Rate Contract of Chemical Reagents Disposable and Kits for HLA Lab

Name of Kits

HLA typing kits A,B.DR by SSP molecular techniques-demo required

Positive Controls for cross matching( bag / Inotrain)

Negative Controls for cross matching (bag / Inotrain)

Celiac disease HLA Typing kits DQ2& DQ8 by SSP molecular techniques -demo required

HLA B 27 PCR SSP Technique-demo required

DNA Mini Kit extraction from blood.

Name of chemicals

Proteinase K ( molecular grade)

Rabbit Complement ( molecular grade)

5% Aquas Eosin( molecular grade)

RPMI Media -1640 with out L-Glutamine ( molecular grade)

Propan -2-OL (iso – propyl alcohol) ( molecular grade)

10 x TBE Buffer ( molecular grade)

Taq polymerase ( molecular grade)

Ethidium Bromide ( molecular grade)

Electrophoresis Matrix low EEO Agarose / powder ( molecular grade) Gelling Temp. (1.5%) 34.50C – 37.50C , Moisture : ≤ 0.10%, Sulfate : ≤ 0.15% EEO (mr) : 0.09-013, Gel strength (1%) :≥1, 200g /cm2, DNase /RNase: Non Detected , Store Dry (180C - 260C)

Lymphoprep density gradient separation media→ Ready made ,sterile & endotoxin tested solution. Sod. Diatrizoate – 9.1 % ,Polysaccharide – 5.7% Density- 1.077±0.001g/ml, Osmolality – 290 ±15m Osm Endotoxins- <1.0EU/ml

Napathol As phosphate ( molecular grade)

Fast Blue BB salt ( molecular grade)

Neutral Red AR C15H17CLN4 ( molecular grade)

3,3-diaminobenzidine (DAB powder) C12H14N4 ( molecular grade)

Formalin (AR)

Chloroform (AR)

Distilled water

Methanol-AR

Lishmen stain power (AR)

Nuclear free water (molecular grade) Need smaller packing

Immersion oil (Microscopy grade) 150 ml

Savlon Liquid soap

Hand sanitizer antiseptic solution.

Metabisulphate (Molecular grade)

Phenol red(Molecular grade)

Barium Sulphate

DNA Ladder 100bps(Molecular grade)

Ammonium Choloride (AR)

Pot. Hydrogen Carbonate (AR)

EDTA Powder (AR)

Sodium hydroxide Pallets (AR)

Sodium chloride NaCl (AR)

Sodium dodeyle sulfate (AR)

Hydrogen Peroixde (AR)

Potasium dihydro phosphate (AR)

Disodium hydrogen phosphate (AR)

Disodium hydrogen phosphate dihydrous (AR)

Ethanol molecular grade (AR)

Isoamyl Alcohol (Molecular Grade)

Sodium Hydroxide Pellets

Surface Cleaning Solution

Name of Disposable Items

Disposable Dropper up to 2 ml mark

15 ml conical graduated tube with screw cap & label -Sample Required

Rabbit complement cups

Disposable Tips yellow upto 1-10 ul (Setrile tips in boxes DNAs,RNAs free ) 96x10 -Sample Required

Disposable Tips White upto 10-100 ul (Setrile tips in boxes DNAs,RNAs free ) 96x10-Sample Required

Disposable Tips Blue upto 100-1000 ul (Setrile tips in boxes DNAs,RNAs free ) 96x10-Sample Required

Terasaki trays (60 wells)

Ependrof Tube Autoclavable with conical bottom 1.5 ml with snap lock frosted cap-Sample Required

Cryolabels for ependrof tubes

Filter paper watman No. 0 size 12.5 cm

Nitrile powder free gloves 6.5 No (Sterilized)

Nitrile powder free gloves 7 No (Sterilized)

Shoe cover (plastic disposable )

PCR Tubes. 0.2-0.5 ml with lid. -Sample Required

Tissue paper roll

Micro pore roll.

KIM WIPE PAPPER

PARAFILM (41N X 125 FT) ROLL

Temperature recording paper of -80 degree deep freezer

Almunium foil

50 ml conical graduated tube with screw cap & label

CHEMICALS INDIA

Global Biomedical