Sch 6 Supply of Laboratory chemicals, stains, reagents, etc

Supply of Laboratory chemicals stains Reagents & solutions etc

Acetic acid Glacial A.R. Assay 99.9%

Hydrochloric Acid L.R.

Hydrochloric Acid A.R.Colorless assay 35-38%

Nitric Acid A.R.Assay 69-71%

Salicylic Sulphonic Acid A.R.

Sulphuric Acid A.R.Assay 96-98%

Trichloroacetic Acid A.R. Assay >= 99%

Acetone A.R.99 % pure

MAc Conkey’s Agar with 0.15% Bile Salt, with Crystal Violet and Nacl.

Nutrient Agar -Bacteriological Grade

Alcohol Methyl A.R. 99% pure.Acetone free

Ammonium Sulphate A.R.

Chloroform A.R

Dextrose(Anhydrous) A.R.

Diethyl Ether A.R. 99% pure

Formaldehyde 40% A. R.(1 x 25 Ltrs)

Formaldehyde A.R. 40%.(1 x 500 ml)

Isopropyl Alcohol A.R.99-100%

Potassium Iodide A.R. 99.8%

Paraffin Wax cubes 58-60 C (M.P.)

Solution of Benedict’s qualitative

Sodium Citrate A.R.

Sodium Hydroxide A. R. Pellets 98 %

Stain Leishman Liquid A.R.( Ready to use)

Vial containing Thromboplastin with Calcium Ions for Prothrombin Time (PT) Liquid ready to use form, stable reagent, IS index <=1.5

Xylol (extra pure) A. R.

Anti A (Monoclonal) Clear, Sera Titre > 1:256 (Macroscopically) should be printed on vial label and product insert. Avidity <10 seconds

Anti B (Monoclonal) Clear, Sera Titre > 1:256 (Macroscopically) should be printed on vial label and product insert. Avidity <10 seconds

Anti AB (Monoclonal) Clear, Sera Titre > 1:256 (Macroscopically) should be printed on vial label and product insert. Avidity <10 seconds

Anti D IgM (Monoclonal) Clear, Sera Titre > 1:256 (Macroscopically) should be printed on vial label and product insert. Avidity <10 seconds

Chemical Strips for detection of albumin and sugar semi quantitative estimation minimum detectable protein 30mg/100 ml. & Sugar 100mg./100 ml. Colour developed should match the colour chart.

Elisa Kits for HBS Ag.(3rd Generation Kits)- One step sandwich ELISA using monoclonal antibody & conjugate coated wells.

Anti HCV ELISA Kits using Microwells coated with recombinant antigens readablewith automated ELISA reader

R. A. Antigen (containing latex reagent with positive and Negative control, and giving accurate Semiquantative results.)

ASO Latex Test

VDRL Antigen (RPR Card Test) Carbon particle based test with plastic boards. Result to be read macroscopically under a good light Source within 8 minutes asblack aggregates against a white background plus positive and negative controls with stability of one year.

Widal Antigen set. 50 ml. Each. S.typhi -H -50 ml S.typhi - O-50 ml S.Paratyphi - AH - 50 ml S.Paratyphi - BH - 50 ml for tube dilution tests

Media MUELLER HINTON No.2 AGAR cation adjusted with Ca 25 mg/ltr. & Mg 12.5mg/ltr.

Tryptone Soya Broth

Hydrochloric Acid commercial gradefor cleaning purpose.

VDRL Antigen Kits containing stabilised emulsions of cardiolipin lecithin & cholesterol adjusted to required sensitivity. The result should be observableunder Microscope.

ELISA Kit for detection IgM antibody to pathogenic Leptospirosis shelf life to be one year atleast at the time of receipt of Kit.

Rapid test for detecting IgM & IgG antibodies to all serotypes of Dengue virus using recombinant antigen

Malaria antigen detection test capable of detecting pan LDH using monoclonal antibodies & differentiate between Pf & PAN (type of kit Pf/Pv with lateral flow immuno chromatography principle HRP2 for Pf & pLDH for Pv & WHO Prequalified required

ELISA Kit for detection of Ig M antibody to all serotypes of Dengue virus

Rapid test for detecting of IgM antibodies to pathogenic leptospires ( Lateral flow immuno chromatography principle)

Ultra Sensitive HBsAg Elisa Screening test capable of detecting escape mutants.

