MANPOWER OUTSOURCE FOR THE BIOCENTRE LABS FOR LAB WORK AND CLEANING WORKS 2026-27

WASHING YARD

Loading/unloading of contaminated / used bottles for sterilization in autoclave, along with waste media disposal. (Nos.)

Over night hot water chemical soaking of baby jar bottles and caps, consolidated 4 time washing of bottles along with caps, (two time machine and two times hand) later shiftted to sterilization (Nos.)

LABORATORY (MATERIAL PREPARATION ONLY)

Pre washed bottles and caps are washed with distilled water and loaded to autoclave for sterilization, later bottle caps are oven dried after cleaning with cotton swab dipped in surface sterilizer. (Nos.)

Material for daily lab activities: (two time washing of Test tube/Conical flask/Forecep/Blade holder with the help of brush in hot water then sealed in PP cover). (Filling up of cotton/filter paper in the bottles/PP covers, sealed and arranged in the media storage room after sterilization in the autoclave. (Nos.)

Material for explant Preparation: Washing of conical flask, collection of distilled, loading/unloading for sterilization, film wrapping & arranging in the media storage room (Nos.)

Preparation of methanol solution in prescribed quantity. (Ltr)

Washing of lab coats/mask/caps/slippers, Vaccum cleaning of sterile room, weighing of chemicals/fumigants for fumigation of lab and other minor cleaning etc., (One time)

Overnight soaking of glass beads in soap solution, rinsing with methanol solution, drying/filling/autoclave sterlizing/wrapping and assembling in media storage room (Kg) once a week

Chemical/detergent washing and drying of material/utensils used during media preparation (cooker, utensil, beaker, trolley, trays (One time)

Loading/unloading of media filled bottles in autoclave for sterilization, wrapping and arranging in the media storage room (Nos.)

LABORATORY (PRODUCTION RELATED ONLY)

Stock preparation: Chemical weighing, Dilution, labeling, filtering & storing in refrigerator without getting contaminated (Skilled work) (Ltr.)

Preparation of media using stock solution, weighing of other chemicals, pH maintainance, media dispensing, capping, labeling & sterilization. (skilled work) (Ltr)

Standardization/development of protocol for invitro micro propogation of various new plants - Skilled work (per day)

Weighing of chemicals/solutions for overnight soaking treatment of explant in Antifungal & Antibacterial solution (Skilled work) (Nos.)

Prepared explant are washed/treated for 4 to 5 times and trimmed layer by layer for 2 times using (antibacterial/ antifungal /surftants/ antioxidents/bleaching agents and sterile distilled water, final washing in LAF using sterilent and inculation/labeling (skilled work) (Nos.)

Inspection and distrubution of contamination free culture bottles /media bottles for subculturing in inoculation room from media storage and growth room. (Skilled work). (Nos.)

Subculturing /wrapping/labeling /arranging of cultured bottles in growth room.(skilled work)

Inspection/Indentification/Recording/disposing of defective/offtype/contaminated culture bottles in the growth room. (Nos.) (Skilled work)

Inspection/Indentification/Recording/dispatching of fully grown ready to harden rooted plants to the washing area. (No. of bottles) (Skilled work)

2-3 times washing of rooted plants dipping antifungal solutions, grading/sorting followed by transfering them to the primary hardening chamber (No. of plants)

LABORATORY (EQUIPMENT MAINTAINCE)

Maintenance and management of Autoclave, Boiler, Millipore, Water softening unit, Generator (using log book), Hot Air Oven, Rotary shaker, Growth room UV lights/Air conditioning and other equipment (Skilled work) (per hour)

Cleaning of tissue culture laboratory and corridors (sqm)

Daily cleaning of Inoculation, R&D and other rooms floor/ chair/trolley/laminar equipments and spraying of chemicals to the walls of sterile rooms. (one time)

Every day switching on the UV lights of LAF for surface sterilization, cleaning and made ready for usage (hour/LAF)

Cleaning and maintanance work of water storage tank/sump (1 time per month)

HARNDENING UNIT

Preparation and filling the poting mixture in protrays followed by the planting rooted plants in the protrays, labeling and arranging the trays in the constructed poly tunnel in the greenhouse. (Plants)

Watering the protray plants - using rose head can (Plants)

Transplanting of primary hardened protray plants to poly bag, arranging the bags on the raised bed poly tunnels covered with polythene mulch (skilled work) (Plants)

Watering the polybags secondary hardening plants - using hose pipe (Plants)

Shiffting and arranging tissue culture plants from poly house to shade house (Nos.)

Sorting/grading/rearranging of well secondary hardened ready to plant polybag plants in trays. (Nos. of plants)

Indentification/Collection/Packing/Labeling of samples from mother stock culture/ hardening stage plants for virus indexing and genetic fidelity testing. (Nos.) Skilled work

Green House Maintenance (Side Rolling Opening and Closing / Maintenance of Foggers, Fan and Pads/ground watering. (1 time)

Technical Assistants to the Laboratory. (Post Graduate in Biotechnology) - (per day)

Collection of soil, water, leaf and organic manure samples from technical personnel.

Receiving Soil / water / leaf / organic manure samples, record, label and send it to the laboratory for analysis.

Removing and cleaning the litter, stone in the soil samples. Sieving the soil, sample and fill into the glass containers and numbering.

Drying and powdering of leaf samples, sieving , numbering and preparing the samples for analysis

Filling and numbering the water samples into the respective glasswares, drying, filling and numbering of organic manure samples into the respective glasswares.

The glasswares which are used for the various parameters were washed once with water, then rinsed with distilled water and finally prepared for different analysis.

Preparation of filter papers for analysis of potash, phosphorus, Micronutrients, Secondary Nutrients and heavy metals.

To analyse pH and EC, prepared samples are weighed and added in beaker. Measured distilled water is added in the beaker and stirred well with a glass rod. Calibrate the pH and E.C meter with buffer solution and record the readings.

Soil Samples

Water Samples

Organic Manure Samples

To analyse Phosphorous, add weighed samples into the labelled glasswares and mix with measured reagents and keep in shaker. Weigh and prepare the chemicals needed for analysis. Filter the sample after the specified time.Then record the reading on the calibrated UV spectrophotometer. Prepare the Graph of Standard Phosphorus. Prepare standards for phosphorus analysis.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyse potash, weigh the samples and add to the labelled conical flasks and then add the reagent in the shaker. Weigh and prepare chemicals needed for analysis. Filter it after the specified time and then record the reading on the calibrated flame photometer. Prepare the Potash Standards.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyse organic carbon, weigh the samples and chemicals required for analysis. Label the glassware and add the samples and mix with chemicals for analysis.

