Supply of Laboratory Item for Research Purpose under Sponsored Project

List of Items

His 60 Ni Superflow Resin (Clontech#635660)-25 ml

Lysozyme (Mol Bio grade)-25g

DTT (Mol Bio grade)-5g

ProteaseArrest™ [100x]-2ml

Wizard Plus SV Minipreps DNA Purification kit-500 prep

Taq DNA Polymerase [2x] Mastermix -1000 µl

DNAmark™ 1kb PLUS Marker- 50 µg

PAGEmark™ Tricolor PLUS -100 loads

LB Broth-250 g

Nickel Sulphate AR (hexahydrate) (Mol Bio grade)-500 g

Ampicillin (Sodium) (Mol Bio grade)-25 g

IPTG (Mol Bio grade)-100 g

Iodoacetamide (Mol Bio grade) -5g

EDTA, Na2 (Mol Bio grade)-500g

Agar-500g

Agarose I (Low EEO) (Mol Bio grade)-500g

Ethidium Bromide (Mol Bio grade)-100g

Coomassie blue R-250 (Mol Bio grade)-10g

Calcium Chloride (Dihydtrate) -500g

Magnesium Chloride -100g

Sodium Chloride -500g

Potassium Chloride -500g

POTASSIUM HYDROXIDE Pellets AR-500g

Sodium Hydroxide -500g

n-BUTYL ALCOHOL AR (n-butanol) (Mol Bio grade)-500 ml

ACETIC ACID GLACIAL AR -500 ml

Glycerol (Mol Bio grade)-500 ml

DMSO (Mol Bio grade)-100 ml

Methanol AR-500 ml

Isopropyl Alcohol AR (Mol Bio grade)-500 ml

Albumin Bovine V (BSA) -100g

Triton® X-100 (Mol Bio grade)-100 ml

SDS (Mol Bio grade)-100g

Acrylamide (Mol Bio grade)-500g

Bis-Acrylamide (Mol Bio grade)-100g

Tris Base (Mol Bio grade)-500g

HEPES (Mol Bio grade)-100g

MOPS (Mol Bio grade)-100g

PMSF(Mol Bio grade)-5g

TEMED (Mol Bio grade)-50 ml

Ammonium Persulphate (Mol Bio grade)-100g

β-Mercaptoethanol (Mol Bio grade)-100 ml

pH Meter, with 5 point calibration • pH Range : 0.00 – 14.00 • pH Accuracy : +/-0.02 • pH Resolution : 0.01 • mV Range : +/-1999mV • mV Accuracy : +/-0.2mV (-199.9 – 199.9mV) or +/- 2mV others • mV Resolution : +/-0.1mV (-199.9 – 199.9mV) or +/- 1mV others • Temperature Range : 0 ~ 80.0°C • Temperature Accuracy : +/-0.5°C • Temperature Resolution : ±1°C • LCD size (mm) : 40 x 105 • Operating Temp. & RH : 0°C – 50°C, Humidity <80% • Storage Temp. & RH : -20°C – 60°C, Humidity <90% • Dimension (mm) : 317 x 168 x 58 • Weight : 150g • Power : Adaptor, 9VDC <10Ma • Accessories Included : Meter/ USA Plug type adaptor/ Manual/ Probe/ Solution/ Cable/ Software CD/ Probe stand

Water Bath (Non-circulating), Amb.+5° ~ 100° C (10 Ltrs.) Temp. Range : Amb.+5°~100°C • Temp. Accuracy : ± 0.1°C • Sensor : PT-100Ω • Digital timer : 999h 999min 999sec • Inner Dimension : 295 x 235 x 150 (mm) • Outer Dimension : 410 x 280 x 170 (mm) • Weight : 6 kg • Power : 220v 60Hz • Accurate temperature control • With drainage stopcock for easy cleaning • PID controlled speed and temperature with LED digital display • Made with SUS 304 stainless steel body with powder coating

Gel Rocker, 0 – 50 Oscill / min, Movement: See- Saw Motor: PMDC Motor Platform size: 250 x 250 mm Timer: 0 – 60 min Overall dimension: W x D x H (260 x 283 x 180)mm Rubber mat:Non Slip rubber Mat Operating volt: 230v 50Hz, Single phase

Hot Plate Stirrer, Max. Speed : 200 ~ 1500 rpm • Temperature range : Amb.+5 ~ 380°C • Capacity : 2 Ltrs. • Display : Temperature, RPM and Time • Platform Size : 150 x 150 (mm) • Adjustable safety cut off : 50 ~ 400°C • Dimensions : 310 × 180 × 110 (mm) • Weight : 3.5 kg • Power : 230/115 V 50/60 Hz • Ceramic coated plate, chemically resistant and scratch proof. • Even heat distribution • Thermal insulation for cool body operation • Digital set up temperature controller with independent LED display. • Maximum heating plate temperature upto 380°C • Single multi-turn intelligent knob operation.

