EOI FOR ISOTHERMAL CALORIMETER

Tata Memorial Centre

Advanced Centre for Treatment, Research and Education in Cancer

Technical Specifications Of: Isothermal Calorimeter

Sr No Scope of supply Quantity COMPLIANCE

Technical Specifications / Scope of Supply

1 "Isothermal Calorimeter", complete as per below mentioned configuration and specifications: 1 No.

2 Latest model with state of the art technology should be quoted and supplied.

3 Complete, Ready to Use System should be supplied, which should include control unit,wash module, syringe with tower filling and assemblies,injection syringes, and other start-up accessories required and necessary softwares modules/accessories , Computer system,etc. and all other items required to fulfill tender specifications.

4 The system should be able to directly measures heat released or absorbed during biochemical binding events, from which it should calculate binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS).

5 The system should be a configurable, low volume, highly sensitive isothermal titration calorimeter which should offer the convenience of unattended operation. It should deliver direct, label-free in-solution measurement of all binding parameters in a single experiment.

6 Typical applications should include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.

7 Hardware and Performance specifications needed:

8 Measuring principle:Direct measurement of heat released or absorbed during a binding event with the heat compensation, detection via power feedback.

9 Internal temperature control system:Peltier controlled

10 Adiabatic chamber for sample and reference cell: Cell should be enclosed in adiabatic chamber (impermeable to environment).

11 Temperature range: approx. 4°C to 80°C ± 0.2°C or better in performance

12 Temperature stability :±0.005 °C at 25 °C or better in performance ( i.e. lower in number)

13 Cell Configuraton : Coin-based Or Cylindrical or non-capillary

14 Cell material: Must be corrosive-resistant.Should be made with Hastelloy - or- any other suitable material suitable for applications listed at sr. no. 3,4,5. It should be highly resistance to extreme pH (2-12) conditions,organic slovents,detergents,etc.

15 Cell volume: 350 μL or lower

16 Sample detectable heat range: on lower side:50 ncal or less to on higher side:100ucal or less.

17 Response time :User selectable response times with minimum time should be 10 seconds or lower.

18 Stirring speed:it should have multiple mixing speed options with max speed upto 200 rpm or higher.

19 Binding constants detectable range: Sub-milimolar to pico-molar range.

20 Automated operation with software control required for all fillings, injection, and cell cleaning functions, Pipetting, Wash Module.

21 Injection syringe volume: 250 µL or less

22 Injection volume precision error: +/- 2 microliter or lower

23 Equilibration time : 6 min or lower at 25°C to 5°C

24 User selectable feedback mode: Multiple feedback mode option should be available.

25 Sample concentration range: 12ug or lower

26 Noise level : Noise level measured should be less than 0.34 ncal/s.

27 Equipment Control, Data Acquisition and Data analysis software Features:

28 User friendly handling. Easy to operate interface.

29 Software controls all real-time parameters of the microcalorimeter operation such as capable of running instrument, injector control, allows precise and flexible user control of all auto-pipette syringe loading, dispensing and stirring functions, cleaning of cell and injection syringe, etc.

30 Analysis software should offer batch evaluation of large data sets, automated assessment of data quality and a streamlined user interface that guides the user to final figures and presentation quality graphs quickly and easily. The software should allow assessment of binding interactions that are complicated by inaccurate protein concentration, an inactive protein fraction and/or inaccurate concentration or heterogeneity of compound solutions. Publication quality graphics. Should provide offline analysis software and it should not require a separate software supporting license.

31 Experimental results should get displayed/exported in Excel for further analysis or data export. Data analysis reports should get generated.

32 Computer and Software

33 System should be supplied with a dedicated PC for equipment control, programming, etc. Relevant control software should be supplied. Latest PC configuration should be supplied. 1 No.

34 Specification for computer systems: Latest compatible control computer, DDR RAM 8 GB, HDD: At least 1 TB SATA, DVD writer, high end Graphics card, if needed ,Gigabit Ethernet, latest compatible Windows OS enterprise version , sufficient USB 2.0 and USB 3.0 ports, HDMI ports, 24 inch TFT monitor. ( Provide quoted configuration in the technical bid) 1 No.

35 Licensed version of latest compatible Microsoft office ( Academic version) should be supplied. 1 No.

36 Following accessories should be supplied along with the system:

37 Additional injector syringes/Syringe assembly 2 Nos

38 Syringe Filling 2 Nos

39 Syringe 1ml leur LOK or substancially equivalent 5 Nos

40 Installation, Commissioning, testing and Training

41 Unpacking and Shifting the consignment to the installation site is to be included in the scope of supply. Bidder/manufacturer/authorized service provider should take responsibility to lift/shift the consignment from unloading site to the installation site . Unloading site shall be "Stores Department, KS Building, ACTREC Campus". If needed, Bidder has to arrange for the laborers at no charge to ACTREC. ( Before submitting the quotation, bidders may visit ACTREC to know unloading site and installation site)

42 Installation,Commissioning and Training is included in the scope of supply. Bidder, Manufacturer and/or its authorized representative should undertake installation and commissioning of the equipment. Complete system should be installed, tested for its performance as per manufacturer's SOP/guidelines and demonstrated to the Institute's Users. In depth training should be provided to the Institute's users for maintenance,usage and applications.

43 Input power supply requirements: 240 V AC ± 10 %, 50 Hz, single phase

44 Warranty: 2 years comprehensive on-site- Factory warranty- for the entire equipment. All third-party items, accessories/attachments, etc., integrated with the system should also cover a 2-year warranty from respective manufacturers. Bidder/authorized service provider should provide preventive visits-2 per year, calibration with supply of standard twice a year during PM visit, and breakdown visits as neede during the warranty period without any additional cost.

45 AMC/after sales service-labour only: Quote for the AMC, 5 years after the completion of warranty, which should include service and labour support for 5 years after completion of warranty. AMC Service should also be extended for all third-party items, accessories/attachments, etc., supplied with the system and integrated with the main equipment. AMC should cover 2 PM visits per piece of equipment per year, calibration with the supply of standard, plus breakdown visits as needed. Remote support also should be included.