Soil Samples

Organic Manure Samples

Weigh the soil samples for nitrogen analysis and add into the kjeldahl distillation tube with required chemicals and titrate

For analysis of Nitrogen, weigh and mix the acid and add the leaf sample for digestion in a kjeldahl distillation tube on a hot plate. Add required chemicals and titrate.

For analysis of Nitrogen, in organic manure sample weigh and mix the acid for digestion on a hot plate, place it in a kjeldahl distillation tube with required chemicals for distilation and titrate

To analyse sodium in water samples, prepare the standards and record the readings in a flame photometer.

To analyse chloride in water samples, prepare chemicals, label the glasswares and titrate with required chemicals and record the readings

To analyse bicarbonate in water samples, prepare chemicals, label the glasswares and titrate with required chemicals and record the readings

To analyze micronutrients, Prepare standards, weigh the sample and put them into labelled glassware and mix with required chemicals and keep it in shaker. After speicified time remove the glassware from shaker and filter it. Analyse the sample in AAS / ICPOES.

Soil Samples

Water Samples

Leaf Samples

Organic Manure Samples

To analyze secondary nutrients, Prepare standards, weigh the sample and put them into labelled glassware and mix with required reagents and place them in the Orbiter Shaker. After speicified time remove the glassware from shaker and filter it and record the reading in UV spectrophotometer/AAS / ICPOES.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyse Boron, prepare reagent and buffer solution, weigh the sample and put them into labelled glassware mix with required chemicals and record the reading on the UV spectrophotometer / ICPOES. Prepare a standard graph for boron analysis.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyze Heavy metals, prepare standards, weigh the sample and put them into labelled glassware mix with required chemicals and placing them in shaker. After speicified time, remove the glassware from shaker and filter it. Analyse the sample in AAS / ICPOES.

Soil Samples

Water Samples

Leaf Samples

Organic Manure Samples

Soil, water, leaf and organic manure samples anlysis values are recorded and report is prepared.

Cleaning and maintenance of laboratory equipment.

Washing and cleaning of laboratory rooms and platforms.

To analyse pH and EC, samples are mixed with water. Calibrate the EC and pH meters with buffer solution and record the readings.

To analyse particular microorganisms, prepare the solution using weighing certified chemicals. For serial dilution, required test tubes, petriplates and media which are to be autoclaved is prepared.

Serial dilution is done in laminar air flow by using prepared test tubes and petriplates. Place the media in petriplates and inoculate it. seal with cling wrap.

Prepare of glass slides for staining and recording observations using microscope and prepare reports.Preference is given for B.Sc (Microbiology) to supervise and carryout the works mentioned above.

The glasswares used for the analysis is washed once in water, rinsed in clean water and finally rinsed in distilled water.

Preparation of subculture-I, weigh the certified chemicals and prepare the media for required micro organisms and autoclave. Cut the cotton and tie it to the test tubes and flasks. Inoculate the media by using prepared tubes and petriplates under laminar airflow .Seal it with cling wrap.

Preparation of subculture- II: weigh the certified chemicals and prepare the media required for micro organisms and autoclave. Using autoclaved testtubes, Petriplates and prepared media inoculate the culture in laminar air flow. To prepapre sub culture for different microorganisms, weigh the required chemicals and prepare media (Skill work) .

To prepare Starter culture- I, weigh the certified chemicals and prepare the media required for micro organisms and autoclave the media Using autoclaved testtubes, Petriplates and prepared media inoculate the culture in laminar air flow. To prepapre sub culture for different microorganisms, weigh the required chemicals and prepare media (Skill work) (For Trichoderma only)

Preparation of Starter culture- II: weigh the certified chemicals and prepare the media required for micro organisms and autoclave. Using autoclaved testtubes, Petriplates and prepared media inoculate the culture in laminar air flow. To prepapre sub culture (Broth media) for different microorganisms, weigh the required chemicals and prepare media (Skill work)

Weigh the certified chemicals to prepare mass culture media for suitable micro organisms and autoclave (For Trichoderma only).

After cooling the media , inoculate in laminar air flow. Take the suitable amount of cotton to plug the test tubes and flasks and place it in incubator for shaking.

Rinse and sterilize glasswares once in water and finally in distilled water - conical flasks, glass bottles and trays, test tubes, pittridish and pipettes.

Cleaning and maintenance of laboratory equipments.

Cleaning and fumigating the laboratary daily after analysis and culture preparation.

Preparation of subculture-I, weigh the certified chemicals and prepare the media for required micro organisms and autoclave. Cut the cotton and tie it to the test tubes and flasks. Inoculate the media by using prepared tubes and petriplates under laminar airflow .Seal it with cling wrap.

Preparation of subculture- II: weigh the certified chemicals and prepare the media required for micro organisms and autoclave. Using autoclaved testtubes, Petriplates and prepared media inoculate the culture in laminar air flow. To prepapre sub culture for different microorganisms, weigh the required chemicals and prepare media (Skill work) .

Preparation of Starter culture- II: weigh the certified chemicals and prepare the media required for micro organisms and autoclave. Using autoclaved testtubes, Petriplates and prepared media inoculate the culture in laminar air flow. To prepapre sub culture (Broth media) for different microorganisms, weigh the required chemicals and prepare media (Skill work)

After cooling the media , inoculate in laminar air flow. Take the suitable amount of cotton to plug the test tubes and flasks and place it in incubator for shaking.

Maintainance of Autoclave, Distilation unit, Laminor airflow.

Wash the glasswares using soap. Rinse the washed glasswares in distilled water. Dip the glasswares in chromic acid to streilize, finally rinse in distilled water - conical flasks, glass bottles and trays, test tubes, petridish and pipettes.

Remove the contaminated media / culture from conical flasks, glass bottles and trays, test tubes, petridish and pipettes. Sterilize them in autoclave and follow the cleaning method as per sl.no(6)

Cleaning and maintenance of laboratory equipments.

Wet mopping of platforms, working table and floor in the laboratory. Then, wet mopping disinfectant/ antibacterial chemicals/antiviral chemicals. Cleaning of Laboratory corridor. Clean and mopping of soil sieving/soil preparation room.

Fumigation of laminar airflow rooms, growth rooms and autoclave rooms before and after the production of individual biofertilizer culture.

Cleaning the three fermenters and the semi-automatic filling machine and the 600L storage tank twice (once in tap water and once in hot water) in the production unit (before media preparation)

Fumigation of fermenter rooms and packing rooms before production of individual Biofertilizers.

