Procurement of SYN GENE 0-3 (Gene Synthesis)

Procurement of SYN GENE 0-3 (Gene Synthesis)

Gene 1. GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 2 MRGSVLPVATSVSRTTHHAPAALGVYGGYGLGAYGFGYPGATVSHTTHHAPYGFGGLYGGYGGLLHGGLYGGYGLGAYGFGYPAATVSHTTHHAPYGYGGLYGGYGGLLHGGLYGGYGLGAYGFGYPAATVSQTTHHAPYGYGGVYGGYGLLHGGLYGGYGLGGYGLGYPAATAVSHTTHHAPLGYGGLYGGYGGLLHGGLYGGYGATAVSHTTHHAPLGYGLAGYGGLYGGFLH

Gene 3. GPCCQLSYGYDEKSTGGISVPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYSAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPPQLSYGYDEKSTGGISVPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYSAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPPQLSYGYDEKSTGGISVPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA

Gene 4. GPCCQLSYGYDEKSTGGISVPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYSAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPPQLSYGYDEKSTGGISVPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYSAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPPQLSYGYDEKSTGGISVPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA

Gene 5. GPCCGPPGPPGPPGPPGPPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGRPGKRGKQGQKGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGFPGERGVEGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 6. GPCCGPPGPPGPPGPPGPPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGAKGSAGAPGIAGAPGFPGPRGPGPQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGPRGQPGVMGFPGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGRKGRGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 7. GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGYQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 8. GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGYQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPKGEQGPTGPTGPAGPKGLQGLQGLQGEKGEQGPTGPAGPKGLQGEKGEQGPTGLAGKAGEAGAKGETGPAGPQGPKGEQGPQGLPGKDGEAGAQGPAGPMGPAGEKGEKGEPGTQGAKGDKGETGPVGPKGEKGEAGPAGKDGEKGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 9. GPCCGPPGPPGPPGPPGPPGSPGLPGPKGEQGPTGPTGPAGPKGLQGLQGLQGEKGEQGPTGPAGPKGLQGEKGEQGPTGLAGKAGEAGAKGETGPAGPQGPKGEQGPQGLPGKDGEAGAQGPAGPMGPAGEKGEKGEPGTQGAKGDKGETGPVGPKGEKGEAGPAGKDGEKGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPKGEQGPTGPTGPAGPKGLQGLQGLQGEKGEQGPTGPAGPKGLQGEKGEQGPTGLAGKAGEAGAKGETGPAGPQGPKGEQGPQGLPGKDGEAGAQGPAGPMGPAGEKGEKGEPGTQGAKGDKGETGPVGPKGEKGEAGPAGKDGEKGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 10. ADEQEEKAKVRTELIQELAQGLGGIEKKNFPTLGDEDLDHTYMTKLLTYLQEREQAENSWRKRLLKGIQDHALDLVPRGSPGYPGPRGEQGPTGPTGPAGPRGYQGYQGYQGERGEQGPTGPAGPRGYQGERGEQGPTGYAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKY

Gene 11. GPCCGPPGPPGPPGPPGPPGSPGYPGPRGEQGPTGPTGPAGPRGYQGYQGYQGERGEQGPTGPAGPRGYQGERGEQGPTGYAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGYPGPRGEQGPTGPTGPAGPRGYQGYQGYQGERGEQGPTGPAGPRGYQGERGEQGPTGYAGKAGEAGAKGETGPAGPQGPRGEQGPQGYPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGYPGKDGKDGQNGKDGYPGKDGKDGQNGKDGYPGKDGKDGQDGKDGYPGKDGKDGYPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 12 MKWFLFLLTTAVLAAVVSAHEEDGVCNSNAPCYHCDANGENCSCNCELFDCEAKKPDGSYAHPCRRCDANNICKCSCTAIPCNEDHPCHHCHEEDDGDTHCHCSCEHSHDHHDDDTHGECTKKAPCWRCEYNADLKHDVCGCECSKLPCNDEHPCYRKEGGVVSCDCKTITCNEDHPCYHSYEEDGVTKSDCDCEHSPGPSE

Gene 13 MLLRPVLLLAALAALAAATGSRPYFPVSSLKPVLSGIGLPAFYKPDYALSGLVGYLNTRPKIVTQAQFTARIQKYAPVIKRIVLPMRRKYSGILGDLIQVAVIRYYGCEPVIGSSIHLDRIFGEYIKRQSMPSPYKYNAKYVTGFIRGFMGYMHKNYKPSQLVVPVVKPTYPGYGLLTVLQSVGLPALTNPRMSLGGVVAYLQLANIQQAVFISRIRSQRKAIRRLVSKYRSRYSGVQLDLLCLAALRYYGVPRTARYVVDFDYALEHSLKSTAIVHYNPSYVRTFLSRFSTKLVTLPYPGYDMITIFRGFGLPKLYQPRYTLGGLVSYLKVAKISQPTFIGQIKKYAKKIKKFIRKYKKKYSGYRSDLLQLAAIRYCLYPRSYPIKFSTVFQRALSSYSTYSVSSVSSFLGLFTQYLKKPAYVGYNLKPVLLQAGLPKLSMPQYSLSGLMSYIHGNKYSDSSLIGLIRVYGPKIKRIVHQYKSRYSGIQADLLQLCAIRYYSLPVVFRSSYSFGTIFQQYLGSQNLKVYNASTVKRFINGFVSYIRKRQSNKYSGPMWVCRRC

