e-Tender for Procurement of Real Time-PCR, Automated Tissue Extraction Machine and other accessories under Animal Resources Development Deptt, Government of Tripura

Amount

Real Time PCR System with PCR tubes-- System should have 96 well blocks with 0.2ml tubes, Strips and 96 Well PCR Plate - The system should have LED Excitation with at least 5 LED filters for Excitation and 5 for Detection Filter, with 5 color multiplexing. The system should have - Ramping rate of the machine should be 4°C/Sec with temp. - Accuracy: ± 0.1°C; Temp. - Uniformity: ±0.3°C (tested at 55°C); Temperature range: 4-105°C with 0.1°C min increment - Ramping rate : 4°C/Sec (Max) - Gradient Temp. Range 1-36°C. - Automatic 3D hot lid technology to reduce evaporation. Hot Lid temp. - Range 30-110°C (Adjustable Default 105°C), Automatic Hot lid. - Sample Volume should be 5-100 µL. - The detection system should be bottom-based Photo Multiplier Tube Technology (PMT). Where Individual target can be scanned. - Scanning Mode: Designated Line Scan or Entire Plate. - not require any Normalization method. It should be factory-calibrated. No Passive reference should be needed. - Customization of dye should be possible in the system. i.e., the addition of dye to the existing library should be possible - Dyes Used in System should be : F1: FAM, SYBR Green I;F2: VIC, HEX, TET, JOE,TAMRA;F3: ROX, TEXAS- RED;F4: Cy5, Quasar-670; F5: Cy5.5,Quasar-705. - Absolute Quantification Relative Quantification, SNP Analysis, Data Automatic Analysis, Multiple file gene expression analysis, Melting Curve Genotyping, HRM, Customized parameters. - Software to calculate Δct, ΔΔct and RQ fold values after completion of relative quantification assay for automatic post-data analysis and bar chart - Compatible with reagents and plastic ware of any make/company. - plus/minus assay. SNP (should be capable of automatic and manual call with simplified Scatter plot). - Capability to import Standard curves in multiple experiments - Capability to import and export experimental data in text or excel format for data analysis - Provision with software with no download restriction. - Software capable of being downloaded on multiple computers/desktops/laptops. - Presence of local technical application support within 24 hours - The provision of providing the IQOQPQ documents at the time of installation at free of cost along with instrument operation training.

Single Channel Micropipette (0.1-2.5 µL, 0.5-10 µL, 10-100 µL, 20 - 200 µL 100-1000 µL)-- Variable volume microlitre pipettes wherein volumes can be continuously adjusted by rotating the plunger or thumb button. It has a click-stop digital system, reverse pipetting technique and separate, streamlined tip ejector. Each pipette has to be tested & calibrated and should come with its own calibration certificate