Transport of 03 separate media from the laboratory to the production unit and transfer them into separate 03 fermenters. Agitating during growth.

Inoculation of the culture transported from laboratory to the cooled media, specific in Fermenters,

Culture Inoculation of Laboratory Transmission and Vent Wads from the Production Unit to Laboratory and Sterilization. Sterilize manufactured bottles and transport vented vads to production plants.

500 ml bottels and vented vad

1 L bottels and vented vad

5 L bottels and vented vad

Preparation of 5% Glycerine. Collection of three seperate biofertilizers into the storage tank and addition of prepared glycerine to it.

Filling the bottles with Biofertilizers through semi automatic filling machine.

500 ml bottels

1 L bottels

5 L bottels

Filling the prepared biofertilizers to half / one / five liters of autoclaved bottles and then place the vented vad and lid to the bottels and arrange the bottels in a proper manner. (Frequent cleaning in case of leakage)

Print the date of produciton, date of expiry, bacth number on lables (which contain information about biofertilizer). Stick the printed labels to the bottles.

The labled bottles are transported from production site and assembled in a cooler place.

Transportion of prepared product from production site to sales counter (only is case of necessity)

The bottles to be transported each district/taluk must be placed in a carton box and sealed. Assemble the carton boxes particular to the district/taluk. (Bottles) (Sending only for delivery under schemes)

Filling the boiler with water and mantainance of presurised equipments like fermeters and air compressors.

General cleaning and fumigation of fermenter room, mixing and packing area and godown.Cleaning of mixing machine, packing machine, weighing scale, etc..

Cleaning, rinsing and preparation of ingredients for bio inputs production. (Trichoderma only)

Store the prepared media and then sterilize in a fermentor. After cooling, inoculate in the autoclaved buckets / trays /drums. (Trichoderma only)

Cleaning the fermentor in the production unit. Sterilize the media of biofertilizer/ bio control agent prepared from the laboratory in fermetor. Inoculate the culture upon cooling.

After Pasteurization of Lignite / Talc Powder, draw required amount of culture from fermentor and mix it. Again mix with Carboxyl Methanol Cellulose. Beat the lumps and transfer the fine powder to packing area.

Mixed biofertilizers / bio control agents weighed, packed and sealed into 1 kg Milky Pouch (8X9). These milky pouches are further packed, sealed, labelled into printed covers with production details and stacked.

Filling and sealing of bioinputs into 50 kg each bag for transportation to district / taluk and stacked according to categories.

Cleaning and fumigating all the equipments (mixing mission, packing mission, weighing scale, etc.) and maintaining hygienic environment in the rooms of fermentor, mixing area, packing area and godown.

Storing agricultural wastes from different sources, separating plastic and other materials, preparing them properly and filling it to pit. (10 'X 4' X 21/2 ') (5 trough)

Watering the vermicompost pit twice a week.

Mix the cowdung with water twice (after 15 to 12 days) and spray on agriculture waste in the pit.

After 15 to 20 days, mix with half decomposed wastes in the existing pit and transfer it to other pit.

Decomposed mixture is added with earthworms. After 20 days, pour on the ground and seperate the earthworms present at bottom. These earthworms are added to the pits which is ready for new batch.

Separate the cocoons, juveniles, hatchlings and the food left by earthworms are sieved.

Weigh into 5kg / 10kg / 20kg / 50 kg, that are sewed and stacked in a cool room. (New addition)

To prepare the VAM culture in pots (12" pots), strelize it and fill it with the mixture of sand, soil and manure in 1:1:1 ratio.

Prepared soil mixture is added with VAM Mother Culture. Sowing seeds of millets or sorghum to VAM Mother Culture treated pots. (New addition)

Watering the of VAM treated pots, lightly during the entire crop period.

Weeding twice a week in VAM cultured pots.

Allow to grow Ragi/Sorghum plant for 75 days (2 ½ month). Cut the plants from base mix with soil along with roots. The prepared VAM culture is used to prepare the VAM biofertilizer.

The beds used for VAM preparation, are cleaned and spread with black colour polythene sheet. Prepare the media of bed with mixture of soil, sand, vermicullite and farm yard manure. Prepared beds (25' X 4' X 1' measurement) are covered with black colour polythene sheet for a week.

After 1 week, prepared VAM culture is mixed with beds with specified quantity. Ragi/Sorghum/any other seeds are sown into the beds.

Water the crop for whole growing period in pots. Irrigate the sown beds for 8 weeks (56 days) for whole crop life cycle.

Weed the bed 4 times (for whole crop life cycle).

After 8 to 10 weeks (56 to 70days), harvest the crop and incorporate into the beds and leave it for 7 days. Later VAM roots are powdered and shade dried, packed into 5/10/20 Kg bags and sealed.

To prepare biomix, the vermicompost is sieved and stored. The stored vermicompost manure is added with specified quantity of Arka Microbial Consortium Biofertilizers.

Mixed biomix is weighed and filled into 5kg / 10kg poly sack bags. Seal the weighed bags and stack them in a room with cool temperature.

Pesticide residue analysis :

Clean the samples and rinse with water. Take the specified amount of the samples and chop into small pieces. The chopped samples are grinded in a grinder with distilled water, then transferred to a jar bottle, labelled and stored.

Preparation of muffle furnace for activation of certain chemicals. Before analysing in LCMS/MS instrument, prepare the mobile phase (mixing the specified chemicals) and sonificate in the sonicator instrument.

Before performing the analysis in the GCMS/MS, Baseline correction is performed by stabilizing the instrument. After switching on the instrument, generate the vacuum and bring the instrument to a stabilization stage.

Weigh the Certified Reference Materials precisely for preparation of primary standards. Then transfer into volumetric flasks to prepare the working standards as per SoP and store it in the Deep Freezer.

Prepare the instrument by calibrating for analysis. Weigh the stored sample accurately. Add required solvent and distilled water to the weighed sample. To that sample add required chemicals and mix well.

Clean the homogenizer in distilled water and homogenize the blended sample. Centrifuge the homogenized sample and transfer the supernatant into vortex and re-centrifugate and filter.

For analysis of pesticide residues in LCMS, reconstitute the solvent in nitrogen concentrator and tranfer the sample into 1 ml vials

Preparing the instrument for analysis (sample sequence table, labelling, details, setting of parameters, etc) and feed the sample for analysis. The final stage of the analysis, data processing, quantification, qualification and interpretating the result to generate documents and reports.

Washing the column of equipment after each sample analysis. Cleaning and maintenance of laboratory equipment.

