Rate Contract for Consumables and Media for IVF lab Kalsi

Medical grade 47 liters water capacity Gas Refilling Carbon Dioxide (CO2) 99.97 Pure

IVF Grade 47 liters water capacity Mix Gas ( 6.5 % CO2 + 5 % O2 + N2)

N2 Gas 47 liters water capacity

Intra cytoplasmic sperm injection dish, made from Polystyrene & IVF Grade

Disinfectant for CO2 & Laminar surface MEA and HSSA tested, odorless, alcohol-free, No VOC release & CE marked

Disinfectant for Laboratory surface MEA and HSSA tested odorless, alcohol-free, No VOC release & CE marked

Disinfectant for Laboratory surface MEA and HSSA tested odorless, alcohol-free, No VOC release, CE marked with bottle with spray

Humidification bottle with filter to be used in planner bench top incubator

Light yellow ID Rod for straws 25 mm or more size used for embryo loading and transfer and freezing of bovine embryos.

Nitrile Gloves 7.5 inch packed CE compliant, latex free, powder-free

Sterile OPU Recovery Media for Ovum Pick Up in Bovines

Sterile 90 X 90 mm square Petri Dish with grid for oocyte and embryo searching

Follicle stimulating hormone (FSH) as a lyophilized powder. Highly purified porcine pituitary gland extract. Provided with Follicle Stimulating Hormone diluent Bacteriostatic Sodium Chloride solution

Inj lignocaine with adrenaline. Each ml should contain 21.3 mg lignocaine HCl and 0.005 mg adrenaline potassium equivalent

Inj Cloprostenol Sodium. Each ml should contains 250 mcg of cloprostenol

Inj. GnRH analog. Each ml should contain 0.004 mg buserelin

Hypodermic Sterile Needle 18G, one and half inch long

Hypodermic Sterile Needle 24 G, one and Half inch long

Sterile 1.5 ml micro centrifuge Screw cap tube capable of 20,000xg centrifugal force or more & individually packed

Sterile 2 ml micro centrifuge Screw cap tube capable of 20,000xg centrifugal force or more & individually packed

Sterile ET Plugging rod 13 mm for embryo straw

Aluminum can 2 x 13 mm for holding 13 mm plastic goblet for embryo storage

Plastic Goblet 13 mm for keeping frozen embryo straws

Plastic Goblet 35 mm for semen and embryo storage

Disposable gloves, Length 920 mm - Thickness 24 microns, Red color. To be used for palpation of bovines and embryo transfer process.

Sterile Disposable syringe 1 ml with no rubber, non pryogenic & non toxic

Sterile Disposable syringe 2 ml with no rubber, non pryogenic & non toxic

Sterile Disposable syringe 5 ml with no rubber, non pryogenic & non toxic

Sterile Disposable syringe 10 ml with no rubber, non pryogenic & non toxic

Sterile Disposable syringe 20 ml with no rubber, non pryogenic & non toxic

Sterile Disposable syringe 50 ml with no rubber, not made with natural rubber latex , Silicon oil free, non pryogenic & non toxic

Sterile Oocyte maturation media for bovine use. Not exceeding one month from date of manufacturing date on date of supply.

TMC 199 500 ml Sigma (specification as mentioned in bid)

DMEM F-12 500 ml Sigma (specification as mentioned in bid)

DPBS+ive 500 ml Sigma (specification as mentioned in bid)

DPBS-ive 500 ml Sigma (specification as mentioned in bid)

D-manitol-0.3 molar Sigma (specification as mentioned in bid)

Magnisium sulphate-0.1mili molar Sigma (specification as mentioned in bid)

Calcium chloride dehydrate 0.05 mili molar Sigma (specification as mentioned in bid)

Poly vinyl alcohol 1mg/mal (PVA) Sigma (specification as mentioned in bid)

FBS (serum) Sigma (specification as mentioned in bid)

BSA fatty acid free sigma (specification as mentioned in bid)

Sodium pyruvate sigma (specification as mentioned in bid)

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture nF-12 Ham (specification as mentioned in bid)

Estradiol 17-β Sigma (specification as mentioned in bid)

Hoechst 33342 Sigma (specification as mentioned in bid)

Hyaluronidase Sigma (specification as mentioned in bid)

Protease Pronase E Sigma (specification as mentioned in bid)

Cytochalasin B Sigma (specification as mentioned in bid)

Phytohemagglutinin PHA Sigma (specification as mentioned in bid)

Calcium ionophore Sigma (specification as mentioned in bid)

