Supply of Chemicals and Glassware

List of Chemicals required for Biochemistry Practical Laboratory

Acetic Acid (500ml)

Acetone (2.5 Litre)

Acryl amide (500gm)

Agarose, low EEO (500gm)

Alpha-Napthol (500ml)

Ammonium Acetate (500gm)

Ammonium chloride (500gm)

Ammonium Molybdate (500gm)

Ammonium persulphate (10gm)

Ammonium solution (500ml)

Ammonium sulphate (500gm)

Barium chloride (500gm)

Benedicts reagent (500ml)

Benzedine solution (500ml)

Beta-Mercaptoethanol (1 00gm)

Bis Acryl amide (500gm)

Bromocresol Green (500gm)

Bromophenol Blue (10gm)

Butanol (01 Litre)

Citric Acid (500gm)

Coomassie brilliant Blue (10 gm)

Copper sulphate (500 gm)

Diethyl Barbituric Acid (50 gm)

Dipotassium EDTA (100 gm)

Dipotassium hydrogen phosphate (500 gm)

Disodium EDTA (100 gm)

Disodium hydrogen phosphate (500 gm)

DNA Ladder 50 bp (50 μg)

DNA Ladder 100 bp (50 μg)

dNTPs set (25mM)

Dimethyl Sulphoxide (100 ml)

Ehrlichs reagent (500 ml)

Ethanol Absolute (2.5 Litre)

Ether (500 ml)

Ethidium bromide (10 gm)

Ferric chloride (500 gm)

Fouchets reagent 500 ml)

Glycerol (500 ml)

Glycine (100 gm)

Hydrochloric Acid (500 ml)

Hydrogen peroxide (1 Litre)

Isopropanol (1 Litre)

Liquid Ammonia (500 ml)

Litmus paper

Magnesium chloride (500 gm)

Methanol (500 ml)

Ninhydrin (100 gm)

Nitric Acid (500 ml)

O-Toluidine solution (500 ml)

Para-Dimethyl aminobenzaldehyde (500 mgm)

Paraffin Film

pH Tablets (pH 4.0) (60 g)

pH Tablets (pH 7.2) (60 g)

pH Tablets (pH 9.0) (60 g)

Phosphotungstic Acid (500 ml)

Picric Acid (500 ml)

Potassium dihydrogen phosphate (500 gm)

Potassium Oxalate (500 gm)

Proteinase K (10 gm)

Silica Gel (500 gm)

Silver Nitrate (10gm)

Sodium Acetate (500 gm)

Sodium Bicarbonate (500 gm)

Sodium Carbonate (500 gm)

Sodium Chloride (500 gm)

Sodium Citrate (500 gm)

Sodium diethyl barbiturate (500 gm)

Sodium Dihydrogen phosphate (500 gm)

Sodium Dodecyl Sulphate (1000 gm)

Sodium Hydroxide (500gm)

Sodium Hypobromite (500gm)

Sodium Hypochlorite (500ml)

Sodium Nitroprusside (500 gm)

Stannous Chloride (500 gm)

Sucrose (500 gm)

Sulphosalicylic acid (500 gm)

Sulphur powder (500 gm)

Sulphuric Acid ((500 gml)

Taq DNA polymerase with proof reading activity (1000 U)

TEMED (100 ml)

Trichloroacetic Acid (500 gm)

Tris Base (500 gm)

Triton X 100 (1 Litre)

Whatman filter paper grade 1

Whatman filter paper grade 2

Whatman filter paper grade 3

Xylene cyanol (10gm)

List of Glassware/Plasticware required for Biochemistry Practical Laboratory

Beakers-100 ml

Beakers-250 ml

Beakers-1000 ml

Beakers-500 ml

Capillary tubes

Centrifuge Tubes (15 ml) - 100 per Pack

Centrifuge Tubes (50 ml) - 100 per Pack

China Dish

Chromatography Chamber

Conical Flasks-250 ml

Conical Flasks-500 ml

Filter Paper For Paper Chromatograph- 24 per Pack

Glass Funnels

Graduated Measuring Cylinders-1000 ml

Graduated Measuring Cylinders-250 ml

Graduated Measuring Cylinders-500 ml

Graduated Measuring Cylinders-100 ml

Microcentrifuge tube box for 1.5 ml tubes

Micro-Centrifuge Tubes (1.5 ml) – 1000 per Pack

Micropipette Tip box for 10 µl tips

Micropipette Tip box for 1000 µl tips

Micropipette Tip box for 200 µl tips

Micropipette Tips (10 µl) - 1000 per Pack

Micropipette Tips (1000 µl) - 1000 per Pack

Micropipette Tips (200 µl) - 1000 per Pack

Motor And Pestle

Pasteur Pipettes – 100 per Pack

Reagent Storage Bottles With Screw Cap-250 ml

Reagent Storage Bottles With Screw Cap-500 ml

Reagent Storage Bottles With Screw Cap-1000 ml

Spirit Lamps

Test Tube Holders

Test Tube Racks

Test Tubes (20ml) – 100 per pack

Test Tubes (50ml) – 100 per pack

TLC Chamber

TLC Plates

Tripod Stand

Volumetric Flasks-100 ml

Volumetric Flasks-1000 ml

Volumetric Flasks-250 ml

Volumetric Flasks-50 ml

Volumetric Flasks-500 ml

Wash Bottles

Wire Guaze

List of Kits (Kits should be compatible with semi-automatic analyzer) with Methods and Pack Size

Albumin* (Bromocresol Green) (5×25ml) (1×2ml)

ALP* (Paranitrophenyl-p) (425ml) (1×25ml)

Bilirubin* (Diazonium) (5×25ml) (1×2.5ml)

Calcium* (Cal-Arsenazo III) (1×50ml) (1×2ml)

Creatinine* (Jaffe’s method) (4×25ml) (1×25ml) (1×2ml)

CSF protein* (Pyrogalol Red) (6×25ml)

Direct bilirubin* (Diazonium) (5×25ml) (1×2.5ml)

DNA extraction kit from tissue/blood (50 reactions)

Glucose* (GOD-POD) (6×100ml) (1×2ml)

HDL* (PEG – Chod/ PAP (Enzymatic) (2×18.7ml) (6.25×2ml) (1×2ml)

Phosphorus* (Phisphomolybedate) (1×50ml) (1×2ml)

SGOT* (UV-Kinetic Method) (4×25ml) (1×2ml)

SGPT* (UV-Kinetic Method) (4×25ml) (1×2ml)

Standard Amino Acid kit of 24 amino acids

Total Cholesterol* (CHOD-POD) (5×25ml) (1×2ml)

Total Protein* (Biuret) (5×25ml) (1×2ml)

Triglycerides* (Glycerol Phosphate Oxidase) (5×25ml) (1×2ml)

TSH (ELISA based) (96 reactions)

Urea* (Berthelot/Urease) (4×25ml) (1×25ml) (1×2ml)

KALPATRU SCIENTIFIC

MAHAJAN AGENCIES

B M SCIENTIFIC