GeM

AOC

Goods

Tris HCL, For Molecular biology,EDTA, For Molecular biology,NaCl, For Molecular biology,CTAB, For M

BOQ

Purchase of Chemicals

Indian Council Of Forestry Research And Education (icfre)

Bengaluru Urban, Karnataka

Amount: Refer DocsCloses: 26 Feb 2024Closed

Timeline

Published15 Feb 2024
Closed26 Feb 2024
Awarded12 Mar 2024

Bids

4 Bids12 Mar 2024

#	Company	Amount	Rank	Status
1	HIMEDIA LABORATORIES PRIVATE LIMITED L1₹3.8 LQualified 1ST FLOOR 23 VADHANI INDUSTERIAL ESTATE LBS MARG GHATKOPAR MUMBAI MAHARASHTRA 400086 \| MUMBAI SUBURBAN \| MAHARASHTRA \| 400086	₹3.8 L	L1	Qualified MII
2	SPM BIOTECH Disqualified C11 160 GROUND FLOOR SECTOR 3 GROUND FLOOR ROHINI NEW DELHI ROHINI SEC 3 DELHI DELHI 110085 \| NORTH WEST DELHI \| DELHI \| 110085	-	-	Disqualified MII
3	SRI RAM DISTRIBUTORS Disqualified PLOT NO 31 SRI RAM DISTRIBUTORS JCK INDUSTRIAL PARK BELAGOLA INDUSTRIAL AREA MYSORE MYSORE KARNATAKA 570016 \| MYSURU \| KARNATAKA \| 570016	-	-	Disqualified MSE, MII, Category: OBC
4	SUPERWORTH BIO Disqualified 1192 NAR JAIN PARK SHIV MANDIR ADARSH NAGAR SECTOR 14 ADARSH NAGAR SONIPAT HARYANA 131001 \| SONIPAT \| HARYANA \| 131001	-	-	Disqualified MSE, MII, Category: General

Tender Value

Refer Docs

EMD Value

Exempted

Closing Date

26 Feb 2024, 12:00 pmClosed

Work Description

Purchase of Chemicals

Tender Information

Tender ID

6065309

Reference Number

GEM/2024/B/4638284

Tender Type

Two Packet Bid

Product Category

Tris HCL, For Molecular biology,EDTA, For Molecular biology,NaCl, For Molecular biology,CTAB, For M

