Lumpsum Turnkey Contract on Design Build installation commissioning and operation of an Integrated Plant for the Production and Marketing of Agricultural Bio inputs Bio fertilizers and Bio pesticides for 5 Years at BNCA

Amount

Analytical cum microbiology lab (Supply & insttalation where necessary)

Analytical Balance Capacity: 220 g or higher.  Readability: Must be 0.001 g or better.  Repeatability: Must be ± 0.001 g or better.  Display: Must be LCD or better.  Linearity: Must be ± 0.02 g or better.  Pan size: Diameter must be 90 mm or higher.  Pan material: Must be made of stainless steel or similar material.  Interface: Must be standard RS 232C interface or better.  Must have options for auto power off and auto calibration.  Must have fast weighing response time.  Must be compact, space saving and reliable design.  Power: AC 230 V, + 10 %, 50-60 Hz.

Digital pH meter pH Range 0 to 14 pH Resolution 0.001 Stability 0.002 pH per hour Relative accuracy ± 0.002 pH ±1 digit Slope 85% to 115 % Standard buffer 1.679, 4.004,7.000, 9.183,12.454 acceptable Buffer deviation ± 0.5 pH Other working buffers 0 to 14 pH ( value entered numerical keys) mV Range 0 to ± 1999.9 mV Resolution 0.1 mV Accuracy ± 0.5 mV ± 1 digit or ± 0.05 % of reading Temperature Compensation Auto/manual Range 0 to 99.9 0 C Resolution 0.1 0 C Accuracy ± 0.5 0C ± 1 digit Display 2 lines, 20- character alphanumeric LCD Annunciation Individual LED with Keys for calibration, pH, ABS mV, REL mv and print Data storage 80 reading Printer port Epson committable 80 columns Dot matrix Power 230 V AC ± 10%, 50 Hz Dimensions 250 (W) x 205 (D) x 75 (H) mm Weight 1.25 kg ( Approx) Accessories i) pH electrode (combined) ii) Temperature probe (PT-100 sensor) iii) Electrode standard and clamp iv) Redox electrode (Pt. electrode) v) Calomel electrode vi) 80 column dot matrix printer

Hot air oven,91L  Capacity: 91 Litres or higher.  Usable Inner Dimension: 450 X 450 X450 mm or better.  Shelves: Minimum 2 Nos.  Heater power: 1.5 kW  Electric supply: 230 VAC, 50 Hz, single phase  Must have PID Control with timer.  Temperature range upto 250⁰C or higher.  Control Accuracy: ±0.5⁰C or better.  Uniformity: ±2.0 at 100.0⁰C or better.  Must have auto cut off off heater and fan on door open.  Must have toughened glass in main door for easy viewing of loads.  Must be of forced convection type.  Internal chamber must be of SS 304 or better and must have clear bottom and rounded corners for easy cleaning.  Must have stainless steel wire mesh trays.  Must have abrasion resistance and durable finish by heat cured epoxy coating on steel exterior.  Must have over temperature safety.  Must have long life industrial grade incoloy sheathed tubular heaters or better.

Laboratory refrigerator. Capacity: 300 Litres or higher.  Temperature range: 1oC – 10oC.  No. of doors (glass): Minimum 1 No.  No. of shelves: 4 or more.  Dimensions (mm): 584 x 584 x 1702 or better.

Magnetic stirrer cum hot plate Stirring capacity: 2 Litres or higher.  Heating capacity: 300 watts or higher.

Laboratory autoclave Capacity: 53 Litres or higher.  Inner Dimensions: Ø 350 X 550 mm.  Operating Parameters: 103.4 kPa (15psi)/121⁰C.  Heater: 2.25 kW.  Must have option for automatic purging for efficient sterilization.  Must have option for preset (at 121°C) Digital temperature indicator cum controller linked to a preset (20 min) timer.  Must have option for low water level cut off.  Must be fitted with a safety valve for added safety.  Must have end of cycle buzzer.  Must be supplied with traceable certificate for Pressure Gauge and temperature indicator.  Must have preset digital temperature indicator cum controller linked to a preset timer with 1⁰C resolution and ±1⁰C accuracy.  Must have Pt 100 Sensor for precise control and monitoring.  Must have drain valve on side at bottom for easy cleaning.  Must have SS 304 double walled construction.  Must be with SS wire mesh Carrier(s) and spare gasket.

Laboratory autoclave. Capacity: 113 Litres or higher.  Inner Dimensions: Ø 450 X 710 mm.  Operating Parameters: 103.4 kPa (15psi)/121⁰C.  Carrier: 2 of 400 x 330 mm.  Heater: 3.50 kW.  Must have option for automatic purging for efficient sterilization.  Must have option for preset (at 121°C) Digital temperature indicator cum controller linked to a preset (20 min) timer.  Must have option for low water level cut off.  Must be fitted with a safety valve for added safety.  Must have end of cycle buzzer.  Must be supplied with traceable certificate for Pressure Gauge and temperature indicator.  Must have preset digital temperature indicator cum controller linked to a preset timer with 1⁰C resolution and ±1⁰C accuracy.  Must have Pt 100 Sensor for precise control and monitoring.  Must have drain valve on side at bottom for easy cleaning.  Must have SS 304 double walled construction.  Must be with SS wire mesh Carrier(s) and spare gasket.

Laboratory autoclave  Capacity: 180 Litres or higher.  Inner Dimensions: Ø 550 X 760 mm.  Operating Parameters: 103.4 kPa (15psi)/121⁰C.  Heater: 5.00 kW.  Must have option for automatic purging for efficient sterilization.  Must have option for preset (at 121°C) Digital temperature indicator cum controller linked to a preset (20 min) timer.  Must have option for low water level cut off.  Must be fitted with a safety valve for added safety.  Must have end of cycle buzzer.  Must be supplied with traceable certificate for Pressure Gauge and temperature indicator.  Must have preset digital temperature indicator cum controller linked to a preset timer with 1⁰C resolution and ±1⁰C accuracy.  Must have Pt 100 Sensor for precise control and monitoring.  Must have drain valve on side at bottom for easy cleaning.  Must have SS 304 double walled construction.  Must be with SS wire mesh Carrier(s) and spare gasket.

