Rate contract for carrying out Toxicological studies from Good Laboratory Practice (GLP) certified laboratories of Nano-fertilizer developed by R and D (BioAgro) department, RCF.

Amount

Human health safety (ENV/JM/MONO (2018) 4): IATA for skin corrosion and irritation (ENV/JM/MONO (2014) 19)

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 428 - Skin absorption: In vitro method

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 431 - In vitro skin corrosion: Reconstructed human epidermis (RHE) test method

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 437 - Bovine corneal opacity and permeability test method for evaluation of chemicals likely to cause serious eye damage

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 433 – Acute inhalation toxicity for evaluation of health hazard from short-term exposure to a test chemical by inhalation.

Human health safety (ENV/JM/MONO (2018) 4):OECD 436 - Acute inhalation toxicity: acute toxic class method

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 473 - In vitro mammalian chromosomal aberration test

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 476 - In vitro mammalian cell gene mutation tests using the HPRT and XPRT genes

Human health safety (ENV/JM/MONO (2018) 4):OECD TG 487 - In vitro mammalian cell micronucleus test

Human health safety (ENV/JM/MONO (2018) 4):ATP Cell Titer-Glo, neutral red uptake, LDH release, MTT, XTT, cell impedance, trypan blue, BrdU, Alamar Blue, WST-1, live/dead cell counting, colony-forming efficiency

Human health safety (ENV/JM/MONO (2018) 4): In addition, Test No. 489 - In vitro mammalian alkaline comet assay for measuring DNA strand breaks in eukaryotic cells may also be included in the test panel.

Environmental safety:OECD TG 201 - Aquatic test: fresh water algae, cyanobacteria and growth inhibition test

Environmental safety:OECD TG 202 - Daphnia sp. acute immobilization test

Environmental safety:OECD TG 203 - Fish acute toxicity test

Environmental safety:OECD TG 211 - Daphnia magna reproduction test

Environmental safety:OECD TG 236 - Fish embryo acute toxicity test on embryonic stages of fish (Daniorerio).

Environmental safety:OECD TG 208 - Terrestrial plant test: seedling emergence and seedling growth test U.S. Environmental Protection Agency (US EPA) - Six bioassays: Allium and Vicia root tip chromosome breaks, Tradescantia chromosome break, Tradescantia micronucleus, Tradescantia stamen-hair mutation, and Arabidopsis mutation bioassays - for detecting the genotoxicity of environmental agents (Ma et al., 2005).

Effects on soil microbiota and macrobiota:OECD TG 222 – Earthworm reproduction test for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei

Effects on soil microbiota and macrobiota:OECD TG 216 - for the long-term effects after a single exposure of chemicals, on nitrogen transformation activity of soil microbiota

Effects on soil microbiota and macrobiota:OECD 217 – to investigate long-term effects after a single exposure of agrochemicals/ nonagrochemicals on carbon transformation activity of soil microorganisms

Effects on soil microbiota and macrobiota:OECD TG 471 - Bacterial Reverse Mutation Test. Additionally, Clinical and Laboratory Standards Institute, methods 02, 07, and 11 may be adopted for in vitro evaluation of antimicrobial susceptibility of NAPs and NAIPs. Suitable standard guidelines to evaluate risk to macrobiota may also be developed and provided by regulators. Suitable evaluation method may be included to assess effect of NAIPs on the alteration of biotic and abiotic soil properties. The outcome of toxicity tests may be used to establish thresholds or safe levels of chemicals in soil. Methods for estimation of the half-life of the NMs in soil may also be included to predict (potential) risk.

Effects on soil microbiota and macrobiota:OECD TG 307 may be adopted with necessary adaptations for aerobic-anaerobic transformation of NAIPs or NAPs in soil.

Effects on soil microbiota and macrobiota:OECD guidelines for the testing of chemicals, Section 3: Test No. 318 may be adopted with modifications for NAIPs and NAPs.

Packaging compatibility data: Decision framework (OECD, ENV/JM/MONO (2019)12) for inclusion of physico-chemical parameters for exposure and fate assessment of NAIPs.

Packaging compatibility data:Avain dietary toxicity(OECD205)-5 crop samples

Packaging compatibility data:28-day repeated dose toxicity(OECD407)-5crop samples

Packaging compatibility data:Leaching study (OECD 312)-3 type soil and one plant

Packaging compatibility data:Nutritional analysis-5 crop samples

Bioscience Research Foundation