INVITATION OF TENDER FOR PROVIDING SERVICES TO ACCOMPLISH LABORATORY PROCEDURES AT INTEGRATED BIOCENTRE MYSURU

Contaminated/ Used test tubes/ petriplates are taken out of the MUSHROOM laboratory and cleaned with brush, hot water, distilled water, Cleaned test tubes/plates are wrapped in paper and placed in PP cover, sterilized and oven dried(no)

Cotton plugs are prepared by cutting the cotton which is suitable to test tube mouth (no)

Measurement of Chemicals required for media preparation. Preparation of Media solution. Prepared media is filled to conical flask/test tube and sterilized in autoclave (skilled work) (no)

Sterilized media is taken from autoclave and required amount of media is poured into Petri plates. Test tubes are kept in slants (no).

Inoculating the media with pure culture/ sub culture (no) (skilled work)

Required bottles for spawn preparation are washed with brush, hot water and then sterilized in autoclave. (no)

Cotton plugs are preparation which is required for commercial spawn and PVC ring preparation. (no)

Cleaning of white jowar seeds, boiling , mixing with chalk powder and filling in pp cover/sterilized bottles, plugging with cotton, loading in autoclave for sterilization, after sterilization bottles/ PP cover are kept in inoculation chamber under laminar air flow for inoculation. ( 1Kg packet/bottle)

Inoculating of Mother spawn bottles under laminar air flow and incubation in BOD Incubator (no) (skilled work)

Inoculation of generation-1/generation-2 spawn bottles and placing for incubation in BOD incubator (no) (skilled work)

Inoculation of commercial spawn packets and incubation in BOD incubator (no)

Dry paddy straw is cut into 2.5 to 3 inches, soaked in cold water and shade dried. (kg)

Optimally dried paddy straw are filled in PP cover, Mouth of PP cover are tied using PVC ring and stuffed with non absorbent cotton and kept in autoclave for sterilization (no)

After sterilization bags are taken out of autoclave and allowed for cooling of bags, bags are inoculated with spawn and placed in spawning room for incubation(no)

Oyster Mushroom , after spawn run the bags shifted to cropping room for cropping (no)

Milky Mushroom , preparation casing mixture(kg)

Milky Mushroom after completion of spawn running, casing layer is applied on bags and bags are kept in cropping room

Maintenance of bags in cropping room(spraying of water/relative humidity/temperature/etc) (no)

Harvesting of cultivated Mushroom and packing. (kg)

Maintenance of cleanliness in and around the laboratory, Periodic washing and cleaning of lab and other related activities

Regular maintenance of Grain cleaner, Grain boiler, Mixing cum filler Machine, Autoclaves, Hot Air Over and Other equipment

spraying/ fumigation in the lab with disinfectants (one)

Inspection and examination of inoculated test tubes/ petriplates. bottles of various generation/ commercial spawn for contamination (skilled work)

washing of contaminated test tubes/petriplates/ bottles, sterilizing them and drying in hot air oven

Transferring of Contaminated Spawn packets/RTF bag and spent Mushroom substrate to composting pits

To analyze pH and EC, Samples are mixed with water, Calibrate the EC and pH meters with buffer solution and record the readings

To analyze particular microorganisms, prepare the solution using weighing certified chemicals. For serial dilution, required test tubes, petriplates and media which are to be autoclaved is prepared.

The Glass wares used for the analysis is washed once in water, rinsed in clean water and finally rinsed in distilled water.

Preparation of subculture-I weigh the certified chemicals and prepare the media for required micro organisms and autoclave. Cut the cotton and tie it to the test tubes and flasks. Inoculate the media by using prepared tubes and pertiplates under laminar airflow. Seal it with cling wrap

Preparation of subculture-I weigh the certified chemicals and prepare the media for required micro organisms and autoclave. Using autoclaved test tubes, Petriplates and prepared media Inoculate the culture to laminar air flow. To prepare sub culture for different micro organisms. weigh the required chemicals and prepare media (Skill work)

Preparation of Starter culture-II-weigh the certified chemicals and prepare the media required for micro organisms and autoclave. Using autoclaved test tubes. Petriplates and prepared media inoculate the culture in Laminar air flow. To prepare sub culture ( Broth media) for different micro organisms weigh the required chemicals and prepare media (Skill work)

After cooling the media, inoculate in laminar air flow, take the suitable amount of cotton to plug the test tubes and flasks and place it in incubator for shaking