Form of Contract

GeM Contract

Period of Work

15 days

Location

Karnataka; Bengaluru Urban

Evaluation Method

Total value wise evaluation

Bid Type

BOQ

Source Website

Subscribe to view

Awarded Items

GEMC-511687756402597

Awarded to HIMEDIA LABORATORIES PRIVATE LIMITED

₹3.8 L

Products

Product	Brand / Model	HSN	Quantity	Unit Price	Amount
Tris HCL, For Molecular biology	HiMedia / MB030-500G	HSN not specified by seller	3 500g	4,237.68	12,713.04
EDTA, For Molecular biology	HiMedia / MB011-500G	HSN not specified by seller	2 500g	1,429.28	2,858.56
NaCl, For Molecular biology	HiMedia / MB023-500G	HSN not specified by seller	2 500g	739.5	1,479
CTAB, For Molecular biology	HiMedia / MB101-500G	HSN not specified by seller	3 500g	2,246.72	6,740.16
Chloroform, For Molecular biology	HiMedia / MB109-500ML	HSN not specified by seller	2 500ml	1,800.39	3,600.78
Saturated phenol, For Molecular biology	HiMedia / MB082-500ML	HSN not specified by seller	1 500ml	9,247.66	9,247.66
Agarose, For Molecular biology	HiMedia / MB002-500G	HSN not specified by seller	6 500g	15,440.25	92,641.5
DNA extraction KIT For Molecular biology	HiMedia / MB571-50PR	HSN not specified by seller	6 50preps	7,882.56	47,295.36
Tris free base, For Molecular biology	HiMedia / MB029-1KG	HSN not specified by seller	2 1 Kg	6,880.58	13,761.16
Sucrose, For Molecular biology	HiMedia / MB025-500G	HSN not specified by seller	2 500g	556.92	1,113.84
Perchloric acid AR, Bio chemicals	HiMedia / AS013-500ML	HSN not specified by seller	1 500ml	2,236.69	2,236.69
Chloroform AR, Bio chemicals	HiMedia / AS040-500ML	HSN not specified by seller	3 500ml	697.09	2,091.27
Sulphuric acid AR	HiMedia / AS016-500ML	HSN not specified by seller	3 500ml	546.64	1,639.92
Acetic acid glacial AR	HiMedia / AS001-500ML	HSN not specified by seller	2 500ml	516.55	1,033.1
Citric acid anhydrous - AR	HiMedia / GRM1023-500G	HSN not specified by seller	1 500g	752.25	752.25
Sodium carbonate - anhydrous TC grade	HiMedia / GRM851-500G	HSN not specified by seller	1 500g	466.4	466.4
Hydrochloric acid	HiMedia / AS004-500ML	HSN not specified by seller	2 500ml	451.35	902.7
Disodium hydrogen phosphate	HiMedia / GRM3961-500G	HSN not specified by seller	1 500g	722.16	722.16
Acetone AR	HiMedia / AS025-500ML	HSN not specified by seller	5 500ml	631.89	3,159.45
Methanol AR	HiMedia / AS059-500ML	HSN not specified by seller	5 500ml	416.25	2,081.25
Agar agar Type I	HiMedia / GRM666-500G	HSN not specified by seller	16 500g	4,814.4	77,030.4
Agar PT pure	HiMedia / RM7695-500G	HSN not specified by seller	6 500g	6,374.07	38,244.42
Hoagland Number 2 Basal salt mixture Plant tissue grade	HiMedia / TS1094-5L	HSN not specified by seller	1 5 litres	406.22	406.22
MS nitrate supplement, for plant tissue culture	HiMedia / PT778-50L	HSN not specified by seller	4 50 litres	466.4	1,865.6
Phytacap for plant tissue culture	HiMedia / PLA008-1x500NO	HSN not specified by seller	10 500g	3,640.89	36,408.9
Cephotaxime sodium salt, for plant tissue culture	HiMedia / PCT1103-5G	HSN not specified by seller	1 5g	6,128.33	6,128.33
Kanamycin sulphate for plant tissue culture	HiMedia / TC136-5G	HSN not specified by seller	1 5g	4,754.22	4,754.22
Hygromycin-B For plant tissue culture	HiMedia / PCT1104-100MG	HSN not specified by seller	1 100mg	3,981.91	3,981.91
Putrescine dihydrochloride For plant tissue culture	HiMedia / PCT0811-5G	HSN not specified by seller	1 5g	2,031.08	2,031.08
m-topolin for plant tissue culture	HiMedia / PCT0827-25MG	HSN not specified by seller	1 25mg	5,416.2	5,416.2

Documents Required

7 documents required · 7 mandatory

RequiredExperience Criteria

RequiredPast Performance

RequiredBidder Turnover

RequiredCertificate (Requested in ATC)

RequiredOEM Authorization Certificate

RequiredOEM Annual Turnover

RequiredCompliance of BoQ specification and supporting document

Eligibility Criteria

Experience

3 yrs

Min Turnover

₹3

Past Performance

50%

Purchase Preferences

MSE Purchase PreferenceMSE EMD ExemptionMII Purchase PreferenceStartup Exemption

Fee & Financial Details

EMD Fee Amount

Exempted

Important Dates

Last Activity

12 Mar 2024

Published

15 Feb 2024

Bid Opening

26 Feb 2024

Bill of Quantities

Item Wise

GeM BOQ Items (30 items)

