Supply and installation of -800C Freezer, BOD Shaker-incubator, Biosafety Cabinet, Gel Electrophoresis and Western Blotting Apparatuses, Phase-contrast Microscope with camera under BOOST of Acharya Prafulla Chandra College

INSTRUMENT

freezer CONSTRUCTION • The freezer must be constructed using 1” thick vacuum panel insulation in conjunction with environmentally-friendly water blown foam • Door gasket must provide 7 independent insulation zones along with 4 points of contact to ensure sample security. • Freezer shall be painted with high-impact, scratch resistant powder coat finished interior and exterior to ensure long term viability and enhanced temperature uniformity. • To reduce condensation, the perimeter heater shall be on the door side not on the cabinet side to limit heat introduction into the sample storage area. • The thermal breaker shall be made of plastic to limit heat leakage into the cabinet • Door latch allows one-handed opening and closing. Handle must include door key lock as well as padlock provision for added security. • Freezer shall have 4 internal storage compartments with a minimum of 4 polystyrene insulated inner doors to ensure sample security. Inner doors should have no latches or external magnets and must be removable for easy cleaning without the use of tools. • Freezer shall have an automatic heated pressure equalization port which allows for rapid re-entry to cabinet. • Freezer shall have two-1 inch access ports as standard. • Freezer shall have a RS485 output, dry contacts and 4-20mA output for remote monitoring purposes. • Freezer must have capability of being cloud connected for remote system monitoring. • Freezer door must open at least 180 degrees for easy sample access. CAPACITY • Freezer shall hold 300 2” boxes or 30000 2ml Vials, not less than 400 lt CONTROLS • Freezer must incorporate H-Drive Information Center (HIC) • Freezer shall incorporate set point security • Freezer interface shall incorporate icons to advise users of alarm status for warm or cold excursions, door ajar, or power failure, to communicate service warnings, health status icon. • Freezer interface shall have warm alarm test function • Freezer interface shall allow the user to adjust the operating and alarm set points • Freezer shall record temperature excursions including actual temperature, warmest temperature and coldest temperature. • Freezer must recognize if line voltage and frequency does not match freezer specification and alert user. Empty freezer shall recover from door opening to -75°C set point in under 17 minutes. Supplier must provide test data verify freezer performance. Empty freezer should not warm to -50°C from -80°C set point in under 241 minutes during a power failure in a 20°C room Sound • Freezers must maintain a sound level no louder than: 51 dB(A) REFRIGERATION SYSTEM • Freezer shall use only natural, commercially available refrigerants (Hydrocarbon) with no special blends required. REGULATORY • Freezer must be built to and contain the registration mark for UL, cUL, and CE standards for safety and performance. Suitable 5 KVA voltage stabilizer need to be supply. WARRANTY: Must be 5 years from factory on overall instrument. Bidders must submit CE certificate of coated model including Product Part Number. Bidders must have minimum 100 installations of quoted models in Easter region. Quoted model must be available in Company website.

BOD Incubator with Shaker(6CFT) Make: Apogeetech Specifications: Double walled with inner chamber of stainless steel & outer wall of M.S. Sheet duly Powder coated. Temperature will be controlled by Digital controller with air circulation. Temperature range : 5ºC to maximum 60ºC with accuracy range of ± 1ºC. Chamber Size: 6 CFT Shaker Speed : 20-180 RPM. Speed control by easy movement variable auto transformer Capacity : Suitable No. of 250/500 ml flasks Suitable number of adjustable shelf and RPM counter is provided Temperature range between which will be controlled by means of a Digital Temperature Controller in conjunction with an electronic relay.

