ANNUAL RATE CONTRACT FOR GENOMIC SERVICES SUCH AS NEXT GEN SEQUENCING (NGS) GENOTYPING, SYNTHESIS OF GENE, DNA FINGERPRINTING, OLIGOS etc. DURING THE FY-2020-21

Sanger Sequencing

Plasmid 800-1000bp

PCR product 400-800 bp

Plasmid 800-1000bp with plasmid purification

PCR product 400--800 bp with PCR product purification

Genome walking per kbp

Whole Genome sequencing of bacterial/fungi/virus/other microbes/plant/insect (NGS platforms other than PacBio and Nanopore)

High Quality Data minimum of following size (with QC report and raw reads)

800MB TO 2 GB

Whole Genome sequencing of bacterial/fungi/virus/other microbes/plant/insect (NGS platforms other than PacBio and Nanopore)

High Quality Data minimum of following size (with QC report and raw reads,WITH STANDARD BIO INFORMATICS LIKE DeNovo/reference assembly,SSR,SNP, Gene identification etc.)

800MB TO 2 GB

Whole Genome sequencing of bacterial/fungi/virus//plant/animal (NGS platforms other : PacBio)

High Quality Data minimum of following size (with QC report and raw reads)

800MB TO 2 GB

Whole Genome sequencing of bacterial/fungi/virus//plant/animal (NGS platforms other : PacBio)

High Quality Data minimum of following size (with QC report and raw reads,WITH STANDARD BIO INFORMATICS LIKE DeNovo/reference assembly,SSR,SNP, Gene identification etc.)

800MB TO 2 GB

Whole Genome sequencing of bacterial/fungi/virus//plant/animal (NGS platforms other : Nanopore)

High Quality Data minimum of following size (with QC report and raw reads)

800MB TO 2 GB

Whole Genome sequencing of bacterial/fungi/virus//plant/animal (NGS platforms other : Nanopore)

High Quality Data minimum of following size (with QC report and raw reads,WITH STANDARD BIO INFORMATICS LIKE DeNovo/reference assembly,SSR,SNP, Gene identification etc.)

800MB TO 2 GB

Metagenomic sequencing amplicon of 16/18s rRNA (V3-V4) (Raw reads with quality reports and standard bioinformatics analysis)

Metagenomic sequencing amplicon of 16/18s rRNA (V3) (Raw reads with quality reports and standard bioinformatics analysis)

Transcriptome processing and sequencing

Raw reads with quality reports and annotations of following size (with QC report and raw reads, with standard bio informatics analysis like Denovo/reference assembly, SSR,SNP, Gene identification etc.)

minimum 2GB data

Transncriptome processing and sequencing and bio informatics with minimum 2GB data (Raw reads with quality reports with Gene identification, GO, DGE, annotation, phylogenetic analysis, target analysis etc and comprehensive report.)

Small RNA sequencing (Raw reads with quality reports with annotation, phylogenetic analysis, target analysis etc and comprehensive report)

NGS mediated Fungal ITS sequencing and data analysis (library preparation, sequncing, data analysis & Report)

Organelle DNA Sequencing (DNA preparation & Mitochondrial Library QC, 4.5-5 Million PE Raw reads and Data Report)

Bioinformatics Services (Denovo genome analysis/Reference based genome analysis/Hybrid approach/comprehensive Metagenomics/comprehensive 16S rRNA metagenomics/ Metatranscriptomics)

Methylome sequencing and analysis (Sample preparation, qulity test, bioinformatics analyses including annotations, base calling)

Gene scan analysis for SSR merkers or Fragment size analysis using ABI sequencer machine-per 48 samples (Fragment size data efficiency of 1bp difference)

Gene scan analysis for SSR merkers or Fragment size analysis using ABI sequencer machine-per 96 samples (Fragment size data efficiency of 1bp difference)

Genotyping by sequencing (GBS) with each sample data minimum of 1GB data with bioinformatics analysis (with QC report and raw reads. Standard bioinformatics analysis, linkage map QTL analysis etc.)

Meta Transcriptome sequencing (with QC report and raw reads standard bio informatics analysis)

SNP array Design (Design and Deliveryof SNP array)

SNP genotyping per sample (Chromatogram, Fasta File and report with minor allele frequency)

Microsatelite genotyping (Standard report including chromatogram, allele size, peak area and height, heterozygous status)

Gene Synthesis Service

upto 3kb

Oligo Synthesis

Customized guide RNA synthesis

Gene expression studies

Microarray for gene expression pro filing (Standard bio informatics analysis report)

qRT-PCR analysis (Standard analysis report)

Protein Analysis

Protein isolation and pruification (Isolation and purification)

MALDI-TOF analysis of protein sample (Qualitative Analysis)

MALDI-TOF analysis of protein sample (Qualitative and Quantitative Analysis report)

Protein Sysnthesis service

Peptide Mapping and MALDI-TOF/TOF (Data analysis, Report, Mass list, Spectrum Image etc.)

Peptide Mapping and MALDI LC MS/MS with data analysis (Data analysis, Report, Mass list, Spectrum Image etc.)

N-terminal Amino acid sequencing (Amino Acid Sequence)

C-Terminal amino acid sequencing(Amino Acid Sequence)

Metabolome characterization with NMR/GC-MS/ Lipidomics (Data Analysis and Report)

Using NMR System

Using GCMS

Using GCMS-MS

Using LCMS

Using LCMS-MS

Bioserve Biotechnologies India Pvt Ltd

GENOSYS INFORMATICS

BioXplore Labs

Clevergene Biocorp Pvt Ltd

LCGC LifeSciences LLP

Eurofins Genomics India Pvt Ltd

Bencos Research Solutions Private Limited

Xcelris Labs Ltd

Nucleome Informatics Pvt Ltd