Washing the glass equipment once in water after that rinsing it in distilled water - test tubes, beaker, tray, jar bottle,

Platforms and floor cleaning in the laboratory.

Technical Assistant for laboratory (Post graduate in Biotechnology/Microbiology)

Pure Culture preparation

Contaminated/used test tubes/petriplates are taken out of the laboratory and cleaned with brush, hotwater, distilled water. Cleaned test tubes/petriplates are wrapped in paper and placed in PP cover, sterilized and oven dried (no)

cotton plugs are prepared : by cutting the cotton which is sutable to test tube mouth(no)

Measurement of chemicals required for media preparation. Preparation of media solution. Prepared media is filled to conical flask/test tube and sterilized in autoclave (skilled work)(no).

Sterilized media is taken from autoclave and required amount of media is poured in to petriplates. Testubes are kept in slants.(no).

Inoculating the media with pure culture / sub culture (no) (skilled work)

Steps involved in commercial spawn preparation

Required bottles for spawn prepation are washed with brush, hot water and then sterilized in autoclave. (no)

Cotton plug preparation which is requried for commercial spawn and p v c ring preparation . (no)

Cleaning of white jowar seeds, boiling , mixing with chalk powder and filling in pp cover/ sterilized bottles, plugging with cotton, loading in autoclave for sterilization, after sterilization bottles/PP cover are kept in inoculation chamber under laminar air flow for innoculation. (1kg packet/bottle)

Inoculation of Mother spawn bottles under laminar air flow and incubation in BOD Incubator (no). (skilled work)

Inoculation of generation-1/ generation- 2 spawn bottles and placing for incubation in BOD Incubator (no). (skilled work)

Inoculation of Commercial spawn packets and incubation in BOD Incubator (no).

Production of different varieties of mushroom.oyster and milky (1to3)

Dry paddy straw are cut into 2.5 to 3 inches, soaked in cold water and shade dried.(kg)

Optimally dried paddy straw are filled in PP cover, mouth of PP cover are tied using PVC ring and and stuffed with non absorbent cotton and kept in autoclave for sterilization.(no)

After sterilization bags are taken out of autoclave and allowed for cooling of bags, bags are inoculated with spawn and placed in spawn running room for incubation.(no)

Oyster mushroom : after spawn run the bags are shifted to cropping room for cropping (no)

milky mushroom : preparation of casing mixture. (kg)

milky mushroom: after completion of spawn running, casing layer is applied on bags and bags are kept in cropping room

shiitake mushroom

shiitake mushroom:- requried amount of saw dust, wood chips, rice/wheat bran and chalk powder are weighed and mixed with water. Prepared substrate is sterilized in autoclave, sterilized substrate is inoculated under laminar air flow and placed in spawn run room for incubation. (kg)

cold water treatment is given after spawn running for crop initiation (once) (no)

maintenance of bags in cropping room .(spraying of water/relative humidity/temperature/ etc ) (no)

harvesting of cultivated mushroom and packing. (kg)

white button mushroom :-

preparation of compost and pasteurizing the prepared compost. (kg)

preparation of 5 kg pasteurised compost bags and inoculating them with spawn and arranging the bags in incubation room for mycelium development (nos)

Application of casing material and shifting to the cropping room (no)

Maintenance of bags in cropping room .(spraying of water/relative humidity/temperature/ etc ) (no)

Harvesting of cultivated mushroom and packing. (kg)

Preparation of casing material and pasteurisation (kg)

Miscallaneous activities

Maintenance of cleanliness in and around the laboratory. Periodic washing and cleaning of lab apparel, foot mat and other materials. Removal of weed growth around the lab and other related activities

Regular maintenance of Grain cleaner, Grain Boiler, Mixing cum filler Machine, Autoclaves, Hot Air Oven and other equipment

Spraying/fumigation in the lab with disinfectants (one)

Drying and dehydration of freshly harvested mushrooms (kg)

Inspection and Examination of inoculated Test Tubes/Petri plates/bottles of various generations/commercial spawn for contamination (skilled work)

Washing of contaminated Test Tubes/Petri plates/bottles, sterilising them and drying in hot air oven

Transferring of contaminated Spawn packets/RTF bag and spent mushroom substrate to composting pits

Land preparation, ploughing, leveling of land for new seed bed preparation: Medium soil

Clearing of unwanted vegetation by cutting and uprooting shrubs, small plants etc and cleaning of seed bed area. Chopped material to be placed along the borders of the seed bed

Land preparation, ploughing, leveling of land for new seed bed preparation: Hard soil

Preparing and filling of 4" X 6" Poly bags with red soil, sand, fim with required proportion

Preparing and filling of 5" X 8" Poly bags with red soil, sand, fim with required proportion

Preparing and filling of 6" X 9" Poly bags with red soil, sand, fim with required proportion

Sowing of seeds/seedlings in poly bags

Watering of poly bags twice daily- 4" X 6" Poly bags

Watering of poly bags twice daily- 5" X 8" Poly bags

Watering of poly bags twice daily- 6" X 9" Poly bags

Weeding of poly bags (All sizes)

Lifting and rearranging of poly bags containing seedlings - 4" X 6" Poly bags

Lifting and rearranging of poly bags containing seedlings - 5" X 8" Poly bags

Lifting and rearranging of poly bags containing seedlings - 6" X 9" Poly bags

Preparing Spray mixture and spraying of plant preotection chemicals to poly bags containg seedlings-4" X 6" Poly bags

Preparing Spray mixture and spraying of plant preotection chemicals to poly bags containg seedlings-5" X 8" Poly bags

Preparing Spray mixture and spraying of plant preotection chemicals to poly bags containg seedlings-6" X 9" Poly bags

Preparation of land for seed bed preparation for coconut seed sowing 8 X 1.25 mtr(100 seed nuts per seed bed), preparing trenches for drainage (December to March 4 months) not excluding seed and plant protection cost.