Gene 14. MKFLVVTLALLAAAHAFPLGEAEVPAINDGYSSVSEEKPAVPEVKAAPEAVPATVAEPAVIAAPVEAAVPAISEVPAITAVPAEVAAPVEAVAEEVKPAVAVEKAAEPIVEEKAPVKQEQIPAVAVDLAPKPEDAPKIEAKEAAIELPAEKPVDVIQAAIVPAAVAEESIAPQIKSASEEIPKPEKKIDLIPEPEIKSLPIVPVVPEAASIIPSAPVAVEAPVEAKPVESAPEKREVSAELIEKVEPIAKPAEDKPAEVPAVKAEEPAKVEAVKEEAKVEAVEPAKPVVVEAVKPVIVDAVKEEKAAPAKEEVAIPAVEAVQEVKVPEVAEPKAVVAEVAPVADAVRVVRDTLLENQTETAKEELKPEIVKVAEEVKVEAPKAEEAKPEVAKIAEEAKPVEAKVEAPQVEEVKPVIAEPVKAEEKIAEPIKAEEKIAEPIAAPVKVAEPEKAAEVPAKVADASNESNESEEKKVSAESAPIKGDVLPVAVPQVAAEEIKSSESSESKSASVEDKSNESVEPIKEVSKEVKEEKSQEAVSDEKSS

Gene 15. Fusion protein – the gene should have BamH1 (ggatcc) and HindIII (aagctt) on both the ends of the gene sequence. Restriction site Kpn1 and Sal1 is present in between anx and GFP gene.(Additional kpnI site removed) Xba I-BamH1-Annexin- KpnI – Linker – SphI – GFP- PstI-XhoI ggatccatggcacaggttctcagaggcactgtgactgacttccctggatttgatgagcgggctgatgcagaaactcttcggaaggctatgaaaggcttgggcacagatgaggagagcatcctgactctgttgacatcccgaagtaatgctcagcgccaggaaatctctgcagcttttaagactctgtttggcagggatcttctggatgacctgaaatcagaactaactggaaaatttgaaaaattaattgtggctctgatgaaaccctctcggctttatgatgcttatgaactgaaacatgccttgaagggagctggaacaaatgaaaaagtactgacagaaattattgcttcaaggacacctgaagaactgagagccatcaaacaagtttatgaagaagaatatggctcaagcctggaagatgacgtggtgggggacacttcagggtactaccagcggatgttggtggttctccttcaggctaacagagaccctgatgctggaattgatgaagctcaagttgaacaagatgctcaggctttatttcaggctggagaacttaaatgggggacagatgaagaaaagtttatcaccatctttggaacacgaagtgtgtctcatttgagaaaggtgtttgacaagtacatgactatatcaggatttcaaattgaggaaaccattgaccgcgagacttctggcaatttagagcaactactccttgctgttgtgaaatctattcgaagtatacctgcctaccttgcagagaccctctattatgctatgaagggagctgggacagatgatcataccctcatcagagtcatggtttccaggagtgagattgatctgtttaacatcaggaaggagtttaggaagaattttgccacctctctttattccatgattaagggagatacatctggggactataagaaagctcttctgctgctctgtggagaagatgacggtaccggtggctctggatctggcggttctggatctggtggctctggctccggtggctctggatctggcggttctggatctggcatgcatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggcgatgtgaacggccataaatttagcgtgcgtggcgaaggcgaaggcgatgcgaccaacggcaaaattaccctgaaactgatttgcaccaccggtaaactgccggtgccgtggccgaccctggtgaccacctgcggttatggtgtgcagtgctttgcacgttatccggatcatatgaaacgtcatgatttctttaaaagcgcgatgccggaaggctatgtgcaggaacgtaccattagctttaaagatgatggcacctttaaaacccgtgcggaagtgaaatttgaaggcgataccattgtgaaccgtattaaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactttaacagccataaagtgtatattaccgcggataaacagaaaaacggcattaaagcgaactttaaaattcgtcataacgtggaagatggcagcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgcgtctgccggataaccattatctgagcacccagagcgtgattctggaagatccgaacgaaaaacgtgatcatatggtgctgcatgaatttgtgaccgcggcgggcattacccatggcatggatgaactgtataaactgcag

Gene 16. For pET vector expression, the sequence should be cloned in pET303_CT-His_A118. however, add stop codon in the ORF before histag to avoid expression of protein with Cterminal His tag. GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 17. For pET vector expression, the sequence should be cloned in pET303_CT-His_A118. however, add stop codon in the ORF before histag to avoid expression of protein with C_terminal His tag. GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG GPCCQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYSAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPPQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA

Gene 18. For pET vector expression, the sequence should be cloned in pET303_CT-His_A118. however, add stop codon in the ORF before histag to avoid expression of protein with C_terminal His tag. GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 19. Without Histag in pQE80L – Add the RBS and other sequence (Clone in EcoRI and PstI) Scl2 three domains with 41 GXY repeats similar to Col108 For pET vector expression, the sequence should be cloned in pET303_CT-His_A118. however, add stop codon in the ORF before histag to avoid expression of protein with C_terminal His tag. GAATTC ATTAAA GAG GAG AAA TTAACT ATG AGA GGATCG GPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 20. DDDDKGPCCGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 21. GPCCGPPGPPGPPGPPGPPDEQEEKAKVRTELIQELAQGLGGFEKKNFPTLGDEDLDHTYMTKLLTYLQEREQAENSWRKRLLKGIQDHALDLVPRGSPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGGPPGPPGPPGPPGPPAAGVMGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGGPPGPPGPPGPPGPPAAGVMGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 22. CSQPLDVILLLDGSSSFPASYFDEMKSFAKAFISKANIGPRLTQVSVLQYGSITTIDVPWNVVPEKAHLLSLVDVMQREGGPSQIGDALGFAVRYLTSEMHGARPGASKAVVILVTDVSVDSVDAAADAARSNRVTVFPIGIGDRYDAAQLRILAGPAGDSNVVKLQRIEDLPTMVTLGNSFLHKLCSGFVRICTG

Gene 23. MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEADYHGNKQYGGRYGNRFGGGIGGAGAVAHAHAHAHASAGANGRARAHARALAHAHAGGSAAHGHPGFPVGGSASAAARAAARASAGGLGGLGGFGSAAANAAAAANAGAGFGGFGGFGGFGGVGGPGQPGGPGGPGGPGGPGGPGMPGGPGGPGGPGTGGPGQPGGPGGPGGPGGPGGPGMPGGPGGPGGPGMPGGPGGPGGPGVGGGDGGIPGMPGPAGPPGPAGPQGPEGEQGPRGRTPAGTPGPPGNPGEPGQGGAPGAPGAPGHAGKPGTAGPAGKAGRPGPRGQSGASGSSGQHGASGAPGRPGNPGSTGRPGATGDPGRPGATGTTGRPGPSGAPGNPGAPGALGAPGPRGAPGFVGLPGPRGSPGEPGNQGPIGGPGYPGPRGPQGPDGAMGPQGPCGDRGAPGVPGKQGPVGGQGPAGPRGPRGDEGPVGPKGEPGAKGADGKPGDRGPDGETGPQGPAGPKGQVGDQGKPGAKGETGDQGARGEAGKAGEQGPGGIQGPKGPVGGQGPAGPAGPLGPQGPMGERGPQGPTGSEGPVGAPGPKGSVGDQGAQGDQGATGADGKKGEPGERGQQGAAGPVGRPGPRGDRGAKGIQGSRGRPGGMGRRGNRGSQGAVGPRGETGPDGNQGQRGEQGAPGVITLVIEDLRTAGVESPVETFDAGAGTGGPAPGAGATAGAFAGAGPGGANAGGNAAAGAGPGGLGGAGPGGLGGLGGGLGGLGGGLGGLGGAGGLGGGLGGLGGAGGLGGGLGGLGGAGGGAGGAGGAGGAGAIAAAAAQAAAAAGAGGNGGAAQAAAQAAASAAANSGLGAGAARAAASAAARATVAGHGSGTAAAAANAAAQAHAATRGQGGSHAHAAAAAHAAASSVIHGGGHGGHGGDYHGGDAGYHKPGYLEQKLISEEDLNSAVDGPPGPPGPPGPPGPPMRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEADYHGNKQYGGRYGNRFGGGIGGAGAVAHAHAHAHASAGANGRARAHARALAHAHAGGSAAHGHPGFPVGGSASAAARAAARASAGGLGGLGGFGSAAANAAAAANAGAGFGGFGGFGGFGGVGGPGQPGGPGGPGGPGGPGGPGMPGGPGGPGGPGTGGPGQPGGPGGPGGPGGPGGPGMPGGPGGPGGPGMPGGPGGPGGPGVGGGDGGIPGMPGPAGPPGPAGPQGPEGEQGPRGRTPAGTPGPPGNPGEPGQGGAPGAPGAPGHAGKPGTAGPAGKAGRPGPRGQSGASGSSGQHGASGAPGRPGNPGSTGRPGATGDPGRPGATGTTGRPGPSGAPGNPGAPGALGAPGPRGAPGFVGLPGPRGSPGEPGNQGPIGGPGYPGPRGPQGPDGAMGPQGPCGDRGAPGVPGKQGPVGGQGPAGPRGPRGDEGPVGPKGEPGAKGADGKPGDRGPDGETGPQGPAGPKGQVGDQGKPGAKGETGDQGARGEAGKAGEQGPGGIQGPKGPVGGQGPAGPAGPLGPQGPMGERGPQGPTGSEGPVGAPGPKGSVGDQGAQGDQGATGADGKKGEPGERGQQGAAGPVGRPGPRGDRGAKGIQGSRGRPGGMGRRGNRGSQGAVGPRGETGPDGNQGQRGEQGAPGVITLVIEDLRTAGVESPVETFDAGAGTGGPAPGAGATAGAFAGAGPGGANAGGNAAAGAGPGGLGGAGPGGLGGLGGGLGGLGGGLGGLGGAGGLGGGLGGLGGAGGLGGGLGGLGGAGGGAGGAGGAGGAGAIAAAAAQAAAAAGAGGNGGAAQAAAQAAASAAANSGLGAGAARAAASAAARATVAGHGSGTAAAAANAAAQAHAATRGQGGSHAHAAAAAHAAASSVIHGGGHGGHGGDYHGGDAGYHKPGYLEQKLISEEDLNSAVD