Dengue NS1 Antigen ELISA shelf life to be 1 yr at least at the time of receipt of kit with either controls or calibrators

Anti HEV igM ELISA

Anti HAV igM ELISA

Total Bilirubin Kit Method-Jendrassik & Grof's End- Point Method (diazotised Sulphanilic Acid) Reagent Type-Liquid Stable/ Lyophilized Minimum Reconstitution stability-On board : 3 days after mixing R1 & R2 Linearity -20mg/dL (CE Mark on Kit)

Direct Bilirubin Kit Method-Jendrassik & Grof's End- Point Method (diazotised Sulphanilic Acid) Reagent Type-Liquid Stable / Lyophilized Minimum Reconstitution stability-On board : 3 days after mixing R1 & R2 Linearity -10mg/dL (CE Mark on Kit)

Rapid Dengue NS1 antigen detection test Lateral flow immuno chromatography principle required.

Blood Urea Nitrogen Method-Initial Rate UV-Kinetic Method Reagent Type-Liquid Stable / Lyophilized Minimum Reconstitution stability-45 days at 2 to 8 degrees Linearity - 250mg/dL (CE Mark on Kit)

Creatinine Method-Initial Rate Alkaline Picrate Method Reagent Type- 2 reagent Liquid Stable / Lyophilized Minimum Reconstitution stability- Liquid Stable Reagents Linearity -30mg/dL (CE Mark on Kit)

Glucose (GOD- POD) Method-Enzymatic GOD-POD End-Point Method Reagent Type- Lyophilized Enzyme / Liquid stable Minimum Reconstitution stability -30 days at 2 to 8 degrees Linearity - 600mg/dL (CE Mark on Kit)

SGOT / Aspartate Transaminase Method-IFCC UV Kinetic Method Reagent Type-Liquid Stable / Lyophilized Minimum Reconstitution stability- 30 days at 2 to 8 degrees Linearity-350 IU/L (CE Mark on Kit)

SGPT / Alanine Transaminase Method-IFCC UV Kinetic Method Reagent Type-Liquid Stable / Lyophilized Minimum Reconstitution stability-30 days at 2 to 8 degrees Linearity -350 IU/L (CE Mark on Kit)

Amylase Kit Method-Kinetic Method using Gal G3 f (alpha) CNP Reagent Type-Liquid Stable / Lyophilized Reagents Minimum Reconstitution stability- 10 days Liquid Stable Reagents Linearity-2000 U/L (CE Mark on Kit)

Vibrio cholerase polyvalent 01 antisera

Vibro cholerae Ogawa antisera

Vibro cholerae inaba antisera

Salmonella Polyvalent antisera (A-G)

Salmonella group 02 antisera

Salmonella group 04 antisera

Salmonella group 09 antisera

Thiosuplphate Citrate Bile salt Sucrose(TCBS) Agar- dehyrated ready to make culture media(Media to be in powder form)supply in HDPE bottle

Xylose Lysine Deoxycholate Agar (XLD agar) supply in HDPE bottle

Amikacin (30 mcg)

Amoxycillin +Clavulanic acid(20 mcg/10mcg)

Ampicillin (10 mcg)

Ampicillin + Sulbactam (10 mcg/ 10 mcg)

Azithomycin ( 15 mcg)

Aztreonam

Bacitracin(0.04U)

Cefaperazone +Sulbactam (75 mcg/ 10 mcg)

Cefepime (30mcg)

Cefoxitin (30mcg)

Ceftazidime (30 mcg)

Ceftazidime + Clavulanic Acid (30 mcg / 10 mcg)

Ceftriaxone (30 mcg)

Chlorampenicol ( 30 mcg)

Ciprofloxacin ( 5 mcg)

Clindamycin ( 2mcg)

Colistin (10 mg )

Colistin MIC (e-test) strips

Erythromycin (15 mcg )

Furazolidone(100 mcg)

Gentamicin (10 mcg)

Gentamycin (120 mcg)

Imipenem (10mcg )

Levofloxacin (5 mcg)

Linezolid (30mcg)

Meropenem (10mcg)

Metronidazole (5 mcg)

Mupirocin (200mcg)

Nalidixic acid ( 30 mcg)

Netilmycin (30mcg)

Nitrofurantoin (300 mcg)

Norfloxacin (10 mcg)

Novobiocin (5 mcg)

Ofloxacin(5 mcg)

Optochin ( 5mcg)

Oxacillin ( 1mcg)

