Supply, Installation and commissioning of FPLC Protein Purification System

Supply, Installation and commissioning of FPLC protein purification System as per technical specifications at Annexure 7

Software: At least two additional offline evaluation licenses should be supplied for the data analysis and evaluation software on other computers

For large samples more than 10 mL volume, larger loops (one for 10 to 25 mL) capable of loading integral small volumes multiple times should be provided.

Columns and column holders to be provided as optional items that are compatible with the main system:

Strong cation, weak cation, strong anion, weak anion exchange columns of 5 ml and 10 ml size (1 each).

Size exclusion preparative (~120 mL bed volume) and analytical grade columns (~20-25 mL bed volume) both capable of separating macromolecules of size ranges 10-600 kDa (one each). Another Size exclusion preparative (~120 mL bed volume) column (made of chemically modified allyl dextran and N,N′-methylene bisacrylamide co-polymer) for separating antibodies, serum proteins and proteins of size 10 to 1500 kDa (one quantity).

Affinity columns: High affinity MBP tag of volumes 1 and 5 ml and GST tag of volumes 5 and 20 ml (one each).

Hydrophobic interaction (5 mL), calmodulin binding resin (5 ml) and IgG sepharose (5 mL) (one each).