Gene 24 MLPAAILLLSLGAALSAPAPGVTPPVSPPLPPVPPPLPPKRAATDADAVTVGTLKTAGTAIGKSSGGAVSLEQTADQGSKAGVKVDLYSQRAGATEGSAASTSATKVKCKPGFSKGASVTGQQTSGASVGEATSTSDATGEAGTICDDTKSDVQGGASTSTISDGKAASEALNTAASEALNTAASETLTVPGGSSTVSNANSKTGGTSSGSAGTDAAGKASSRGIGDGTTSRADSQTKVSTTGDGRSEADQRSTGTGTTGRKRGALGAETSAQTTGSSATVGGGSDSKGESSAGGTANQGSNVAAESDSNQKIRSTRTGSSAVDAKSGSAAALGAIKDKLVGKSDAASGGSAESVGSAKTDFNTGGSAGHSAGEGSGFAETSVGGQTRQTGAVEGSQSSSASGSVTLKRPVWPCRLPSKAPKDWLHGWVPGTKLVWDCVIPHKVPAKYSKLYKPKW

Gene 25. MCKNGGTCTPNGMTFKCVCPSGWRGFTCKTADTTTAVVAESKPTSLAVQNCQKYSMCKNGGTCTPNGRTFKCLCPSGWRGFTCKTADTTTAVVAESKPTSLAVQNCQKYSMCKNGGSCTPNGKTFKCVCPSGWRGFTCKTPDTTTAVVVESKPTSLAVQNCQKYSMCKNGGTCTPNGRTFKCVCPSGWRGFTCKTADTTTAVVVESKPTSLAVQNCQKYSMCKNGGTCTPNGRTFKCLCPSGWRGFTCKTPDTTTAVVAESKPTSLAVQNCQKYSMCKNGGTCTPNGRTFKCVCPSGWRGFTCKTADTTTAVVVESKPTSLAVQNCQRYSMCKNGGTCTPNGRTFKCVCPSGWRGFTCKTPDTTTAVVVESKPTSLAVQNCQKYSMCKNGGTCTPNGKTFKCLCSSGWRGFTCKTPDTTTAVVVESKPTSLAVQNCQKYSMCKNGGTCTPNGRTFKCLCPSGWRGFTCKTADTTTAVVAESKPTSLAVQNCQRYSMCKNGATCTPNGKTFKCLCPSGWRGFTCKTPDATTAVVVESKSTSLAVQNCRRYSMCKNGGTCTPNEKTFKCLCPSGWRGFTCQAAAVTTTTASPVKNPSLKACQVNHLCRNGGTCQDAKNGFTCACAKDWQGARCTARRNLCSAERGPCQNGGTCSYSSQRGFLCGCTPAWQGETCQLVRDPCKTGLSCGSGFPCSRDPASLGGFSCDCDSRPDETGVEQSKVNG