Item No	Item Title	Description	Qty	Unit	Consignee	Delivery (days)
1	Tris HCL, For Molecular biology	Tris hydroxymethyl aminomethane hydrochloride for Molecular Biology, CAS Number 1185 53 1, EC number 214 684 5, HS code 2922 19 90, Molecular Formula C4H11NO3. HCl, Molecular Weight 157.60, storage Below 30 degree C, Shelf life 4 years, White hygroscopic crystals or solid or powder with Solubility 33.3 mg soluble in 1 mL of water and pH 0.5M in water at 25 degree C to be 3.50 to 5.00, Assay NaOH Titration 99.00 - 102.00 percent, No detections of DNases and RNases, FTIR Matches with the standard pattern	3	500g	[email protected]	15
2	EDTA, For Molecular biology	For Molecular Biology ,CAS Number 638 19 26, Molecular Formula C10H14N2Na2O8.2H2O, EC 205 358 3, HS code 2922 49 90, Molecular Weight 372.24, Storage Below 30 degree C, Shelf life 4 years White crystals or powder with Solubility 33.3 mg soluble in 1 mL of water, and pH 5 percent in water at 25 degree C to be 4.00 to 6.00, Loss on drying at 150 degree C 6 hour is 7.00 to 11.0 percent, Assay ZnCl2 Titration on dry basis 99.00 to 101.50 percent, No detections of DNases and RNases	2	500g	[email protected]	15
3	NaCl, For Molecular biology	For Molecular Biology, CAS Number 7647 14 5, EC number 231 598 3, HS code 2501 00 90, Molecular Formula NaCl, Molecular Weight 58.44, Storage Below 30 degree C, Shelf life 4 years, colourless to white crystals or powder or solid, Solubility 100 mg soluble in 1 mL of water, pH 5 percent in water at 25 degree C to be 5.00 to 9.00 Assay AT 99.50 to 102.00 percent, No detections of DNases and RNases	2	500g	[email protected]	15
4	CTAB, For Molecular biology	For Molecular Biology, N-Cetyl-N,N,N-trimethylammonium bromide, CAS Number 57 09 0, EC number 200 311 3, HS code 2923 90 00, Molecular Formula C19H42Br N, Molecular Weight 364.45Storage below 30 degree C, Shelf life 4 years, White crystals or powder, Solubility 33.3 mg soluble in 1 mL of methanol and pH (0.1 M in water at 25 degree C) to be 5.00 to 7.00, Melting range 248 - 251 degree C, Water K.F. less than equal to 1.00 percent, Assay (NT/AT, on anhydrous basis) 99.00 to 101.00 percent, No detections of DNases and RNases, FTIR Matches with the standard pattern	3	500g	[email protected]	15
5	Chloroform, For Molecular biology	For Molecular Biology, CAS number 67 66 3, EC number 200 663 8 , EC index number 602 006 004, HS code 2903 13 00, Molecular Formula CHCl3, Molecular Weight 119.38, Storage below 30 degree C, Shelf life 4 years, Colourless liquid, Solubility 1 mL miscible with 1 mL of alcohol, Refractive index (n 20/D) to be 1.4420 to 1.4450, Density at 25 degree C 1.474 to 1.480g/mL Magnesium less than equal to 0.00002 percent, Calcium less than equal to 0.00005 percent, Iron less than equal to 0.00005 percent, Manganese less than equal to 0.000005 percent, Cadmium less than equal to 0.000005 percent, Assay (GC) min. 99.80 percent, No detections of DNases and RNases, FTIR Matches with the standard pattern	2	500ml	[email protected]	15
6	Saturated phenol, For Molecular biology	For Molecular Biology, Phenol solution saturated with 10mM Tris HCl pH 8.0 with 1mn EDTS. CAS Number 108 95 2, EC number 203 632 7, EC index number 604 001 00 2, HS code 3822 00 90, Molecular Formula C6H6O Molecular Weight 94.11, Storage at 2 to 8 degree C, Shelf life 2 years, Colourless to yellow liquid capped with a layer of Tris buffer. pH of aqueous layer after extraction 7.70 to 8.10, Assay (GC) min. 99.00 percent, No detections of DNases and RNases	1	500ml	[email protected]	15
7	Agarose, For Molecular biology	For Molecular Biology, CAS number 9012 36 6, EC number 232 731 8, HS code 3913 90 90, Storage below 30 degree C, Shelf life 4 years, White to off-white crystals or powder. Solubility 10 mg soluble in 1 mL of hot water, Gel point (1.50 percent gel) 34. 50 to 37.50 degree C, Gel strength (1 percent gel) min. 1200 gm/cm2, Sulphated ash less than equal to 0.50 percent, Loss on drying (at 105 degree C, 2 hr) less than equal to 10.00 percent, Electroendosmosis (-MR) 0.09 to 0.13 percent, No detections of DNases and RNases.	6	500g	[email protected]	15
8	DNA extraction KIT For Molecular biology	For Molecular Biology, this kit simplifies isolation of DNA from fresh plant material with spin column procedure. The procedure is optimized for a maximum of 200 mg of wet weight of the starting material. The sample (fresh) is cut and ground in liquid nitrogen along with Super Plant Extraction Buffer. Super Plant Extraction Buffer contains CTAB Cetyltrimethylammonium bromide, a detergent used to break open plant cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform isoamyl alcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A. The flow through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column Capped that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit.	6	50preps	[email protected]	15
9	Tris free base, For Molecular biology	For Molecular Biology, Tris hydroxymethyl aminomethane CAS number 77 86 1, EC number 201 604 4, HS code 2922 19 20, Molecular Formula C4H11NO3, Molecular Weight 121.14, Storage below 30 degree C, Shelf life 4 years, Colourless or white to off-white crystals or powder or chunks or solid, 100 mg soluble in 1 mL of water, pH to be 10.00 to 11.50, melting range 162 to 176 degree C Water less than equal to 2.00 percent, assay titration on dry basis greater than equal to 99.00 percent No detections of DNases and RNases. FTIR Matches with the standard pattern	2	1 Kg	[email protected]	15
10	Sucrose, For Molecular biology	For Molecular Biology, CAS Number 57 50 1, Molecular Formula C12H22O11, EC number 200 334 9, HS code 1701 99 90, Molecular Weight 342.30, Storage below 30 degree C, Solubility 1000 mg soluble in 1 mL of water. Melting range 185 to 187 degree C, Specific rotation plus 66.0 to plus 67.0 degree c is equal to 26 percent in water, at 20 degree C, Assay GC/HPLC min. 99.50 percent, Shelf life 4 years. No detections of DNases and RNases. FTIR Matches with the standard pattern	2	500g	[email protected]	15

