Supply of Scientific Equipment to Centre of Excellence

Equipment for Centre of Excellence for Environment

Automated capillary electrophoretic DNA sequencer Should be a fully automated Multi-capillary, fluorescence-based genetic analysis system and must have: 1. The system should be 4-capillary fluorescence-based capillary electrophoresis system. 2. The system must be able to detect and analyze 6 fluorescent dyes simultaneously. 3. The system should have solid-state long life laser. 4. The system should use integrated cartridge which includes polymer, capillaries and anode buffer. 5. The system should use universal performance optimized polymer to run both sequencing and fragment analysis applications. 6. The system software should support to run sequencing and fragment analysis application on the same plate. 7. The system should use RFID for tracking and reporting of the consumables. 8. The system should be stand-alone instrument with touch screen interface for computer independent operation. 9. The system should contain on-board storage capability with memory capacity of at least 128 Gb for storing approximately 3,500 injections or 14000 reactions. 10. The system touch screen should have functionality to create and run protocols; load run files from USB or an online ecosystem; to designate destination of run files to the USB port or an online ecosystem; pause and resume the run in progress and allow users to edit sample and target assignments. 11. The system software should be designed with a touchscreen software interface for instrument control to regulate the functions of the instrument, automatically perform primary analysis (i.e, base calling and/or fragment sizing) and provide functionality for real-time data assessment as well as the ability to generate quality control reports. 12. The system should have Plate Manager Software that allows for run set-up via the on instrument software, Cloud or personal computer. 13. The system should be cloud-enabled to set up run, check its progress, and access data. 14. The system should be capable of doing sanger sequencing applications (re-sequencing NGS confirmation, indels; heterozygote detection; minor variant detection ) and fragment analysis applications (microsatellite analysis, compatible with MLPA, Snapshot, cell line authentication ) 15. The system should come with secondary analysis software to base call, trim, display, edit and print data. 16. The system should come with secondary analysis software for resequencing, mutation detection and analysis, SNP discovery and validation, allele identification and subtyping. 17. The system should come with software designed for reference-based and non-reference based analysis to call SNP’s, insertions and deletions . The system should have software for accurate detection and reporting of minor variants with detection level as low as 5%. 18. The system should come with genotyping software that provides DNA sizing and quality allele calls. 19. The vendor should attach at least 50 user list and 5 satisfactory report for Genetic Analyzer 20. The vendor should have fully functional lab for providing the efficient after sales and service support. 21. A dedicated Desktop with latest configuration should be supplied along with the System. 22. Consumables and cartridges for running 1000 samples should be quoted along with the System. 23. 3years warranty/CMC and 2years extended AMC statement were expected

Real Time PCR system A dedicated multicolor Real Time PCR system (excitation and emission) with latest generation Peltier-based 96-well plate/tube in-built PCR to support: a. Gene-Expression analysis, b. Pathogen Quantitation, c. SNP Genotyping, d. Plus/Minus Assays that utilize internal positive control, e. Dissociation Curve Analysis, f. Multiplexing and complete End-Point Assays. g. MicroRNA profiling. 2. LED Light Source for excitation. 3. Machine should have 3 coupled filters for Optical Detection. 4. Multiplexing should be minimum 3 targets/well. 5. Machine must have 2D barcode reading facility(Optional) 6. Peltier base Heating and Cooling Method7. Temperature/Method optimization : Isothermal 8. Imaging System : CMOS/CCD/Photodiode9. Dynamic Range : 10 Log 10. Temperature uniformity : 0.4 °C 11. Temperature Accuracy : 0.25°C 12. System should have Block ramp rate : 3.5°C or more 13. Run Time should be less than 45 Mins 14. Number of Copies : 1 copy 15. Sensitivity : Detect differences as small as 1.5-fold in target quantities in singleplex reaction 16. System should be wifi enabled to access and share data anywhere and anytime. 17. Total reaction volume range 10 ulu to 100 uL for 96 well plate. 18. Computer: A business line computer (Desktop) with latest computer specifications should be provided with the system. 19. The amplification traces viewed on the LCD screen in real time while a run is in progress with touch screen facility. 20. The supplier should be able to supply all the reagents and consumables for the operation of the system 21. Along with Installation, Extensive Training must be provided. 22. Programmable pause and power interruption recovery options should be available. 23. Pin protected multiple user accounts for data safety and locked templates should be available. 24. Application software like dedicated primer and probe design software as well as relative quantitation analysis software to analyze multiple 96-well-plates of data simultaneously must be included as standard supply in the quoted price – (Optional). 25. Factory calibrated system for quick setup. 26. The vendor should have minimum 5 installations in the past 3 years. 27. A dedicated Desktop with latest configuration should be supplied along with the system with optical R/W drive. 28. 3years warranty/CMC and 2years extended AMC statement were expected