Penicillin G 10U

Piperacillin ( 100 mcg)

Piperacillin + Tazobactam (100mcg/10mcg)

Polymyxin B 300 U

Polymyxin B 50 U

Teicoplanin (30 mcg)

Tetracycline ( 30 mcg)

Tigecycline ( 15 mcg)

Trimethoprim/ Sulphamethoxazole (1.25 mcg/23.75 mcg)

Vancomycin ( 30 mcg)

Vancomycin MIC(e-test) strips

Antisera Clostridium species

Antisera E. Coli O157

Anaerobic Gaspack sachet for 1.5L jar

Spore strips (Bacillus Steothermophilus spores for steam sterilization- 10 spores)

Description : Blood Glucose Test Strip used with glucometer for blood sugar estimation. Being a closed system, the strips have to be of same company as glucometers. Equivalent numbers of Sterile, disposable, auto disabled finger pricking lancets are needed for blood collection by finger prick method. Price to be quoted for supply of Blood glucose test strip & lancet together. (Tenderer has to give initially 200 glucometers free of cost for utilisa tion of glucose strips for EHO Dept.) 1 Free glucometer should be provided by the company for every 2500 glucostrips after issue of P.O.

Rapid immunochromatography Test kit for detection of HBsAg upto (Sensitivity)0.1ng/ml detection limit.WHO Prequalified.

Kovacs reagent

Oxidase reagent (N,N,N,,N,- tetramethyl-p-phenylene diamine dihydrochloride)

Sodium polyanethanol sulphonate (SPS), AR

Triple sugar iron agar

Mueller Hinton broth

Robertson cooked meat medium

Mannitol salt agar

Brain Heart Infusion broth

Nylon flocked swab with medium for viral transport and culture for H1N1 influenza virus isolation

5% sheep blood agar (readymade plates)

Chocolate agar containing 5% sheep blood (readymade plates)

Sheep blood

Crystal violet powder (Practical grade)

Safranine powder (practical grade)

Iodine crystals

Arginine dihydrolase broth

Ornithine decarboxylase broth

Lysine decarboxylase broth

Decarboxylase broth base, Moeller

Urea Agar Base (Christensens)

Phenylalanine agar

Simmons Citrate agar

Fluid thioglycollate medium

Rapid test for detection of screening of antibodies to HCV virus based on the principle of immunochromatography

Rapid test for detection of HIV-1 & HIV-2 Ab’s and p24 antigen from serum , plasma and whole blood. Specificity and sensitivity of the test should be 100% for all sample types Sample volume should be less than 50ul No buffer addition should be required for testing of serum and plasma samples. Test should be WHO prequalified

Ready to use LJ slants

LJ with isoniazid

LJ with rifampicin

LJ with ethambutol

LJ with streptomycin

LJ with PNB 500µm/ml

Nitrate reduction kit

Dubos broth (Tween 80)

Phenopthalein disulphate tripotassium

Peptone water

Sabouraud dextrose Agar

Sabouraud cycloheximide chloramphenicol agar

Yeast nitrogen base Agar (Twin Pack)

Candida chromogenic differential agar base

Rapid detection of Salmonella typhi IgG/IgM Antibody test

Rapid detection of Dengue NS1 Antigen & IgG/IgM Antibody test in same card (Rapid Dengue Duo test)

Cornmeal Agar

Potato Dextrose Agar

Lactophenol cotton blue solution

Rapid test to detect MPT 64 antigen of Mycobacterium tuberculosis complex, the test principle could be immunochromatography or agglutination