Watering, weeding, plant protection etc., for coconut seed bed (April to March)

Removal of parasite plants from sapota/Mango plant branches

Manuring/sowing/roughing/inter cultivation and weeding twice/harvesting/picking seeds (Vegetable seed production)

Scion preparation for Mango/Jack/Jamun propogation

Scion preparation for Amla/ Guava propogation

Scion preparation for Custard apple propogation

Grafting of fruit plants

8 X 1.25 mtr seed bed preparation

Maintenance of fruit plant seedings poly house/shade net

8 X 1.25 mtr seed bed preparation and sowing of Mango stones

Preparation seed bed for production of rooted seedlings Guava/Amla/custard apple/Jamun etc., and maintainance

Cleaning of land- medium forest

Digging of pits and lifting of soil from pits -medium soil 3' x 3' x 3'

Digging of pits and lifting of soil from pits -Hard soil 3' x 3' x 3'

Preparation of soil mixture and filling it 90% of a pit. Hard soil 3' x 3' x 3'

Lifting of plants to the pit and planting and staking

Weeding and basin preparation around plants -0.6 meter radius

Weeding and basin preparation around plants -1.2 meter radius

Weeding and basin preparation around plants -1.8 meter radius

Weeding and basin preparation around plants -2.40 meter radius

Weeding and basin preparation around plants -3.0 meter radius

Applying organic fertilizer to plants -0.6 meter radius

Applying organic fertilizer to plants -1.2 meter radius

Applying organic fertilizer to plants -1.8 meter radius

Applying organic fertilizer to plants -2.40 meter radius

Applying organic fertilizer to plants -3.0 meter radius

Digging, weeding and cleaning in farm (1 X 1 X 0.45 Mtr= 0.45 cubic Meter)

Ploughing of land (In Tractor Cultivator)

Ploughing of land (with M B Plougher)

Ploughing of land (with Disc)

0.6 X 1 X 1 mtr Basin preparation

0.6 X 0.6 X 1 mtr Basin preparation

Piting and lifting of soil from pit with JCB

Preparation of soil mixture and filling of pots with the mixture and scribling of land and assembling the pots- 9" pots

Preparation of soil mixture and filling of pots with the mixture and scribling of land and assembling the pots- 12" pots

Preparation of soil mixture and filling of pots with the mixture and scribling of land and assembling the pots- 14" pots

Preparation of soil mixture and filling of tubs with the mixture and scribling of land and assembling the tubs- 18" tubs

Preparation of soil mixture and filling of tubs with the mixture and scribling of land and assembling the tubs- 24" tubs

Preparation of soil mixture and filling of seed pan with the mixture and scribling of land and assembling the seed pan- 12" seed pan

Weeding of pots and tubs, loosning of soil and manuring- 9" pots

Weeding of pots and tubs, loosning of soil and manuring- 12" pots

Weeding of pots and tubs, loosning of soil and manuring- 14" pots

Weeding of pots and tubs, loosning of soil and manuring- 18" tubs

Weeding of pots and tubs, loosning of soil and manuring- 24" tubs

Ploughing of land to extent of 1 1/2 ' (0.45 mtr) depth and refilling of the pit with 50% of the top soil and remaining 50% with red soil, sand and fym (1:2:3) for seed sowing, bulb sowing(including seed/bulb/seedling cost)- Medium soil

Ploughing of land to extent of 1 1/2 ' (0.45 mtr) depth and refilling of the pit with 50% of the top soil and remaining 50% with red soil, sand and fym (1:2:3) for seed sowing, bulb sowing(including seed/bulb/seedling cost)- Hard soil

Weeding of pots and tubs, loosning of soil and manuring- 12" Seed pan

Cutting preparation - Soft/hard wood cuttings (without spines cuttings)

Cutting preparation - Soft/hard wood cuttings (with spines cuttings)

Filling of seed pans with soil mixture and sowing of seeds

Indoor plants cuttings(Difenbechia, aglonema, Peperomia, etc.,)

Watering of Plants with Hose pipes

Watering of Plants Manually

Growing of rose stock plants in poly bags

Propagation of Guava, Fig, pomegranate, roseapple by layering

Cleaning of sump and water tank

Maintainence of greenhouse (Swicthing on and off fan and pad, maintaining shade net, watering inside the greenhouse)

Loading of poly bags with plants to the lorry -4" X 6"

Loading of poly bags with plants to the lorry -5" X 8"

Loading of poly bags with plants to the lorry -6" X 9"

preparation of seedling production media and sowing of ornamental/vegetable seeds for propagation of ornamental and vegetable seedlings- 11 protrays

Management of ornamental and vegetable seedlings (1.5 months) 11 protrays

Digging 0.75 m X 0.75 m X 0.75 m and refilling of the pit with 50% of the top soil and remaining 50% with sand ( 0.035 cubic Meter),fym(0.070 cubic meter) and red soil (0.105 Cubic Meter) in 1:2:3 proportion and planting of well grown plant. (excluding plant cost) - Medium soil

Digging 0.75 m X 0.75 m X 0.75 m and refilling of the pit with 50% of the top soil and remaining 50% with sand ( 0.035 cubic Meter),fym(0.070 cubic meter) and red soil (0.105 Cubic Meter) in 1:2:3 proportion and planting of well grown plant. (excluding plant cost) - Hard soil

Digging 0.45 m X 0.45 m X 0.45 m and refilling of the pit with 50% of the top soil and remaining 50% with sand ( 0.007 cubic Meter),fym(0.014 cubic meter) and red soil (0.021 Cubic Meter) in 1:2:3 proportion and planting of well grown plant. (excluding plant cost) - Medium soil

Digging 0.45 m X 0.45 m X 0.45 m and refilling of the pit with 50% of the top soil and remaining 50% with sand ( 0.007 cubic Meter),fym(0.014 cubic meter) and red soil (0.021 Cubic Meter) in 1:2:3 proportion and planting of well grown plant. (excluding plant cost) - Hard soil

Cutting of dense grown un wanted plants and removal of bush along with roots

Watering of fruit plants with pitcher `

Identifying trees and removal/cutting of un wanted branches and pasting with bordeaux paste

cutting of green manure plants and mulching them or collection of leaf litter and mulching the basin of plants

Collection of fallen coconut fronds from the coconut plantation (chopping of fronds and using them as mulch)

Thinning out of branches of mango trees

setting up of fire safety ring excluding canopy 1 metre radius

fire safety methods- Cutting/removing, collection and burning of unwanted grasses, shrubs, weeds etc up to a width of 3m

Digging of catch pit (2x2x2) feet - 2 pits per tree (Rs. 30 per pit)

Kannada Papers

English papers

Description of works

Collection of soil, water, leaf and organic manure samples from technical personnel.

Receiving Soil / water / leaf / organic manure samples, record, label and send it to the laboratory for analysis.

Removing and cleaning the litter, stone in the soil samples. Sieving the soil, sample and fill into the glass containers and numbering.

Drying and powdering of leaf samples, sieving , numbering and preparing the samples for analysis

Filling and numbering the water samples into the respective glasswares, drying, filling and numbering of organic manure samples into the respective glasswares.

The glasswares which are used for the various parameters were washed once with water, then rinsed with distilled water and finally prepared for different analysis.

