E - Tender for the procurement of Consumables Glassware for PCR Lab in Microbiology Department

Isopropyl alcohol: molecular-grade

DNA away solution – surface decontaminant to eliminate unwanted DNA and DNA ase from glassware and plasticware without affecting subsequent DNA specimens. 250ml bottle

Lysozyme - Molecular grade 5gm

Molecular - Proteinase K - Molecular grade 500mg

Molecular - Tris - acetate- EDTA (TAE) 50X , must be tested for absence of DNAases, RNAase and proteases

Molecular -DNA ladder 100bp

Molecular -DNA ladder 1kb

Molecular -DNA ladder phi X 174

Ethanol Molecular grade for PCR DNA

PCR - Customised Oligonucleotides (sequence will be provided) primers 10nm to 1umol concentration, HPLC grade minimum 5.0OD

PCR - Customised Oligonucleotides probes labelled with FAM (sequence will be provided), minimum 5.0OD

PCR - Customised Oligonucleotides probes labelled with VIC/Cy5/HEX (sequence will be provided), minimum 5.0OD

Cryo vials 2ml. autoclavable

Cryo vial rack

Disposable plastic forceps

Discarding jar

Cryo labels, should withstand 100-1500C;boiling water bath and autoclaving. 1.30” x 0.5”

Disposable plastic pipette 3ml

Cool box for PCR

RNase P

Tube rack 1.5 ml

PCR Tube rack

PCR tube with flip cap 200ul

Test tube rack holder for 25mm test tubes

Aerosol barrier tips , presterilised, 100-1000ul

Aerosol barrier tips , presterilised, 20-200ul

Aerosol barrier tips , presterilised, 0.1-2ul

Aerosol barrier tips , presterilised, 2-20ul

Microcentrifuge tubes, 1.5-1.8ml, presterilised, screw capped

Microcentrifuge tubes, 0.5ml, presterilised, screw capped

Eppendorf tube, 1.7ml

PCR Plates low profile 0.2 ml, 96 well

Cryo vial marker

Labels-specimen type for labeling and identifying specimens, 2x1” with details for name, date, age/sex, hospital no.

PCR tubes 0.1ml

PCR tubes 0.2ml

Screw capped O-ring centrifuge tubes 2ml, presterilized

Sealer tapes: pressured sensitive film to meet optical standards for PCR real time , should be suitable for -700C to 1000C, RNase/DNase/Human DNA free

Whatman filter paper no.1

Haz Mat Sorbent pads. Should be suitable for wipe up smaller chemical spills or absorb liquids on laboratory bench tops. Should be suitable to wipe acids and water.

Molecular - Flipstrip rack- Reversible rack with lid-one side fits 1.2-1.5ml and 12-8 strips PCR tube; 1 Side fits 96 .5 ml PCR tubes Assorted"A"- 3each

PCR tube workstation alphabetical and code indexed, 60 place for 1.5ml and 0.5ml PCR tubes, dimensions 205x72x26

Rack for 96 PCR tubes 0.2ml with cover

Rack for Microtubes-Material RPP 20 places, 1.5-1.8ml,

Rack for storage vials size 3-5ml-Material PP/PC 150 mm

Elution tube for nucleic acid extraction for PCR

Elution tube tip for nucleic acid extraction for PCR

12 tip comb for nucleic acid extraction for PCR

96 well deep plate

Embee Diagnostics Pvt Ltd

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