Supply of Chemicals

Purchase of chemicals and reagents

RAW 264.7 Cell

INS-1 832/3 Rat Insulinoma Cell Line

Dulbecco's Modified Eagle's Medium (DMEM)

DMEM, high glucose, GlutaMAX™ Supplement

RPMI Medium 1640 (1X), liquid; with L-glutamine

RPMI 1640 Medium, no glucose

Fetal Bovine Serum, qualified, USDA-approved regions

Penicillin-Streptomycin (10,000 U/mL)

HEPES sodium salt

PYRUVIC ACID, Sodium Salt, Reagent Grade

2-Mercaptoethanol

Trypsin-EDTA (0.05%), phenol red

Normal Goat Serum (10%)

Normal Goat Serum (10%)

Syntaxin 2 shRNA Plasmid (m)

Mouse STX2 shRNA silencing Adenovirus/Ad-m-STX2-shRNA-mCherry/RFP/CFP

Lipofectamine™ 2000 Transfection Reagent

Opti-MEM™ I Reduced Serum Medium, no phenol red

Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit

Premo™ Autophagy Sensor LC3B-GFP (BacMam 2.0)

Zymosan A BioParticles, Alexa Fluor 594-conjugated

Zymosan A BioParticles opsonizing reagent

pHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis

Fc OxyBURST™ Green Assay Reagent

Diabetes Associated Peptide Amide human

ProLong™ Diamond AntifadeMountant with DAPI

LC3A/B (D3U4C) XP® Rabbit mAb #12741

Atg16L1 (D6D5) Rabbit mAb #8089

PI3 Kinase Class III (D9A5) Rabbit mAb #4263 (Rabbit)

Beclin-1 Complex Antibody Sampler Kit #59187

Anti-mouse IgG, HRP-linked Antibody #7076

Anti-rabbit IgG, HRP-linked Antibody #7074

Normal Rabbit IgG #2729

Super Signal™ West Pico PLUS Chemiluminescent Substrate

Protein A - Sepharose™ 4B

Syntaxin 2 Polyclonal Antibody (Rabbit Polyclonal)

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L)

Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat SrProt)

Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L)

Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat SrProt)

Alexa Fluor® 647 AffiniPure Goat Anti-Rabbit IgG (H+L)

Alexa Fluor® 647 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat SrProt)

Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L)

Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, SwSrProt)

Alexa Fluor® 594 AffiniPure Goat Anti-Mouse IgG (H+L)

Alexa Fluor® 594 AffiniPure Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, SwSrProt)

Alexa Fluor® 647 AffiniPure Goat Anti-Mouse IgG (H+L)

Alexa Fluor® 647 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, SwSrProt)

D-GLUCOSE, Anhydrous, Reagent Grade

Dextrose extrapure AR, ACS

Sodium Chloride for molecular biology, 99.9%

Potassium Chloride (KCl)

Disodium phosphate (Na2HPO4)

Monopotassium phosphate (KH2PO4)

Potassium hydroxide (KOH)

Sodium Hydroxide 1N Solution

TRITON X-100, Biotechnology Grade

Protease Inhibitor Cocktail

Halt™ Protease Inhibitor Cocktail, EDTA-free (100X)

SODIUM DODECYL SULFATE Reagent Grade : Purity: >99%

Sodium Lauryl Sulphateextrapure AR, ACS, 99%

Trizma® base

Tris Buffer Superior extrapure AR, ACS, 99.9%

GLYCINE, Reagent Grade, min 99% Suitable for Electrophoresis

Glycine extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5%

EDTA Disodium Salt 0.5M Solution

Glycerol for molecular biology, 99.5%

Methanol

Hydrochloric Acid 1N Aq. Solution

Acrylamide/Bis-acrylamide, 30% solution

ACRYLAMIDE/BIS-ACRYLAMIDE, 30% Solution 19:1

TEMED, Electrophoresis Grade

Sodium palmitate

Sodium oleate

ALBUMIN, BOVINE SERUM , Heat Shock Isolation, Fraction V. Min. 98%

Albumin Bovine (pH 5.2) fraction V (Bovine Serum Albumin, BSA), 98%

Sodium hypochlorite

Propionaldehyde

Sodium fluoride

Sodium thiocyanate

Ammonium hydroxide

2-4-Dichlorophenoxyacetic acid

Diethylene Glycol Monoethyl Ether

Low melting point Agarose

Agarose

Sodium dodecyl sulfate (SDS)

Triton X-100

Ethidium Bromide

Sodium Carbonate Anhydrous extrapure AR, 99.9%

L-Alanine extrapure CHR, 99%

Trichloroacetic Acid extrapure, 99%

Glutathione Reduced (GSH) ExiPlus, 99%

Tetrasodium Pyrophosphate Decahydrate (TSPP Decahyd) extrapure AR, 99%

PhenazoniumMethosulphate (PMS) extrapure, 99%

Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) extrapure AR, 99%

β-Nicotinamide Adenine Dinucleotide Disodium Salt (Reduced) (β-NADH.Na2, DPNH.Na2) extrapure, 98%

Acetic Acid Glacial extrapure AR, 99.9%

n-Butyl Alcohol pure, 99%

Potassium Phosphate Dibasic Anhydrous pure, 99%

Potassium Dihydrogen Orthophosphate extrapure AR, 99.5%

tert-Butyl hydroperoxide

Bisbenzimide (Hoechst 33258)

L-Glutathione reduced

1-CHLORO-2,4-DINITROBENZENE

2-Thiobarbituric acid, Hi-AR™

5,5’-Dithiobis(2-nitrobenzoic acid)

Sodium Bicarbonate

Sodium hypochlorite (4% w/v solution)

Brij-30 (Polyethylene Glycol Dodecyl Ether, Brij-L4)

Sodium Lauryl Ether Sulphate (SLES, AES), 28-30%

Zinc Chloride pure, 97%

Chromium (VI) Oxide pure (Chromium Trioxide), 99%

Nickel (II) Oxide pure, 77% Ni

Lead oxide, red

Mercuric Chloride extrapure AR, ACS, ExiPlus, 98%

Sodium Lauryl Sulphateextrapure AR, ACS, 99%

Methylparaben

Ethylparaben

PFOA-Perfluorooctanoic acid

Glutathione Reductase

Anthracene

Tris Buffer

Napthalene

Anthracene

Phenanthrene

Pyrene

Acenaphthene

Fluorene

Sodium dodecylbenzene sulfonate

Transformation aid kit

Plasmid Isolation Mini Kit

Agarose Gel Extraction Kit

Genomic DNA Isolation Kit

dNTP Mix for PCR

Pfu DNA Polymerase