Stage Documents

Award of Contract

contract_GEMC-511687756402597.pdf

GEM_CONTRACT • 0.17 MB

Documents

Tap a document below to read it instantly. You can also download everything as a ZIP if you prefer.

Tender Documents

bid_6065309.pdf

GEM_BID

BOQ Documents

specifications_for_chemicals_2024-02-15-11-31-17_70ee128fd32f2501526e670fbc7291f7.pdf

BOQ

boq_item_sample_file_2024-02-15-11-31-17_b748531b80d0c30296a3e77a6adfd7b2.csv

BOQ

Work Item Documents

gtc.pdf

OTHER

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Company

HIMEDIA LABORATORIES PRIVATE LIMITED

L1₹3.8 LQualified

1ST FLOOR 23 VADHANI INDUSTERIAL ESTATE LBS MARG GHATKOPAR MUMBAI MAHARASHTRA 400086 | MUMBAI SUBURBAN | MAHARASHTRA | 400086

SPM BIOTECH

Disqualified

C11 160 GROUND FLOOR SECTOR 3 GROUND FLOOR ROHINI NEW DELHI ROHINI SEC 3 DELHI DELHI 110085 | NORTH WEST DELHI | DELHI | 110085

SRI RAM DISTRIBUTORS

Disqualified

PLOT NO 31 SRI RAM DISTRIBUTORS JCK INDUSTRIAL PARK BELAGOLA INDUSTRIAL AREA MYSORE MYSORE KARNATAKA 570016 | MYSURU | KARNATAKA | 570016