Preparation of filter papers for analysis of potash, phosphorus, Micronutrients, Secondary Nutrients and heavy metals.

To analyse pH and EC, prepared samples are weighed and added in beaker. Measured distilled water is added in the beaker and stirred well with a glass rod. Calibrate the pH and E.C meter with buffer solution and record the readings.

Soil Samples

Water Samples

Organic Manure Samples

To analyse Phosphorous, add weighed samples into the labelled glasswares and mix with measured reagents and keep in shaker. Weigh and prepare the chemicals needed for analysis. Filter the sample after the specified time.Then record the reading on the calibrated UV spectrophotometer. Prepare the Graph of Standard Phosphorus. Prepare standards for phosphorus analysis.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyse potash, weigh the samples and add to the labelled conical flasks and then add the reagent in the shaker. Weigh and prepare chemicals needed for analysis. Filter it after the specified time and then record the reading on the calibrated flame photometer. Prepare the Potash Standards.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyse organic carbon, weigh the samples and chemicals required for analysis. Label the glassware and add the samples and mix with chemicals for analysis.

Soil Samples

Organic Manure Samples

Weigh the soil samples for nitrogen analysis and add into the kjeldahl distillation tube with required chemicals and titrate

For analysis of Nitrogen, weigh and mix the acid and add the leaf sample for digestion in a kjeldahl distillation tube on a hot plate. Add required chemicals and titrate.

For analysis of Nitrogen, in organic manure sample weigh and mix the acid for digestion on a hot plate, place it in a kjeldahl distillation tube with required chemicals for distilation and titrate

To analyse sodium in water samples, prepare the standards and record the readings in a flame photometer.

To analyse chloride in water samples, prepare chemicals, label the glasswares and titrate with required chemicals and record the readings

To analyse bicarbonate in water samples, prepare chemicals, label the glasswares and titrate with required chemicals and record the readings

To analyze micronutrients, Prepare standards, weigh the sample and put them into labelled glassware and mix with required chemicals and keep it in shaker. After speicified time remove the glassware from shaker and filter it. Analyse the sample in AAS / ICPOES.

Soil Samples

Water Samples

Leaf Samples

Organic Manure Samples

To analyze secondary nutrients, Prepare standards, weigh the sample and put them into labelled glassware and mix with required reagents and place them in the Orbiter Shaker. After speicified time remove the glassware from shaker and filter it and record the reading in UV spectrophotometer/AAS / ICPOES.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyse Boron, prepare reagent and buffer solution, weigh the sample and put them into labelled glassware mix with required chemicals and record the reading on the UV spectrophotometer / ICPOES. Prepare a standard graph for boron analysis.

Soil Samples

Leaf Samples

Organic Manure Samples

To analyze Heavy metals, prepare standards, weigh the sample and put them into labelled glassware mix with required chemicals and placing them in shaker. After speicified time, remove the glassware from shaker and filter it. Analyse the sample in AAS / ICPOES.

Soil Samples

Water Samples

Leaf Samples

Organic Manure Samples

Soil, water, leaf and organic manure samples anlysis values are recorded and report is prepared.

Cleaning and maintenance of laboratory equipment.

Washing and cleaning of laboratory rooms and platforms.

Genaral and Miscallaneous activities

Maintenance of cleanliness in and around the laboratory. Periodic washing and cleaning of lab apparel, foot mat and other materials. Removal of weed growth around the lab and other related activities

Regular maintenance of Grain cleaner, Grain Boiler, Mixing cum filler Machine, Autoclaves, Hot Air Oven and other equipment

Clearing of unwanted vegetation by cutting and uprooting shrubs, small plants etc and cleaning of seed bed area. Chopped material to be placed along the borders of the seed bed

Applying organic fertilizer to plants -3.0 meter radius

Digging, weeding and cleaning in farm (1 X 1 X 0.45 Mtr= 0.45 cubic Meter)

Ploughing of land (In Tractor Cultivator)

Ploughing of land (with M B Plougher)

Ploughing of land (with Disc)

Cleaning of sump and water tank

Maintainence of greenhouse (Swicthing on and off fan and pad, maintaining shade net, watering inside the greenhouse)

Digging 0.45 m X 0.45 m X 0.45 m and refilling of the pit with 50% of the top soil and remaining 50% with sand ( 0.007 cubic Meter),fym(0.014 cubic meter) and red soil (0.021 Cubic Meter) in 1:2:3 proportion and planting of well grown plant. (excluding plant cost) - Hard soil

Cutting of dense grown un wanted plants and removal of bush along with roots

Indoor Cleaning of Laboratory room and corridor cleaning work (1900 sqm per day) (1 Sqm = 0.43)

Outdoor Cleaning: Cleaning and Removing Dense Shrubs and cut-thin shrubs (including 0.25 m thick) and shrubs (1.5 m high 50 m away). (1500 sq. 6.75 x 2 times per month) (1 Sqm = 6.75)

Removal of unwanted trees, weeds, climbers etc Cleaning the areas with JCB

Rinse and sterilize glasswares once in water and finally in distilled water - conical flasks, glass bottles and trays, test tubes, pittridish and pipettes.

Cleaning and maintenance of laboratory equipments.

Cleaning and fumigating the laboratary daily after analysis and culture preparation.

Cleaning, rinsing and preparation of ingredients for bio inputs production. (Trichoderma ,metarhizium only)

Store the prepared media and then sterilize in a fermentor. After cooling, inoculate in the autoclaved buckets / trays /drums. (Trichoderma ,metarhizium only)

After Pasteurization of Lignite / Talc Powder, draw required amount of culture from fermentor and mix it. Again mix with Carboxyl Methanol Cellulose. Beat the lumps and transfer the fine powder to packing area.

Mixed biofertilizers / bio control agents weighed, packed and sealed into 1 kg Milky Pouch (8X9). These milky pouches are further packed, sealed, labelled into printed covers with production details and stacked.

Filling and sealing of bioinputs into 50 kg each bag for transportation to district / taluk and stacked according to categories.

Cleaning and fumigating all the equipments (mixing mission, packing mission, weighing scale, etc.) and maintaining hygienic environment in the rooms of fermentor, mixing area, packing area and godown.

Rinse and sterilize glasswares once in water and finally in distilled water - conical flasks, glass bottles and trays, test tubes, pittridish and pipettes.

Cleaning and maintenance of laboratory equipments.

Cleaning and fumigating the laboratary daily after analysis and culture preparation.

