Supply and installation of Single-molecule Localization Super-resolution Microscope.

Indian Institute of Science Education and Research Bhopal

Annexure-I(b) Annexure-I(b)

Technical Compliance Sheet

Tender No.: IISERB/SP/2025-26/109 Dated 27.02.2026

Item: Supply & installation of Single-molecule Localization Super-resolution Microscope.

Sl. No. Specifications Make and offered Model Authorization certificate attached/Not attached Comply / Not Comply

1 Resolution Single-molecule localization microscopy (SMLM) with 20 nm in XY (incl. STORM, DNA-PAINT, PALM) Widefield/TIRF

2 Illumination Control Option to select illumination angle: 0°-65°, Epifluorescence/widefield, HILO, TIRF, LED array for bright-field imaging Flat-field homogeneous laser illumination: coefficient of variation <22%

3 Lasers SMLM in multiple colors (4 lasers) and 2 simultaneous channels (dichroic mirror split at 640 nm)

4 Power At sample: 405 nm (15 mW), 488 nm (250 mW), 561 nm (250 mW), 640 nm (250 mW) At source: 405 nm (1 W), 488 nm (1 W), 561 nm (750 mW), 640 nm (1 W)

5 Switch on time 405 nm (1 ms), 488 nm (1 ms), 561 nm (3 ms), 640 nm (1 ms)

6 Power Density 4 kW/cm2 (0.24 kW/cm2 for UV), Continuous laser

7 Temperature Control Running temp 30-37°C, Sample temperature stable to +/- 0.1°C at steady state, +/- 0.2°C with typical light program engaged, +/- 0.3°C with 100% laser power, Precise temperature control with top-stage incubator using resistive heating as heating elements keep the entire unit at a uniform temperature for live cell imaging and single-particle tracking

8 Focus Z-lock Controllable Z offset +/- 10 µm from coverslip interface, Rapid and precise stabilization of microscope focus, with locking technology to minimize Z-drift, LED for bright-field imaging, NIR auto-focus laser

9 Channel Mapping Sub-pixel alignment between optical channels

10 Mechanical Stability <1 µm/K drift

11 Imaging Technologies 2D and 3D single-molecule localization microscopy (SMLM), dSTORM, PALM, DNA-PAINT, Single-particle tracking (SPT), Total internal reflection fluorescence (TIRF), Single-molecule Förster resonance energy transfer (smFRET)

12 Camera Hamamatsu Orca Flash4.0 v3 Frame rate Full FOV: up to 10 ms / 100 Hz Cropped FOV: up to 1 ms / 1000 Hz

13 Objectives & Field of View (FOV) Objective compatibility, Standard: 100X NA 1.45 FOV with 100X oil objective with WD 0.13 mm: 80 μm x 50 μm, 3D imaging technique

14 Single-molecule Localization kit LNP profiler Kit (Quantity 5), EV profiler Kit (Quantity 5), DNA paint kit (Quantity 10), dSTROM discovery kit (Quantity 10), dSTROM training kit (Quantity 10). Imaging buffer for dSTROM live-cell imaging.

15 Software Data analysis Software with unlimited user access

16 Computer Suitable computer/workstation (a minimum Core i-9, 14 generation, preferably 8 TB SSD-Generation 4, preferably 512 GB DDR5 RAM, preferably 16 GB graphics NVIDIA card, with compatible accessories) with all the interfacing hardware and pre-loaded software to operate the system with full capabilities and the license key must be provided. Additional computer/workstation with above specification for remote data analysis must be provided. Provide software should be compatible for following operations • Real-time localization analysis and rendering (sCMOS optimized) • Real-time FRET trace analysis • Automated 3D sample scanning and acquisition • Clustering and co-localization analysis • Residual drift correction • Scripting interface; OMERO compatibility

17 Installation and training Installation and training at the user’s facility, for a minimum three days by a trained system engineer. This will be followed by additional training sessions after one month and six months of installation. Thereafter, at least one training session will be conducted every six months for next five years.