Item name:- DEWINTER ‘CROWN-PLUS’ TRINOCULAR TURRET PHASE CONTRAST MICROSCOPE‘ DIGI-530’ HDMI CAMERA ATTACHMENT AND SOFTWARE Optical System : Infinity color corrected optical system Viewing Head : 30°inclined, gemel Binocular head, interpupillary Distance 47mm-75mm. Third port Camera attachment. Eyepieces : High eye point wide field plan Eyepiece 10X/22mm, with Adjustable diopter and eyeguard. CT Eyepiece for Phase Contrast application. Phase Cum : Infinity Plan PH-10X(N.A.0.25W.D.12.1mm) Bright Field : Infinity Plan PH-20X(N.A.0.40W.D.1.5mm) Objectives : Infinity Plan PH-40X(SL)(N.A.0.65W.D.0.36mm) Colour Coded : Infinity Plan PH-100XOil(SL)(N.A.1.25W.D.0.18mm) • Infinity Plan-4x(N.A.0.10W.D.11.9mm) Nosepiece :Reversed angle Quintuple nosepiece Focusing Mechanism :low position coaxial focusing mechanism, coarse range: 25mm, fine precision: 0.002mm, with tension adjustment and upper limit. Mechanical Stage :210mmX140mm double-layer Reckless composite mechanical moving stage, double slide clamps, moving range: 78mmx 60mm; accuracy: 0.2mm (X-axis single rail driving Reckless) without rack protruding structure, stage surface with wear resistant and corrosion resistant treatment. Transmitted : N.A. 1.25 compound eye illumination with combination of three lens for Illumination System fatigue free, soft illumination, instead of single lens condenser. Condenser preset center, adaptive 100V-240V having anti mold power cord and switching power supply, single high brightness 3W LED (cold color temperature), light intensity can be continuously Adjusted. Lamp life 60,000 hrs. of sustainable lighting. Sub-Stage : Abbe condenser N.A. 1.25 with Aspheric lens and marked Condenser for phase iris diaphragm relevant Turret phase attachment with Contrast observation respective phase annular for 10x, 20x, 40x & 100x objectives and one position for bright field observation fitted in metal made rack and pinion movement by left hand placed knob. Power Unit : Plug in internal SMPS power supply unit 100/240VAC, 50/60Hz. Output 5VDC 500mA. Plug Socket fitted into the microscope rear main frame with on/offswitch. Accessories : Dust Cover, Alen Key, Power Cord and Green Filter for Phase etc Specifications: Camera Sensor : 1/2.8"(5.38mmx3.02mm)FULLHD Resolution :2592 x1944(8Bit) Mode : Dual ( Color / Mono ) Dynamic Resolution : 1920 x 1080p @ 30fps. Pixel Size : 2.8 x 2.8μm Output :HDMI,USB,SDCard Framerate :60fps at1080P for LED or Android TV Memory card Support :TF card(Max32GB) Snap Format :JPEG Video Format :AVI Exposure :Auto/Manual White Balance :Auto/Manual Cross Line :Horizontal 4 lines,Vertical 4 lines Freeze/Unfreeze : Available Cross Line Move/Color : Available High Dynamic Range : Support Digital Noise Reduction : Support Camera Mount : C or CS Mount Power Supply :DC12V Light Frequency : 50HZ or 60HZ Working Temperature : 0-50 Degree Camera Size : 78x 70x 92mm. Weight :200g C-Mount Reduction Lens :0.50x

