State Plan -2018-19 - Part C- Microbiological- Procurement of rapid detection system including membrane filtration system for SRI,Nettoor and membrane filtration system for 14 Quality control district labs of KWA

Amount

Supplying Membrane Filtration system for 14 KWA district labs and Rapid Detection system for Bacteriology Lab at SRI,Nettoor

Supply of granulated Chromogenic Coliform Agar as culture medium which complies with ISO 9308-1. The performance test shall be in accordance with current version of EN ISO 11133 & ISO 9308 along with test certificate released through ISO 17025 accredited QC lab ,AOAC & EPA approved. It should comply with the latest version of IS 15185 (1 No contains Bottle of 500gm)

Supply of 100ml graduated , transparent autoclavable funnel (atleast 3 to 4 times reusable) having protective rim and vented lid with mixed cellulose ester/ PVDF membranes of size 0.45µm - 47mm. Funnel material shall be clear with all around level indicators. .It should comply with latest version of IS 15185 (1 no contains 150 nos of funnels & membranes)

Membrane Filtration system with Manifold 3- place and vacuum pump with following specification : The Vacuum pump should transfer filtered liquids directly to waste .Does not require a vacuum flask to be connected between the pump and filtration assembly as direct transfer ensures no handling of liquid waste. The Vacuum Pump should be capable to be used both on work bench and inside Laminar flow hood as well .Complies to the ISO 8199 Standard for Water Quality (general guidance on the enumeration of microorganisms by culture) which describes the conditions for membrane filtration.Vacuum Pump should be maintenance free and comply with Electrical regulations & norms.Tubing should be autoclavable silicone material.Vacuum Maximum 700 mbar .Flow rate (measured with water at 20°C). Minimum 3.5 l/min .Protection type IP64 . Protection Class III .Temperature range of the handled sample +5 °C to +80 °C .Maximal viscosity of the liquid 150 cSt .Manifold to have different filtration heads which shall fit to both reusable and disposable filtration devices.Each component shall be able to remove quickly and easily without the use of tools.Flexible to choose the flow direction, the quick connector and the quick plug fit either end of the manifold pipe. Universal Filter heads for manifold.Material of Construction – 316 L Stainless Steel & Anodized HE30 aluminium.All components shall be autoclavable . It should comply with latest version of IS 15185 . Supplier should provide at least 3 years warranty for the Membrane Filtration system .

Supply of empty 55mm diameter petri plates which is autoclavable glass or polypropylene (1 No contains 10 Plates )

Supply of Rapid Microbial Detection unit for simultaneous enumerating of both of e-coli and coliform in 8hrs using fluorescent technology at SRI .Rapid Detection System Includes Membrane filtration system (Vacuum Pump, Manifold and accessories), Reader ,Camera and PC with following specifications.1.State-of -art Rapid Detection System Reader shall be able to detect and quantify microbial contamination in any filterable sample within 1/3 of the time required using traditional techniques. 2. Shall be based on Non-Destructive , rapid fluorescent based method that enables to grow the microorganisms after they have been stained in order to identify them with any standard ID technique 3.Count and view Colony Forming Units (CFUs) through the reader. 4.It shall also be able to view , count and save the results in computer through appropriate technology 5.The system shall be able to detect viable and culturable microorganisms down to 1 CFU per sample using standard membrane filtration 6. The test results from the system must be comparable with the results of standard methods facilitating validation and integration of the system into laboratory without causing disruption. 7.The system should be compact and durable and fits on standard work bench 8.Validation protocol should be supplied along with instrument. 9.Shall have IQ & OQ and PQ onsite support 10.Environmental Operating Conditions : Temperature 15 °C to 40 °C (59 °F to 104<br>°F) .Humidity < 90 % . Altitude < 3,000 m (9,842 ft.) .11 .Materials of Construction : Reader : Camera Housing to be painted aluminium alloy .All parts like front plate, optical chamber handle etc to be of SS 304L. Optical filter: Glass Light Guard Clear polymethyl methacrylate (PMMA).Computer for viewing and counting colonies should alteast have a intel core i5 processor with clock speed of 2.1 Ghz or better, RAM - 8GB DDR4 or better , 1TB eSATA hard drive, 15.6-inch screen or better, latest Windows 10 operating system .Manifold 3- place and vacuum pump with following specification : The Vacuum pump should transfer filtered liquids directly to waste .Does not require a vacuum flask to be connected between the pump and filtration assembly as direct transfer ensures no handling of liquid waste. The Vacuum Pump should be capable to be used both on work bench and inside Laminar flow hood as well .Complies to the ISO 8199 Standard for Water Quality (general guidance on the enumeration of microorganisms by culture) which describes the conditions for membrane filtration.Vacuum Pump should be maintenance free and comply with Electrical regulations & norms. Tubing should be autoclavable silicone material .Vacuum Maximum 700 mbar .Flow rate (measured with water at 20°C). Minimum 3.5 l/min .Protection type IP64 . Protection Class III .Temperature range of the handled sample +5 °C to +80 °C .Maximal viscosity of the liquid 150 cSt .Different filtration heads shall fit to both reusable and disposable filtration devices.Each component shall be able to remove quickly and easily without the use of tools.Flexible to choose the flow direction, the quick connector and the quick plug fit either end of the manifold pipe. Universal Filter heads for manifold.MOC – 316 L Stainless Steel & Anodized HE30 aluminium.All components shall be autoclavable . It should comply with latest version of IS 15185 .Reagent Kit containing I)1 x reconstitution reagent vial (5 ml) II)1 x fluorescence dye vial (5 ml) III) 1 x staining buffer vial (125 ml) IV) 2 x transfer pipettes V) 1 x plastic storage bag . Rapid Detection System Including Membrane filtration system (Vacuum Pump, Manifold and accessories), Reader ,Camera and PC should have at least 3 years warranty

Supply of Incubator unit for incubation of organisms with chromogenic agar plate set to provide an optimal, homogeneous, temperature uniformity and stability.Temperature range 5 deg C above ambient to 105 deg C .Temperature uniformity plus or minus 0.6 deg C at 37 deg C .Temperature Stability should be plus or minus 0.1 deg C. Temperature deviation over time should not be more than plus or minus 0.2 deg C.Air circulation inside the chamber should be mechanical convection. The incubator should have internal glass door for easy viewing of samples without disturbing internal environment The incubator unit’s chamber capacity should be 178 L or more. The incubator should provide automatic over temperature alarm. The interior surface should be made from AISI 430 /1.4016 grade stainless steel.The shelf system should be easy to fit and remove. The user interface should be microprocessor controlled and should be large vacuum fluorescent display. The units should be stackable without the need for using the tools .2 nos of shelves to be included as standard and should have provision of accommodating at least 13 or more shelves.The unit should be able to operate at 230 V / 50 Hz.The incubator should have RS 232 interface to be able to data log the system parameter with third party software.Data logger to be included with the supply. The incubator should be covered with at least 3 years warranty

Comprehensive Annual Maintenance charge inclusive of supply of spares and labour charge for complete Rapid Detection system including Manifold ,Vacuum pump ,reader ,camera, PC etc beyond the 3 years warranty period in the subject specification for SRI

M/S BIOVISION