Tip Box, 10ul - 96 well

Tip Box, 200ul - 96 well

Tip Box, 1000ul -100 well

Micropipette 0.5-10ul (Mol Bio grade)

Micropipette 2-20ul

Micropipette 20-200ul

Micropipette 100-1000ul

DNA Electrophoresis, The horizontal electrophoresis unit must include 10X7 cm and 10X10 cm trays. • Gel Trays should be UV transparent. • Lid and cables must be separate and should not be fixed. • Should be able to run 100 samples. • Run time should be 45-60 min at 90. • Should include 1 mm thick 2 combs of 16 sample wells. • Buffer volume should not be more than 300ml. • Should have multichannel compatible combs for speed loading. • 16 sample combs should have 16 µl sample capacity in each well. • Should have cassette type electrodes. • Should have pure platinum wire and gold plated corrosion resistant electrodes. • Should include casting dams for tape free casting of gels. • Should have color coded combs option and should have options for 0.75mm, 1mm, 1.5mm and 2mm combs. • The unit must have Safety Requirements for Electrical Equipment for measurement, Control, and Laboratory Use – Part 1: EN61010-1: 2001

Miniprotein SDS Page unit with transfer tank and power supply • Power supply should have 5 pair of output to connect 5 units all together in the same unit. • It should be programmable to set different programs. • Display should be 2.4” LCD display. • It must include preset programs and must have 6 steps with 30 programmable files. • Should have timer to 999 minutes and timer alarm too after the end of run. • When target constant mode is set, the system automatically adjusts the two other parameters to maximum to allow for a constant run. • Safety Feature: No Load detection, Leakage detect, Overload detection, over temperature protection, & sudden load change detection. • Operating temperature 4℃~ 40℃. • The unit must have Safety Requirements for Electrical Equipment for measurement, Control, and Laboratory Use – Part 1: EN61010-1: 2001 • The system should be durable and leak-proof for complete safety and long life. • The system should be Compatible with all major brands of precast gels. • The system should allow Rapid Gel Casting and Loading ensuring trouble free gel set-up. • The system should have interchangeable modules for PAGE and Blotting using a single universal buffer tank. • The system should transfer up to 4 Mini gels. • The system should have complete run-time flexibility from overnight to as short as a couple of hours. • The system should have cool pack for environment friendly low-cost cooling during high-intensity transfers. • The system should have color-coded Cassettes to prevent polarity reversal to ensure transfer in the correct orientation. • The system should have easy to use Sliding Gates/Clamps for perfect sealing of glass plates. • The system should have color coded combs. • The system should have Corrosion resistant, 99.99% pure platinum electrodes and gold plated terminals which can be easily replaced, ensuring minimum repair times. • The system should have reversible combs to serve as loading indicators to aid pipette-well alignment, preventing sample loading errors. • Cassettes should have a rigid construction to ensure an even contact between the gel and the membrane and to maintain an even pressure.

Dodecyl β-D-maltopyranoside Dodecyl β-D-maltopyranoside-25g

Bradford reagent for protein estimation (Mol Bio grade)-500 ml

Glycine powder-500g

Relaprep RNA Cell Miniprep system-250 prep

Dual Glow Luciferase Assay System-100 ml

Wizard SV Gel and PCR Cleanup system-250prep

PureYieldTM Plasmid Midiprep (250 rxn)

MIinElute Gel Extraction kit-250 prep

anti-βarr antibodies -200 µl

p44/42 MAPK (Erk1/2) Antibody-200 µl

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody -200 µl

Anti-rabbit IgG, HRP-linked Antibody (Sigma-Aldrich-2 ml

Vortex

Steri pipette

Hot Air Oven (18X18X18)

Autoclave 22L (Automatic) • Chamber Size : 250 x 450mm (10x18) • Capacity : 22 Ltrs. • Sterilizing pressure : 1.2 kgf / cm (15psi)at 121°c • Pressure Gauge : 1.2 kg/cm² to 2.0 kg/cm² (30 psi) • Operating pressure : From 15 to 20psi (Adjustable) • Lid Tightening : Radial Locking system worked by handle • Lid Opening : Paddle lifting device • Water Level Indicator : Fitted on the outer chamber • Boiler – Hydraulically Tested : Upto +40 psi • Water Draining System : Hand Operated Valve • Heating : BY ISI Marked Immersion Heaters • Inner chamber made of stainless steel 304 grade. • Outer Body constructed from S. S. 304 Grade / Mild steel powder coated • Lid Closing by Radial Locking System • Sealing of Lid by Neoprene/Silicon Rubber Gasket • It is provided with Neoprene/Silicon Gasket, pressure gauge, pressure release valve S. S. Basket, safety valve, water level indicator & Digital Temp. Controller cum Timer (In-built) • Operating range from 5psi to 20psi pressure • Sterilizing Temperature up to 134°C • Water level cut off device • Pressure control switch • Tested up to 2.5 times of required Pressure

Cryo boxes

Transilluminator Transilluminator , • Outer Dimension: 280 x 340 x 80 (mm) • Viewing surface : 210 x 260 (mm) • Wavelength : 302 & 365 nm • UV tubes : 9 x 8W • UV blocking cover : Aluminum alloy frame • Uniform lighting design, Ultra High Contrast! • Ultra Safe UV blocking: block 99.99% UV transmittance • Strong and stylish metal frame design (NO breakage of UV shield) • Fast turn on speed for UV lamps • Dual UV wavelengths: 302nm and 365nm • Adjustable intensity: from 100% down to 50%

4 degree Freezer, 450 l

Freezer (-20 degree) , 350 l • Inner wall - S.S wall polished 304 quality Steel • Outer wall - M.S with powder Coated • Insulation - 75 mm mineral wool • Temp. range - Ambient to -20°C • Temp Controller - Microprocessor Based Controller • Accuracy - 0.5°C • Uniformity - 1.0°C • Air circulation - By motor and blower • Heater - Placed three side in wall

Digital Orbital Shaking Incubator with Cooling Triple walled chamber, outer Chamber of Mild steel sheet duly Powder Coated., inner chamber made of Polished Stainless Steel sheet S.S. 304 grade. clamps for 250ml, 500ml, 1000ml, 2000ml which can easy be fitted by easy screw openings CFC free Refrigeration system Control Panel Consisting of Mains Switch, PID, Temperature Controller, Digital Speed control Drive chamber size; 24"x24"x30" (WXDXH).

Immune- Blot PVDF Membrane, 26CMX x 3.3 m , 1 roll per pack

D R scientific Instruments