Laminar air flow cabinet. Work area cleanliness ISO 5 Class 100 or better.  Work chamber size W x D x H: 90 x 60 x 60 cm ±1 cm tolerance or better.  External size W x D x H: 100 x 80 x 205 cm ± 3 cm tolerance or better.  Work table made of 1.5 mm thick SS 304 sheet or better.  Inner chamber made of SS 304 or better.  Outer body made of MS duly powder coated or better.  SS 304 Arm Rest or better.  Inner work area side walls and back walls made of single piece SS sheet with rounded corners to reduce contamination spot and for easy cleaning.  LED light for Illumination and UV germicidal light for disinfection.  Pre Filter 10 micron and HEPA filter of 0.3 micron.  HEPA filter range up to 10 mm to 35 mm WC.  Sash opening height indication upto 12” from table or better.  Gauge to monitor pressure drop inside the unit.  ISO 9001, ISO 14001, ISO 45001 and CE certified.  Test Certificate and document to be submitted at time of delivery - flow velocity, particle count test and PAO test, calibration report of gauge and operation manual.

Deep Freezer. Internal volume: 200 Litres or higher.  Minimum temperature: - 20oC or lower.  Insulation: Minimum 80 mm CFC free polyurethane foam for body and door.  Temperature control: Microprocessor based or better.  Must have visual alarm for power failure and audio door open alarm.  Internal body material: SS 304 or better.  External body material: Powder coated CRCA steel or better.  Noise level: 65 db or lower.

Bacteriological incubator. Capacity: 150 Litres (5.3 Cu. Ft.) or higher.  Inner Dimensions: 500 x 500 x 600 mm or higher.  Shelves: 2 Nos.  Must have PID Control with timer on outer door for easy access.  Control Accuracy: ±0.5°C or better.  Temperature range: +5 to 60°C or better.  Must have provision for easy viewing of samples without disturbing temperature.  Timer upto 9999 min. or better.  Must have abrasion resistance and durable finish by heat cured epoxy coating on steel exterior.  Must have long life industrial grade heaters.  Must have electric overload safety with MCB & Over temperature safety.  Must have inner chamber with rounded corners for easy cleaning & better performance.  Must have inner door frameless and made of tempered safety glass.  Must have adjustable shelves made of stainless steel wire mesh or better.  Must have inbuilt validation port to avoid gasket damage during validation.  Must have chamber illumination provision with fluorescent light(s).

Incubator 600L. Capacity: 600 Litres (21.3 Cu. Ft.) or higher.  Inner Dimensions: 700 x 700 x 1230 mm.  Must have PID Control with timer on outer door for easy access.  Control Accuracy: ±0.5°C or better.  Temperature range: +5 to 60°C or better.  Must have provision for easy viewing of samples without disturbing temperature.  Timer upto 9999 min. or better.  Must have abrasion resistance and durable finish by heat cured epoxy coating on steel exterior.  Must have long life industrial grade heaters.  Must have electric overload safety with MCB & Over temperature safety.  Must have inner chamber with rounded corners for easy cleaning & better performance.  Must have inner door frameless and made of tempered safety glass.  Must have adjustable shelves made of stainless steel wire mesh or better.  Must have inbuilt validation port to avoid gasket damage during validation.  Must have chamber illumination provision with fluorescent light(s).

Automatic colony counter. The system should have all stainless steel body.  Should have CMOS color camera with M12 lens and Zoom x28  Resolution should be 1 megapixel or higher.  Counting time: up to 1000 colonies per second or higher.  Minimum size of colony: 0.1 mm or better.  Lighting technology: long-life white LEDs / Dark Field.  Lighting system: Should have automatic 6 combinations  Should have top and/or bottom light, white or black background  Counting: automatic with manual control.  Counting Petri dishes of size Ø 55 - 90 mm or better.  Should have provision for counting on pour plating, surface plating, Spiral plating and circle plating.  Should have provision for automatic separation of clustered colonies  Should have provision for export of data to printed report, PDF, jpg, png, bmp and Excel etc.

Trinocular upright microscope with digital colour camera. Mandatory Specification 1: Interchangeable 30-Watt halogen bulb and energy-saving LED illumination white-light, 5600K, lifetime 35000 hours both should be available in the same stand, Eco Mode, Light Manager. Trinocular Observation & Documentation tube (50%:50%) with 25 degree inclination angle of observation and field of view 22 mm, with interpupillary distance continuously variable from 48 to 75 mm Mandatory Specification 2: 5-element display on either side of the stand and long-life blue LEDs. Color filter set blue, green, yellow. Light manager to remember precise amount of light set for each lens position. ECO mode, automatically go into a sleep mode after 15 min inactivity to improve Lamp Life. Mechanical rack less stage 75x50, drive right and specimen holder for double slides, with spring clip left. With power unit integrated into stand with cable storage at the backside of the stand. 5V USB port at the back of the stand to connect a power bank or charge mobile device. 6V 30W halogen lamp (one spare lamp). With Dust Cover Mandatory Specification 3: Anti-fungus treated optics: Anti-fungus certification from manufacturer. Suitable objective lenses Magnification 40x, to 1000x. Color-corrected infinity optics with High quality objectives of the iPlan-Achromat class: (4x/0.1, WD: 30.7 mm); (10x/0.25, WD: 17.4 mm) (40x/0.65, WD: 0.60 mm); (100x/1.25, Oil, WD 0.27mm, incl. Immersion oil, 5 ml).5-position nosepiece tilted back and objectives secured against unauthorized removal. Eyepiece: A pair of eyepieces 10x suitable for spectacle wearers and with field of view of 22mm. Condenser: Focusable and center able Abbe condenser 0.9/1.25 Mandatory Specification 4: Digital connectivity and documentation Camera: 6 Mega Pixel Camera Sensor: CMOS, Sensor size: 1/2.8ʺ Live Resolution 1920 x 1080, Frame speed: 1920 x 1080 30 fps via USB2.0 1920 x 1080 60 fps via HDMI Image Record: High Speed SD Card (8G) Video Record: 30 fps in PC , 30fps @SD Card 1080 Exposure time: 2ms – 10s White balance: Auto/Manual, Settings: Gain, Gamma, Saturation, Contraction Output model 1: USB2.0, Output model 2: HDMI System Compatible: XP. Win 7/8, 32/64 Bit, MAC Complete with HDMI Cable, USB Cable, Power adapter, SD Card, Mouse, IS Capture software . Microscope has to fulfil the standards: CE, CSA, UL, etc. Microscope and camera should be from same manufacturer for better compatibility. Schematic diagram of the Microscope to be provided. With branded computer for image analysis 1. Branded Desktop computer -i5 processor or equivalent latest version, 64 bits, 1 TB HDD, 4 GB RAM, 21-inch monitor, DVD Writer, Optical mouse, USB Key board, 1X PCL Express slot 2. USB 600A 3.HP/Canon/Epson colour Deskjet printer or higher

