Purchase of Chemicals and consumables to cytogenetic lab of anatomy dept-

Peripheral blood lymphocyte culture medium 100 ml. Complete medium with PHA

Trypsin – EDTA 0.25% 1* solution, 100ml with PH indicator

Colcemid solution 10µgm/ml in PBS, 10ml or 20ml bottle

Pepsin from porcine gastric mucosa 25gms lyophilized powder for cytogenetic purpose≥2500 units/mg ,Protien– good quality. Sigma/equivalent (E%/280)

Acetic acid – Glacial (High quality) 2.5 litre.

Sodium hydroxide pellets. Good quality, Emplura

Amniotic fluid culture [100 ml] medium – complete media for the culture of amniocytes

Hydrochloric acid – Concentrated

Xylene – (High grade good quality) 2.5 litre

Tween – 20 for molecular biology

Immersion oil – low fluorescence. Type – F – Merck or equivalent quality – 125ml

Measuring cylinder – Borosilicate glass, Graduation mask, transparent – 100ml

Conical flask – glass (graduation marks – 250ml (clear, Borosilicate)

Staining through – glass with lid space to hold 22 slides staining rack, size 15*12*8cm

Pasteuer – pipette – 3ml, caliberated, clear with graduation mask PP – Disposable

Disposable hypodermic Syringe with needle – 2ml (One box with 100 nos.)

Centrifuge tube – sterile 15ml. Good quality (Tavson or thermoscientif equivalent) with clear graduation marks.

1.5 ml microcentrifuge tubes with snap cap, P. P, graduated, clear, sterile

Microscopic slides, 75mm*25mm. Good quality, lint free, non greasy, ground edges, interleaved.[50 no.s in 1 box]

T20 culture flask with filter cap – sterile, growth area 20cm2

PH paper strips – Range (1-7) (7-114)-10 books per box

Sterile powder free gloves Size – 7

Sterile powder free gloves Size 6.5

Tissue paper rolls. Good quality

Nitrile gloves - Size – 7

Nitrile gloves -Size – 6.5

Coverslip small – round 18*18

Coplin jars (PP). Autoclavaple – 4 groves

Sterile filter tip (Barriertip ), Free from DNase, RNase, endoteriing (universal type). Graduated clear 96 tips in one box

Staining rack (steel) with handle (22 slides slots)

Microscopie slide. Maker – plastic – slot for 8 -10 slides