Gene 26. GPCCGPPGPPGPPGPPGPP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GTG GGP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GMP GGP GGP GGP GVG GGD GGI GGG GMP GPA GPP GPA GPQ GPE GEQ GPR GRT GPA GTP GPP GNP GEP GQG GAP GAP GAP GHA GKP GTA GPA GKA GRP GPR GQS GAS GSS GQH GAS GAP GRP GNP GST GRP GAT GDP GRP GAT GTT GRP GPS GAP GNP GAP GAL GAP GPR GAP GFV GLP GPR GSP GEP GNQ GPI GGP GYP GPR GPQ GPD GAM GPQ GPC GDR GAP GVP GKQ GPV GGQ GPA GPR GPR GDE GPV GPK GEP GAK GAD GKP GDR GPD GET GPQ GPA GPK GQV GDQ GKP GAK GET GDQ GAR GEA GKA GEQ GPG GIQ GPK GPV GGQ GPA GPA GPL GPQ GPM GER GPQ GPT GSE GPV GAP GPK GSV GDQ GAQ GDQ GAT GAD GKK GEP GER GQQ GAA GPV GRP GPR GDR GAK GIQ GSR GRP GGM GRR GNR GSQ GAV GPR GET GPD GNQ GQR GEQ GAP GVI GPPGPPGPPGPPGPP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GTG GGP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GMP GGP GGP GGP GVG GGD GGI GGG GMP GPA GPP GPA GPQ GPE GEQ GPR GRT GPA GTP GPP GNP GEP GQG GAP GAP GAP GHA GKP GTA GPA GKA GRP GPR GQS GAS GSS GQH GAS GAP GRP GNP GST GRP GAT GDP GRP GAT GTT GRP GPS GAP GNP GAP GAL GAP GPR GAP GFV GLP GPR GSP GEP GNQ GPI GGP GYP GPR GPQ GPD GAM GPQ GPC GDR GAP GVP GKQ GPV GGQ GPA GPR GPR GDE GPV GPK GEP GAK GAD GKP GDR GPD GET GPQ GPA GPK GQV GDQ GKP GAK GET GDQ GAR GEA GKA GEQ GPG GIQ GPK GPV GGQ GPA GPA GPL GPQ GPM GER GPQ GPT GSE GPV GAP GPK GSV GDQ GAQ GDQ GAT GAD GKK GEP GER GQQ GAA GPV GRP GPR GDR GAK GIQ GSR GRP GGM GRR GNR GSQ GAV GPR GET GPD GNQ GQR GEQ GAP GVI GPP GPP GPP GPP GPP GPCCGYIPEAPRDGQAYVRKDGEWVLLSTFL

Gene 27. GPCCQLSYGYDEKSTGGISVP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GTG GGP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GMP GGP GGP GGP GVG GGD GGI GGG GMP GPA GPP GPA GPQ GPE GEQ GPR GRT GPA GTP GPP GNP GEP GQG GAP GAP GAP GHA GKP GTA GPA GKA GRP GPR GQS GAS GSS GQH GAS GAP GRP GNP GST GRP GAT GDP GRP GAT GTT GRP GPS GAP GNP GAP GAL GAP GPR GAP GFV GLP GPR GSP GEP GNQ GPI GGP GYP GPR GPQ GPD GAM GPQ GPC GDR GAP GVP GKQ GPV GGQ GPA GPR GPR GDE GPV GPK GEP GAK GAD GKP GDR GPD GET GPQ GPA GPK GQV GDQ GKP GAK GET GDQ GAR GEA GKA GEQ GPG GIQ GPK GPV GGQ GPA GPA GPL GPQ GPM GER GPQ GPT GSE GPV GAP GPK GSV GDQ GAQ GDQ GAT GAD GKK GEP GER GQQ GAA GPV GRP GPR GDR GAK GIQ GSR GRP GGM GRR GNR GSQ GAV GPR GET GPD GNQ GQR GEQ GAP GVI SAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPP QLSYGYDEKSTGGISVPGQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GTG GGP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GMP GGP GGP GGP GVG GGD GGI GGG GMP GPA GPP GPA GPQ GPE GEQ GPR GRT GPA GTP GPP GNP GEP GQG GAP GAP GAP GHA GKP GTA GPA GKA GRP GPR GQS GAS GSS GQH GAS GAP GRP GNP GST GRP GAT GDP GRP GAT GTT GRP GPS GAP GNP GAP GAL GAP GPR GAP GFV GLP GPR GSP GEP GNQ GPI GGP GYP GPR GPQ GPD GAM GPQ GPC GDR GAP GVP GKQ GPV GGQ GPA GPR GPR GDE GPV GPK GEP GAK GAD GKP GDR GPD GET GPQ GPA GPK GQV GDQ GKP GAK GET GDQ GAR GEA GKA GEQ GPG GIQ GPK GPV GGQ GPA GPA GPL GPQ GPM GER GPQ GPT GSE GPV GAP GPK GSV GDQ GAQ GDQ GAT GAD GKK GEP GER GQQ GAA GPV GRP GPR GDR GAK GIQ GSR GRP GGM GRR GNR GSQ GAV GPR GET GPD GNQ GQR GEQ GAP GVI GPP GPP GPP GPP GPP GPC C SAGFDFSFLPQPPQEKAHDGGRYYRA

