Service of Whole transcriptome sequencing of human cell lines using illumina Novaseq 6000 with data analysis

Availing Service of Whole transcriptome sequencing of human cell lines using illumina Novaseq 6000 with data analysis

Service of Whole transcriptome sequencing of human cell lines using illumina Novaseq 6000 with data analysis. Specification:- 1. RNA integrity and Quality Check using Bio analyzer, Gel electrophoresis, nanodrop and Qubit 2. High quality data with a minimum Q30 reads 3. Minimum Data output: 16GB per sample. 4. Sequencing after mRNA enrichment with a Read Length of 2 x 150 bp 5. Bioinformatics analysis: • Primary data analysis including quality check, filtering, and trimming • Calculation of data statistics such as coverage, N content, and identification of poor quality reads. • Alignment statistics ensuring a minimum alignment of 80-90%. • Implementation of both reference-based and de novo assembly approaches. • Comprehensive analysis of splicing patterns and related gene functions. • Differential gene expression analysis among samples and different groups • Gene annotations and core gene set analysis. • KEGG Pathways analysis using multiple databases. • Provision of heatmaps ad BAM files for each comparision. • Training sessions for data interpretation (for the data generated). • Submission of detailed analysis reports for each step and data submission in NCBI.