Reagent strips urinanalysis 10 parameters They should be able to detect and help in Semi-quantification by visual method of various parameters analysed by developing different colors for different concentrations/quantity of the substrate analysed. The color developed should match with the standard color chart provided. 1. pH- Measure Urine pH values in range Of 5 to 9. There should be different color developed on reagent strip for every 0.5 change in pH. 2. Specific Gravity- Measure Specific gravity of Urine samples in range Of 1.000 to 1.030. There should be different color developed on reagent strip for every 0.5 change in specific gravity. 3. Urobilinogen= Measure Urobilinogen levels as low as 0.4 mg/dl or 7 mol/L. Semi-quantify it as trace, small, moderate, large increase with levels beyond detectable range. 4. Glucose- Detect urine glucose concentration of / near 40 mg/dl (2.2 mmol/L) and above. Semi-quantificatio of urine glucose by visual method should be possible as: Trace(5 mmol/L or 100 mg/dl), +(15mmol/L or 250 mg/dl), ++ (30 mmol/L or 500 mg/dl, +++ (60 mmol/L or 1000 mg/dl), ++++(110 mmol/L or 2000 mg/dl), 5. Bilirubin- Direct urine bilirubin levels of / near 0.5 mg/dl or 9 mol/L and above, Semi quantify it as small, moderate and large increase with level beyond detectable Range. 6. Ketones-Detect urine ketones (acetoacetic acid) levels of /near 5 mg/dl or 0.5 mmol/L and above. Semi quantify it as trace, small, moderate, large increase with level beyond detectable range. 7. Blood- Detect 5 to 10 RBCs/I and above in urine sample. Semi quantity it as trace, small moderate and large hemoglobinuria/hematuria with increase beyond detectable Range. 8. Proteins- Detect urine albumin levels of / near 6 mg/dl and above. Semi Quantify it as trace (<30 mg/dl), +(30 mg/dl), ++(100 mg/dl), +++(300 mg/dl), and ++++(>/=2000 mg/dl), 9. Nitrite- Detect urine nitrite levels of/ near 0.05 mg/dl or 11 mol/L and above. Semi quantify it as trace, small, moderate, large increasewith levels beyond detectable range. 10. Leucocytes- Detect near 10 to 20 WBC/L and above in urine sample. Semi- Quantify it as trace, small, moderate large increase with levels beyond detectable range.

AHGS GEL (COOMBS) TEST CARD For direct Coombs Test, Indirect Coombs test, Indirect Antibody test, Antibody Screening and identification AHGS Gel card should contain Six Microtubes prefilled with gel in suitable buffer containing polyspecific coombs reagent (Anti lgG+Monoclonal Anti C3d. With sheIf life of 12 months and storage temp 4-25 degree C.

DILUENT – 2(LISS) modified Low Ionic Strength Saline Solution for preparation of red cell Suspension

Dengue-Chikungunya RTPCR KIT (1X96 TEST)

Potassium Tellurite Blood Agar

RTPCR kit for detecting COVID-19 virus

RTPCR kit for detecting Dengue virus

RTPCR kit for detecting Influenza A, H1N1, H3N2 & Influenza B virus

Viral RNA extraction kit - manual, spin column based

Viral RNA extraction kit - automated,magnetic bead based compatible with kingfisher flex & Hi Media

Rapid test for Crypto+Giardia+entamoeba

TINSDALE AGAR

ALBUMIN BOVINE SERUM (A. R. grade )

Alkaline phosphatase Liquid stable DEA- PNPP kinetic method Reconstitution stability (if applicable): 10 days Limit of quantification: ≤ 3 IU/L Precision: ≤ 5% Linearity upto 2000U/L

Serum Albumin "Method: Modified BCG Method Reconstitution Stability: 21 days or more Linearity: 9 gm/dL or more Precision: ≤ 5%"

Serum LDL-Cholesterol : "Method: Enzymatic Clearance assay for the in vitro quantitative determination Reconstitution Stability: 21 days or more Linearity: 450 mg/dL or more Precision: ≤ 5%"

CRP Reagentmethod -immuniturbidimetric resconstitution stability 10 days limit of quantification <7mg/l lineraity >200mg/l precision <5%

Sterile Microtitre Assay Plate 96 Well With Lid Clear Round Bottom Non Treated Polypropylene

F-18 FDG (to be delivered to nuclear medicine department due to short half life when ordered )

Bovine Albumin 22% bovine serum albumin for serological application total protein 22% ( g /dl ) conductivity 6-8 10-4 Omms /cm at 25+ /- 1oC storege 2 -8 C /stability 36 months

Colistin Microbroth dilution test (ready to use predrug-coated plates)

Hepatitis B virus e antigen ELISA (HBeAg)

Cefotaxime E strip (0.016- 256 mcg/ml)

Ceftazidime Avibactum E strip (0.016- 256 mcg/ml)

Chloramphenicol E strip (0.016- 256 mcg/ml)

Ciprofloxacin E strip (0.016- 256 mcg/ml)

Class V chemical indicators strips for steam sterilizers

Distilled water

Fosfomycin (200 mcg) antibiotic discs

Gram stain set of 4 bottles ready to use kit

Hepatitis B Core IgM Elisa

Inulin A.R

Levofloxacin E strip (0.016- 256 mcg/ml)

