Procurement of equipments, appliances under the SERB DST sponsored research projects for the Deptt of Vety Pathology, College of Veterinary Sciences and AH, R K Nagar

Horizontal Gel Electrophoresis System with Power Pack Assembly along with all accessories- 1. A midi horizontal electrophoresis system should be able to run the gel size of 15 x 7 cm, and 15 X 10 cm. The system should be supplied with 15 X 10 & 7 x 7 cm gel trays with Gel tank with safety lid. 2. The supplied gel trays should be UV proof and the trays can be directly kept on the UV Transilluminator and should have an integrated fluorescent ruler in the tray. The ruler should get illuminated on exposure to UV Light for easy and safe calculation of the band movements. 3. A system should include tape free gel casting module for leak free operations. 4. A system should include four1.5mm combs, 8, 15, 20& 30well-fixed height combs each. 5. A system should have the option for adjustable height combs with comb holders. 6. Migration rate of Bromophenol Blue dye should be similar to 4.5cm/hr (at 75 V). 7. A system should have a lid with the safety banana Jacks, which breaks the circuit when the lid is running. 8. Should be provided with a bubble leveler for even gel casting. 9. The gel caster should have 3 height adjustable screws for balancing the uneven platforms for uniform gel casting. 10. The electrodes should be color coded to remove the confusion of wrong orientation. 11. The Lid should have a safety option so that the lid cannot be closed in the wrong orientation. 12. The Lid should have a integrated cables to connect it to the power pack directly. 13. System should be supplied with one branded 20 liter micro oven Power Supply Basic- 1. Programmable power supply should be capable to operate four electrophoresis units simultaneously for four identical runs with graphic LED display. 2. The output range should be 10-300 V, 0.4-400 mA, 1-75 W. 3. Constant voltage, current or Power with Automatic crossover 4. Memory storage: 9 programs, 9 steps, Timer Control: 99 hr, 59 min 5. Automatic Power up after Power failure, Safety features: 6. No-load detection; sudden load change detection. 7. Warranty: Minimum One year 8. Brand: SARTORIUS/BIORAD/ANALYTIC JENA

Deep Freezer(-20°C)-- 1. Capacity (Liters) : 340 Upright Freezer, 2. Temperature (Chiller/Freezer) :-16°C ~ -24°C 3. Dimension (W x D x H inch):24 x 25 x 73 4. No. of Shelves : 7 5. No. of Door: 1 6. Type: Solid 7. Energy consumption – Units/24hrs: 0.89 8. Castors: Yes 9. Lock: optional 10. Frost Free 11. Digital microprocessor controller 12. Quick freezing function 13. High temperature visual alarm 14. Extremely low power consumption -A+ 15. Tropicalized at 43°C 16. Right hand hinged reversible door 17. Sealed drawers – prevent frost loss from open door 18. 80 mm thick insulation. 19. Warranty- 1 year 20. Brand:ELANPRO/CELFROST/COOL PHARMA

UV Mini-Transilluminator-- 1. Dimensions, mm: 247 x 132 x 48.5 (D x W x H) 2. Viewing surface, mm: 150 x 80 (D x W) 3. Wavelength, nm: 365 4. UV tubes: T5 6 W UVA 5. Power: 12 V, 1.5 A 6. Weight, kg: 1.4 7. Compatible gel type: mini gels 8. Compatible gel size, mm: 50 x 60, 105 x 60,125 x 60 9. Temperature: operation 0~40°C, 10. storage –10~70°C 11. Humidity: operation 20~80%, 12. storage 10~90% 13. Transportation conditions: temperature –10~70°C, 14. humidity 10~90°C 15. Altitude conditions: up to 2,000 m 16. Warranty: Minimum-One Year 17.Brand:BIORAD/BECKMANCOULTER/ANALY-TIC JENA

Vortex Shaker-- 1. Visual indication marker for max speed with attachments 2. Adjustable speed range up to 2800 RPM with an accuracy of +/-5% 3. Small footprint saves valuable bench space and can also be used inside hoods and cold rooms 4. 3-way switch for touch, continuous & stand-by mode for unmatched convenience 5. IP21 compliant design makes it spill safe 6. Max Speed: 2800 RPM 7. Orbital diameter: 4 mm 8. Motor type: AC Motor 9. Max Load Capacity: 500 gm 10. Speed accuracy: ± 5% 11. Different modes Touch, Stand-by & Continuous mode: Touch, Stand-by & Continuous mode 12. Dimensions :205 x 136 x 138.5 mm 13. Power Supply: 220 – 240VAC, 50Hz 14. Ambient temperature: 5 to 400C 15. Weight: 3 kg 16. Warranty-1 year 17. Brand: NEAUTION/SARTORIUS/BECKMAN- COULTER