Gene 28 GPCCQLSYGYDEKSTGGISVP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GTG GGP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GMP GGP GGP GGP GVG GGD GGI GGG GMP GPA GPP GPA GPQ GPE GEQ GPR GRT GPA GTP GPP GNP GEP GQG GAP GAP GAP GHA GKP GTA GPA GKA GRP GPR GQS GAS GSS GQH GAS GAP GRP GNP GST GRP GAT GDP GRP GAT GTT GRP GPS GAP GNP GAP GAL GAP GPR GAP GFV GLP GPR GSP GEP GNQ GPI GGP GYP GPR GPQ GPD GAM GPQ GPC GDR GAP GVP GKQ GPV GGQ GPA GPR GPR GDE GPV GPK GEP GAK GAD GKP GDR GPD GET GPQ GPA GPK GQV GDQ GKP GAK GET GDQ GAR GEA GKA GEQ GPG GIQ GPK GPV GGQ GPA GPA GPL GPQ GPM GER GPQ GPT GSE GPV GAP GPK GSV GDQ GAQ GDQ GAT GAD GKK GEP GER GQQ GAA GPV GRP GPR GDR GAK GIQ GSR GRP GGM GRR GNR GSQ GAV GPR GET GPD GNQ GQR GEQ GAP GVI SAGFDFSFLPQPPQEKAHDGGRYYRAGPPGPPGPPGPPGPP QLSYGYDEKSTGGISVPGQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GTG GGP GQP GGP GGP GGP GGP GGP GMP GGP GGP GGP GMP GGP GGP GGP GVG GGD GGI GGG GMP GPA GPP GPA GPQ GPE GEQ GPR GRT GPA GTP GPP GNP GEP GQG GAP GAP GAP GHA GKP GTA GPA GKA GRP GPR GQS GAS GSS GQH GAS GAP GRP GNP GST GRP GAT GDP GRP GAT GTT GRP GPS GAP GNP GAP GAL GAP GPR GAP GFV GLP GPR GSP GEP GNQ GPI GGP GYP GPR GPQ GPD GAM GPQ GPC GDR GAP GVP GKQ GPV GGQ GPA GPR GPR GDE GPV GPK GEP GAK GAD GKP GDR GPD GET GPQ GPA GPK GQV GDQ GKP GAK GET GDQ GAR GEA GKA GEQ GPG GIQ GPK GPV GGQ GPA GPA GPL GPQ GPM GER GPQ GPT GSE GPV GAP GPK GSV GDQ GAQ GDQ GAT GAD GKK GEP GER GQQ GAA GPV GRP GPR GDR GAK GIQ GSR GRP GGM GRR GNR GSQ GAV GPR GET GPD GNQ GQR GEQ GAP GVI GPP GPP GPP GPP GPP GPC C GYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA

Gene 29. Charge based adhesive protein (BamH1 and KpnI as cloning site) pQE80L and same as pET. GAGP[(GVGVP)(GKGVP9]8GWPH6 For an example: (GVGVPGKGVPGKGVPGKGVPGKGVPGKGVPGKGVPGKGVPGKGVPGKGVP)

Gene 30. MKVLTLLLPLLALVYSDDQLVDEGTIEDPSKLPPGYVLQPDGSYLMTIIEPGQETQEVQNQEQVHNEVIV QTEDQVQNEDDYSDYTYSNNILVVGDGDNKGVFKVSRGDSNNENDFSVEPVQSEDNPTPLSSVNKDDPQV YQEGTQGQDDYKVTLGVGKGKDRGYLQMMPDSSKPSGYKFVTQYISSYTIIPGDVQPNAEEPTEPDEEEI EVPDSPDIKQKKGYSVITVGRPGCRRYVKLTPSKNNKPHDFDLKPIKHPDQPDSSPDEDDDDAPEIIPNG ERGTPTYQPIIASGPSANRRYISVAQDPKQPKRFRLVPAPPVSTDNEATEPNDDDVEYEDVPENPQYTTV KVPGNKIPVGIVSLGHPKHRVHFKIVRRSNKPGDVQVEPIRDPHNLNSSPDLNNEYAPDVCQQGDPSDEN NAPIIATGPRFRRRYHKLLILRKGLRLIPVKRKRIAGTRKYKYILKKRRWHYRKIHKKIRKIIIHTKVTE RRRPHGFFWWSVWWYIWW

