Chemicals

T4 DNA ligase Quantity 01 Size 2000units T4 DNA ligase is an enzyme derived from bacteriophage T4 that catalyzes the formation of phosphodiester bonds between adjacent 3′-OH and 5′-phosphate ends of DNA. It is widely used in molecular biology to join DNA fragments, such as ligating insert DNA into vectors during cloning. The enzyme requires ATP as a cofactor and functions efficiently on both cohesive (sticky) and blunt-ended DNA fragments.

BsaI Quantity 01 Size 1000units BsaI is a Type IIS restriction endonuclease that recognizes the asymmetric DNA sequence 5′-GGTCTC-3′ but cuts DNA at a defined distance outside of its recognition site. This property allows it to generate custom overhangs, making it widely used in Golden Gate cloning for seamless and directional assembly of DNA fragments. It requires Mg²⁺ as a cofactor and functions optimally under specific buffer and temperature conditions (typically 37 °C).

Acetosyringone Quantity 01 Size 5G Acetosyringone is a phenolic compound used in plant transformation protocols, particularly with Agrobacterium tumefaciens. It induces the expression of virulence (vir) genes in the bacterium, which are essential for T-DNA transfer into plant cells. It is commonly added to infection and co-cultivation media to enhance transformation efficiency and is typically dissolved in solvents like ethanol or DMSO for preparation.

Sodium hydroxide pellets Quantity 01 Size 500Gl Sodium hydroxide pellets are a strong alkaline reagent used in molecular biology for pH adjustment and cell lysis during DNA extraction protocols. They help denature DNA and proteins under alkaline conditions, as in alkaline lysis methods for plasmid isolation. NaOH is highly caustic and hygroscopic, so it must be handled with care and stored in tightly sealed containers to prevent moisture absorption.

5. CTAB Quantity 01 Size 100Gl CTAB is a cationic detergent widely used in plant DNA extraction protocols. It disrupts cell membranes and forms complexes with polysaccharides, allowing their separation from DNA. CTAB helps obtain high-quality DNA by removing contaminants such as polysaccharides and some proteins, making it especially useful for plant tissues rich in secondary metabolites.