Supply, Testing and Installation of Laboratory Items in the Watson-Crick Centre for Molecular Medicine

Bio-safety cabinet (4 FT) (Thermo-Fisher, Berner, and Eppendorf) Class II Bio-safety cabinet, type A2, open-front, ventilated cabinet in ergonomic design vertical type. Size: approx. 4 ft and Cabinet made of steels (work zone - stainless steel and side walls-electro galvanized steel) with antimicrobial coating Should have support stand (Adjustable height) with caster wheels for 4 ft cabinet ULPA/HEPA-filtered, re-circulated mass airflow with in the work space ULPA/HEPA filter with minimum 99% efficiency against 0.3μm particles. Exhaust air from the cabinet should also filtered by HEPA filters Motor should be Dual DC & must automatically adjust the airflow speed (balancing inflow and down flow) without the use of a damper to ensure continuous safe working conditions. Average air flow should be 90 ± 20 fpm UV light must be programmable to allow for specific exposure times from 0 to 24 hours. Lightening power should >1000 Lux (100fc); cabinet noise level must be less than 65 dB. Low noise (< 65 db), low energy consumption and heat output Microprocessor controlled functions with LCD display Audible and visual alarms Machine should be Energy Efficient with Power Consumption in Normal mode: 250W ±15% Electric supply: 220-240V, AC, 50Hz European CE/FDA and NSF/ANSI 49 certification Two plugged cable ports, one on each side wall

Digital pH meter (Sartorius, Mettler Toledo, Fisher Scientific) Should have Working range from 0 to 14. Resolution 0.1/0.01 pH. Should have RS.232C output and supply Data connector cable. Automatic calibration facility. Data storage facility and record maximum and minimum value. 3 stage calibration. Digital display with 0.001 pH unit readability Tri-combination pH/ATC electrode Supply Electrode + Standard buffer solution (pH 4.0, 7.0, 10.01 x 50ml for each bottle) + standard electrode holder +Ac /DC Adaptor. Should be provided with an electrode holder/arm with smooth movement Temperature compensation zero to 100 degree C with ATC

Automatic X-Ray Film Processor (Tarsons, Genetix and Thermo- Fisher, Himedia, Agilent) Table top processor with consistent, high-quality processing of films of up to 36 cms width. High-temperature, high-speed developing and fixing bath Developing tank 5 -6 liters, fixing tank 5-6 liters, washing tank 5-6 liters, and replenishment tank- 25liters Developing time: less than 1 mins Water Consumption of about 1-2 liters/min Should be supplied with 10-15 developing cassettes of standard size. Power Supply: AC220V, 50/60HZ European CU Certified

CO2 Incubator (Thermo-Fisher, Berner, and Eppendorf) Microprocessor controlled CO2 Incubator with work chamber volume approximately within range of 150-190 liters Water jacket CO2 incubators with corrosion-resistant and stainless steel chamber. CO2 range 0-20%, with ±0.1% accuracy Temperature range: ambient to 55°C with temp accuracy: ±0.1°C HUMIDITY: Ambient to 95% @ 37°C (98.6°F) System should have interactive control to access rapidly important information for each critical parameter in the incubator Temperature, CO2 and humidity recovery time should be very fast System should have built-in moist or dry heat decontamination (sterilization) facility to remove bacteria, fungi, spores, mycoplasma etc. At least 3 nos. of stainless steel perforated shelves should be supplied Interior chamber should be made of stainless steel. Unit should have appropriate filter (HEPA etc.) Infrared (IR) CO2 Sensor (5 units); TCD sensor (10 units). Those 5 units equipped with IR based CO2 sensor should be able to adjust baseline automatically The system should have large digital display for both Temp. & CO2 simultaneously. System should have at least two doors, one main door and one glass doors. It should be ISO 9001 & CE Certification. Suitable for 230V, single phase 50 Hz operation.

UV Trans- illuminator (Tarsons, Genetix and Thermo Fisher, Himedia, Agilent) Light Source: 4 x 8 Watt UV bulbs Wavelength:302nm midrange Intensity Control: Low (50%) or High (100%) Viewing Surface: 14-16 x16-18cm Ext. Dimensions: 10.5 x 13 x 5.5 in / 26.5 x 32.5 x 14 cm Hinged UV safety cover

Dry Block Heaters Accurate temperature control from ambient +5ºC to 150ºC in 0.1ºC increments Integral aluminium/stainless steel block to hold 12 x 1.5-2 ml tubes Temperature increments: 0.1°C Timer: 0 - 19 hours 59 minutes or continuous Electrical : 100 - 240V / 50-60 Hz

Hotplate Magnetic Stirrer (Tarsons, Genetix and Thermo Fisher, Himedia, Agilent) Power [W]:L 520-550 Max. stirring quantity（H2O）[L]: 10-20 Motor type: External rotor brushless motor Motor rating input [W]: 18 Motor rating output [W]: 10 Speed range [rpm]: 0-1500 Dimension of Plate [mm]: φ135 Heating power [W]: 500 Heat rate（1Lwater）[K/min]: 4-6 Heating Temperature Range [0C]: 25-340 Dimension [mm]: 280×160×85