Incubator Shaker. Temperature range & accuracy: 5°C to 60°C, ± 0.5°C or better.  Internal Volume (Litres): 215 or higher.  Platform Size: 580mm x 600mm or higher.  Maximum Shaking Capacity (Volume x No. of flasks): 100ml x 49, 150ml x 49, 250ml x 33, 500ml x 24, 1000ml x 15, 2000 ml x 9 or more.  Shaking Speed range (RPM): 20 to 250 or higher.  Temperature control: Microprocessor with PT-100 sensor or better.  Must have audio visual alarm and audio alarm for power failure and door open respectively.  Illumination: Fluorescent Tube of capacity 8 Watts or higher.  Internal Body Material: SS 304 or better.  External Body Material: Powder coated CRCA steel or better.  Insulation: 70 mm and 80 mm CFC free polyurethane foam for body and door respectively.  Noise Level: 65 db or lower.  Suitable clamps for 250 ml, 500 ml, 1000 ml & 2000 ml flasks must be supplied with the system.

Weighing Scale Capacity: 10 kg or higher.  Accuracy: 1 g or higher.

Atomic absorption spectrometer. The Atomic Absorption Spectrometer must be true optically double beam and fully computer controlled.Dual Optics- Both Single Beam for high sensitivity and True Double Beam for long term stability mode in one system through one software click. Lamp turret with fully automatic alignment of 8 or more lamps. e instrument must be suitable to detect different elements which must also include arsenic. Hollow cathode lamp for Fe, Pb, Zn, Ni, Cd, Mn, Cu, Cr to be provided with the stem.The instrument must have fully sealed quartz quoted optics with a high-performance Czerny Turner monochromator with a flat holographic grating with 1800 lines / mm or better.  Wavelength range: 190 to 900 nm or better.  Wavelength accuracy and stability: ≤ ±0.04 nm or better.  UV Sensor for Flame Detection: UV sensors are only used for Hydrogen flame but due to risk involved of using combustible gases and its leak, to prevent accident if flame is not on- the gases are shut off.  Sensitivity of the instrument for flame operation must not be less than 0.9 Abs of 5 ppm Cu or better.  Automatic slit width: 0.2 to 1.2 nm or better.  Detector must be solid state detector or better.  Must have Titanium Burner heads for Nitrous Oxide-Acetylene flame. Nebulizer: Adjustable nebulizer with internal Pt/Rh capillary and acid-resistant ceramic impact bead.  Spray chamber: Must have PPS spray chamber with flow spoiler for aqueous and organic solutions.  Wavelength reproducibility: 0.005 nm or better.  Air compressor.  Latest software with upgrade option must be provided to fully control the instrument through PC.  Hydride system for As, Hg etc.: Computer Control electrically heated cell.  Optional Scrapper- Unique accessory in flame AAS, Motorized fully automatic cleaning of the burner head slit (removal of Carbon suite formation)  100 ml certified standard solutions of 1000 ppm concentration for all elements to be supplied.  Warranty of minimum 2 year must be provided along with 10 years warranty on the optics.  The bidder must quote for only those equipments for which hardware, software and spare parts support will be available in next minimum 10 years.  It must comply with international safety and quality standards (ISO and CE) and valid documents in support of this must be submitted.  The system must be supplied with following essential items: i. Suitable compatible branded i5 desktop computer along with compatible laser jet printer. ii. One acetylene filled cylinder (47 L) filled with gases, with necessary tubing, connectors and double stage gas regulator. iii. Complete set of stainless steel double fume hood with exhaust fan including necessary fitting and ducting facility. The system should be supplied with a fume hood as per following specifications:  Work chamber size: 3’ W x 2’ D x 3’ H feet or higher.  Work table of 3’ x 2’ made of Black Granite or SS 304.  Inner chamber made of SS 304.  Outer chamber made of MS duly powder coated.  External size: 48” W x 36” D x 84” H.  Stream lined airfoil made at the bottom of hood work table opening for smooth flow and avoiding turbulence inside the hood.  LED light fitted inside the chamber.  A sliding door made of 4mm thick glass fitted in aluminium frame moves vertically up/down using counter balanced weight mechanism.  Motor-blower exhaust system having 1 hp motor.  Storage cabinet below work chamber.  ISO 9001, ISO 14001.

Refregerated Centrifuge. Maximum speed: 20000 rpm or higher.  Maximum tube size: 100 ml or higher.  Maximum capacity: 400 ml or higher.  Lowest temperature: -8 oC or lower.  Noise: ≤ 60 dB or lower. The system should be supplied with compatible rotors for using 1.5 ml, 25 ml and 100 ml PP/PC tubes.

Bio-Fertilizer production & packaging line(Supply & insttalation where necessary)

