm-1 in one single continuous acquisition without any step and stitches maintaining 1cm-1 spectral resolution or better

· System should have a coupler set to be compatible to accommodate 3 wavelength excitations or more

· All the lasers should be air cooled for maximal confocal performance and should be highly efficient single mode Photonic Fibers which are coupled to the microscope/spectrometer.

· All excitation sources are connected to the microscope via FC/APC single-mode optical fibers to guarantee diffraction limited point-illumination for highest possible confocal resolution.

· The filter set for each wavelength including laser line filter and edge filter, should be provided.

· Laser power intensity should be continuously variable by an attenuator controlled from 0 to 100% maximum with a minimum step size of 0.1%.

· Air-cooled, Solid-state Diode Laser @ 532nm with 70mW power or more should include optical isolator and fiber coupling unit directly connected to microscope/spectrometer system. Required ultra-low cut off filter set allowing Stokes-and Anti-Stokes Raman measurements starting at approx. 10 rel. cm-1

· Air-cooled, Solid-state Diode Laser @ 633nm with 30mW power or more, should include optical isolator and fiber coupling unit directly connected to Microscope/spectrometer system. Required cut off filter set Raman measurements starting at approx. 80 rel. cm-1

· Air-cooled, Diode Laser @ 785nm with 120mW power or more, should include optical isolator and fiber coupling unit directly connected to microscope/spectrometer system. Required cut off filter set Raman measurements starting at approx. 65 rel. cm-1

· A suitable Optical power meter head to be provided with a console readout unit through USB Interface and software.

· A multichannel high-performance CCD with the following specifications to be quoted.

· Back-Illuminated CCD chip with 2000 x 256-pixel format or better

· Peltier cooled to -55°C or better.

· Pixel Size: 15 µm x 15 µm or lower

· Optimized QEmax > 95%

· Low noise 16bit A/D converter

· USB interface and software controlled.

· A high stability research grade microscope/spectrometer for allowing confocal measurements with ≤ 950nm depth resolution and spatial resolution ≤ 300 nm by using a 100x objective

· Motorized z-stage system for automated approach, 30mm travel range with single step size of 10 nm resolution should be an integrated part of the microscope/ spectrometer with Autofocus features.

· Sample size of upto 100 mm dia or more should be accommodated and thickness of 20 mm or more

· The microscope/spectrometer should be branded research grade with 6x objective turret or more, colour camera for viewing and LED white light source.

· The Upright Microscope should have an optical port to connect fibers for individual wavelengths between laser and microscope.

· Standard Objectives with 10x, 50x, 100x magnifications to be included.

· Long working distance objective 20 x / 50 x suitable for use with low temperature stage

· The microscope should have compatibility of DIC, Dark-field, Fluorescence Imaging on upgrades.

Motorized X-Y-sample scanning stage for microscope/spectrometer should be fully integrated with Control Interface.

· 25 x 25 mm2 travel range or more

· Default step size: 25nm.

· Reproducibility better than <0.01%

· Software controlled

Advanced digital controller and Computer System

· Advanced digital controller based on a System-on-a-Chip concept. The digital component of the system should be based on one Field Programmable Gate Array (FPGA). This type of architecture results in speed, flexibility, accuracy, expandability, and precise timing, while simplifying operation and extensions from a single hardware device should enable all modes of operation.

Integrated Computer System for control and data acquisition.

· Intel i5 CPU or higher versions

· Minimum of 16GB RAM

· 500 GB SSD + 1000GB HD

· Keyboard and mouse

· OS: Windows 10 /11 64 bit

27-inch or larger Monitor

Control Software and Advanced Data analysis software

· Control and data processing software instrument control and data acquisition software, fully integrated data analysis and presentation software with image capture software for white light image display and capture. The state-of-the-art control system should be compatible with and optimized for the application (control, acquisition, and analysis) software to automatically perform the various measurement options.

To include an Add-on software package for advanced Raman imaging and data evaluation with following functionalities: -

· Automatic determination of Components based on residual analysis.

· Real-time image refresh for visualization of refined results

· Intuitive Demixing of components by weighted subtraction algorithms

· Multivariate Data analysis: Cluster analysis, Principal component analysis (PCA) & Non-negative matrix factorization (NMF)

· Advanced fitting tool: Fitting of full 2D/3D datasets.

· Data cropping and reduction

· Image correlation & Overlay

· Various filters e.g., Fourier, Anisotropic, Edge, Sharpen, User-Customized filters and many more.

· Graph and image repair

4.2k closed cycle optical cryostat

1. Completely cryogen free closed cycle cryostat for operation between 4.2K and 300K (at sample stage) for sample in vacuum configuration. This specification to be demonstrated for site acceptance.

2. Cooling capacity at sample stage should be at least [email protected]. The cryocooling system should be modular and be able to cool multiple cryostat (optical, microscopy, magneto-optical) modules separately. System should be supplied along with all accessories needed for running cryostat

3. Cryocooler should be outside optical table for better vibration isolation from experiment and support orientation free mounting. The vibration level should be less than 30 nm at the sample stage. To be substantiated with data.

4. No requirement of Helium gas for every cooling/heating cycle.

5. Modular microscopy module working distance of < 6mm between sample and objective in reflection mode.

6. One top window made of Quartz with >1-inch clear aperture. Transmission of windows should be 300-2000 nm. User changeable windows. Vacuum port for evacuating the sample space.