MODULAR QUATERNARY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) SYSTEM. HPLC system consisting of Quaternary Gradient pump, Auto Sampler, Online Degasser, Column Oven, Diode Array detector, software and Accessories. QUATERNARY GRADIENT PUMP:4-Channel facility in pump with serial pistons, Able to work as Isocratic, Binary, Ternary and Quaternary mode., Automatic Gradient Profiles facility Proportionating Precision: ± 0.15% or less , Proportionating: 5 to 95% , Proportionating Accuracy: ± 0.5% or better, Operating flow range: 0.01 - 10.00 ml/min, in 0.01 increments Operating pressure Range : 620 Bar Maximum or better for the entire range, Flow Precision: 0.1% RSD , Flow Accuracy: ± 1%, Degasser: Inbuilt on line degasser with 4 channels for solvent degassing. Maximum operating flow rate should be up to 10mL/min or more per flow line, Photo Diode Array Detector:, Photodiodes: 1024 diodes, Band width: 4 nm or lesser, Wavelength Range: 190-800nm Light source: Pre-aligned Deuterium lamp and Tungsten Lamp, Wavelength Accuracy: ±1 nm or better, Digital resolution: <1 nm/ pixel, Linearity range: < 5% RSD and corr. coeff. ³ 0.999 up to 2.0 AU Base line noise: ± 8µAU or better, Drift: ≤ 1.0x10-3/AU/Hr or better, Path Length: 10 mm , Flow Cell Volume: ≤ 13ul or equivalent, Data acquisition rate: 100 Hz or better, Peak Purity, Peak scanning facility to visualize any difference in spectra to find out multiple up to 4 co eluting impurity with retention time. Software : To control, acquisition, online display, processing peak point integration and reporting HPLC data, Full 64 - Bit Architecture software, Windows 10/8 compatible environment or suitable, Data reports, online help and wizards, Data Integrity, Advanced Security, Audit Trials, System suitability min 5 Parameter can be checked , Template saving & Auto run of templates , Calibration curves facility System suit formula facility , Facility for data security, audit trails and electronic signatures etc., should be available for GLP and 21 CFR compliance. Single point control of the entire HPLC., IQ OQ of the HPLC and Software system must be done. , Columns (Pore Size 5 µ) :, (i)C-18 Column : 1 Nos. _250x4.6 mm, 5u Suitable PC & Printer with below specifications to be supplied: Processor - Intel i5 (5th generation) ; RAM - 8 GB ; Hard disk - 1 TB ; Graphic Card; DVD writer; 17” TFT screen ; LAN Port ; USB 2.0, 4 Ports ; Wi-Fi ; Multimedia Keyboard ; Optical Mouse, Rewritable DVD, Operating System : Genuine Windows 10 ultimate with media and documentation and certificate of Authenticity. Preloaded latest Licensed Antivirus Software. Printer- Laser Printer Monochrome., Warranty : 3years warranty/CMC and 2years extended AMC statement were expected , All necessary accessories for the full Fledge working of the instrument to be quoted, The supplier should have installed minimum 5 Nos of the quoted model in Kerala preferably , In government institutions. Installation details to be enclosed along with the contact details. The supplier should have service facility in kerala to render prompt after sales service.

LAMINAR AIR FLOW -VERTICAL with CE Mark 1. Basic elements: Prefilters, heavy duty blower assenbly, Hepa filter etc. 2. Filteration Process: Fresh air is drawn through the Pre- filter and passes through Hepa filter having efficiency rating as high as 99.99% with cold DOP and 99.97% with hot DOP, thus retaining all airborne particle of size 0.3 micron and larger. 3. Construction: Made of good quality sheet steel with steel reinforcements all powder coated. 4. Filteration: Hepa filter deep pleat of high efficiency and quality marked like CE 5. Work Table Top: High quality Stainless Steel matt finished 6. Side Panels: Made of good quality melamine/Acrylic 7. Blowers: Noiseless high quality blowers 8. Air Velocity: 90ft/min UV tube Manometer Gascock Chamber Size:4' X 2' 9. Certifications : 1. CE certified 2. ISO 13485: 2003 (Medical devices) 3. ISO 9001: 2015. 10. 3years warranty/CMC and 2years extended AMC statement were expected