SUPERWORTH BIO

Disqualified

1192 NAR JAIN PARK SHIV MANDIR ADARSH NAGAR SECTOR 14 ADARSH NAGAR SONIPAT HARYANA 131001 | SONIPAT | HARYANA | 131001

Product

Brand / Model

HSN

Quantity

Unit Price

Amount

Tris HCL, For Molecular biology

HiMedia / MB030-500G

HSN not specified by seller

3 500g

4,237.68

12,713.04

EDTA, For Molecular biology

HiMedia / MB011-500G

HSN not specified by seller

2 500g

1,429.28

2,858.56

NaCl, For Molecular biology

HiMedia / MB023-500G

HSN not specified by seller

2 500g

739.5

1,479

CTAB, For Molecular biology

HiMedia / MB101-500G

HSN not specified by seller

3 500g

2,246.72

6,740.16

Chloroform, For Molecular biology

HiMedia / MB109-500ML

HSN not specified by seller

2 500ml

1,800.39

3,600.78

Saturated phenol, For Molecular biology

HiMedia / MB082-500ML

HSN not specified by seller

1 500ml

9,247.66

Agarose, For Molecular biology

HiMedia / MB002-500G

HSN not specified by seller

6 500g

15,440.25

92,641.5

DNA extraction KIT For Molecular biology

HiMedia / MB571-50PR

HSN not specified by seller

6 50preps

7,882.56

47,295.36

Tris free base, For Molecular biology

HiMedia / MB029-1KG

HSN not specified by seller

2 1 Kg

6,880.58

13,761.16

Sucrose, For Molecular biology

HiMedia / MB025-500G

HSN not specified by seller

2 500g

556.92

1,113.84

Perchloric acid AR, Bio chemicals

HiMedia / AS013-500ML

HSN not specified by seller

1 500ml

2,236.69

Chloroform AR, Bio chemicals

HiMedia / AS040-500ML

HSN not specified by seller

3 500ml

697.09

2,091.27

Sulphuric acid AR

HiMedia / AS016-500ML

HSN not specified by seller

3 500ml

546.64

1,639.92

Acetic acid glacial AR

HiMedia / AS001-500ML

HSN not specified by seller

2 500ml

516.55

1,033.1

Citric acid anhydrous - AR

HiMedia / GRM1023-500G

HSN not specified by seller

1 500g

752.25

Sodium carbonate - anhydrous TC grade

HiMedia / GRM851-500G

HSN not specified by seller

1 500g

466.4

Hydrochloric acid

HiMedia / AS004-500ML

HSN not specified by seller

2 500ml

451.35

902.7

Disodium hydrogen phosphate

HiMedia / GRM3961-500G

HSN not specified by seller

1 500g

722.16

Acetone AR

HiMedia / AS025-500ML

HSN not specified by seller

5 500ml

631.89

3,159.45

Methanol AR

HiMedia / AS059-500ML

HSN not specified by seller

5 500ml

416.25

2,081.25

Agar agar Type I

HiMedia / GRM666-500G

HSN not specified by seller

16 500g

4,814.4

77,030.4

Agar PT pure

HiMedia / RM7695-500G

HSN not specified by seller

6 500g

6,374.07

38,244.42

Hoagland Number 2 Basal salt mixture Plant tissue grade

HiMedia / TS1094-5L

HSN not specified by seller

1 5 litres

406.22

MS nitrate supplement, for plant tissue culture

HiMedia / PT778-50L

HSN not specified by seller

4 50 litres

466.4

1,865.6

Phytacap for plant tissue culture

HiMedia / PLA008-1x500NO

HSN not specified by seller

10 500g

3,640.89

36,408.9

Cephotaxime sodium salt, for plant tissue culture

HiMedia / PCT1103-5G

HSN not specified by seller

1 5g

6,128.33

Kanamycin sulphate for plant tissue culture

HiMedia / TC136-5G

HSN not specified by seller

1 5g

4,754.22

Hygromycin-B For plant tissue culture

HiMedia / PCT1104-100MG

HSN not specified by seller

1 100mg

3,981.91

Putrescine dihydrochloride For plant tissue culture

HiMedia / PCT0811-5G

HSN not specified by seller

1 5g

2,031.08

m-topolin for plant tissue culture

HiMedia / PCT0827-25MG

HSN not specified by seller

1 25mg

5,416.2

Item No

Item Title

Description

Qty

Unit

Consignee

Delivery (days)

Tris HCL, For Molecular biology

Tris hydroxymethyl aminomethane hydrochloride for Molecular Biology, CAS Number 1185 53 1, EC number 214 684 5, HS code 2922 19 90, Molecular Formula C4H11NO3. HCl, Molecular Weight 157.60, storage Below 30 degree C, Shelf life 4 years, White hygroscopic crystals or solid or powder with Solubility 33.3 mg soluble in 1 mL of water and pH 0.5M in water at 25 degree C to be 3.50 to 5.00, Assay NaOH Titration 99.00 - 102.00 percent, No detections of DNases and RNases, FTIR Matches with the standard pattern

500g

[email protected]

EDTA, For Molecular biology

For Molecular Biology ,CAS Number 638 19 26, Molecular Formula C10H14N2Na2O8.2H2O, EC 205 358 3, HS code 2922 49 90, Molecular Weight 372.24, Storage Below 30 degree C, Shelf life 4 years White crystals or powder with Solubility 33.3 mg soluble in 1 mL of water, and pH 5 percent in water at 25 degree C to be 4.00 to 6.00, Loss on drying at 150 degree C 6 hour is 7.00 to 11.0 percent, Assay ZnCl2 Titration on dry basis 99.00 to 101.50 percent, No detections of DNases and RNases

500g

[email protected]

NaCl, For Molecular biology

For Molecular Biology, CAS Number 7647 14 5, EC number 231 598 3, HS code 2501 00 90, Molecular Formula NaCl, Molecular Weight 58.44, Storage Below 30 degree C, Shelf life 4 years, colourless to white crystals or powder or solid, Solubility 100 mg soluble in 1 mL of water, pH 5 percent in water at 25 degree C to be 5.00 to 9.00 Assay AT 99.50 to 102.00 percent, No detections of DNases and RNases

500g

[email protected]