Cleaning the fermentor in the production unit. Sterilize the media of biofertilizer/ bio control agent prepared from the laboratory in fermetor. Inoculate the culture upon cooling.(Pseudomonas)

After Pasteurization of Lignite / Talc Powder, draw required amount of culture from fermentor and mix it. Again mix with Carboxyl Methanol Cellulose. Beat the lumps and transfer the fine powder to packing area.

Mixed biofertilizers / bio control agents weighed, packed and sealed into 1 kg Milky Pouch (8X9). These milky pouches are further packed, sealed, labelled into printed covers with production details and stacked.

Filling and sealing of bioinputs into 50 kg each bag for transportation to district / taluk and stacked according to categories.

Cleaning and fumigating all the equipments (mixing mission, packing mission, weighing scale, etc.) and maintaining hygienic environment in the rooms of fermentor, mixing area, packing area and godown.

Wash the glasswares using soap. Rinse the washed glasswares in distilled water. Dip the glasswares in chromic acid to streilize, finally rinse in distilled water - conical flasks, glass bottles and trays, test tubes, petridish and pipettes.

Remove the contaminated media / culture from conical flasks, glass bottles and trays, test tubes, petridish and pipettes. Sterilize them in autoclave and follow the cleaning method as per sl.no(6)

Cleaning and maintenance of laboratory equipments.

Wet mopping of platforms, working table and floor in the laboratory. Then, wet mopping disinfectant/ antibacterial chemicals/antiviral chemicals. Cleaning of Laboratory corridor. Clean and mopping of soil sieving/soil preparation room.

Fumigation of laminar airflow rooms, growth rooms and autoclave rooms before and after the production of individual biofertilizer culture.

Cleaning the three fermenters and the semi-automatic filling machine and the 600L storage tank twice (once in tap water and once in hot water) in the production unit (before media preparation)

Fumigation of fermenter rooms and packing rooms before production of individual Biofertilizers.

Transport of 03 separate media from the laboratory to the production unit and transfer them into separate 03 fermenters. Agitating during growth.

Inoculation of the culture transported from laboratory to the cooled media, specific in Fermenters,

Bringing of Bottles and Vent Wads from the Production Unit to Laboratory and Sterilize. Aftre Sterilization shifting of Sterilized Bottles and Vent Wads to the Production unit.

500 ml bottels and vented vad

1 L bottels and vented vad

5 L bottels and vented vad

Preparation of 5% Glycerine. Collection of three seperate biofertilizers into the storage tank and addition of prepared glycerine to it.

Filling the bottles with Biofertilizers through semi automatic filling machine.

500 ml bottels

1 L bottels

5 L bottels

Filling the prepared biofertilizers to half / one / five liters of autoclaved bottles and then place the vented vad and lid to the bottels and arrange the bottels in a proper manner. (Frequent cleaning in case of leakage)

Print the date of produciton, date of expiry, bacth number on lables (which contain information about biofertilizer). Stick the printed labels to the bottles.

The labled bottles are transported from production site and assembled in a cooler place.

Transportion of prepared product from production site to sales counter (only is case of necessity)

The bottles to be transported each district/taluk must be placed in a carton box and sealed. Assemble the carton boxes particular to the district/taluk. (Bottles) (Sending only for delivery under schemes)

Filling the boiler with water and mantainance of presurised equipments like fermeters and air compressors.

General cleaning and fumigation of fermenter room, mixing and packing area and godown.Cleaning of mixing machine, packing machine, weighing scale, etc..

Wash the glasswares using soap. Rinse the washed glasswares in distilled water. Dip the glasswares in chromic acid to streilize, finally rinse in distilled water - conical flasks, glass bottles and trays, test tubes, petridish and pipettes.

Remove the contaminated media / culture from conical flasks, glass bottles and trays, test tubes, petridish and pipettes. Sterilize them in autoclave and follow the cleaning method as per sl.no(6)

Cleaning and maintenance of laboratory equipments.

Wet mopping of platforms, working table and floor in the laboratory. Then, wet mopping disinfectant/ antibacterial chemicals/antiviral chemicals. Cleaning of Laboratory corridor. Clean and mopping of soil sieving/soil preparation room.

Fumigation of laminar airflow rooms, growth rooms and autoclave rooms before and after the production of individual biofertilizer culture.

Cleaning the three fermenters and the semi-automatic filling machine and the 600L storage tank twice (once in tap water and once in hot water) in the production unit (before media preparation)

Fumigation of fermenter rooms and packing rooms before production of individual Biofertilizers.

Transport of 03 separate media from the laboratory to the production unit and transfer them into separate 03 fermenters. Agitating during growth.

Inoculation of the culture transported from laboratory to the cooled media, specific in Fermenters,

After Pasteurization of Lignite / Talc Powder, draw required amount of culture from fermentor and mix it. Again mix with Carboxyl Methanol Cellulose. Beat the lumps and transfer the fine powder to packing area.

Mixed biofertilizers / bio control agents weighed, packed and sealed into 1 kg Milky Pouch (8X9). These milky pouches are further packed, sealed, labelled into printed covers with production details and stacked.

Filling and sealing of bioinputs into 50 kg each bag for transportation to district / taluk and stacked according to categories.

Cleaning and fumigating all the equipments (mixing mission, packing mission, weighing scale, etc.) and maintaining hygienic environment in the rooms of fermentor, mixing area, packing area and godown.

Wash the glasswares using soap. Rinse the washed glasswares in distilled water. Dip the glasswares in chromic acid to streilize, finally rinse in distilled water - conical flasks, glass bottles and trays, test tubes, petridish and pipettes.

Remove the contaminated media / culture from conical flasks, glass bottles and trays, test tubes, petridish and pipettes. Sterilize them in autoclave and follow the cleaning method as per sl.no(6)

Cleaning and maintenance of laboratory equipments.

Wet mopping of platforms, working table and floor in the laboratory. Then, wet mopping disinfectant/ antibacterial chemicals/antiviral chemicals. Cleaning of Laboratory corridor. Clean and mopping of soil sieving/soil preparation room.

Fumigation of laminar airflow rooms, growth rooms and autoclave rooms before and after the production of individual biofertilizer culture.

Fumigation of fermenter rooms and packing rooms before production of individual Biofertilizers.

Transport of 03 separate media from the laboratory to the production unit and transfer them into separate 03 fermenters. Agitating during growth.

Inoculation of the culture transported from laboratory to the cooled media, specific in Fermenters,

After Pasteurization of Lignite / Talc Powder, draw required amount of culture from fermentor and mix it. Again mix with Carboxyl Methanol Cellulose. Beat the lumps and transfer the fine powder to packing area.