Maintenance of Autoclave, distillation unit, Laminar air flow

Wash the glassware using soap, rinse the washed glass wares in distilled water,Dip the glass wares in chromic acid to sterilized, finally rinse in distilled water conical flacks, glass bottles and trays, test tubes, petridish and pipettes

Remove the contaminated media/culture from conical flasks, glass bottles and trays,test tubes, petridish and pipettes, Sterilize them in autoclave and follow the cleaning method

Cleaning and maintenance of Laboratory equipments

Wet mopping of platforms, working table and floor in the laboratory. Then, wet mopping disinfectant/ antibacterial chemicals/ antiviral chemicals, Cleaning of Laboratory corridor. Clean and mopping of soil sieving/ soil preparation room.

Fumigation of Laminar airflow rooms growth rooms and autoclave rooms before and after the production of individual bio fertilizer culture

Cleaning the three fermenters and the semi automatic filling machine and the 600L storage tank twice (once in tap water and once in hot water) in the production unit (before media preparation)

Fumigation of fermenter rooms and packing rooms before productions of individual Biofertilizers

Inoculation of the culture transported from laboratory to the cooled media. specific in fermenters

Filling the bottles with bio fertilizers trough semi automatic filing machine to 1 L bottles

Filling the bottles with bio fertilizers trough semi automatic filing machine to 5 L bottles

Filling the prepared bio fertilizers to half/one/five liters of autoclaved bottles and then place the vented cap and lid to the bottles and arrange the bottles in a proper manner ( Frequent cleaning in case of leakage)

Print the date of production date of expiry batch number in labels (which contain information about bio fertilizer) Stick the printed labels to be bottles

The labeled bottles are transported from production site and assembled in a cooler place

Transportation of prepared product from production site to sales counter (only is case of necessity)

The bottles to be transported each district / taluk must be placed in a carton box and sealed. Assemble the carton boxes particular to the district / taluk (Bottles) (Sending only for delivery under schemes)

Filling the boiler with water and mainenance of pressurised equipments like fermeters and air compressors

General cleaning and fumigation of fermeter,room,mixing and packing area and godown.Cleaning of mixing machine,weighing scales etc

Receiving and cleaning the litter, stone in the soil samples, Sieving the soil, sample and fill into the glass containers and numbering

Drying and powdering of leaf samples, sieving, numbering and preparing the samples for analysis

Filling and numbering the water samples into the respective glass wares , drying filling and numbering of organic manure samples into the respective glass wares

The glass wares which are used for the various parameters were washed once with water, then rinsed with distilled water and finally prepared for different analysis.

Preparation of filter papers for analysis of potash, phosphorus, Micronutrients, Secondary Nutrients and heavy metals

To analyze PH and EC, prepared samples are weighed and added in beaker measured distiled water is added in the beaker and stirred well with a glass rod Calibrate the PH and EC meter with buffer solution and record the readings Soil Samples

o analyze PH and EC, prepared samples are weighed and added in beaker measured distiled water is added in the beaker and stirred well with a glass rod Calibrate the PH and EC meter with buffer solution and record the readings of Water Samples

To analyze PH and EC, prepared samples are weighed and added in beaker measured distiled water is added in the beaker and stirred well with a glass rod Calibrate the PH and EC meter with buffer solution and record the readings of Organic Manure Samples

To analyze Phosphorous, add weighed samples into the labelled glass wares and mix with measured reagents and keep in shaker. Weigh and prepare the chemicals needed for analysis. Filter the samples after the specified time. Then record the reading on the calibrated UV spectrophotometer. Prepare the Graph of Standard Phosphorus. Prepare standards for phosphorus analysis Soil Samples

To analyze Phosphorous, add weighed samples into the labelled glass wares and mix with measured reagents and keep in shaker. Weigh and prepare the chemicals needed for analysis. Filter the samples after the specified time. Then record the reading on the calibrated UV spectrophotometer. Prepare the Graph of Standard Phosphorus. Prepare standards for phosphorus analysis Organic Manure Samples

To analyze potash, weigh the samples I and add to the labeled conical flasks and then add the reagent in the shaker. Weigh and prepare the chemicals needed for analysis. Filter it after the specified time and then record the reading on the calibrated flame photometer. Prepare the Potash Standards of Soil Samples

To analyze potash, weigh the samples I and add to the labeled conical flasks and then add the reagent in the shaker. Weigh and prepare the chemicals needed for analysis. Filter it after the specified time and then record the reading on the calibrated flame photometer. Prepare the Potash Standards of Organic Manure Samples