Gel Electrophoresis and Western Blotting Apparatuses 1. A horizontal electrophoresis system should be able to run the gel size of 7 x 10cm & 7 X 7 cm, and 7 x 10 cm the gel tray should be supplied along with the Gel tank with safety lid. 2.The supplied gel trays should be UV proof and the trays can be directly kept on the UV Transilluminator and should have a integrated fluorescent ruler in the tray. The ruler should get illuminated on exposure to UV Light for easy and safe calculation of the band movements. 3.A system should include tape free gel casting module for leak free operations. 4.A system should include two 1.5mm combs, 8- & 15-well fixed height combs each. 5.A system should have the option for adjustable height combs with comb holders. 6.Migration rate of Bromophenol Blue dye should be similar to 4.5cm/hr(at 75 V). 7.A system should have a lid with the safety banana Jacks, which breaks the circuit when the lid is running. 8.Should be provided with a bubble leveler for even gel casting. 9.The gel caster should have 3 height adjustable screws for balancing the uneven platforms for uniform gel casting. 10.The electrodes should be color coded to remove the confusion of wrong orientation. 11.The Lid should have a safety option so that the lid cannot be closed in the wrong orientation. 12.The Lid should have a integrated cables to connect it to the power pack directly. 13.A system should be capable to run precast ready agarose gels and Hand Cast gels. Power Supply Basic- Programmable power supply should be capable to operate four electrophoresis units simultaneously for four identical runs with graphic LED display. The output range should be 10-300 V , 0.4-400 mA, 1-75 W . Constant voltage, current or Power with Automatic crossover Memory storage: 9 programs , 9 steps, Timer Control : 99 hr, 59 min Automatic Power up after Power failure, Safety features: No-load detection; sudden load change detection. Mini gel Specification- for 8 X 7 cm gels High throughput- Capable of running up to 4 mini gel (8 X 7 Cm) simultaneously. Should be supplied with the capability of running two gels . Flexible- Capable of running hand cast as well as precast gel. Running and casting module should be different Interchangeable module- Should be capable of using blotting module to do western blotting. Leak proof, tape free and easy assembly. Patented Flap wing for leak proof assembly. Permanently bonded spacer plates for leak proof, without agarose sealing & taping casting of gels. Casting frame with simple cam closure mechanism that gives precision alignment on any flat surface. Side by side casting stands that allow access to both gels simultaneously. Patented colored sample loading guides to prevent the skipping or repeated loading lanes. Modular design can be used do western blotting by using the blotting module only. Should able to run gels in 15-20 mins. Should come with buffer dam. Wet Transfer Apparatus: Should transfer two 10 x 7.5 cm gels simultaneously Should have high field strength for rapid 1 hr transfers Should be able to run overnight at low voltage Wire electrodes should be placed at least 4 cm apart for strong electrical fields and efficient protein transfer Colour-coded cassettes and electrodes for proper orientation of the gel during transfer Should have Ice cooling unit, completely contained within the apparatus, for absorbing heat generated during rapid transfers Volume of buffer: 450ml

Bio-safety Cabinet Cabinet must be Type B2 Should maintain minimum average inflow velocity of 100 fpm through the work area access openings. Must be able to exhaust all inflow and down flow air to the atmosphere after filtration through HEPA filter without recirculation in the cabinet or return to the laboratory room air. Material of construction should be of stainless steel. Pre-filter – 10 microns HEPA filter – 0.3 microns. Must be provided with a fluorescent lamp and UV light. Requirements for Pre-filters: Type: Flange type. Media: Synthesis, non-oven polyester fibre, washable. Casting: M.S. Powder coated. Gasket: Neoprene. Retention: 10 micron. Efficeincy: 90% Pressure drop: 6 to 8 mm

DEWINTER ‘SMART’ TRINOCULAR MICROSCOPE FOR DIGITAL CAMERA ATTACHMENT WITH SOFTWARE FOR COMPUTER CONNECTIVITY MECHANICAL BODY : Co-axial focussing control with large knob, PRE-FOCUSSING LEVER & TENSION ADJUSTMENT RING. Coarse and fine adjustment more 13mm.The slow motion has 1 Div – 0.002mm. Reverse angle quadruple nosepiece extra large revolving on ball bearings. with knurled grip for easy rotation. Double Layer ( Plate )Mechanical stage size 140mm x 135 mm & having low positioned co-axial controls on ball bearing guideways. Moving range : 75 x 50mm TRINOCULAR : Sliding Binocular head rotatable 3600 inclined at 450. Diopter adjustment ring on ocular tube on left. Third port (Trino tube) for camera attachment. SUB STAGE CONDENSER : Abbe Condenser N.A.1.25 with adjustable, Iris Diaphragm. ILLUMINATOR : 3.3W LED Illuminator ( SMPS Based ) with adjustable brightness with intergrated blue filter Inside the lamp housing. EYEPIECES : Wide field Eyepiece WF-10x (Paired) FOV 18mm OBJECTIVES COATED ACHROMAT PLAN (ANTI-FUNGUS AND ABERRATION FREE) : AP : 4x N.A. 0.10. AP 10x N.A. 0.25 . AP 40x (SL) N.A. 0.65 AP 100x (SL) Oil immersion. N.A. 1.25 STANDARD ACCESSORIES : 01. STYRO FOAM Moulded Pack. 02. Dust Cover