Seed fermenter, Gross Volume: 50 Litres or higher• Type: Sterilization in Place (SIP) • H/D working volume: 2.5:1The fermenter should be ISO 9001:2015, ISO 14001:2015 and CE certified as per below technical specifications: • Vessel standard- The vessel should be designed as per ASME standards to comply with the requirements of the pressure vessel and bioprocess. • Working Volume- Up to 37.5L. • Vessel Φ : Φ295 X 735 X 4 mm • Jacket Φ : Φ335X 552 X 4 mm • Design pressure :3.75 kg/cm² at full vacuum • Design temp. :0 to 150° • Material of Construction (MOC): all contact parts are SS-316 & non-contact are SS-304 Thickness: • Vessel shell : S.S. 316 L ,thickness 4mm • Jacket shell : S.S. 304, thickness 4mm • Top Plate : S.S 316 L and thickness 20 mm • Air Sparger : S.S. 316L, ring type Top Port (S.S. 316 L) • Fermenter vessel should consist of SS316L Ports: inlet port, air inlet and outlet/ Vent port, pressure gauge port, inoculums port, one additional port, Light glass, for dosing acid, base, antifoam etc. SIDE Ports (S.S. 316L) • Fermenter vessel should have sparger port, Temperature sensor, pH sensor & sampling Valve. • Harvesting port in the bottom of the vessel. MOC : S.S. 316L, Size : 25mm Inner Surface: 240 grit finished & mirror polished Ra<0.8 micron M.O.C.: All contact parts should be SS-316 &Non-contact are SS-304 Gaskets and O’ rings: Silicon/EPDM material. Sight Glass and Light Glass Assembly : • Fermenter vessel should have sight/view glass assembly of size : 30 x 200mm, MOC-S.S. 316 L, glass thickness: 15 mm. Light Glass: Φ 45mmx15mm Provision of Ports- Should be provided on the top plate for safety valve, Agitator assembly, light glass, pressure gauge. An additional two nos. of ports have to be provided. Air sparger, 3 nos. of Standard ports for acid, Alkali and feed. At 1/3 height from bottom- 25 mm standard ports for pH, temperature probes and a sampling valve, In the bottom dish- Drain valve, in jacket-Steam/water entry- standard ports- 2nos, Jacket drain- standard – I no. • Mixing System/Agitator Assembly: SS316 L Stirrer Assembly with shaft, 2 smooth bearings, Height Adjustable Rushton turbine Impeller 3 no having 6 blades, single mechanical seal with 1HP AC motor, RPM: 50-400. • Impellers: Rushton turbine type standard impellers, 6 blades three impellers, removable type and made of SS316, • Motor:1-2HP, RPM :50-400 with VFD, AC motor with single mechanical seal • Sparger Type:- Ring type Sparger coil for aeration provided with fine pin holes of towards bottom of the vessel for uniform distribution of Air. • Inlet/Exhaust Filters:-0.22μm absolute Hydrophobic PTFE bacterial filter with SS housing. • Aeration Control/Air Flow control: Aeration from compressor, air pressure controlled automatically with Glass Metered Needle Valve Rotamter 0 to 50 LPM and fitted with 0.22μ Inlet filter air filter with airflow pressure gauge with safety valve. The filter is PTFE, Hydrophobic and Autoclavable Type. Consists of Inlet and outlet filter: 0.2 Micron PTFE, cartridge, 2.5 inch with SS 316 Housings, In- situ sterilizable. • MOC of Condenser:-Shell in Tube Type SS 316L Exhaust Condenser fitted on the top of the flange with 0.22 micron PTFE filter for out gas and air filtration. Water inlet and out let for circulation of cold water to maintain the reaction volume or cool down the vapors going out with exhaust gases. • Baffles:-SS316L 4 Nos. Baffles of width 1/10 of ID covering bottom to working volume for breaking the vortex for proper mixing. Baffles are removable Type. • Drain valve: 1” Diaphragm valve • Lantern & Bearing housing assembly: Housing with two bearings • No. of legs: 3/4, SS304. Sterilization Control: Control valves to control sterilization of fermenter having TFT/PLC Touch Screen control panel with display screen for media sterilization system. Fully automatic, range: 0 to 150°C with steam i.e. sterilizable in place (SIP) as per ASME standards. Automatic Temperature Controller: TFT/PLC controlled temperature monitoring & and Automatic control system, RTD temp. sensor (Pt.-100),19mm with SS pocket and port, Digital Temperature Indicator cum controller. Range: 0 to 150°C, measurement & Control precision: ± 0.1°C. • Heating Assembly: SS-304 Heat Exchanger with Steam Coil/heater. • Automatic RPM Controller: Variable RPM control within 50 to 400RPM with VFD. Auto control of temperature and variable RPM of agitator by providing four solenoid valve with variable frequency drive with control panel. • pH Measurement & Control: Supplied with a Gel filled type pH Sensor/Probe with cable, Measuring Range: 0 to14, Control Accuracy: ±0.1pH. (Make: Broadly James USA/ equivalent).Provided with 2 Nos. of Peristaltic pumps (fixed speed) for addition of Acid/ Base with suitable silicon tubing for acid/alkali Addition into the vessel. The system having Auto pH Calibration with 2 point or 3 points calibration i.e. either using 2 Buffers or 3 buffers. • Foam/ Level Controller: SS316L Conduction probes for automatic Level Foam control. Time Proportionate Control. Provided with one number peristaltic pump with antifoam sensor and cable. • Peristaltic Pump: Total 3 Nos. of Peristaltic pumps (for addition of Acid/ Base and Feed with suitable silicon tubing for acid/alkali addition into the vessel. The system having Auto pH Calibration by using Buffers solutions. • Accessories: System would be provided with 3 No. standard Borosilicate acid/base addition bottles with PTFE filters of 25 mm diameter, silicone tubing-3x6 and 6x9mm of 5 meter each, SS clamps etc. • Power Supply: 230V/ 50Hz, AC.

