Chemicals

Starch Soluble (500 G) Appearance of the chemical must be White solid powder Solubility should be 33.3 mg soluble in 1 mL of hot water pH (2% in water at 25°C) should be 5.00 - 7.00 Clarity must be 1% in boiling water Residue after ignition should be <= 0.40% Must pass Sensitivity

Coomassive Brilliant Blue R 250 (25 G) Appearance must be Dark Bordeaux to dark purplish-brown or deep blue crystalline powder. Solubility should be 20 ppm in buffer solution Chemical must be in clear solution when in solvent at pH 7.00 DNases must not be detected RNases must not be detected Absorption maxima must be 554 - 563 nm (20 ppm in buffer solution pH 7.00) Loss on drying should be <= 10.00% (at 105°C, 2 hr) Suitability test must be for Protein electrophoresis

Coomassive Brilliant Blue G 250 (25G) Appearance must be (Colour) Dark blue to violet to brown Appearance (Form) should be Crystalline powder Absorbance (A) of chemical at 1% aq. solution (pH 7.0) must be between 520 - 570 Must be Suitable for Electrophoresis

SDS Page Sample Buffer (5x) (Laemmli Buffer 5x) (5ML) Appearance (Form) must be Liquid Appearance (Colour) Must be Dark Blue Solution should be Clear Concentration must be in 5X pH of solution should be 6.8 SDS-PAGE Analysis should have Good resolution of individual proteins and optimum visibility of the tracking dye during electrophoresis.

Glycine for molecular biology (1 KG) Appearance (Colour) must be White Appearance (Form) should be Crystalline powder 10% aq. Solution should be Clear and colourless Assay (NT) must be min. 99.5% 5% aq. Solution must have pH between 5.9 - 6.3 Sulphated Ash must be max. 0.05% Loss on drying Should be max. 0.2% Chloride (CI) must be max. 0.005% Sulphate (SO4) must be max. 0.005% Iron (Fe) must be max. 0.005% Heavy Metals (Pb) must be max. 0.0005% Ammonium (NH4) must be max. 0.0005% Copper (Cu) must max. 0.0005% Homogeneity by TLC should Passes test Absorbance (A) of 1M aq. solution in a 1cm cell v/s H2O @260nm and @280nm should be max. 0.1 Must be free from DNase, RNase and Protease

Micro centrifuge tubes 2ml (1X500) Micro centrifugal tubes must have attached hinged cap. The cap must have a double locking system which provides extra safety while handling precious/ hazardous samples. Frosted surface on the tube for easy labelling. Clarity should be clear without any particles and fibers. Color should be colourless Material must be Polypropylene Graduations must be given Tubes should be autoclavable Height of the tube should be approx. 42mm and Outer Diameter must be approx. 10.75mm Capacity of the tube must be 2.0 mL Temperature Tolerance must Range from -20°C to +121°C

Centrifuge tubes 15 ml (1X100) Tube must be sterile, autoclavable having conical bottom with orange screw cap and solvent resistant Writing area must be large Tubes must be Nuclease and Pyrogen free Tubes must be clear without any spots, scratches and fibers. Color of the Tube must be natural and colourless Color of the Cap should be Orange Material of the tube should be Polypropylene Temperature Tolerance Limit should be -20°C to 121°C Graduations should be from 0.5 to 15ml Cap Diameter must be approx. 30 mm

Centrifuge tubes 50 ml (1X100) Tube must be Sterile, Nuclease free, Pyrogen free and Autoclavable Centrifuge Tube should be 50ml, conical bottom and solvent resistant Clarity should be Clear and color of the tube should be natural Color of the cap must be Orange Material must be made of Polypropylene (Tube) : Polystyrene (Cap) Temperature Tolerance Limit should be -20°C to 121°C Graduations must be from 0.5ml to 3ml, with Graduation interval: 0.5ml and 5ml to 50ml with Graduation Interval: 2.5ml Parameter : 20000xg max. RCF Cap Diameter must be Approx. 35mm

ELISA PLATES – 96 WELL (5X10) Plate must contain 96 wells • Appearance Material Binding surface Maximum Well Capacity • Plate should be clear, translucent and made up of polystyrene. • Binding capacity of IgG/cm2 must be around 300 ~ 400 ng • Medium binding capacity must be 200 ~ 300 ng IgG/cm2. • Maximum capacity of the well must be 400µl • Recommended Working Volume : 350µl • Product alignment must proper grid marking with alphanumerical figure. • Plate should be suitable for use in ELISA Assays.