18 Warranty On-site comprehensive warranty for a period of 5 years for the full system, reagents, consumables (LNP profiler Kit, EV profiler Kit, DNA paint kit, dSTROM discovery kit, dSTROM training kit), and spare parts, Warranty service should include breakdown repairs of full system, demonstration, shifting, Re-installation, during warranty period for any spares replacement the supplier will be responsible for all kind of logistics and custom clearance.

19 Payment term 90% against delivery (Rest 10%: 5% after satisfactory installation and 1% in every years for next five years for comprehensive warranty and satisfactory service).

20 Delivery periods 2 weeks from the date of receipt of purchase order.

Name of the firm-

Name of authroized signatory-

Indian Institute of Science Education and Research Bhopal

TECHNO-COMMERCIAL BID

Tender No.: IISERB/SP/2025-26/111 Dated 27.02.2026

Item: Supply & installation of Single-molecule Localization Super-resolution Microscope

1 Name of Tendering Company with Registration No. & Date issued by appropriate authorities. (Please enclose copy of certificate of registration)

2 Do you possess trade license issued by Competent Authorities in India? If so, please enclose a copy.

3 Name of Proprietor / Director

4 Furnish following particulars of the Registered Office a. Complete Postal Address

b. Telephone No. / Mob. No.

d. E-Mail Address

5 Furnish following particulars of the Local Branch Office. (if any) a. Complete Postal Address

b. Telephone No. / Mob. No.

d. E-Mail Address

6 PAN No. (Attach Attested Copy)

7 GSTIN No. (Attach Attested Copy)

8 If Manufacturer – Pl. attach the certificate of Registration

9 If Authorised Dealer / Distributer – Pl. attach relevant certificate.

10 Financial turnover for the three financial Years. (Please attach copy of certificate by Chartered Accountant in original )

Financial Year Amount (In Lakhs ) Remarks, if any

(Attach separate sheet if space provided is insufficient)

11 Give details of the major clients – Educational Institutes/Universities, Government Departments, Research Organisations, to whom item/material of same type have been supplied by the bidder during the last five years in the following format.

Sl. No Name & address of the client with details - Name of the contact person, telephone no., Fax no., e-mail id Name & quantity of the items sold Purchase Order Work Order No. & date Purchase order / Work Order amount (Rs. In Lakh)

(If the space provided is insufficient, a separate sheet may be attached)

12 The agency should not have been black listed or banned by any Govt. Department, Government Organization, PSU, University, Autonomous Institute etc. A notarized certificate to this fact should be enclosed with techno-commercial bid as per Annexure-V.

13 Are you an ISO certified manufacturer? If so, please attach a copy of the certificate.

14 Please specify the minimum time required to supply the item / material from the date of receipt of the Purchase Order.

15 Additional information, if any (Attach separate sheet, if required)

16 Agency who is not registered in the Institute Campus Automation System (CAS), get registered via. link: https://payeeregistration.iiserb.ac.in/ If you have already registered, please mention reference code & date. This is for the release of EMD, payment etc., so it is mandatory, without this no payment is possible.

Name of Authorised Signatory:

Name of the Company:

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Tender Inviting Authority: Indian Institute of Science Education and Research Bhopal (IISERB)

Name of Work: Supply & installation of Single-molecule Localization Super-resolution Microscope.

Tender No: IISERB/SP/2025-26/109 Dated 27.02.26

Name of the Bidder/ Bidding Firm / Company :

Sl. No. Item Description Item Code / Make Quantity Units Estimated Rate in Rs. P rate Addition / Deduction Addition / Deduction Values Currency Convertion against each Item Quoted Currency in INR / Other Currency BASIC RATE In Figures To be entered by the Bidder in Discounted Offered Any other charges Basic Amount IIIrd Party i.e DGS&D / RITES etc Inspection Charges @0.34%+Service Tax in Rs. P Less for Cenvat Credit,if any respect of Supplies Under full Excise Duty Category in Rs. P GST ( percentage) GST (Amount) RS P Ex-works price FCA price FOB price CIP price (Delhi / Mumbai) FOR IISER BHOPAL Price FCA /FOB Port name HSN Code CIP AirPort

1 Supply & installation of Single-molecule Localization Super-resolution Microscope. item1 Single 0.0000 Excess(+) Full Conversion USD Xahsgaf

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