SITC of DNA Sequencer

PUNJAB BIOTECHNOLOGY INCUBATOR

COMMERCIAL BID FORMAT - DNA Sequencer

Name of the Manufacturer

Make of the Equipment

Rates to be Quoted Option 1 - Rate with 4 years Standard Warranty Option 2 - Rate with 6 years Standard Warranty

Part A - Main Equipment Qty Rate (in Rs.) GST Amount (In Rs.) Total Amount (in Rs.) Rate (in Rs.) GST Amount (In Rs.) Total Amount (in Rs.)

Application: Genetic Fidelity testing for Seed Potato, Basmati Rice Authentication, Fragment analysis & Genomic Sequencing, de novo sequencing, Gene Expression, Targeted Sequencing (Variant Validation). System: High end, Bench top DNA Sequencer with 24 capillaries.

1 System should be capable of running ‘one polymer one array’ for both sequencing and Fragment analysis applications.

2 Instrument should be fully automated, 24 capillary, fluorescence-based genetic analysis system to process multiple samples in single run.

3 System should be capable of supporting 4 plates; accommodate 96 well Standard and Fast plates or 384 well plates; 8-tube Standard or Fast strips.

4 System should be capable of continuous plate loading with complete walk away automation.

5 System must be built with a solid-state long-life laser.

6 The system must be able to detect and analyse multiple (at least 6) fluorescent dyes simultaneously.

7 System should have peltier-cooled charged coupled device (CCD) detection technology and a spectrograph for multicolor detection.

8 System should be capable of doing Sanger sequencing applications de novo, Long Read Sequencing, indels, and fragment analysis applications including Microsatellite, SSCP, HMA (Heteroduplex Mobility Assay), SNP validation and screening.

9 The system should have an integrated touchscreen that enables for stand-alone instrument control, data collection, quality control monitoring and auto-analysis of data.

10 System should have a module for remote monitoring and data visualization via a mobile device or networked device.

11 Flexible connectivity should be available via Local Area Network (LAN) and should be LIMS compatible.

12 System should have a smart inbuilt barcode reading mechanism facilitating tracking of samples using barcoded plates, and auto-links plate files without the need of an external barcode reader.

13 The system should be supplied with a thermal cycler for DNA amplification, fluorometer for nucleic acid & protein quantification and a hybridization oven for nucleic acid hybridization applications.

14 Software for secondary data analysis should come from original equipment manufacturer.

15 System software should allow real-time data quality evaluation.

16 System should be supplied with a licensed Suite Browser based platform software, Enhanced SAE console, Machine Learning Based base calling, Automation of processes, Variant Detection (Ref <100bp), Customized Reporting like features.

17 Factory certified and/or Branded computer system with i7/ Xenon or better processor, 32 GB RAM or better and 1 TB SSD & in-built 2 TB HDD or better, 22-23" or larger full HD Monitor with licensed latest windows compatible with equipment software, MS office latest standard version and antivirus for 3 years. System should have minimum 2 network ports. Printer- Coloured-duplex-legal, A4 with Network card and Bluetooth/Wi-Fi facility. One additional All-in-one computer with audio & video system, i5 or better, 16 GB RAM, 512 SSD & 1 TB HDD; 22-23" or larger full HD Monitor with licensed latest windows 11 Pro, MS office latest standard version and antivirus for 3 years - for report preparation. System should have minimum 2 network ports.

18 OEM should have application support laboratory and trained support team on the quoted model in India for local & efficient after sales service and application support.

19 Sanger sequencing consumables kit for 1000 reaction should be included.

20 IQ/OQ and certification after complete installation should be provided.

21 Comprehensive training in operation, handling, maintenance and data analysis should be provided by application specialists and trained engineers.

22 Trained technical manpower for 1 year for developing, demonstrating, validating and running the methods. The provided manpower shall be responsible for smooth running of the equipment.

System should be quoted with all accessories required to make it fully operational.

Total Amount of Part A

PART B - Optional Item

1 Sanger sequencing consumables kit for 1000 reaction.

Total FOR Amount upto Site - Part A + Part B

Total Amount in Words:

- It is mandatory to quote item wise rates for Part A lump sum rates are not acceptable for these.

- Bidder must quote amount in the given cells, if amount against any cell is included in main component, bidder should put "Zero" value.

- Taxes must be mentioned, if it is not mentioned, the same shall be treated as included in given rates.

- This is to certify that the rates quoted above are not more than the rates charged from any Institution/Department/Organization

Signatory Name: