Filed and Lab Operastions in Division of Genetics and Seed Science

Amount

Wheat

Sowing and covering of seeds in 4 m row

Dibbling/seeding manually in plot(2 rows x 5 m)

Emasculation and pollination

Recording data (no. of tillers, plant height, spike length, peduncle length, spikelet no./spike,filled grains/spike.)

Recording biological yield of 1m row

Number of tillers per meter

Single spike threshing

Single plant harvesting & threshing

Harvesting and threshing one meter row length

Harvesting and threshing per plot (1.38m x 5m)

Harvesting and threshing per plot(6m x 1.2m)

Harvesting and threshing of plot(0.45x 5 m)

Wheat Crop residue spread

Biomass assessment in wheat Plot 6 X 1.5m

Mannual weeding in wheat

Preparing wheat seed for off season nursery 50- to 100gm sample

Cleaning of seed after harvest (per 6x1.5m Plot)

Weeding with kasola - Kharif season (Wheat) Crop)

Weeding with khurpi - Ravi season (Wheat )

Weeding with kasola - Kharif season (Wheat Crop)

Weeding with khurpi - Ravi season (Wheat Crop)

Maize

Sowing and covering of seed in 3 m row

Silking per plant

Tasseling per plant

Separation, weighing and de-husking of green cobs per 5m row

Harvesting, collection and packing of cobs of single row of 3 m

Separation of cobs from plants

De-husking of cob in 3 m row

Separation of kernel from cob

Drying of cobs and seed on daily basis

Harvesting of baby cob per picking per 3 m row

Data recording i.e. plant stand, plant height/ cob height / no of cob, kernel row , no of kernel, cob length , cob girth, 100 kernel weight (per 3m row): 2 man-days for 100 rows is needed

Biomass weight per row of 3 m

De-tasseling during seed production

Harvesting male line/acre in seed production plots

Harvesting female line in seed production plots

Maize crop residue spread

Maize residue chopping

Manual weeding Maize

Recording of baby corn parameters VIZ. Length, width,5 cob weight

Weeding with kasola - Kharif season (MaizeCrop)

Weeding with khurpi - Ravi season (MaizeCrop)

Weeding with khurpi - Khrif season (MaizeCrop)

Weeding with kasola - Ravi season (MaizeCrop)

Preparation of nursery beds for sowing (bed size 4x3 m2)

Preparation of bunds in main field (bund width of 75 cm each)

Making furrow(1 m each ) for nursery sowing of single plant

Nursery sowing and covering with soil (1 m each)

Nursery weeding (bed of 4x3 m2)

Nursery uprooting (1 m each)

Shifting of nursery to main field

Manual leveling of field before transplanting

Field layout before transplanting

Transplanting of trials (12 m2plot)

Handling of rope during transplanting

Transplanting of bulk material

Fixing of isolation sheet in hybrid seed production plot (Digging 1 feet deep hole, fixing bamboo in it and stitching isolation sheet on it)

Transplanting of CMS multiplication/hybrid seed production plot

Transplanting of breeding material (SP progenies)/germplasm / RIL’s

Cleaning and maintenance of bunds in main field

Rouging in CMS multiplication/ hybrid seed production plot

Rope pulling in CMS multiplication/hybrids seed production plot

Spraying of GA3 using knapsack sprayer

BLB inoculation

Recording of BLB lesion length (5 leaves per plant)

Hand emasculation and pollination in normal varieties

Hand emasculation and pollination in CMS lines

Spraying of insecticides/herbicides/fungicides

Harvesting and threshing of single plant

Collection of panicles from plots/hills

Recording single plant data on yield and yield components(tiller number, plant height, panicle length)

Counting of filled grains/panicle

Cleaning and drying of seeds of trial

Harvesting and threshing

Bulk harvesting and threshing

Removal of isolation sheet and bamboo

Mixing of B/R lines seedlings (different dates) before transplanting

Irrigations in nursery bed 4x3m2

Application of micronutrient solution in nursery bed using knapsack sprayer(bed size 4x3m2)

Removing of flouting crop residues/debris in puddled main field before transplanting

Weeding in (Rice Crop)

Pearl millet

Opening the furrow with plough, sowing and covering the seed per 3 m row

Selfing

Emasculation and pollination

Screening of male fertile/sterile plants of 3m row (@20 plants per 3m row)

Data recording (includes plant height, spike length, spike thickness, no. of tillers)

Harvesting of spike per 3m row

Harvesting of plants from base and removal from field of 3m row

Harvesting bundling and taking the spikes out

Weighing of spikes and fodder of 3m row basis

Threshing single ear head basis

Threshing row basis (3m row)

Removal of spikes and their threshing

General harvesting of spikes

Weeding with kasola - Kharif season Pearl millet)

Weeding with khurpi - Ravi season (Pearl millet)

Weeding with khurpi - Khrif season (Pearl millet)

Weeding with kasola - Ravi season (Pearl millet))

Chickpea, Moongbean, lentil, soybean

Emasculation and pollination

Harvesting & threshing single row of 2 m length

Harvesting & threshing single row of 5m length

Preparation of Seed packet for sowing

Harvesting and threshing single plant

Harvesting & threshing of plots (5m x1.5m)

Harvesting and threshing of plot (5m x 2.1 m plot)

Recording data on single plants (Plant height,counting primary and secondary branches, pods, seeds per pod)

Harvesting and Threshing - general

Weeding with kasola - Kharif season (Pearl Chickpea, Moongbean, lentil, soybean)

Weeding with khurpi - Ravi season (Pearl Chickpea, Moongbean, lentil, soybean)

Weeding with khurpi - Khrif season (Chickpea, Moongbean, lentil, soybean)

Weeding with kasola - Ravi season (Chickpea, Moongbean, lentil, soybean)

Pigeon pea

Emasculation and pollination

Selfing

Selfing of individual inflorescence by selfing bags

Screening of male fertile/sterile plants in 3 m row

Plot harvesting and threshing (4.0m x 1.8 m plot)

Plot harvesting and threshing (4.0m x 5.0 m plot)

Harvesting, threshing and cleaning of 5 m row

Harvesting, threshing and cleaning of 3 m row

Harvesting, threshing and cleaning of single plants

Single plant field observations (Plant height, primary & sec branches, main shoot length, Pod length, Seeds per pod, 100 Seed wt, Plant type-DT/IDT/erect /Spreading, Flower colour and Seed colour etc)

Weeding with kasola - Kharif season (Pigeon pea)

Weeding with khurpi - Ravi season (Pigeon pea)

Weeding with khurpi - Kharif season (Pigeon pea)

Weeding with kasola -Ravi season (Pigeon pea)

Mustard

Emasculation and pollination

Selfing single plant with three ring selfing bags supported with bamboo sticks

Selfing of Brassica inflorescence with selfing bags

Harvesting and threshing of single row of 5m

Single plant field observations (Plant height, primary & sec branches, main shoot length, siliquae on main shoot, seeds/siliqua, siliqua length, point to first branch)

Erecting pipes for selfing (In 50 cm deep hole for 1.5” pipe) + putting pipes on in 20” x 20” square

Putting nets on pipes (10 m x 5 m x 3 m)

Inoculation for white rust/Sclerotinia stem rot

Single plant harvesting and threshing

Plot harvesting and threshing (5 m x 1.5m) plot after removing border row

Plot harvesting and threshing (5 m x 2.7m) plot after removing border row

Weeding with kasola - Kharif season Mustard)

Weeding with khurpi -Ravi season (Mustard )

Weeding with khurpi - Khrif season (Mustard)

Weeding with kasola - Ravi season (Mustard )

Common field work

Field layout

Plot sowing by hand plough

Plot sowing with tractor mounted machine

Marking lines with marker in the field

Dusting (manually) of crop with pesticide

1000 seed counting (Mustard and pearl millet)

100 seed counting in all crops

Seed treatment per packet (50-200 g)

Preparation of seed packets

Hand cleaning of seed

Hand cleaning of seed samples (50-200g)

Fixing bamboo/plastic sticks for labeling

Tying of labels on plant/sticks

Selfing of single plants with net bag supported with bamboo

Selfing of plots- size 3 m x 3.6 m with net bag supported with bamboo

Hand weeding in field

Weeding of net houses 400 sqm area

Cleaning around the net houses

Deep digging net-house 6 inch deep

Path and bund cleaning

Bird scaring per 5 acre plot (two shift)

Bird scaring per 4 acre plot (two shift)

Bird scaring per 3 acre plot (two shift)

Bird scaring per 2.5 acres plot (two shift)

Bird scaring per 2 acre plot (two shift)

Bird scaring per one acre plot (two shift)

Bird scaring in pearlmillet and maize at maturity(two shift)

Bird scaring in pearlmillet and maize at maturity(two shift)

Bird scaring in pearlmillet and maize at maturity(two shift)

Irrigation

Harvesting and threshing with machines

Bulk threshing of crop - General

Bulk harvesting of crop- General

Harvesting and threshing of crop -General

Cleaning of harvested seed in field / lab

Gap filling by transplanting

Rouging

Thinning (mustard, rice and pearl millet, maize, wheat)

Thinning (soybean, chickpea, mungbean, pigeon pea, and other crops)

Spreading of FYM in the field

Application of fertilizers (all crops)

Hill application of fertilizers/granules

Preparation of sticks/ pegs (90 cm) from raw bamboo

Spray of pesticides with tractor sprayer in field

Spray of pesticides with knap sack/foot pump sprayer in field

Removal of crop residue out from the field -General

Threshing of single spike in wheat/panicle in rice

Roughing in Nucleus/Breeder Seed Plots

Transplanting of rice for nucleus seed

Transplanting of vegetables

Preparing beds (flat/ridge) for vegetable sowing/ transplanting

Field emergence, speed of emergence and plant stand count of 5 meter row length

Inoculation of plants

Bulk/single plant observation of DUS characters (Ten traits)

Common lab,field work across crops , seed testing activities and pot culture etc

Sample preparation of leaf/seed for quality analysis

Tagging of single plants and collection of leaf samples in liquid nitrogen

Plant-wise harvesting of genotyped pollinator plants

Grinding of samples for DNA isolation (5 g leaf)

Preparation of glassware and plastic ware for RNA work through NaOH treatment DEPAC water treatment sterilized through autoclaving

Sample preparation for enzyme/hormone analysis

Washing and autoclaving of mortar-pestle and glass and plastic wares

Air drying, grinding and sieving of soil samples in the laboratory

Bread making 100 g sample in wheat

Biscuit making 500 g sample in wheat

Chapatti making 300 g sample in wheat

Help in sedimentation analysis in wheat

Brix value estimation/cob for sweet corn/green cob (Maize)

Estimation of popping value (200 kernels per sample) in maize

Estimation of kernel opaqueness through light box (200 seeds/sample) in maize

Grinding of maize seed (50-100 seeds) samples for biochemical estimation

Analysis of pollen fertility in all crops

Hulling by palm husker of 25 grains/sample in rice

Hand milling of 25 grains/sample in rice

Grinding of rice grains of 4g/sample

Hulling and milling of trial samples of 200g paddy in machines

Preparation of samples and data recording of rice grain dimensions (kernel length, width) before and after cooking

Separation of head rice from milled rice samples obtained from 200g paddy samples

Filling of pots with soil for sowing

Filling of pots and shifting plants in pots

Filling of pots (4")

Sowing in pots (4")

Filling of trays (10" x 5" x 3")

Sowing and planting in pots 12 inch x 14 inch pots

Empting of pots all sizes

Sowing in tray 9 x 4 inch tray

Preparation and sowing of seeds in plastic cups

Pot filling and mixing of soil with FYM 12 inch x 14 inch pots

Weeding and inter-culture in pots

Irrigation in pots

Laboratory analysis of soil and plant samples

Collection of soil samples from the field, mixing and transporting

Collection, processing and grinding of plant samples

Sample preparation for physical purity

Seed purity analysis

Media (Substratum) preparation towel paper (BP), Top of Paper (TP), sand and/or Agar medium

Seedling evaluation

Planting of samples in different substratum for seed germination

Preparation of seed samples for electrical conductance

Reading of electrical conductance from soaked seed

Preparation and conditioning of seed samples for accelerated ageing test (AAT) under controlled environment (400C, 100% RH)

Preparation of seed samples for seedling growth

Seedling measurement

Preparation and conditioning of seed samples for tetrazolium test (TZ)

Evaluation of embryo staining

Filling of tip boxes and sterilization

Preparation of samples for germination test

First count during germination test

Final count during germination test

Root and shoot length observations on 10 seedlings from each sample

Dry weight observation on 10 seedlings from each sample

Preparation of solutions/chemicals for seed treatment

Treating the seed with various chemicals/coating priming etc.

Plating of fungi

Counting 100, 50 and 25 per seeds sample

Cleaning of seed and leaf samples

Maintenance of temp. and humidifier in the walk-in germination chambers

Storage and upkeep of guard samples

Moisture content estimation

Maintenance and upkeep of glass house ,Ne House and surrounding area (100 m2)

Temperature and humidity controlled seed store cleaning of chillers unit, water storage tank clean & cleaning of filters fitted in Dehumidifiers and cooling coil, cleaning of racks of seed storage room

Cleaning/Dusting of machines–seed dryer, seed air and screen cleaner, gravity separator, seed coating, in the process. lab.

Maintenance and up keeping of Net House and Surrounding area 100sq meter

Preparation of Fly food and subculture

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