Gene 31. MKILLSVLVLLIVVECGNSRKIRHRGGSRRGSGGGSGGSSGGSSGGSDGSYGGSDGGSGGSYGDSGGGS GDSTGSNGGPGDSYSESGGSSGDGGSGGSYGGSDGGPGGSYGGSGGSGGGGGGGGGGGGGGSGSDNNPPEGYYDPNKSGPLPPGFEAPPGYEGGEWHPGPDGTMVRTIVEEEPGTETTELVPPDYDPQITPVGQPGSPGHDIIGVGKPGERGYFKRTPGPSGNPDDYTLEPIKSPENPEPVDPNDPNAPRVIRTKKNKNPTLQVGNKDNPQYFGLKPDPKNPGHFTLVPRKMHPGKPGHRKPGHPKGGKGKPGRGKPGHGKPGDGGALSPEEYEKLKQNPVMKYPVFTLGKPEHRRSFRITQDPNNPNDFSVDPIGNENDPQSPADPNDPEAPEIIPQGEKGKPDYNVVIGVNPKKKRDYVQMKPDPKNPQQFNFDPIFMEPQQEEPQQEQPTPAGGEPEVFLDPGSESVTPEEYERLKQNPKIDRKKGSPILHLGYEPYRRPFRIKQNRRNPLDFDVQPIGSDNDPNSKPDKDDPYKPVVYPLGEKGQPDYSQIIGVGPKDKRNYVRMIPDPKKPGKFEFEPVLLKPGEPIPFVTETEPPPTPTPEPTVPPPPPTHPPTTKKPEEEEPCFEDLPEDPEFRRVRHPGRRRPYQIISLGPKDHRQHFKKTPKSNNPDDYDIEPYDPDTPDHKPKADDPYAPEVIPQGQPGTPSFEPILMTGPKDNRKPYKLVHHPENPSRSSFVPVRPLPKKPGQKHPKFVNAPRQIRPRKKDEPRQPDDEPEEGEPEFTDLPDRPRFKHVKQPKRPYTLISVGKHPHRRTFKKTPNPNGNPDDYDLEPYDEDTPDHKPNPHDPSVPKVHKNGKKGTPDYQPVIEIGPINKRKLYKIKPHPSNPNKVEFVPVKKIMRKGKPFFKELPRRTATKRFRPRTTPKPNEDDDVPDDLPYDPEVRPHRTPGQKYPTQIISVGKPPNRKHFKKIPKPEDPKKFDLEPIDPDDPDEPLDPEDPNTPKVYPNPEGNPVIETGTPDDPKHHEVNPDPEDPEDPERVSFTPVKRLGEPGEKNPKFEKIPRKSHPFHTKRPEEATAPEPEENEPVFEDIPYNPTFKTLHRKRPDRPAQIIALGRPPHRQEFIKEPGRSGKPHDFKLSPYDSRRPDKKPDPNNPNKPEVYPARGKPGKPGYQPPVIKTGPKDDPEYFEIHPDPSDPKNPNKVLFVPVKARPGKPGTRPKFSPKEPRRYHPYHDPLKPRPTTEEPEDGTEYFVDQPEDPEVTTLFRPLRKYPSQLIAVGKPPNRKYFEKKPGPSGHPKDYTLHPIDPKHPDKSLNPKDSKTPKCFPGEGKPGTPNYKPPVIQTGSPDNPKFYRVIPNPKDPNNPESVEFIPVKKDKKNPKKFKPLPRNVRIYHTTKKQATSQPNQTPPHEEGDPYFTDLPDNPVVLTVRKPGSKKPVQILALGKPPHRKYFEKVPEPGKPDRFTLKPINPNGKPGEPSKVYPGGNKPGSRYPVIEYGKPPKAFEVRPDPLHPNDPTKVQFVPVKKVPGTDGPSFVPAPRTVGKYHTTRKKHHKTTKEPKEGSVIYDDGPYLPKFYPGKKNTKIVAVGKPPKRRYFKIIPKPDKKFTLKPIDPQNPDDPNPHPEDTKVYPQPDGHPVFEIPGPNKKPEYFKLVPKKDNPKEMDFIPVRPVSPGSFTSKPRTTRHYKTTKKSKRTTRPTSRVIFEDIPHNTQVPKKRPNQEHPSTIITVGNEPNQKHFEVIPDPSGNPKKFTVVPYNPYHPNKPCDPKNPKTPQVFEVPKDYPVIQGPDKKFYRLEPDKHNPGQYTFVPVTPLDENHKPSNPHKPDVSFVPIPRKWHLRTTPGIDDWLDLSTTPSPKTTTDNLGTGYSVVTKPEEISEPTTEVDNAETTVGEEELNKEPTEGPMTTEATEQFSTEQPTVPSHTETEVAIELTTAEEIILSTASVTQPTVAGKTESTPAELTGKSEIPQSTVSTTEAPTVATHTPMSVTTEAIQGTTQGAGVSTESETKGQPTGATSGPEEPSKQEPTTSEVSGTGQTASPATSSEAHPRPTAPNVDDVCFKAYDDCKRSRSSYA*

