Chemicals

Diluent for DNA extraction Size 500ml A diluent used in DNA extraction is typically TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~8.0) or nuclease-free water, used to dissolve and dilute extracted DNA. Tris maintains a stable pH, while EDTA chelates divalent cations (e.g., Mg²⁺), thereby inhibiting DNase activity and protecting DNA from degradation. The diluent must be sterile and free of nucleases to ensure the integrity of DNA for downstream applications such as PCR, sequencing, and cloning.

Luria–Bertani medium Size 500g Luria–Bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as Escherichia coli and Agrobacterium tumefaciens. It contains tryptone (10 g/L), yeast extract (5 g/L), and sodium chloride (10 g/L), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. LB medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. For solid media, agar (15g/L) is added.

Chloroform (High Analytical Reagent grade) Size 2.5L Chloroform (High Analytical Reagent grade) is a high-purity organic solvent used in DNA extraction, particularly in phenol–chloroform methods, to separate proteins and other contaminants from nucleic acids. It enhances phase separation by forming a distinct organic layer, allowing DNA to remain in the aqueous phase. Due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions.

DEPC Size 25ml DEPC is a chemical used to inactivate RNases in water and laboratory solutions, making them suitable for RNA work. It modifies histidine residues in proteins, thereby deactivating RNase enzymes that can degrade RNA. DEPC-treated solutions are typically incubated and then autoclaved to remove residual DEPC, ensuring they are safe for use in downstream applications such as RNA extraction and cDNA synthesis.

TB Green Master Mix Size 200Rnx TB Green Master Mix is a ready-to-use reagent used in real-time PCR (qPCR) for DNA amplification and detection. It contains TB Green dye (a double-stranded DNA-binding fluorescent dye), DNA polymerase, dNTPs, Mg²⁺, and reaction buffer. The dye binds to amplified double-stranded DNA and emits fluorescence, allowing real-time monitoring of PCR amplification. It is commonly used for gene expression analysis and quantification of DNA.

DNA loading dye Size 10 Inch DNA loading dye is a solution added to DNA samples before agarose gel electrophoresis. It contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of DNA migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. It may also include EDTA to protect DNA from nuclease degradation.

Metaloop A metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. It is sterilized by heating in a flame until red hot before and after use to prevent contamination. It is commonly used in microbiology for streaking plates and handling bacterial cultures.