Refrigerated Micro Centrifuge With Variable Rotors- 1. Refrigerated with 24 x 1.5/2.0mL rotor with Click Seal biocontainment lid. 2. Exceptional acceleration of up to 21,000 x g in as little as 12 seconds. 3. Outstanding corrosion resistance with lightweight engineered polymer rotors. 4. Standard rotor runs 24 microcentrifuge tubes in a single row, from 1.5 to 2.0 mL tubes to minipreps and spin columns. 5. Ergonomic one-click rotor opening and closing, as well as transparent design for added safety and convenience with Click Seal biocontainment lids. 6. Simplify operation with intuitive controls, easy-toread displays and fast one-click centrifuge lid closure. 7. Sleek, lightweight, space-saving design. 8. Capacity 24 x 1.5/2mL 9. Max. Speed 14,800rpm 10. Max. RCF 21,100 x g 11. Hertz 50/60 Hz 12. Voltage 230 V 13. Noise Level 50dbA 14. Temperature Range -9° to +40°C 15. Certifications/Compliance CSA, CE, IVD 16. Type Bench top Centrifuge Includes 24 x 1.5/2.0mL rotor with Click Seal biocontainment lid Description Legend Micro 21R Centrifuge, Refrigerated 17. Electrical Requirements 230V 50/60Hz 18. Refrigerated Yes 19. Run Time 1 to 99min (1min increments); HOLD 20. Standards IEC 61010-1, IEC 61010-2-020 21. Dimensions (HWD) 12.9 x 11.6 x 17.5in. (33.0 x 29.5 x 44.5cm) 22. Warranty: 1 Year 23. Brand: THERMO FISHER SCIENTIFIC/ RACY BIOTECH/HERMLE

Gradient Thermal Cycler + 2kva UPS- 1 hour backup-- 1. Should have a sample capacity of 96x0.2ml tubes, 0.2ml tube strips or universal or standard 1x96-well plate of 8x12 formats with six or more Peltier heating and cooling. 2. System which is not capable of taking plates will not be considered. 3. Should have true gradient capability with Dynamic ramping technology, other technologies apart from gradient will not be considered. 4. Should have the feature of dynamic ramping with identical hold times for all the 8 rows of gradient. 5. Should have a temperature differential range of 1-25degC across the rows. 6. Should have intuitive 5.7” (14.5 cm) touch screen interface which can displays graphics in high resolution for easy programming. 7. The touch screen should be responsive for both gloved and ungloved fingers. 8. Should be capable of running reaction volumes from 1-100ul. 9. Should have a maximum ramp rate of 4 deg. C/second. 10. Should have a temperature range of 4-100 deg C 11. Should have a gradient range of 30-100 deg C 12. Should have a temperature accuracy and uniformity of ±0.5 deg C 13. Should have a memory of >500 programs with further expansion through a USB Flash drive for transfer of files. 14. Should have block and calculated temperature control modes. 15. The software should be capable of exporting Run logs and system error logs 16. Should have quick boot up time of not more than 1 min. 17. Should be quiet in operation. 18. System should have built in library of standard protocols for long PCR, fast PCR, reverse transcription PCR etc. 19. Should have the feature of “Instant Incubation” to keep samples at constant temp for ligation and restriction digests. 20. Should have power save mode. 21. Should be compatible with all kind of plastic consu mables and reagents specially reusable sealing Mats. 22. The vendor supplying the Instrument should also have the capability of supplying cDNA Synthesis Kit, Hot Start Taq Polymerase, plastic ware, and horizontal electrophoresis system with power pack from the same Principal Company. Should be supply 2kva online UPS with 1 hour backup. 23. Warranty- 1 year 24. Brand: EPPENDORF/ BIORAD/ ANALYTIC JENA

BIOLAB EQUIPT PRIVATE LIMITED

BIOAPPS