To analyze organic carbon, weigh the samples and chemicals required for analysis. Label the glassware and add the samples and mix with chemicals for analysis. Soil Samples

To analyze organic carbon, weigh the samples and chemicals required for analysis. Label the glassware and add the samples and mix with chemicals for analysis.Organic Manure Samples

Weigh the soil samples for nitrogen analysis and add into the kjeldahl distillation tube with required chemicals and titrate

For analysis of Nitrogen, weight and mix the acid and add the leaf sample for digestion in a kjeldahl distillation tube on a hot plate. Add required chemicals and tirate

For analysis of Nitrogen, in organic manure samples weigh and mix the acid for digestion on a hot plate, place it in a kjeldahl distillation tube with required chemicals for distillation and titrate

To analyze sodium in water samples, prepare the standards and record the readings in a flame photometer

To analyze chloride in water samples, prepare chemicals, label the glass wares and titrate with required chemicals and record the reading

To analyze bicarbonate in water samples, prepare chemicals , label the glass wares and titrate with required chemicals and record the readings

To analyze micronutrients, Prepare standards , weigh the sample and put them in labeled glassware and mix with required chemicals and keep it in shaker, After specified time remove the glassware from shaker and filter it. Analyze the sample in AAS/ ICPOES Soil Samples

To analyze micronutrients, Prepare standards , weigh the sample and put them in labeled glassware and mix with required chemicals and keep it in shaker, After specified time remove the glassware from shaker and filter it. Analyze the sample in AAS/ ICPOES of Leaf Samples

To analyze micronutrients, Prepare standards , weigh the sample and put them in labeled glassware and mix with required chemicals and keep it in shaker, After specified time remove the glassware from shaker and filter it. Analyze the sample in AAS/ ICPOES of Organic Manure Samples

To analyze secondary nutrients, Prepare standards, weigh the sample and put them into labeled glassware and mix with required regents and place them in the Orbiter Shaker. After specified time remove the glassware from shaker and filter it and record the reading in UV spectrophotometer / AAS/ ICPOES of Soil Samples

To analyze secondary nutrients, Prepare standards, weigh the sample and put them into labeled glassware and mix with required regents and place them in the Orbiter Shaker. After specified time remove the glassware from shaker and filter it and record the reading in UV spectrophotometer / AAS/ ICPOES of Leaf Samples

To analyze secondary nutrients, Prepare standards, weigh the sample and put them into labeled glassware and mix with required regents and place them in the Orbiter Shaker. After specified time remove the glassware from shaker and filter it and record the reading in UV spectrophotometer / AAS/ ICPOES of Organic Manure Samples

To analyze boron, prepare reagent and buffer solution. Weigh the sample and put them into labeled glassware mix. With required chemicals and record the reading on the UV spectrophotometer / ICPOES. Prepare a standard graph for boron analysis of Soil Samples

To analyze boron, prepare reagent and buffer solution. Weigh the sample and put them into labeled glassware mix. With required chemicals and record the reading on the UV spectrophotometer / ICPOES. Prepare a standard graph for boron analysis of Leaf Samples

To analyze boron, prepare reagent and buffer solution. Weigh the sample and put them into labeled glassware mix. With required chemicals and record the reading on the UV spectrophotometer / ICPOES. Prepare a standard graph for boron analysis of Organic Manure Samples

Cleaning and maintenance of laboratory equipment

Washing and cleaning laboratory rooms and platforms

Loading / unloading of contaminated / used bottle for sterilization in autoclave along with waste media disposal (Nos).

Overnight hot water chemical soaking of baby jar bottles and caps, consolidated 4 times washing of bottles along with caps. ( two time machine and tow times hand )later shifted to sterilization ( Nos)

Pre washed bottles and caps are washed with distilled water and loaded to autoclave for Sterilization later bottle caps are oven dried after cleaning with cotton swab dipped in surface sterilizer (Nos)

Material for daily lab activities ( two time washing of Test tube Conical flask or Forceps OR Bladeholder with the help of brush in hot water then sealed in PP cover). (Filling up of cotton filter paper in the bottles OR PP covers, sealed and arranged in the media storage room after sterilization in the autoclaves(Nos)OR

Preparation of methanol solution in prescribed quantity(Ltr)

Washing of Lab coats, mask, caps slippers, Vacuum cleaning of sterile room, weighing of chemicals, fumigants for fumigation of lab and other minor cleaning etc( (one time),