CTAB, For Molecular biology

For Molecular Biology, N-Cetyl-N,N,N-trimethylammonium bromide, CAS Number 57 09 0, EC number 200 311 3, HS code 2923 90 00, Molecular Formula C19H42Br N, Molecular Weight 364.45Storage below 30 degree C, Shelf life 4 years, White crystals or powder, Solubility 33.3 mg soluble in 1 mL of methanol and pH (0.1 M in water at 25 degree C) to be 5.00 to 7.00, Melting range 248 - 251 degree C, Water K.F. less than equal to 1.00 percent, Assay (NT/AT, on anhydrous basis) 99.00 to 101.00 percent, No detections of DNases and RNases, FTIR Matches with the standard pattern

500g

[email protected]

Chloroform, For Molecular biology

For Molecular Biology, CAS number 67 66 3, EC number 200 663 8 , EC index number 602 006 004, HS code 2903 13 00, Molecular Formula CHCl3, Molecular Weight 119.38, Storage below 30 degree C, Shelf life 4 years, Colourless liquid, Solubility 1 mL miscible with 1 mL of alcohol, Refractive index (n 20/D) to be 1.4420 to 1.4450, Density at 25 degree C 1.474 to 1.480g/mL Magnesium less than equal to 0.00002 percent, Calcium less than equal to 0.00005 percent, Iron less than equal to 0.00005 percent, Manganese less than equal to 0.000005 percent, Cadmium less than equal to 0.000005 percent, Assay (GC) min. 99.80 percent, No detections of DNases and RNases, FTIR Matches with the standard pattern

500ml

[email protected]

Saturated phenol, For Molecular biology

For Molecular Biology, Phenol solution saturated with 10mM Tris HCl pH 8.0 with 1mn EDTS. CAS Number 108 95 2, EC number 203 632 7, EC index number 604 001 00 2, HS code 3822 00 90, Molecular Formula C6H6O Molecular Weight 94.11, Storage at 2 to 8 degree C, Shelf life 2 years, Colourless to yellow liquid capped with a layer of Tris buffer. pH of aqueous layer after extraction 7.70 to 8.10, Assay (GC) min. 99.00 percent, No detections of DNases and RNases

500ml

[email protected]

Agarose, For Molecular biology

For Molecular Biology, CAS number 9012 36 6, EC number 232 731 8, HS code 3913 90 90, Storage below 30 degree C, Shelf life 4 years, White to off-white crystals or powder. Solubility 10 mg soluble in 1 mL of hot water, Gel point (1.50 percent gel) 34. 50 to 37.50 degree C, Gel strength (1 percent gel) min. 1200 gm/cm2, Sulphated ash less than equal to 0.50 percent, Loss on drying (at 105 degree C, 2 hr) less than equal to 10.00 percent, Electroendosmosis (-MR) 0.09 to 0.13 percent, No detections of DNases and RNases.

500g

[email protected]

DNA extraction KIT For Molecular biology

For Molecular Biology, this kit simplifies isolation of DNA from fresh plant material with spin column procedure. The procedure is optimized for a maximum of 200 mg of wet weight of the starting material. The sample (fresh) is cut and ground in liquid nitrogen along with Super Plant Extraction Buffer. Super Plant Extraction Buffer contains CTAB Cetyltrimethylammonium bromide, a detergent used to break open plant cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform isoamyl alcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A. The flow through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column Capped that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit.

50preps

[email protected]

Tris free base, For Molecular biology

For Molecular Biology, Tris hydroxymethyl aminomethane CAS number 77 86 1, EC number 201 604 4, HS code 2922 19 20, Molecular Formula C4H11NO3, Molecular Weight 121.14, Storage below 30 degree C, Shelf life 4 years, Colourless or white to off-white crystals or powder or chunks or solid, 100 mg soluble in 1 mL of water, pH to be 10.00 to 11.50, melting range 162 to 176 degree C Water less than equal to 2.00 percent, assay titration on dry basis greater than equal to 99.00 percent No detections of DNases and RNases. FTIR Matches with the standard pattern

1 Kg

[email protected]

Sucrose, For Molecular biology

For Molecular Biology, CAS Number 57 50 1, Molecular Formula C12H22O11, EC number 200 334 9, HS code 1701 99 90, Molecular Weight 342.30, Storage below 30 degree C, Solubility 1000 mg soluble in 1 mL of water. Melting range 185 to 187 degree C, Specific rotation plus 66.0 to plus 67.0 degree c is equal to 26 percent in water, at 20 degree C, Assay GC/HPLC min. 99.50 percent, Shelf life 4 years. No detections of DNases and RNases. FTIR Matches with the standard pattern

500g

[email protected]