CO2 Incubator 1. The incubator chamber is constructed entirely of polished, corrosion resistant stainless steel with coved corners to simplify cleaning and decontamination practices to minimize the potential for unwanted contamination. 2. Lightweight direct heat design surrounds sides, back, top, and bottom of chamber with high efficiency, quick response heating elements. 3. Large interior chamber capacity of above 180 liters , 4. Sturdy modular stainless steel shelving and supports which can be readily assembled and removed without use of tools. 5. An in-chamber HEPA filtered airflow system within the culture environment, continuously filters the entire chamber air volume every 60 seconds 6. Superior Class 100 clean room air quality is achieved within the culture chamber in under 5 minutes after a door closing. 7. High temperature sterilization function with exposure to 140°C, to eliminate bacteria, mold, yeast, mycoplasma and even resistant spores, within a 12 hour total process cycle. 8. The incubators on-board contamination control technologies have been validated by independent third party testing facilities and documented in published works. 9. An exclusive filtered air exchange system within the incubator minimizes the risk of condensation. A heated outer door design facilitates quicker recovery to desired set temperature following door openings while minimizing resultant condensation found on the glass of incubator. 10. The unit incorporates high quality microbiological filters on all gas inlets, outlets, and sample ports. The incubator should be capable of being supplied with a choice of either highly stable and long lasting TC gas sensor or enhanced sensitivity IR sensors. 11. Unit incorporates an advanced microprocessor based control and monitoring system featuring an easy to navigate alphanumeric messaging center, with individual displays for temperature, C02, to simplify observation from a distance and ease of operation for all users. 12. All control and measurement probes and sensors are located directly inside the culture chamber to provide true and accurate process data and foster faster parameter recovery times than possible with remotely located sensors outside the chamber. 13. Incubator should provide field reversible inner and outer doors, simplifying choice of preferred configuration for use in any laboratory environment. 14. Units are readily stackable with easy to install hardware provided as standard, to insure stability of the stacked configuration while maximizing available lab space. 15. Incubator is equipped with programmable tracking alarms for critical temperature and CO2 control parameters, which can be custom configured to meet the needs of changing research requirements. The unit incorporates a convenient automatic electronic start up function complete with auto zeroing capability to assure proper calibration without time consuming manual measurement and adjustment, prior to loading with cultures. 16. Incubator is UL listed, CSA certified, and CE marked. 17. 3years warranty/CMC and 2years extended AMC statement were expected

Automatic weather monitoring station Meteorological Sensors/Measurements: 1. Air Temperature Sensor : Range - 40 °C to + 65°C Accuracy ± 0.1° Resolution 0.1° C Units of Measure °C. 2. Relative Humidity Sensor Parameters: Values Range 0 to 100% RH Accuracy ± 2 3. Barometric Pressure Sensor Parameters: Values Range 650 – 1100 hPa Accuracy ± 0.3 hPa. Anemometer (Wind Sensor) Parameters: Values Range (Wind Speed) 0.5 to 60 m/sec Accuracy ±0.3 m/sec (≤ 10 m/sec) and ± 2% (≥ 10 m/sec) Resolution 1.0 unit Units of Measure Wind Speed: m/s and km/h. 4. Wind Direction: Range 0 – 360° Accuracy ± 0.1° Units Displayed East, West, North, South, N-E, N-W, S-E & S- W. 5. Rain Fall Sensor Parameters: Values Type Size: Professional Quality Tipping Bucket Rain Gauge or Impact Sensor or Optical Sensor Accuracy 0.1 mm Resolution 0.25 mm precipitation per measurement Units of Measure Rain-rate in mm/hr and Rainfall accumulation Measurement Data Rate Configurable Rate (1s, 10s, 61m etc.) 6. Data Collection Platform: GPS based Digital Data logger with inbuilt memory and support for using external memory like Pen drive. Data Storage for minimum 30 days and Data backup facility Data logger should provide site location and timing information. 7. Output Interface: RS232/RS422 and Ethernet 8. Power Supply: 210 to 240VAC, 50 Hz 9. Battery Backup: Internal Battery of sufficient capacity to be provided for minimum backup of 12 hours. Battery Charging/Regulator with Charge/Recharge control and Deep discharge protection to be provided. 10. Operating Temperature -35°C to +70°C 11. Protection Class IP65. 12. Operating Humidity 0% to 100% humidity 13. Enclosure Material Acid-Proof, Rust-Proof and UV stabilized. 14. Sensor Module Software: Vendor shall provide real-time display software. It should be capable of retrieving the data from the solid state recorder and present the data in Excel Importable format. The software should also generate reports (Daily/Weekly/Monthly and Yearly) in Word or PDF format. General Instructions: Vendor shall provide initial installation and setup of the equipment. Necessary Interface and Data cables to be provided. 15. Direct and diffused Solar flux detectors 16. Soil temperature and water content sensors up to a depth of 5 to 10 meters 17. Onsite demo and training should be provided 18. 3years warranty/CMC and 2years extended AMC statement were expected

Synergy Technical Systems

Scientific Enterprises

MICROCOMM INDIA LIMITED

Vijay Kumar Enterprises