LAB MATERIAL SUPPLY

Amount

Methylated spirit -----1. Chemical name: Ethanol denatured + 5% Isopropyl alcohol + 5% Methanol 2. Molecular structure: C2H5OH 3. Molecular wt: 46.07 4. l̥Purity: 90%

Phenol Cristal (Carbolic acid) :1. The chemical name : Phenol 2.The chemical structure C6H5OH 3..Molecular Wt. 94.11 4..Melting point 400C+25.Purity 99.5% 6. Pl note .The critical concentration of Phenol on Carbol fuchsin is 5% 7.Phenol is highly corrosive, handle with extreme care.

Basic fuchsine– C20H20ClN3 ---Chemical name: Pararosaniline hydrochloride, Chemical formula: C20H20ClN3, Mol. Wt: 337.86, Dye Content: Approx.85%-88% (dye content must be mentioned), Colour: Metallic Green.

Methylene blue powder--1.The chemical name: Methylene chloride 2.The chemical structure: C16H18 Cl N3S 3.Molecular Wt. 319.9 4.Dye content should be available on the container.Approximately 82%, actual amount =1gms(.required amount= 1/0.82 = 1.22gms )

Sulphuric Acid --Chemical formula: H2SO4, Mol. Wt: 98.08, Specific Gravity 1.84 Minimum Assay: 98%

Hypochlorite solution (Phenol) --Sodium Hypochlorite solution, Concentration not less than 4%, Packaging -5 Liter/pack

Immersion oil / Liq. Paraffine ---Refractive Index closer to that of glassor1.48,colourless,oderless,transperent, Free from Flourescence in day light with Relative Density of 0.827 to 0.890, Viscocity of 110 to 230mPas, Specific gravity of 0.76 to0.78 at 15.50C Immersion oil supplied by the manufacturer of microscope with refractive index closer to that of Glass or 1.515. OR Liquid paraffin (heavy), refractive index of 1.48, colourless, odourless, transparent, free from fluorescence in day light with relative density of 0.827 to 0.890, viscosity of 110 to 230 m Pa s., specific gravity of 0.76-0.78 at 15.5°C.

Silica Gel ---(Fischer scientific Silica gel 1 X 100 gm) Dry and desiccant silica gel crystals have dehumidifying properties. These are not for food grade items. Blue colour of the crystals fades and turns transparent upon absorbing atmospheric moisture. To reuse, heat the transparent crystals till the blue colour is not restored.

Auramine – O powder--Auramine O:Auramine hydrochloride; (1,1-bis(p-dimethyl aminophenyl) methylenimine hydrochloride) Formula: C17H21N3HCl. H2O ..Mol Wt. 321.85Appearance: Yellow to brown powderPotency (Dye content): approximately 85.0% Absorbance: 435nmAuramine O is a yellow fluorescent dye; very soluble in water, soluble in ethanol; usedto stain acid-fast bacteria in sputum or in paraffin sections of infected tissue

Potassium Permanganate:--Formula: KMnO4 Mol Wt. 158.04Potency: >99% Appearance: Purple solid, dissolves in water to give deep purple solutions. Analytical grade (99% KMnO4); MW 158.04 g/mol; decomposition >240 °C (sold in 500 g bottles). (Note: potassium permanganate is a strong oxidant; it can be replaced by methylene blue or diluted blue ink.)

Hydrochloric Acid--Chemical name- Concentrated Hydrochloric Acid, Chemical formula- HCl, Mol Wt:36.46, Specific Gravity 1.18

Ethanol--Chemical Formula:-C2H5OH, clear, Ethanol-ethyl alcohol colorless liquid and the principle ingredient in Lab reagent .

Distilled water

Lense cleaning paper--Soft, lint-free tissue; approximately 80 mm × 100 mm (sold in blocks of 50 sheets with 10 blocks per pack).

Foot Operated Bin--10 litres with lid, made up of plastic, foot operated

Tissue paper Roll--1 X 20 mtr

Filter paper--Filter paper sheet, 580 x 580 mm (approx.), weight approx. 87g/sq. m, packaging of 100 sheets (Whatman No – 1)

Diamond Marker--6" holder with artificial diamond (hard stone) embedded at one end with screw cap to mark on microscope glass slides.

Broom stick--Material : Bamboo stick, Dry ,Size:- 20cm length X 3mm Width , easily breakable, without powder (1 x 1oo No)

Spirit lamp--Lamps made of stainless steel; volume approximately 85 ml; dimensions approximately 100 mm (diameter) and 65 mm (height); with a wick (cotton wool or metal wire), a method of wick adjustment and a cap.

Electronic Weighing Balance--Capacity : 1500 g, Accuracy : 0.05 g ,Display Type : LED

Parafim Tape 250Fit---For use at –40 °C to 50 °C; 100 mm wide × 58 m long; with dispenser.

Permanent Marker Pen---Fast-drying, black, thick-line (0.8–1 mm) marker pens that can write on most surfaces (even when the surface is cold and wet); ink resistant to water, alcohol and autoclaving

Sessior 6”---Stainless steel (18/8), polished; one blade sharp, one blade blunt; 160 mm long; autoclavable.

Hand Sanitizer (Sterillium)--250ml

Disposable l Golves----Powder-free, latex-free disposable gloves made of vinyl (clear) or nitrile (blue); rated as CE category III (for complex risks) and with a textured surface for slip resistance; ≥0.3 mm thick and ≥240 mm long; stocked in sizes (small, medium and large) appropriate for the laboratory staff (sold in boxes, with 100 gloves per box).

Slide Box Plastic---50 slides bracket x 1 Box

Cotton bundle Absorbent---Absorbent, each roll of 0.5 kg.500 gm

Ice Gel Pack---(100/200gm)

Binocular Microscope Bulb---Binocular Microscope Bulb

water bath--20 litres, with cover; temperature regulation controlled by a microprocessor.

Binocular Microscope fuse

Zip Pouch

Cello - Tape---2 inch or 50mm x 45 metres, Self Adhesive Brown Packing tape : Brown Tape (Manual) Brown, Transparent) : Brown color bopp tape for any industrial application adhesive use, Courier service provider, book and application adhesive purpose use.

Disposable Hand Gloves (Plastic)---1X100(Plastic)

Measuring Cylinders---500 ml (Plastic)

Timer--Features to include spring-driven motor, 0–60-minute timer and a disinfectable plastic housing. Timers to have a sound (e.g. buzzer or bell) to signal time elapsed, a stand and a clip to attach them to laboratory gowns.

Staining Plastic bottle with nozzle---Polyethylene flasks with a narrow neck; with a screw cap, swan neck or jet dispenser; volume required 500 ml will depend on the number of slides stained per day

Measuring cylinders----Made of borosilicate glass 3.3 class A; autoclavable; 2.5 ml graduated.

Micropipette with tips---Volume range: 100–1000 μL

Amber bottle with screw cap & teat---1 lit x 1 No Bottles of 1 litre; made of amber borosilicate glass; GL45 thread and cap; compliant with ISO 4746 (Oxygen-free copper – scale adhesion test).

Conical flask 1 lit---Made of borosilicate glass 3.3; thick walls and narrow mouth; graduated.( 1 lit)

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