Manual Nucleic acid extraction kit (silica column based)

Meropenem E strip (0.016- 256 mcg/ml)

Multiplex PCR for fever panel (Typhoid,Scrub Typhus, Malaria, Chikungunya)

N-acetyl L-cysteine

Neurovirus pathogen detection kit by PCR (real time)

Penicillin E strip (0.016- 256 mcg/ml)

Poly L Lysine solu tion 100ml bottle 0.1% (w/v) in H2O

Rapid test kits for occult blood testing

RNAse away (500ml bottle)

Rubella Elisa Kit IgM

Rubella RTPCR Kit

Tigecycline E strip (0.016- 256 mcg/ml)

Total Nucleic Acid Automated extraction Kit (1X96 TEST)

Total Nucleic Acid Manual extraction Kit (1X96 TEST)

TPHA -Treponema pallidum haemagglutination assay kit

Viral DNA extraction kit- automated, magnetic, bead based compatible with Kingfisher flex and HI Media

Zika Virus RTPCR Kit

Antibody to HBsAg Elisa Kit ( Quantitative)

Chikungunya IgM Antibody Elisa

Chlamydia antigen Elisa

Absolute Alcohol purity 99.5 %

Charcoal Agar Plates

TODD Hewitt Broth

Absolute alcohol

Alcian Blue

DPX Mountant

HEMATOXYLIN POWDER

HER 2 - BREAST MARKERS FOR IMMUNO HIS

Horse Radish Peroxidase IHC Detection (HRP-DAB) Kit/DAB- 1 step /2 step

ESTROGEN RECEPTER FOR IMMUNO HISTOCHEMISTRY

Ki 67/ MIB-1

Pure paraffin pellets / granules for automate tissue processing congealing point 56-58 degree celsius

PR (PROGESTRON RECPTER FOR IMMUNO HISTOCHEM)

Serum Calcium O-cresolphthalein complexone end point method Single reagent system Reconstitution stability -07 days or more Linearity up to 15mg% Interference of Mg and Fe should be negligible No special treatment should be required for lipemic samples.

Serum Total Cholesterol, Enzymatic End Point Method (CHOD-POD) or smiliar method, Reconstitution stability 21 days or more, Linearity 500 mg/dL or more, Precision <= 5%

Serum HDL Cholesterol, Enzymatic clearance assay for the In Vitro Quantitative Determination, Reconstitutional stability 21 days more, Linearity 150 mg/dL or more, Precision <=5%

Serum Lactate Method: Lactate oxidase Reconstitution stability (if applicable): 10 days Limit of quantification: ≤ 4.5 mg/dL Linearity: ≥ 135 mg/dL Precision: ≤ 5%

Serum Lipase Method: UV Kinetic method Reconstitution stability (if applicable): 10 days Limit of quantification: ≤ 3 IU/L Linearity: ≥ 250 IU/L Precision: ≤ 5%

Serum Phosphorus U.V. end point method Single reagent, ready to use Linearity up to 20 mg% or more Long shelf life Pack size should be 30 – 50 ml

Serum Triglyceride Method: GPO-PAP Method Reconstitution Stability: 21 days or more Linearity: 1000 mg/dL or more Precision: ≤ 5%

Sr Magnesium "Method: Xylidine blue Reconstitution stability (if applicable): 10 days Limit of quantification: ≤ 0.2 mg/dL Linearity: ≥ 5.5 mg/dL Precision: ≤ 5%"

Serum Uric acid kit Uricase PAP method Reconstitution stability (if applicable): 10 days Limit of quantification: ≤ 3 IU/L Precision: ≤ 5% Linearity up to 20mg/dl

Bicarbonate formalin emen diluting solution

DISTILLED WATER (5LTR) TDS < 1 %

FIELD STAIN A

FIELD STAIN B

G6PD KIT quanlitative

Ketone & glucose strips

Genomic Dna Extraction Kit for multiple sample for viral bacterial & fungal for automated magnetic bead based compatible with open system

Homocysteine with 5 point calibrator and 3 level control Linearity 50 micro mol/ Litre

SB C18 HPLC Column 250x4.6mm,5micron

HIV ELISA GENERATION 4TH KIT for HIV Ag& Ab 1& 2 viruses for detection of HIV-1 P24 Antigen & Antibodies to HIV-1 and HIV -2 in Human Serum /Plasma

Haem test