Production fermenter, Gross Volume: 500 Litres or higher. • Type: Sterilization in Place (SIP) • H/D working volume: 2.5:1 The fermenter should be ISO 9001:2015, ISO 14001:2015 and CE certified as per below technical specifications: • Vessel standard- The vessel should be designed as per ASME standards to comply with the requirements of the pressure vessel and bioprocess. • Working Volume- Up to 375L. • Vessel Φ : Φ635 X 1585 X 5 mm • Jacket Φ : Φ725X 1268 X 4 mm • Design pressure :3.75 kg/cm² at full vacuum • Design temp. :0 to 150°C • Material of Construction (MOC): all contact parts are SS-316 & non-contact are SS-304 Thickness: • Vessel shell : S.S. 316 L ,thickness 4mm • Jacket shell : S.S. 304, thickness 4mm • Top Plate : S.S 316 L and thickness 20 mm • Air Sparger : S.S. 316L, ring type Top Port (S.S. 316 L ½” to 1”) • Fermenter vessel should consist of SS316L Ports: inlet port, condensate, outlet port, in vessel and one in jacket, inoculums port, one additional port. Fermenter vessel should consist of multi-channel Ports for dozing of chemicals: Acid, alkali port, addition port and air out let port. SIDE Ports (S.S. 316L) • Fermenter vessel to have sparger port, Temperature sensor, pH, DO sensor & sampling Valve and harvesting port in the bottom of the vessel. MOC : S.S. 316L, Size : 25mm Inner Surface: 240 grit finished & mirror polished Ra<0.8 micron Gaskets and O’ rings: Silicon/EPDM material. Dish and shell thickness: 4 mm. Sight Glass and Light Glass Assembly : • Fermenter vessel having sight/view glass assembly of size : 30 x 300mm, MOC-S.S. 316 L, glass thickness: 15 mm. Light Glass: Φ 65mm x 15mm. Provision of Ports- Provided on the top plate for safety valve, Agitator assembly, light glass, pressure gauge. An additional two nos. of ports have provided. Air sparger, 3 nos. of Standard ports for acid, Alkali and feed. At 1/3 height from bottom- 25 mm standard ports for pH, temperature probes and a sampling valve, In the bottom dish- Drain valve, in jacket-Steam/water entry- standard ports- 2nos, Jacket drain- standard – I no. • Mixing System/Agitator Assembly: SS316 L Stirrer Assembly with shaft, 2 smooth bearings, Height Adjustable Rushton turbine Impeller 3 no having 6 blades, single mechanical seal with 1HP AC motor, RPM: 50-400. • Impellers: Rushton turbine type standard impellers, 4 blades (or more), three impellers, removable type and made of SS316, • Motor:3-5HP, RPM :50-300 with VFD, AC motor with single mechanical seal • Sparger Type:- Ring type 25 mm Sparger coil for aeration provided with fine pin holes of towards bottom of the vessel for uniform distribution of Air. • Inlet/Exhaust Filters:-0.22μm absolute Hydrophobic PTFE bacterial filter with SS housing. • Aeration Control/Air Flow control: Aeration from compressor, air pressure controlled automatically with Glass Metered Needle Valve Rotameter 0 to 50 LPM and fitted with 0.22μ Inlet filter air filter with airflow pressure gauge with safety valve. The filter is PTFE, Hydrophobic and Autoclavable Type 2.5 inch with SS 316 Housings, In- situ sterilizable. • MOC of Condenser:-Shell in Tube Type SS 316L Exhaust Condenser fitted on the top of the flange with 0.22 micron PTFE filter for out gas and air filtration. Water inlet and out let for circulation of cold water to maintain the reaction volume or cool down the vapors going out with exhaust gases. • Baffles:-SS316L 4 Nos. Baffles provided for breaking the vortex for proper mixing. Baffles are removable Type. • Drain valve: 1” Diaphragm valve • Lantern & Bearing housing assembly: Housing with two bearings • No. of legs: 3/4, SS304. Sterilization Control: Control valves to control sterilization of fermenter having TFT/PLC Touch Screen control panel with display screen for media sterilization system. Fully automatic, range: 0 to 150°C with steam i.e. sterilizable in place (SIP) as per ASME standards. Automatic Temperature Controller: TFT/PLC controlled temperature monitoring & and Automatic control system, RTD temp. sensor (Pt.-100),19mm with SS pocket and port, Digital Temperature Indicator cum controller. Range: 0 to 150°C, measurement & Control precision: ± 0.1°C. • Heating Assembly: SS-304 Heat Exchanger with Steam Coil/heater. • Automatic RPM Controller: Variable RPM control within 50 to 300RPM. Auto control of temperature and variable RPM of agitator by providing four solenoid valve with variable frequency drive with control panel. • pH Measurement & Control: Supplied with a Gel filled type pH Sensor/Probe with cable, Measuring Range: 0 to14, Control Accuracy: ±0.1pH. (Make: Broadly James USA/ equivalent).Provided with 2 Nos. of Peristaltic pumps (fixed speed) for addition of Acid/ Base with suitable silicon tubing for acid/alkali Addition into the vessel. The system having Auto pH Calibration with 2 point or 3 points calibration i.e. either using 2 Buffers or 3 buffers. • Foam/ Level Controller: SS316L Conduction probes for automatic Level Foam control. Time Proportionate Control. Provided with one number peristaltic pump with antifoam sensor and cable. • Peristaltic Pump: Total 3 Nos. of Peristaltic pumps (for addition of Acid/ Base and Feed with suitable silicon tubing for acid/alkali addition into the vessel. The system having Auto pH Calibration by using Buffers solutions. • Accessories: System would be provided with 3 No. standard Borosilicate acid/base addition bottles with PTFE filters of 25 mm diameter, silicone tubing-3x6 and 6x9mm of 5 meter each, SS clamps etc. • Power Supply: 230V/ 50Hz, AC.

Media Preparation vessel.500L with associated aseptic pipeling works.

Combined control panel Automation and control • The system should consist of a bright & multi color TFT/PLC panel (common for seed and production fermenters) to manage fermenter, boiler, chiller and air compressors from 5.7” TFT Touch screen panel. • Should also have USB port/interface to transfer the data to computer through software.

Steam generator, Steam output: 120 kg/hr or higher.  Maximum Working Pressure: 4-5 kg/cm2 or higher pressure.  Fuel: Diesel oil.

Chiller circulator, Skid mounted air cooled chiller of suitable capacity for the entire bio-fertilizer production line..

Air Compressor  Working pressure: 4 kg/cm2 or better.  Capacity at full load pressure: 40 CFM or better.  Motor rating: 2 HP or better.  Motor voltage: 230 V AC.

Cooling Tower,70 TR with circulation pumps.

Sanitary Aseptic transfer lines.

Pipelines, valves & fittings:Piping, tubing, valves, pressure gauge & safety valve for process operations. Piping size (1/2” line), steam trap size (1/2” line), pneumatic angel valves size (3/4”), air flow regulator and air (3/4”line with Filter housing 3/4”).  Diaphragm valves (1/2” line), ball valves (1/2”), and relief valves size (1/2”).  Pressure gauges type (0-7Kg/cm2) , Rating : 0 to 7 kg/cm²  Top condenser type: Shell & tube type (SS316L), vertical with cold water circulation inlet/ outlet through, S.S.304 jacket, top condenser SS316L.  SS304 pipeline fittings, ball valves, Non Return valves, circulation Pump and Relief valve.  Heat exchanger, airflow control, Housing Filters, silicone tubing, SS clamps etc.

Common clean in place (CIP) system: Should have common Clean in place (CIP) system comprising of 200 Litres tank fitted with a centrifugal pump of ½ HP and cleaning spray balls on a skid with lockable wheel (common for seed and production fermenters.