Chemical ,detergent washing and drying of material , utensils used during media preparation (cooker, utensil, beaker, trolley, trays(one time)

Loading unloading of media filled bottles in autoclave for sterilization, wrapping and arranging in the media storage room (Nos)

Stock preparations Chemical weighing, Dilution. labeling, filtering storing in refrigerator without getting contaminated ( skilled work)(Ltr)

Preparation of media using stock solution, weighing of other chemicals, PH maintenance, media dispensing, capping, labeling & sterilization( skilled work)

Inspection, Tagging, Selection and procurement of genuine quality ex plant from disease, pest free mother plant, digging, sorting loading unloading and primary cleaning with water including ex plant cost(skilled work) Banana ( G-9, Robusta & Cavendish) sucker

Inspection, Tagging, Selection and procurement of genuine quality ex plant from disease, pest free mother plant, digging, sorting loading unloading and primary cleaning with water including ex plant cost(skilled work)Yelakki , Red banana,Nedran,Rasabale(sucker)

Inspection, Tagging, Selection and procurement of genuine quality ex plant from disease, pest free mother plant, digging, sorting loading unloading and primary cleaning with water including ex plant cost(skilled work) Others

Weighing of chemicals ,preparing solutions for overnight soaking of ex plant in Antifungal & Antibacterial solution ( Skilled work) (Nos.) Banana

Weighing of chemicals ,preparing solutions for overnight soaking of ex plant in Antifungal & Antibacterial solution ( Skilled work) (Nos.) Others

Prepared ex plant are washed , treated for 4 to 5 times and trimmed Layer by layer for 2 times using (antibacterial , antifungal),surface disinfectants antioxidants, bleaching agents and sterile distilled water. Final washing in LAF using sterilents and inoculation labeling (skilled work) ( Nos) Banana

Prepared ex plant are washed , treated for 4 to 5 times and trimmed Layer by layer for 2 times using (antibacterial , antifungal),surface disinfectants antioxidants, bleaching agents and sterile distilled water. Final washing in LAF using sterilents and inoculation labeling (skilled work) ( Nos) Others

Inspection and detection of contamination in culture bottles ,media bottles for sub culturing in inoculation room from media storage and growth room (Skilled work).(Nos)

Sub culturing,wrapping, labeling, arranging of cultured bottles in growth room ( skilled work) Banana ( G-9, Robusta & Cavendish) sucker(Nos)

Sub culturing,wrapping, labeling, arranging of cultured bottles in growth room ( skilled work) Yelakki , Red banana,Nendran, Rasabale(sucker)(Nos)

Sub culturing,wrapping, labeling, arranging of cultured bottles in growth room ( skilled work)Test tubes

Inspection , identification, Recording , disposing of defective , off type , contaminated culture bottles in the growth room (Nos).(Skilled work)

Inspection ,identification, Recording , dispatching of fully grown ready to harden rooted plants to the washing area. ( No. of bottles) (Skilled work)

Two to three times washing of rooted plants dipping antifungal solutions. Grading, sorting followed by transferring them to the primary hardening chamber (number of plants)

Maintenance and management of Autoclave, Boiler, Millipore, Water softening unit. Generator ( using log book). Hot Air Oven, Rotary shaker, Growth room UV lights,Air conditioning and other equipment (Skilled work) ( Per hour)

Cleaning of tissue culture laboratory and corridors(Sq.m.)

Daily cleaning of inoculation, R & D and other rooms, floor, chair, trolley, laminar equipments and spraying of chemicals to the walls of sterile rooms. (one time)

Every day switching on the UV lights of LAF for surface sterilization, cleaning and made ready for usage

Cleaning and maintenance work of water storage tank, sump ( 1 time per month)

Preparation and filing the potting mixture in pro trays followed by the planting rooted plants in the pro trays, labeling and arranging the trays in the constructed poly tunnel in the greenhouse( Number of Plants)

Watering the pro tray plants using rose head can ( Number of Plants)

Transplanting of primary hardened pro tray plants to poly bag, arranging the bags on the raised bed poly tunnels covered with polythene mulch (skilled work) ( Number of Plants)

Watering the poly bags secondary hardening plants,using house pipe( Number of Plants)

Shifting and arranging tissue culture plants from poly house to shade house (Nos).

Sorting ,grading , rearranging of well secondary hardened ready to plant poly bag plants in trays(No. of plants)

Green House Maintenance ( Side Rolling Opening and Closing, Maintenance of Foggers, Fan and Pads,ground watering.(1 time)