Chemicals

Monarch® Spin Plasmid Miniprep Kit - 250 preps Quantity: 1 Size: 250 preps Monarch Spin Plasmid Miniprep Kit is a silica column-based system designed for rapid isolation of high-quality plasmid DNA from bacterial cultures. It uses an alkaline lysis method followed by selective binding of plasmid DNA to a spin column, allowing efficient removal of genomic DNA, proteins, and contaminants. The kit provides purified DNA suitable for downstream applications such as sequencing, restriction digestion, and cloning. The procedure is fast, typically completed in under 30 minutes, and does not require phenol or chloroform extraction.

Hi-T7 RNA Polymerase Quantity: 2 Size: 5000 units Hi-T7 RNA Polymerase is a highly active enzyme used for in vitro transcription of RNA from DNA templates containing a T7 promoter. It synthesizes RNA in the 5′→3′ direction and requires Mg²⁺, NTPs, and appropriate buffer conditions for optimal activity. The enzyme is capable of producing high yields of RNA at 37 °C and is widely used for applications such as guide RNA (gRNA) synthesis, mRNA production, and probe generation. It should be stored at -20 °C to maintain stability.