Culture mixing/holding vessel Gross Volume: 500 Litres.  MOC: SS 316L for wetted parts & SS 304 for non-wetted parts.  The vessel should have provision for sterilization, ports for addition of additives, stirring/mixing of cultures or any extra parameter deemed to be suitable for mixing of cultures.  Should have connecting aseptic pipelines for transferring the cultures to bottling line.

Automated liquid filling & capping (under laminar air flow cabinet), sealing and labelling machine: The liquid filling/bottling machine should be an automatic four head piston filling machine with provision for filling bottles of capacity 500 ml to 2500 ml. The estimated bottling speed should be atleast 500 bottles/hour of 1000 ml bottle capacity.  The turn table, liquid filling area and capping must be under laminar air flow cabinet to minimize the risk of contamination.  The system should have automatic screw capping of SS body.  The system should be accompanied with an induction sealing machine and bottle labelling machine.

Screw capping machine:Automatic,versatile,self supported.No container no cap arrangement.Magnetic Antilock cap & pressing.standard make motor.1 HP single Ph synchronizes conveyor,star wheel & platform,adjustable height of conveyor belt,SS 304 make,torque adjustable,conveyoyor length:6 ft.High speed cap feeder system.

Induction sealing machine:Latest IGBT ,a neck sze upoto 38 mm is suitable to sal 70 to 80 bottle s per minute,& for aneck range upto 50 mm for 40 to 50 bottles per minute,control panel-250V AC 50Hz,input current:7Amp,output power:2 KW,1/8 HP water pump,flow switch,.induction unit- consoisting epoxy 7 capsulated induction head, output transformer unit at adjustable,cables & installation assesories,conveyor specification-L: 4 ft, standard ht.850 mm x 1200 mm,SS made conveyor,slate chain all access part.,1/4 HP A.C motor & gear box

Bottle Labelling machine: capable of labelling upto 60 containers per minute.,Technical specification:Direction of Movement left to right.,output-50 BPM,Conveyor Motor rotomorive,Ht. Conveyor-860 mm to 910 mm

Clean room/chamber(As directed by employer)

Bio-Pesticide production & packaging line(Supply & insttalation where necessary)

Seed fermenter,. Gross Volume: 50 Litres or higher. • Type: Sterilization in Place (SIP) • H/D working volume: 2.5:1 The fermenter should be ISO 9001:2015, ISO 14001:2015 and CE certified as per below technical specifications Vessel standard- The vessel should be designed as per ASME standards to comply with the requirements of the pressure vessel and bioprocess. • Working Volume- Up to 37.5L. • Vessel Φ : Φ295 X 735 X 4 mm • Jacket Φ : Φ335X 552 X 4 mm • Design pressure :3.75 kg/cm² at full vacuum • Design temp. :0 to 150° • Material of Construction (MOC): all contact parts are SS-316 & non-contact are SS-304 Thickness: • Vessel shell : S.S. 316 L ,thickness 4mm • Jacket shell : S.S. 304, thickness 4mm • Top Plate : S.S 316 L and thickness 20 mm • Air Sparger : S.S. 316L, ring type Top Port (S.S. 316 L) • Fermenter vessel should consist of SS316L Ports: inlet port, air inlet and outlet/ Vent port, pressure gauge port, inoculums port, one additional port, Light glass, for dosing acid, base, antifoam etc. SIDE Ports (S.S. 316L) • Fermenter vessel should have sparger port, Temperature sensor, pH sensor & sampling Valve. • Harvesting port in the bottom of the vessel. MOC : S.S. 316L, Size : 25mm Inner Surface: 240 grit finished & mirror polished Ra<0.8 micron M.O.C.: All contact parts should be SS-316 &Non-contact are SS-304 Gaskets and O’ rings: Silicon/EPDM material. Sight Glass and Light Glass Assembly : • Fermenter vessel should have sight/view glass assembly of size : 30 x 200mm, MOC-S.S. 316 L, glass thickness: 15 mm. Light Glass: Φ 45mmx15mm Provision of Ports- Should be provided on the top plate for safety valve, Agitator assembly, light glass, pressure gauge. An additional two nos. of ports have to be provided. Air sparger, 3 nos. of Standard ports for acid, Alkali and feed. At 1/3 height from bottom- 25 mm standard ports for pH, temperature probes and a sampling valve, In the bottom dish- Drain valve, in jacket-Steam/water entry- standard ports- 2nos, Jacket drain- standard – I no. • Mixing System/Agitator Assembly: SS316 L Stirrer Assembly with shaft, 2 smooth bearings, Height Adjustable Rushton turbine Impeller 3 no having 6 blades, single mechanical seal with 1HP AC motor, RPM: 50-400. • Impellers: Rushton turbine type standard impellers, 6 blades three impellers, removable type and made of SS316, • Motor:1-2HP, RPM :50-400 with VFD, AC motor with single mechanical seal • Sparger Type:- Ring type Sparger coil for aeration provided with fine pin holes of towards bottom of the vessel for uniform distribution of Air. • Inlet/Exhaust Filters:-0.22μm absolute Hydrophobic PTFE bacterial filter with SS housing. • Aeration Control/Air Flow control: Aeration from compressor, air pressure controlled automatically with Glass Metered Needle Valve Rotamter 0 to 50 LPM and fitted with 0.22μ Inlet filter air filter with airflow pressure gauge with safety valve. The filter is PTFE, Hydrophobic and Autoclavable Type. Consists of Inlet and outlet filter: 0.2 Micron PTFE, cartridge, 2.5 inch with SS 316 Housings, In- situ sterilizable. • MOC of Condenser:-Shell in Tube Type SS 316L Exhaust Condenser fitted on the top of the flange with 0.22 micron PTFE filter for out gas and air filtration. Water inlet and out let for circulation of cold water to maintain the reaction volume or cool down the vapors going out with exhaust gases. • Baffles:-SS316L 4 Nos. Baffles of width 1/10 of ID covering bottom to working volume for breaking the vortex for proper mixing. Baffles are removable Type. • Drain valve: 1” Diaphragm valve • Lantern & Bearing housing assembly: Housing with two bearings • No. of legs: 3/4, SS304. Sterilization Control: Control valves to control sterilization of fermenter having TFT/PLC Touch Screen control panel with display screen for media sterilization system. Fully automatic, range: 0 to 150°C with steam i.e. sterilizable in place (SIP) as per ASME standards. Automatic Temperature Controller: TFT/PLC controlled temperature monitoring & and Automatic control system, RTD temp. sensor (Pt.-100),19mm with SS pocket and port, Digital Temperature Indicator cum controller. Range: 0 to 150°C, measurement & Control precision: ± 0.1°C. • Heating Assembly: SS-304 Heat Exchanger with Steam Coil/heater. • Automatic RPM Controller: Variable RPM control within 50 to 400RPM with VFD. Auto control of temperature and variable RPM of agitator by providing four solenoid valve with variable frequency drive with control panel. • pH Measurement & Control: Supplied with a Gel filled type pH Sensor/Probe with cable, Measuring Range: 0 to14, Control Accuracy: ±0.1pH. (Make: Broadly James USA/ equivalent).Provided with 2 Nos. of Peristaltic pumps (fixed speed) for addition of Acid/ Base with suitable silicon tubing for acid/alkali Addition into the vessel. The system having Auto pH Calibration with 2 point or 3 points calibration i.e. either using 2 Buffers or 3 buffers. • Foam/ Level Controller: SS316L Conduction probes for automatic Level Foam control. Time Proportionate Control. Provided with one number peristaltic pump with antifoam sensor and cable. • Peristaltic Pump: Total 3 Nos. of Peristaltic pumps (for addition of Acid/ Base and Feed with suitable silicon tubing for acid/alkali addition into the vessel. The system having Auto pH Calibration by using Buffers solutions. • Accessories: System would be provided with 3 No. standard Borosilicate acid/base addition bottles with PTFE filters of 25 mm diameter, silicone tubing-3x6 and 6x9mm of 5 meter each, SS clamps etc. • Power Supply: 230V/ 50Hz, AC.