Water Bath (Tarsons, Genetix and Thermo Fisher, Himedia, Agilent) Temperature range: Ambient +2°C to 100°C Accuracy: ±1°C TIMER: 99 hours and 59 minutes in 1 minute increments, or for continuous operation HEATER: 1500 watts Bath Capacity: 30 - 38 liters Voltage: 220 volt, 50 Hz Rating Watts : 250-500

Ice Flaker Machine Capacity of Ice Flake : 30-60 Kg. Storage bin : ABS Plastic Storage Capacity (Kg.) >=10 Kg Cooling Method : Air Working temp. : below 30ºC Water Supply : 3/4 inch BSP Fully Automatic microprocessor control Compact Design and low maintenance Stainless Steel construction Continuous Ice Flakes Output, No cubes Noiseless operation with noise level : ≤60 dB CFC free compressor Overload protection and Full bin cutoff sensor Low water level detection Wheels for easy mobility Warranty 24 months Power Supply : 220V/50Hz

Spinix Vortex. (Tarsons, Genetix and Thermo Fisher, Himedia, Agilent) Vortex should have Speed control Vortex should have Spare CUP Attachment Vortex should have Spare One hand attachment Vortex should have Spare Microtube insert.

Phase Contrast Microscope for Tissue culture (Eppedndorff and Thermo Fisher, Himedia, Agilent) Microscope should have Simple software and hardware installation Should have Manual mechanical stage should not need to have Fluorescence channels Should have Monochrome or color camera option Should be Benchtop system and suitable for tissue culture hood Microscope should not need darkroom Should have USB ports to transfer data Should have Cell analysis software Should have Networking capability Should fit into the Laminar Hood Should have Adjustable intensity LED Illumination Optics: Should have Epifluorescence, transmitted light (brightfield and Contrast methods Should have Objective turret with Objectives 4x-10x-20x-40x-60x-100X Should have Mechanical focus wheel with single knob for coarse and fine focus Should have Mechanical X/Y stage Should have LCD display with 18.5" articulated LCD color monitor 1920x1080 pixel resolution

1.5 ml centrifuge (Eppedorff and Thermo Fisher, Himedia, Agilent) Centrifuge should have Rotor capacity of 48 × 1.5/2.0 mL, 12 × 5.0 mL Centrifuge should Max. speed 25,000 × g (16,220 rpm): Centrifuge should have Soft touch one-finger closure for ergonomic operation Centrifuge should have Temperature range from 11 °C to 40 °C Centrifuge should have Temp working function between ~ 23 °C to 4 °C Centrifuge should have programmed to shut-off after 1, 2,4 or 8 hours Centrifuge should have in built-in condensation drains to eliminate water accumulation Centrifuge should have an Automatic rotor recognition and imbalance detection for maximized operational safety Centrifuge s should have Max. capacity 48 × 1.5/2.0 mL Centrifuge s should have Acceleration time 15-20 s Centrifuge s should Deceleration time 15-20 s Centrifuge should have Large Display Large LCD Centrifuge should have Noise level < 52 db Centrifuge should have Power supply 115-230 V, 50 Hz Max. power 550 W consumption Centrifuge should have Dimensions31.9 × 54.0 × 25.4 cm / 12.6 × 21.3 × 10 in Centrifuge should have Weight w/o accessories30 kg / 66.1 lb Centrifuge should have refrigerated Cooling Centrifuge should have Temperature control range

Microwave Should be energy efficient with 3-5-star power efficiency Should have 1-2-year warranty 30L Capacity Control: Touch keypad (membrane) is sensitive to touch and easy to clean Should have capacity to store 20 programs

Rocky Max (Tarsons, Genetix and Thermo Fisher, Himedia, Agilent) Speed 10-50 RPM Time can withhold speed 99 hr and 99 minutes Platform Dimension 30 L X 30 B in (cm) Maximam Load 20 kg Load AC, 230V, 50 Hz

RotoSpin disk (1.5 ml (Tarsons, Genetix and Thermo Fisher, Himedia, Agilent) Disk for 24 (1.5ml) Eppendorf tubes Speed 10-100 RPM Must have 1-3 years warranty Load AC, 230V, 50 Hz Time can withhold speed 99 hr and 99 minutes Should have LCD with touch down for control the speed Should work under between 4-30 degree

Agarose gel Electrophoresis Unit (Tarsons, Sigma and Thermo Fisher) Gel Tank should have a volume of 400-500 ml and compatible homemade gels Gel Tank should accommodate up to two or more gels per run. Should supply power Supply Adapters to connect the covered power leads of electrophoresis units to power supplies made by other companies Should supply gel Handcast System. There should be at least 2-Handcast Stations Power supply should have constant voltage or current settings Power supply should have Built-in timer for walk-away gel electrophoresis Power supply should have 2-4 sets of output jacks that are compatible with most electrophoresis devices. Two Boats (dams) large and small Handcast System should include: Gel Combs at least 2 each: 5, 10 and 15 well. Suitable for electric supply of 220-240V European CE certification Warrant ≥1 year and from the date of installation and AMC per year after the expiry of standard warranty

M/S SHA INFRA

BIOMED SYSTEMS

BIOMED SYSTEMS

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