BT/PR53954/CON3

Amount

pGEMT Vector sytemA1360, Promega or equivalent

PureYield Plasmid Miniprep systemA1222, Promega or equivalent

GoTaq Flexi DNA PolymeraseM8295, Promega or equivalent

M-MLV Reverse TranscriptaseM1705, Promega or equivalent

T4 DNA LigaseM1794, Promega or equivalent

Protein ladderab116028, Abcam or equivalent

Agarose 50004, Lonza or equivalent

Anti-6X His tag antibodyab125262, Abcam or Equivalent

GoScript™ Reverse Transcription SystemA5000, Promega or equivalent

Anti GFP AntibodyA290, antibodies.com or equivalent

Primers with HPLC Purification, IDT or equivalent

Streptavidin MagneSphere Paramagnetic ParticlesZ5481, Promega or equivalent

Wizard® SV Gel and PCRA9281, Promega or equivalent

FuGENE HD TransfectionE2311, promega or equivalent

Paul Scientrument (BID ID -6632097)

SYNGENX INDIA (BID ID -6636419)