Production fermenter,  Gross Volume: 50 Litres or higher. • Type: Sterilization in Place (SIP) • H/D working volume: 2.5:1 The fermenter should be ISO 9001:2015, ISO 14001:2015 and CE certified as per below technical specifications: • Vessel standard- The vessel should be designed as per ASME standards to comply with the requirements of the pressure vessel and bioprocess. • Working Volume- Up to 37.5L. • Vessel Φ : Φ295 X 735 X 4 mm • Jacket Φ : Φ335X 552 X 4 mm • Design pressure :3.75 kg/cm² at full vacuum • Design temp. :0 to 150° • Material of Construction (MOC): all contact parts are SS-316 & non-contact are SS-304 Thickness: • Vessel shell : S.S. 316 L ,thickness 4mm • Jacket shell : S.S. 304, thickness 4mm • Top Plate : S.S 316 L and thickness 20 mm • Air Sparger : S.S. 316L, ring type Top Port (S.S. 316 L) • Fermenter vessel should consist of SS316L Ports: inlet port, air inlet and outlet/ Vent port, pressure gauge port, inoculums port, one additional port, Light glass, for dosing acid, base, antifoam etc. SIDE Ports (S.S. 316L) • Fermenter vessel should have sparger port, Temperature sensor, pH sensor & sampling Valve. • Harvesting port in the bottom of the vessel. MOC : S.S. 316L, Size : 25mm Inner Surface: 240 grit finished & mirror polished Ra<0.8 micron M.O.C.: All contact parts should be SS-316 &Non-contact are SS-304 Gaskets and O’ rings: Silicon/EPDM material. Sight Glass and Light Glass Assembly : • Fermenter vessel should have sight/view glass assembly of size : 30 x 200mm, MOC-S.S. 316 L, glass thickness: 15 mm. Light Glass: Φ 45mmx15mm Provision of Ports- Should be provided on the top plate for safety valve, Agitator assembly, light glass, pressure gauge. An additional two nos. of ports have to be provided. Air sparger, 3 nos. of Standard ports for acid, Alkali and feed. At 1/3 height from bottom- 25 mm standard ports for pH, temperature probes and a sampling valve, In the bottom dish- Drain valve, in jacket-Steam/water entry- standard ports- 2nos, Jacket drain- standard – I no. • Mixing System/Agitator Assembly: SS316 L Stirrer Assembly with shaft, 2 smooth bearings, Height Adjustable Rushton turbine Impeller 3 no having 6 blades, single mechanical seal with 1HP AC motor, RPM: 50-400. • Impellers: Rushton turbine type standard impellers, 6 blades three impellers, removable type and made of SS316, • Motor:1-2HP, RPM :50-400 with VFD, AC motor with single mechanical seal • Sparger Type:- Ring type Sparger coil for aeration provided with fine pin holes of towards bottom of the vessel for uniform distribution of Air. • Inlet/Exhaust Filters:-0.22μm absolute Hydrophobic PTFE bacterial filter with SS housing. • Aeration Control/Air Flow control: Aeration from compressor, air pressure controlled automatically with Glass Metered Needle Valve Rotamter 0 to 50 LPM and fitted with 0.22μ Inlet filter air filter with airflow pressure gauge with safety valve. The filter is PTFE, Hydrophobic and Autoclavable Type. Consists of Inlet and outlet filter: 0.2 Micron PTFE, cartridge, 2.5 inch with SS 316 Housings, In- situ sterilizable. • MOC of Condenser:-Shell in Tube Type SS 316L Exhaust Condenser fitted on the top of the flange with 0.22 micron PTFE filter for out gas and air filtration. Water inlet and out let for circulation of cold water to maintain the reaction volume or cool down the vapors going out with exhaust gases. • Baffles:-SS316L 4 Nos. Baffles of width 1/10 of ID covering bottom to working volume for breaking the vortex for proper mixing. Baffles are removable Type. • Drain valve: 1” Diaphragm valve • Lantern & Bearing housing assembly: Housing with two bearings • No. of legs: 3/4, SS304. Sterilization Control: Control valves to control sterilization of fermenter having TFT/PLC Touch Screen control panel with display screen for media sterilization system. Fully automatic, range: 0 to 150°C with steam i.e. sterilizable in place (SIP) as per ASME standards. Automatic Temperature Controller: TFT/PLC controlled temperature monitoring & and Automatic control system, RTD temp. sensor (Pt.-100),19mm with SS pocket and port, Digital Temperature Indicator cum controller. Range: 0 to 150°C, measurement & Control precision: ± 0.1°C. • Heating Assembly: SS-304 Heat Exchanger with Steam Coil/heater. • Automatic RPM Controller: Variable RPM control within 50 to 400RPM with VFD. Auto control of temperature and variable RPM of agitator by providing four solenoid valve with variable frequency drive with control panel. • pH Measurement & Control: Supplied with a Gel filled type pH Sensor/Probe with cable, Measuring Range: 0 to14, Control Accuracy: ±0.1pH. (Make: Broadly James USA/ equivalent).Provided with 2 Nos. of Peristaltic pumps (fixed speed) for addition of Acid/ Base with suitable silicon tubing for acid/alkali Addition into the vessel. The system having Auto pH Calibration with 2 point or 3 points calibration i.e. either using 2 Buffers or 3 buffers. • Foam/ Level Controller: SS316L Conduction probes for automatic Level Foam control. Time Proportionate Control. Provided with one number peristaltic pump with antifoam sensor and cable. • Peristaltic Pump: Total 3 Nos. of Peristaltic pumps (for addition of Acid/ Base and Feed with suitable silicon tubing for acid/alkali addition into the vessel. The system having Auto pH Calibration by using Buffers solutions. • Accessories: System would be provided with 3 No. standard Borosilicate acid/base addition bottles with PTFE filters of 25 mm diameter, silicone tubing-3x6 and 6x9mm of 5 meter each, SS clamps etc. • Power Supply: 230V/ 50Hz, AC.