6- Dimethyl Amino Purine (DMAP) Sigma (specification as mentioned in bid)

Trypsin Sigma (specification as mentioned in bid)

DMSO Sigma (specification as mentioned in bid)

Tween 20 Sigma (specification as mentioned in bid)

Ionomycin Sigma (specification as mentioned in bid)

Collagenase Sigma (specification as mentioned in bid)

Penicillin Sigma (specification as mentioned in bid)

Gentamicin sulphate sigma (specification as mentioned in bid)

L-glutamine sigma (specification as mentioned in bid)

PBS+Anitimtcotic 100 X Gibco (specification as mentioned in bid)

PBS Sigma (specification as mentioned in bid)

Karyotyping Media (specification as mentioned in bid)

Self-venting labelled bottle (Bottle displays the CAS number, has a leak-proof one-piece closure, an angled neck for a uniform stream, and a wide mouth for easy filling. Made of LDPE/PP. Includes isopropanol, ethanol, methanol, and water.) - Tarsons

Self-venting labelled bottle (Bottle displays the CAS number, has a leak-proof one-piece closure, an angled neck for a uniform stream, and a wide mouth for easy filling. Made of LDPE/PP. Includes isopropanol, ethanol, methanol, and water.) - Tarsons

Self-venting labelled bottle (Bottle displays the CAS number, has a leak-proof one-piece closure, an angled neck for a uniform stream, and a wide mouth for easy filling. Made of LDPE/PP. Includes isopropanol, ethanol, methanol, and water.) - Tarsons

Wash Bottle (Soft and easy to squeeze. Wide mouth for easier filling. Closure and stem molded in one piece. Leakproof. The dispensing tip can be cut back to increase flow. Material conforming to US FDA 21 CFR.(250 mL) Tarsons

Hand protective silicone grip (Silicone grip for safely handling hot agar flask.) Tarson

Rack for micro tube (To handling 0.5 mL) Tarson

Rack for micro tube (To handling 1.5 mL microcentrifuge tubes) Tarson

Microtube box 0.2 mL (Tarson)

Microtube box 0.5 mL (Tarson)

Flip-flop microtube rack, Rotating rack with 96 slots for securely holding 0.2 mL tubes in an upright position.

Somersault rack

Somersault rack

Magicool PCR mini cooler Cold pack for 96 well plate

Drying Rack, with drain hole Washed glassware drying

minus 20 PCR Mini Cooler Aluminium block for 0.2 mL tube

Tough Spots Assorted Colors, 3/8” tough spots, 5-assorted colors

PCR Workstation Rack, Polypropylene rack for 0.2 ml PCR tubes strips and plates. This rack is stackable with lid.

Sharp Container 1.5L

Sharp Container 5L

Biohazard Waste container To discard EtBr containing gel

UV Safety Goggles To visualize gel on UV trans illuminator

Slide Box Karyotyping slides storage

Microscopic Slide File Karyotyping slide easy identification and reading

Nuclease free water, for molecular biology, Nuclease-Free and Protease-Free Water,suitable for PCR work (Sigma-aldrich)

FastDigestTaqI Rapid, high-purity, heat-inactivatable restriction enzyme. (Thermo scientific)

FastDigestEco88I High-purity restriction enzyme (10 U/μL), heat-inactivatable, optimal for DNA digestion. (Thermo scientific)

FastDigestEco47I High-purity restriction enzyme (10 U/μL), fast digestion, heat-inactivatable, reliable DNA cleavage. )Thermo scientific)

DreamTaqgreen PCR Master Mixes (2X) Optimized, high-fidelity, ready-to-use mix for efficient, robust PCR amplification. (Thermo scientific)

Ethidium Bromide Solution, molecular grade free of DNase, RNase and protease activity, concentration of 10mg/ml.Nucleic acid band visualization (Promega)

TopVision Agarose High-purity, low-background, rapid-gelling agarose for sharp DNA/RNA electrophoresis resolution. (Thermo scientific)

TAE Buffer (Tris-acetate-EDTA) (50X) High-purity Tris-acetate-EDTA solution for reliable DNA/RNA electrophoresis and nucleic acid stability. (Thermo scientific)

DL-Dithiothreitol(DTT) Reducing agent, prevents oxidation, stabilizes proteins, ideal for biochemical and molecular biology (Sigma-aldrich)

TCEP 1M solution Stabilizes proteins, reduces disulfide bonds, ideal for biochemical and molecular biology (UBPBio)

QIAamp Circulating Nucleic Acid Kit solates high-quality cfDNA/cfRNA from plasma/serum for reliable molecular analysis (Qiagen)

