NIT No. 23 Item No.1

REAL TIME PCR SYSTEM for WATER TESTING LAB 1. An automated integrated system for both real-time PCR and post-PCR (end-point) analysis using in-built 96 well based on Peltier technology for heating and Cooling. 2. The instrument should have the open software that allow control of thermal cycling protocols to further assist in developing or running custom assays. 3. The instrument should be truly open platform i.e. user can easily use any company’s whole kits, consumables and reagents as well as various other chemistry like Sybr Green, TaqMan, and also HRM dyes etc. 4. The instrument should have flexibility to use individual tube, strips or 96 well plates which are available from any other brands. 5. The system should have block temperature range of 4°C - 105°C to facilitate incubation of samples at low temperature of amplified material for other downstream applications. 6. The system should have gradient feature (of temperature span of 1-36°C), which can help users for the optimize PCR conditions. 7. The Instrument should have advance optical system with the broad range of Emission and Excitation i.e. 300 to 800nm. 8. The instrument must have emission filters that are optimized to be able to detect a broad range of fluorophores including FAM, SYBR Green, HEX, TET, JOE, VIC, ROX, Texas Red, Cy5, and Cy5.5 etc. with option to use any other custom dye falling within the available wavelength range without the requirement any calibration kit. 9. The system should have peak block ramp rate for (heating as well as cooling) 4°C/S. 10. The system should have minimum 5 or more filters which enable to do the multiplexing for minimum 5 colors or more in single tube. 11. The instrument must provide specialized software which capable to collects and analyzes the fluorescent data for the applications of Absolute quantification, Relative quantitation, Allelic discrimination/Single nucleotide polymorphism detection with auto call and manual call option, Melt curve genotyping analysis and HRM etc. 12. The system software must have the Crosstalk Correction and Background Correction inbuilt capability without any extra cost. 13. The System software must provide user to import standard curve to analyze and save experiment resources. 14. System should be capable to perform High Resolution Melting curve analysis (0.1 C for better data acquisition) for genotyping , mutation scanning studies and etc 15. The instrument should have highly advance optical system like LED or tungsten-halogen lamp for excitation and PMT and does not required any reagents or kit for calibration of system. The advance optical system will also eliminated the use of reference dyes. 16. The Dynamic range should be 1-10 logs with temperature accuracy: ±0.1°C; Temp. Uniformity: ±0.3°C (at 55°C). 17. System should have advance aautomatic hot-lid technology with adjustable temperature range from 30 to 110OC and needs no manual opening/closing and ensures constant pressure and reduce evaporation so that user can min reaction volume of 5-100µL 18. The system must accomplish with branded Laptop with software installed and also online UPS for the smooth running of system. 19. Supplier must supply the following kits & consumables along with the Real Time PCR Machine as free of cost: A. Water DNA/RNA Extraction Kits (200Rxn) each. B. Real Time PCR Water Diagnostics kits (200 Rxn each) to detect 1. Vibrio cholera 2. Salmonella spp. 3. Enteroviruses 4. Hepatitis A 5. COVID-19 Make : Himedia, Thermofischer scientific, eppendorf and Qiagen

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