Control panel: Automation and control • The system should consist of a bright & multi color TFT/PLC panel (common for seed and production fermenters) to manage fermenter, boiler, chiller and air compressors from 5.7” TFT Touch screen panel. • Should also have USB port/interface to transfer the data to computer through software.

Air Compressor  Standard air compressor of suitable capacity to be provided for the bio-pesticide production line.

Chiller circulator, Standard chiller of suitable capacity to be provided for the bio-pesticide production line.

SIP ribbon mixer, Capacity: 50 kg or higher.  Type: Sterilizable in place (SIP)

Common cleaning in place (CIP) system: Should have common Clean in place (CIP) system comprising of 50 Litres tank fitted with a centrifugal pump of ½ HP and cleaning spray balls on a skid with lockable wheel (common for seed and production fermenters.

Semi automated liquid filling & capping (under laminar air flow cabinet),bottle and packet sealing machine: The liquid filling/bottling machine should be a semi-automatic piston filling machine with provision for filling bottles of capacity 250 ml to 2500 ml. The estimated bottling speed should be atleast200 bottles/hour of 1000 ml bottle capacity.  The turn table, liquid filling area and capping must be under laminar air flow cabinet to minimize the risk of contamination.  The system should be provided with asuitable screw capping machine.  The system should also be accompanied with an induction sealing machine for bottles.  A suitable pedal operated sealing machine for solid bio-pesticides must be provided.

Infrastructure(Supply & insttalation where necessary)

Civil infrastructure (14762 sq. ft.) G+ 1 RCC structure with rooftop guest house as per drawing 1. Soil Report (Borehole test) to be submitted along with the tender from Soil Testing Agency with approval from Project Manager. 2. Submission schematic design along with Tender Schematic Design: a. Floor Plans b. Typical Sections c. Outline Elevations d. Preliminary site layout plan e. Preliminary electrical and plumbing layout 3. Construction Programme 4. QA/QC Plan 5.HSE Plan 6.Construction Methodology 7. Resource Deployment Scheme- Manpower and Equipment

Water Treatment plant 2000LPH. Water treatment plant of suitable capacity for the entire building must be provided( Satisfaction of Project Manager)

RO water purification system, 500LPH. RO based water purification plant of suitable capacity for operation of both production lines must be provided( Satisfaction of Project Manager)

Overhead Water tanks ,3000L. Overhead water tanks (PE/PP) of suitable capacity for the entire building must be provided.( Satisfaction of Project Manager)

PC with printer. Any branded PC with b/w / colourprinter must be provided( Satisfaction of Project Manager)

7.5KVA Genset with shed. Automatic silent Diesel generator set of suitable capacity must be provided.

Furniture as per RFB. Office table: 5 Nos- 138cmx76.20cmx72.8cm with three drawer(Godrej Make)  Office revolving chair (with cushion): 15 Nos.(Godrej HB with head rest, 45-54 H x30Wx30D)  Godrej Make)  Almirah Full size: 3 Nos- 198cm(H)x91cm(W)x48.6cm(D)( Godrej Make)  Revolving stool: 15 Nos.( Godrej Make)  Laboratory work benches: 12 Nos.  Laboratory work benches (with wash basin): 5 Nos.  Reagent rack: 2 Nos.  Wall cabinets: 10 Nos( Wooden, 2.50m x 3.35m Wooden, Godrej Make)  16 seater conference table: 1 No.( Godrej Make)  Mid back revolving chair: 16 Nos( Godrej HB with head rest, 45-54 H x30Wx30D)  25 Nos- Revolving Chair- 105.5cm(H)x76cm(W)x76cm(D)( Godrej Make)  Single bed: 14 Nos-Wooden Godrej Make  Table with chair: 8 Seats  Air conditioner( 5 Star Non deducted Split Type, Capacity- 2 T-3 nos, , 5 Star Non deducted Split Type, Capacity- 1.5 T-2 Nos  65” LED Television( Ultra HD 4K: 1 No( Sony/LG/Samsung Make)  Centre table( 1000mmx750mm, Wooden Godrej Make): 1 No.  Sofa (5 Seater, Godrej Make ): 1 Set.  8 seater dining table with chairs( Wooden Godrej Make): 1 No.  Wall cabinet (Godrej Make - for kitchen): 3 Nos.  Kitchen/household utilities: As per requirement

Online UPS with SMF batteries 30 kVA. Online UPS of 15 kVA or higher with suitable SMF batteries and accessories must be provided.

Transportation

Light commercial vehicle ( SUMO VICTA( AC) /BOLERO MAHINDRA_AC). Should be a suitable pickup vehicle for transportation of the manufactured agricultural bio-inputs.  Overall dimensions: 4925 x 1700 x 1825 mm or better.  Load body: 2500 x 1620 x 458 mm or better.  Transmission: Manual.  Preferred engine capacity: 2500 cc or better.  Fuel: Diesel.