7. The sample stage should have 8 wired connections terminating to a vacuum compatible fisher connector on the shroud.

8. For temperature control and monitoring, two calibrated Silicon diode sensors should be provided – one sensor for temperature control and one for sample monitoring. Heating of the sample stage to controllably done using a 50 ohm/watt rugged cartridge heater specified for in vacuum use.

9. A cryogenic 2-channel temperature controller to be provided along with necessary cables and sensor curves loaded.

10. Possibility of site upgradation to DAC with minimal additional hardware. Price to be quoted in optional and shall be considered for evaluation.

11. To be supplied with turbomolecular pump and necessary accessories such as flexible lines/hoses. Turbomolecular pump system should reach an ultimate pressure ~10-6mbar or lower with a backing pump of either oil free diaphragm or scroll pump.

12. A water chiller of suitable capacity of 5TR capacity or more should be provided.

13. The vibrations from the cryocooler head should be completely isolated from the cold head.

14. Main supply: 230 ±10 volts, 50 Hz/ 3Ph, 415V, 50 Hz

15. Installation and student training as required. (No extra cost).

16. At-least 3 similar systems should have been supplied in India in the last 5 years, PO, contact and installation certificates to be provided. Users shall be contacted for feedback.

Name of the firm-

Name of authroized signatory-

Indian Institute of Science Education and Research Bhopal

TECHNO-COMMERCIAL BID

Tender No.: IISERB/SP/2025-26/104 Dated 17.02.2026

Item: Supply and installation Confocal Raman Spectrometer and 4.2K closed cycle optical cryostat.

1 Name of Tendering Company with Registration No. & Date issued by appropriate authorities. (Please enclose copy of certificate of registration)

2 Do you possess trade license issued by Competent Authorities in India? If so, please enclose a copy.

3 Name of Proprietor / Director

4 Furnish following particulars of the Registered Office a. Complete Postal Address

b. Telephone No. / Mob. No.

d. E-Mail Address

5 Furnish following particulars of the Local Branch Office. (if any) a. Complete Postal Address

b. Telephone No. / Mob. No.

d. E-Mail Address

6 PAN No. (Attach Attested Copy)

7 GSTIN No. (Attach Attested Copy)

8 If Manufacturer – Pl. attach the certificate of Registration

9 If Authorised Dealer / Distributer – Pl. attach relevant certificate.

10 Financial turnover for the three financial Years. (Please attach copy of certificate by Chartered Accountant in original )

Financial Year Amount (In Lakhs ) Remarks, if any

(Attach separate sheet if space provided is insufficient)

11 Give details of the major clients – Educational Institutes/Universities, Government Departments, Research Organisations, to whom item/material of same type have been supplied by the bidder during the last five years in the following format.

Sl. No Name & address of the client with details - Name of the contact person, telephone no., Fax no., e-mail id Name & quantity of the items sold Purchase Order Work Order No. & date Purchase order / Work Order amount (Rs. In Lakh)

(If the space provided is insufficient, a separate sheet may be attached)

12 The agency should not have been black listed or banned by any Govt. Department, Government Organization, PSU, University, Autonomous Institute etc. A notarized certificate to this fact should be enclosed with techno-commercial bid as per Annexure-V.

13 Are you an ISO certified manufacturer? If so, please attach a copy of the certificate.

14 Please specify the minimum time required to supply the item / material from the date of receipt of the Purchase Order.

15 Additional information, if any (Attach separate sheet, if required)

16 Agency who is not registered in the Institute Campus Automation System (CAS), get registered via. link: https://payeeregistration.iiserb.ac.in/ If you have already registered, please mention reference code & date. This is for the release of EMD, payment etc., so it is mandatory, without this no payment is possible.

Name of Authorised Signatory:

Name of the Company:

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Tender Inviting Authority: Indian Institute of Science Education and Research Bhopal (IISERB)

Name of Work: Supply andItem: Supply and installation Confocal Raman Spectrometer, and Supply and installation 4.2K closed cycle optical cryostat. installation Confocal Raman Spectrometer,and 4.2K closed cycle optical cryostat

Tender No: IISERB/SP/2025-26/104 dated 17.02.26

Name of the Bidder/ Bidding Firm / Company :

Sl. No. Item Description Item Code / Make Quantity Units Estimated Rate in Rs. P rate Addition / Deduction Addition / Deduction Values Currency Convertion against each Item Quoted Currency in INR / Other Currency BASIC RATE In Figures To be entered by the Bidder in Discounted Offered Any other charges Basic Amount IIIrd Party i.e DGS&D / RITES etc Inspection Charges @0.34%+Service Tax in Rs. P Less for Cenvat Credit,if any respect of Supplies Under full Excise Duty Category in Rs. P GST ( percentage) GST (Amount) RS P Ex-works price FCA price FOB price CIP price (Delhi / Mumbai) FOR IISER BHOPAL Price FCA /FOB Port name HSN Code CIP AirPort

1 Supply and installation Confocal Raman Spectrometer. item1 Set 0.0000 Excess(+) Full Conversion USD Xahsgaf

2 Supply and installation 4.2K closed cycle optical cryostat. item2 Set 0.0000 Excess(+) Full Conversion USD Xahsgaf

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