Gene 32 MEMMYTLFFLLFGIVHGQGDGWVLQPDGSYMSYGDGSSGGSYGSTGGSYDGSGGLYGGSSGGSYGEL GSGGLFGGSGGGGFGPGGSYGGFDGGLGGSSGGSQGLPGNGWILQPDGSYLKYEGSGGGGGGGGG GGGSGSDGPPGNGWILQPDGSYMKYDTSGEGNQGSAGSYGGNLPPGFVPPPGYSGNEGGWVLQPD GTYMKTIEQTEPPTEIRYPVNPDYQPDETLESGKKGSPGYSQVVGFGEPGNMNYFKITPGDAENLYDYSV EPVVSRDNPTHSTDTEHGKVISRGTKGEPDYQPILQVGPRNHRRYYKMLPDSSKPSGFNFIPQRLTGWLK KQGGDDTSGEVLEPEESEVTEEANEEIPDEPEIEKKNNDFIICIGRPNRRYLRLKRYSNNPHDYSVEPIGSH DNPDSKPNPNDEEAPEVMTEGTKGNPNYRSIIAVGPKYNRRYVEVLPNSNDPDKYSFAPVDVQVQPGE QATEPNEEDVEYDDLPENPEYKTIPGKKKSYEIFSFGPKNRRVYFRIIRRNPNNPNDIEVFPFKDPEHPDSP PSSDDPSAPEVIQQGDPNDPEDGPVIAVGPKNKKVYFRLGIRKDKVPKFTPVRRGKRIPGKHRLVFTKKTR IWHSRPQKKPLVRKPSAPLKFETSAPVEAATDYSPDEVDVNPENPVEKRPYQIITFGPPKKRIYIKKTPGPS GNPYDYKLEPIGNPEDPNSKPDPKDPDSPEVIETGEPGTPSFAPVVAHGPKQNRKYVIIRPNSENPKDPN QMEFSPAEPVPGSDPKKPRFRILRAAVPVAPKDQFTDNTPKNPNIKTALKPGDKTRYEVISFGPKNRRIYL KKTPGPSNKPDDFKLEPIGNPNDLNSKPDPNDPEAPEVIENGQPGTPDYSPVVAFGPKTRRKYIIVKPSPK NPKEIQFIPAEPEPGSDPKQRRFKPLTKKVLPGLIRPKPPTHPDSPEIKTVEGPHPHQVISFGPENKRIHVKK SPGPSGDPNDYKLEPLENPFDPNSKPDPQDKDAPAVIETGKPGTPNFLPIVSYGPHDNRKYMSFKPIGNT IALSPATLVPGSDPKHPAFNIKTTHPVSPQIPSILKDLHIPTHPLIPPIFNQLPPILNPRGINLLPGLLPVISNLA ELTKPTVQNVEKPNKPSYQVLSFGPLLNRIHIKKTPGPSGKPDDFKLEPIKDPNDPDSKPDPKDPQTPEIIES GKPGTPDHQQVIAFGPKPQRKYVLVKLNPTDSKKLELSPVEPEPGSDNKQPKFRPVVKPVIPTLPLPSLDL HNPVINTVEKPNKPHYQVISFGPSTQRIHIKKTPGPSGKPEDYKLEPLGNPDDPDSKPDPKDPNAPEIVDS GTPGSPDYEPVVAYGPKTARKYVILKPVPLNPKALDLQPAVLEPGSDPKKKKFKVVIQPGISPLLPQILPPF WPKPSIPYLPKPPPITLPLISHGLQNPIVNNIEEPNKPHMQIISFGPILTRVHIKKILGPTGNPNDYKLEPIGN PDDPDSKPDSKDLDAPEIIENGKPGTPDYKPVVAYGPKSLRKYVTLQSRPSNPKEIDLEPAELAPGSDAKK PIFKYLPAIPVFGNKDRNAPEVKTVTDPISKKPVQVISVGPIFKRVHLKKIPGPSGKPNDYKLEPLGNPDDV NSKPDPKDPDSPEIIENGTPGTPDYAPVVAYGKKIRRKYLKIKSNPLQPNDPTKLLFNRVRLLSPKPGEKTP TFSESLIPKFNPVCYKQYQDCNDRS*

Gene 33 MRMKIIILILSLCATTFSSDVTEVADDANPDDLLAQGYVLQPDGSYLKTDETSSTSEQSGTSDQSPDVLKS QGYVQQPDGTWLKTVEDTETTYDDNEYKNTDYSDGILTVQNDPDVGYFKINKGSSDNENDYSVQAVNS PSDPSPINNPGKDDPRVYPQGNQGDPDYHNVIGVGKGKDRTFLKMVPEPSRPSGFKFIPQYISGYRPGD VTNAPEPAPEPAPETSPGSTDDYEEEPNDNPDEPNISDRKGYFIISIGKPNRRFLKLTPSTNNNPHDFSLVP IKSPSEHNSSPDPNDEEAPEVISNENRGSPDYQPVIAVGPPDNRRFTGVVVNPENPKSFQFVVRDQELQP GEAQTEPPEDDIEYEDEPPNINYKVITVGGKKNEVISAGPKNQRFHFRITHKSKNQGDIEIKAINSADNIDS SPDPDDINAPEIVQQGNPEDPNNAPIVGCGPRFRRTYHRLIITKGRARFLPLRRVKIRGRHSFIKRNRRWH YRRGIKKIARRVIKRRPLLIRRRKVKYNKRVVMHRNKLISHNRGGLLIGHKSGHSLGGKGGRLIGGGRAGN GMRHSSRVVIRHHSGSKQNGHGLSLGSRSEGHHSLSIGGRGSSNGGGGGSHHGSHHG*

Gene 34. Charge based adhesive protein (BamH1 and KpnI as cloning site) pQE80L and same as pET. GAGP[(VHKAGGY)(VHKAGGY9]8GWPH6

Gene 35. Scl2 three domains mimic 108 with N and C telopeptide only in the N and C terminal with folden – Should be codon optimized to Pichia and cloned in pPICZalphaA. GPCCQLSYGYDEKSTGGISVPGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPCCGYIPEAPRDGQAYVRKDGEWVLLSTFLSAGFDFSFLPQPPQEKAHDGGRYYRA

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