HiKaryoXL nutrient mixture f-10 Ham RPMI Medium w/ L-Glutamine, FBS, PHA-P, Penicillin, Streptomycin and Sodium bicarbonate (HiMedia)

Gurr buffer tablet Use for the preparation of a hypotonic solution to enhance the visualization of chromosomes. (Gibco)

Mountant (Eukitt® Quick-hardening mounting medium) Enhances clarity, Embedding and preserving specimens on microscope slides. (Sigma)

PureLink™ Genomic DNA Mini Kit Efficient, high-purity genomic DNA isolation from blood. (Invitrogen)

Isopropanol (5 litres) ≥99.7%, FCC, FG (Sigma)

BamHI-HF Genomic DNA digestion (New England BioLab)

Sterile cotton swab With wooden stick, individually packed. (HiMedia)

Petri dish 100mm, Borosil Borosilicate Glass Size 100 mm (Borosil Scientific)

Petri dish 150mm, Borosil Borosilicate Glass Size 150 mm (Borosil Scientific)

Petri dish 200mm, Borosil Borosilicate Glass Size 200 mm (Borosil Scientific)

Petri dish 90mm, Sterile 90 mm petri plate (HiMedia)

L-shaped spreader, Sterile L Shaped Spreader Individual Packing(Tarsons)

Liquid Nutrient agar, Nutrient agar media(HiMedia)

Mueller-Hinton Agar, For determination of susceptibility of microorganisms to antimicrobial agents.( HiMedia)

Mueller-Hinton Broth, To determine the susceptibility of bacteria to Sulphonamides by the tube dilution method. ( HiMedia)

Glass tube, Borosilicate glass tubes 16 x 150 mm (Borosil Scientific)

Inoculating loop, Nichrome Loop-D-4(HiMedia)

Inoculating loop handle, Metal Loops Holder(HiMedia)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Ampicillin) HiMedia

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Penicillin) HiMedia

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter) (Neomycin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Oxacillin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Amoxicillin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Gentamicin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Tetracyclin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter) (Quinolone- Enrofloxacin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter) (Cephalosporins-Cephalexin)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter) ( Cephalosporins- Ceftriaxone)

Antibiotic Sensitivity disc (Filter paper discs of 6 mm diameter)( Quinolone- Ciprofloxacin)

Sterile 10 uL micropipetts filter tips, ep Dualfilter T.I.P.S. SealMax PCR clean and sterile colorless tips ( Eppendorf)

Sterile 20 uL micropipetts filter tips, ep Dualfilter T.I.P.S. SealMax PCR clean and sterile colorless tips)

Sterile 200 uL micropipetts filter tips(ep Dualfilter T.I.P.S. SealMax PCR clean and sterile colorless tips (Eppendorf)

Sterile 1000 uL micropipetts filter tips, ep Dualfilter T.I.P.S. SealMax PCR clean and sterile colorless tips (Eppendorf)

Antibiotic disc dispenser(Oxoid Antimicrobial Susceptibility Disc Dispenser 6 Oxoid Disc Cartridges 90mm diameter lates (Thermo Scientific)

Turn table, To rotate the 90mm bacterial culture dish (HiMedia)

Reversible Rack, Rack holds 80 number of1.5ml or 2ml. tubes, cryovials, etc. Whereas the reverse side holds 60 numbers of 0.5 ml tubes. (Tarsons)

Combilock Rack, Interlocking racks can accommodate different sizes of tubes. Holds four 50 ml tubes, twelve 15 ml tubes, thirty two 1.5 ml micro tubes or thirty two 0.5 ml micro tubes (Tarsons)

Test Tube Stand, Microbiology work (Tarsons)

Cryo Rack - 50 Places, To holding the cryovials (Tarsons)

CRYOCHILL™ Coder, Coder fit external vial cap Red (Tarsons)

CRYOCHILL™ Coder, Coder fit external vial cap Yellow (Tarsons)

CRYOCHILL™ Coder, Coder fit external vial cap Green (Tarsons)

Card Board Cryo Box, To store DNA and cryovials in ultra deep freezer (Tarsons)

Storage Boxes, SAN / LDPE(1000 mL storage boxes to store 0.5 mL and 1.5 mL tube inside the lab (Tarsons)

Cryobox for 2D Coded CRYOCHILL™ Vial, PC, 1.8 mL storage rack to store cryovial with visible coder (Tarsons)

Indicator Tape for Steam Autoclave, Indication of completion of normal sterilization (Tarsons)

Primers for Sequencing, HPLC purified (IDT)