Mixed biofertilizers / bio control agents weighed, packed and sealed into 1 kg Milky Pouch (8X9). These milky pouches are further packed, sealed, labelled into printed covers with production details and stacked.

Filling and sealing of bioinputs into 50 kg each bag for transportation to district / taluk and stacked according to categories.

Cleaning and fumigating all the equipments (mixing mission, packing mission, weighing scale, etc.) and maintaining hygienic environment in the rooms of fermentor, mixing area, packing area and godown.

Storing agricultural wastes from different sources, separating plastic and other materials, preparing them properly and filling it to pit. (10 'X 4' X 21/2 ') (5 trough)

Watering the vermicompost pit twice a week.

Mix the cowdung with water twice (after 15 to 12 days) and spray on agriculture waste in the pit.

After 15 to 20 days, mix with half decomposed wastes in the existing pit and transfer it to other pit.

Decomposed mixture is added with earthworms. After 20 days, pour on the ground and seperate the earthworms present at bottom. These earthworms are added to the pits which is ready for new batch.

Separate the cocoons, juveniles, hatchlings and the food left by earthworms are sieved.

Weigh into 5kg / 10kg / 20kg / 50 kg, that are sewed and stacked in a cool room. (New addition)

To prepare biomix, the vermicompost is sieved and stored. The stored vermicompost manure is added with specified quantity of Arka Microbial Consortium Biofertilizers.

Mixed biomix is weighed and filled into 5kg / 10kg poly sack bags. Seal the weighed bags and stack them in a room with cool temperature.

WASHING YARD

Loading/unloading of contaminated / used bottles for sterilization in autoclave, along with waste media disposal. (Nos.)

Over night hot water chemical soaking of baby jar bottles and caps, consolidated 4 time washing of bottles along with caps, (two time machine and two times hand) later shiftted to sterilization (Nos.)

LABORATORY (MATERIAL PREPARATION ONLY)

Pre washed bottles and caps are washed with distilled water and loaded to autoclave for sterilization, later bottle caps are oven dried after cleaning with cotton swab dipped in surface sterilizer. (Nos.)

Material for daily lab activities: (two time washing of Test tube/Conical flask/Forecep/Blade holder with the help of brush in hot water then sealed in PP cover). (Filling up of cotton/filter paper in the bottles/PP covers, sealed and arranged in the media storage room after sterilization in the autoclave. (Nos.)

Material for explant Preparation: Washing of conical flask, collection of distilled, loading/unloading for sterilization, film wrapping & arranging in the media storage room (Nos.)

Preparation of methanol solution in prescribed quantity. (Ltr)

Bioload test, Vaccum cleaning of sterile room, weighing of chemicals/fumigants for fumigation of lab and Washing of lab coats/mask/caps/slippers, and other minor cleaning etc., (One time)

Overnight soaking of glass beads in soap solution, rinsing with methanol solution, drying/filling/autoclave sterlizing/wrapping and assembling in media storage room (Kg) once a week

Chemical/detergent washing and drying of material/utensils used during media preparation (cooker, utensil, beaker, trolley, trays (One time)

LABORATORY (PRODUCTION RELATED ONLY)

Stock preparation: Chemical weighing, Dilution, labeling, filtering & storing in refrigerator without getting contaminated (Skilled work) (Ltr.)

Media prepration: Preparation of media using stock solution, weighing of other chemicals, pH maintainance, media dispensing, capping, labeling & (skilled work) (Ltr)

Loading/unloading of media filled bottles in autoclave for sterilization, wrapping and arranging in the media storage room (Nos.)

Weighing of chemicals/solutions for overnight soaking treatment of explant in Antifungal & Antibacterial solution (Skilled work) (Nos.)

Prepared explant are washed/treated for 4 to 5 times and trimmed layer by layer for 2 times using (antibacterial/ antifungal /surftants/ antioxidents/bleaching agents and sterile distilled water, final washing in LAF using sterilent and inculation/labeling (skilled work) (Nos.)

Inspection and distrubution of contamination free culture bottles /media bottles for subculturing in inoculation room from media storage and growth room. (Skilled work). (Nos.)

Subculturing /wrapping/labeling /arranging of cultured bottles in growth room.(skilled work)

Inspection/Indentification/Recording/disposing of defective/offtype/contaminated culture bottles in the growth room. (Nos.) (Skilled work)

Inspection/Indentification/Recording/dispatching of fully grown ready to harden rooted plants to the washing area. (No. of bottles) (Skilled work)

2-3 times washing of rooted plants dipping antifungal solutions, grading/sorting followed by transfering them to the primary hardening chamber (No. of plants)

LABORATORY (EQUIPMENT MAINTAINCE)

Maintenance and management of Autoclave, Boiler, Millipore, Water softening unit, Generator (using log book), Hot Air Oven, Rotary shaker, Growth room UV lights/Air conditioning and other equipment (Skilled work) (per hour)

Cleaning of tissue culture laboratory and corridors (sqm)

Daily cleaning of Inoculation, R&D and other rooms floor/ chair/trolley/laminar equipments and spraying of chemicals to the walls of sterile rooms. (one time)

Every day switching on the UV lights of LAF for surface sterilization, cleaning and made ready for usage (hour/LAF)

Cleaning and maintanance work of water storage tank/sump (1 time per month)

HARNDENING UNIT

Washing and grading of rooted plants (No of plants)

Preparation and filling the poting mixture in protrays followed by the planting rooted plants in the protrays, labeling and arranging the trays in the constructed poly tunnel in the greenhouse. (Plants)

Watering the protray plants - using rose head can (Plants)

Green House Maintenance (Side Rolling Opening and Closing / Maintenance of Foggers, Fan and Pads/ground watering. (1 time)

Polybag media preparation and filling 4*6 nos

Transplanting of primary hardened protray plants to poly bag, arranging the bags on the raised bed poly tunnels covered with polythene mulch (skilled work) (Plants)

Watering the polybags secondary hardening plants - using hose pipe (Plants)

Spraying of plant protectants nutrients to polybags using hosepipe (Nos of plants)

Weeding in polybags

Shiffting and arranging tissue culture plants from poly house to shade house (Nos.)

Sorting/grading/rearranging of well secondary hardened ready to plant polybag plants in trays. (Nos. of plants)

Loading of hardenned plants for sale (No of plants)

Indentification/Collection/Packing/Labeling of samples from mother stock culture/ hardening stage plants for virus indexing and genetic fidelity testing. (Nos.) Skilled work

Technical Assistants to the Laboratory. (Post Graduate in Biotechnology) - (per day)